Polymerase chain reaction (PCR) technique for the diagnosis of hematological disease brought about revolutionary progress. It has become indispensable and basic technology for genotyping of acute and chronic leukemia and diagnosis of myeloproliferative disorders, thalassemia, hemophilia and other genetic diseases. Based on the PCR technology, multiple nested-PCR, real-time fluorescence quantitative PCR (Q-PCR), denaturing high pressure liquid chromatography (DHPLC), capillary electrophoresis, the molecular hybridization and directly sequencing technique have been developed. The multip]e nested-PCR can be used to examine a variety of leukemia genes and confirm the type of leukemia. The application of Q-PCR has effectively solved the problem of pollution of the PCR and it can be used to monitor minimal residual disease of leukemia. Capillary electrophoresis is more suitable for gene fragment length change. DHPLC can be applied for high-throughput screening of gene mutation. Sequencing is a reliable method and it has been used for routine diagnosis.

Application of imatinib has made a lot of chronic myeloid leukemia (CML) patients longterm survival,but the CML BCR/ABL1 kinase domain mutations (KDM) will result in drug resistance of imatinib.There are many KDM types.The different types appear randomly.The leukemic clone with different KDM characteristics has a certain evolution.In the period of imatinib treatment,a higher degree of drug resistance mutations in cells likely to develop as the dominant clone.It is suggested that the treatment of imatinib-resistant CML in addition to efforts to develop next-generation tyrosine kinase inhibitors outside,but also consider the use of traditional medicine to help cure.

Immunophenotyping with flow cytometry can clearly identify multiple myeloma cells and monitor minimal residual disease by using the characteristic of light chain restriction in plasma cells and some cell surface markers( eg CD138, CD38, CD56, CD117 ). This technology may provide important evidence in the diagnosis of multiple myeloma.

With the fierce competition of higher education market,the public university and college face the pressure from mana-gement toward economic operation.This article tries to make some study on what the economic operation innovation of the public higher education institutions is,to make a synopsis analysis on disadvantageous factors and advantageous factors,and to put forward some suggestions on its promising system.

Thirty-four patients with acne vulgaris were treated with pricking and ear-pressure. Acupoints were Baihui (GV 20), Dazhui (GV 14), Taodao (GV 13), Shenzhu (GV 12), Shendao (GV 11), Lingtai (GV 10), Feishu (BL 13), and Sanyinjiao (SP 6), for priking. Lung (MA-IC1), Stomach (MA-IC),Endocrine (MA-IC3), Subcortex (MA-AT1), EarShenmen (MA-TF1) and Cheek (MA-L) were applied for ear-pressure. After 4 courses' treatments, 33 cases obtained effectiveness and 1 case ineffectiveness.

OBJECTIVEThis study aims to compare the fracture resistance of teeth restored with different post and core systems by cyclic loading test and to predict long-term effects.

METHODSA total of 15 recently extracted mandibular premolars without crowns were endodontically treated. The teeth were randomly divided into three groups with five teeth in each group. Group A, teeth restored with cast metal post and core. Group B, teeth with fiber post and resin core. Group C, teeth with resin filling. All of the teeth were restored with full-cast metal crowns. The specimens were exposed to 300,000 cyclic loadings in a cyclic loading machine and 1,000 thermal cycles between 5 and 55 °C. All of the samples were loaded in AG-IS mechanical testing machine with a compressive load until fracture occurred. The fracture load and the fracture mode of each tooth were recorded.

RESULTSThe fracture resistance in group B and C were higher than that of the cast metal post and core restoration (P < 0.05). Repairable fractures were observed in group B and C, whereas unrestorable and catastrophic fractures were found in group A.

CONCLUSIONFiber post and resin filling is a good choice to restore endodontically treated teeth.

Objective To investigate the effect of the selective cyclooxygenase-2 inhibitor celecoxib on the expression of PGE2 and VEGF induced by IL-1? in A549 cell lines.Methods A549 cells were incubated with IL-1? in the presence or absence of celecoxib.After indicated incubation time,cells were harvested to detect VEGFmRNA by reverse transcription-polymerase chain rection(RT-PCR),the medium was collected to determine the concentration of PGE2 and VEGF by enzyme-linked immunosorbent assay(ELISA).Results After Celecoxib intervention,the formation of PGE2 and VEGF expression decreased more than control group(IL-1? induced),the difference being significance(P

Objective To investigate the distribution,the drug-sensitivity tests and drug-resistance of bacteria in patients with lower respiratory tract infections diseases in respiratory intensive care units(RICU).Methods Retrospective study of the clinical data,the distribution and the drug-sensitivity of bacteria of 178 cases with mechanical ventilation in RICU.The drug-sensitivity were examined by Kribry-Bauer(KB)paper method and minimal-inhibitory-concentration(MIC)by agar double dilute method.Results A total of 326 strains bacteria were obtained in 178 patients.Among 326 strains G-bacteria were the most common kinds of bacteria(78.2%);main kinds of the G-bacteria were pseudomonas areuginosa(30.4%)and baumanii(23.0%);fungus was the second commonest kind of bacteria(16.6%).Most of the G+ bacteria were staphycoccus aureus.In 107 cases more than two kinds of bacteria were obtained.The drug sensitivity test in vitro showed that these strains were multiresistant to commonly used antibiotics,and drugs whose sensitive rate was over 70% included meropenem,impienem,cefoperazone/sulbactam,amikacin and piperacillin/tazabactam,the sensibility of vancomycin to G+ bacteria being 100%.Conclusion Pseudomonas areuginosa,baumanii and fungus are the main pathogens of the patients in RICU;meropenem,impienem,cefoperazone/sulbactam,amikacin and vancomycin are effctive antibiotics for treating lower respiratory tract infection in RICU.There is mixed infection by various bacteria,and some strains are multiresistant.We should monitor drug-sensitivity to bacteria dynamic and use antibiotics reasonably.

Objective: To investigate the effect of oat on blood glucose、insulin and serum lipid in aged patients with type-2 diabetes mellitus.Methods: Blood glucose(FBG,PBG),insulin(F-INS,P-INS) and serum lipid(TG,TC) were determined in eighty aged patients with type-2 diabetes mellitus before and after 50-100 g oat intake. Results: FBG,PBG and P-INS were decreased significantly(P

Thirteen cases of supraglottic cancer extending to various contiguous parts of the glottic region treated with horizontal laterovertical or horizontal anterovertical subtotal laryngectomy and repaired by the use of cervical myocutaneous flap are reported.All 13 patients were living and well up to the date of reporting, among whom. 11 cases had the laryngeal functions (phonation. deglutition and respiration) completely restored following the extraction of the tracheostomic tube while 2 cases had partially restored (phonation and deglutition) with the tracheostomy tube retained.The advantages of the cervical myocutaneous flap for reconstruction of post-operative laryngeal defects were discussed. Its use seems to have widened the indication for subtotal laryngectomy.