The acticle describes a time—resolved fluoroimmunoassay for serum progesterone,based ona competitive solid—phase antigen technique。Progesterone—BSA conjugates are coated onto thewhite opaque microtitration wells。Anti—progesterone antibody is biotinylated.Eu~(3+) are labelledto streptavidin as the tracer.The assay standard curve covers a range of 0.1—100ng/ml.Thelowest detecting limit is 0.05ng/ml。The intra and inter C?V s are 6.45% and 8.93% respec-tively.The average recovery is 89.5%.40woman samples are measured with RIA and presentTRFIA and the results are satisfactory,giving a correlation coefficient of 0.9619.The perfor-mance charicter—istics of the assay are discussed and compared with other known immunoas-says.

Objective To explore the effective communication between therapists and inpatients during rehabilitation treatment. Methods The communication needs were evaluated among 150 inpatients, including stroke and spinal cord injury, following physical dysfunction in 7 aspects to determine the patient's psychological and demand conditions. Then, targeted and effective communication plans were developed and adjusted according to the individual changes of the patients at all stages of the pathogenesis and the patients' feedback information. Finally, the patients' satisfaction surveys were issued when the patients discharged. Results After effective communication was used, 98.5% patients felt satisfied with shortened hospital stay and satisfactory limb function. Compared with the previous patients, effective communication can significantly improve the outcomes and reduce the incidence of doctor-patient disputes. Conclusion Effective communication is helpful for the patients to understand the disease, which can enhance the rehabilitation of compliance and improve rehabilitation outcome.

目的探讨以主动运动为主的社区康复训练对脑卒中恢复后期患者日常生活活动能力的影响。方法将85例社区脑卒中恢复后期患者分为对照组42例和康复组43例。出院后，康复组继续在社区接受以主动运动为主的康复训练，对照组不再进行任何康复训练。结果经过90 d左右，康复组Barthel指数明显提高（P<0.01）。结论以主动运动为主的社区康复训练可提高偏瘫患者的日常生活活动能力。

In Japan, acupuncture and massage does not belong to medical treatment, but a behavior similar to it.Acupuncture and massage along with Chinese medical science suffered tremendous impact since Japan's Meij Restoration.This paper made a summary on the history, development, academy, and current educational situation of Japanese acupuncture and massage industry.

Tumor metastasis, the main characteristic and important indication of malignant tumor, is also the primary cause of death for most cancer patients. The tumor metastasis is a multistep process involving various factors, multiple interactions of many genes as well as the microenvironment. To investigate the mechanism of tumor metastasis will help us understand the essences of metastasis process, therefore exploring molecular targets for clinical diagnosis and treatment of cancer.

To explore the connotation and essence of treatment of winter diseases in summer with analysis and deduction. Treating winter diseases in summer is the concrete embodiment and application of taking advantage of "recuperating yang in spring and summer". Winter diseases are formed by compound factors with deficiency of yangqi as the prerequisite and yin as well as cold as the predominant pathogens. Its pathological characteristic rests with stagnation in meri-dians and collaterals. Aiming at curing chronic diseases, reinforcing yangqi and removing stagnation in meridians and collaterals, treatment in summer is a treating strategy focused on proper opportunity of treatment, which is expected to yield twice the result with half the effort. To select the suitable indications is taken as the core of this treating strategy. And at the same time, blind expansion without careful consideration is not suggested.
Acupuncture Therapy ; Disease ; classification ; Humans ; Seasons ; Time Factors

Purpose To investigate the expression of Lgr5 in cervical squamous cell carcinoma (CSCC) and its significance.Methods Lgr5 protein expression was evaluated by immunohistochemistry of EnVision two-step in 83 paraffin-embedded CSCC specimens,56 normal cervical tissues specimens and 32 cervical intraepithelial neoplasia (CIN) specimens.The expression of Lgr5 mRNA was evaluated by RT-PCR in 13 pairs of surgically removed CSCC and adjacent normal cervical tissues.The correlation between Lgr5 expression and clinicopathological features were statistically analyzed.Results The positive proportion of Lgr5 protein in CSCC tissues was significantly higher than those in adjacent normal cervix (P ＜ 0.01) and CIN (P ＜ 0.05).The expression of Lgr5 in CSCC tissues was associated with grade of tumors (P ＜ 0.01) and depth of invasion (P ＜ 0.05),but not associated with age,tumor size,clinical stage and lymph node metastasis (P ＞ 0.05).Kaplan-Meier analysis showed that the 5-year survival rate of patients with Lgr5 overexpression was significantly lower than that of patients with lower expression (P ＜ 0.01).Conclusion The expression of Lgr5 in CSCC tissues may associated with grade of tumors and depth of invasion and may be involved in initiation and development of CSCC,and it may be a new assistant marker for prognosis of CSCC.

OBJECTIVE:To validate the test condition and test method of the microbil limit test of the Dental ulcer pellicles of Sunset Abelmoschus Flower.METHODS:The methodological validation was carried out in accordance with the standard for microbil limit test stated in the appendix of China Pharmacopoeia(2005 Edition,VolumeⅠ).RESULTS:By membrane-filter procedure,the recovery rates of different test strains in the samples were all over 70%.CONCLUSION:The membrane-filter procedure can more effectively eliminate the bacteriostatic action of Dental ulcer pellicles of Sunset Abelmoschus Flower so as to result in accurate microbil limit test results.

Microwave ablation(MA)has been one of the most quickly developed minimally invasive techniques for trearing hepatocellular carcinoma(HCC)in the last decade.Under accurate imaging guidance,MA shares the common advantages of thermal ablation such as flexible treatment approaches,good tolerability and the ability to consistently create reproducible and predictable necrotic areas.It also exhibits several particular advantages compared with other thermal ablation methods,including higher thermal efficiency,higher capability of coagulating blood vessels,faster ablation time,and an improved convention profile.Over the years,MA equipments,especially the antenna,have been refined for safety and to increase the coagulation zone.With the development of internally-cooled antenna and using the frequency of 915 MHz nficrowave,the technique is becoming safer and the coagulation zone increased significantly.Initially,MA was limited to the treatment of small HCC,but with the improvement of antenna and treatment strategV,large HCC(＞5 cm)can also be ablated effectively.For the"curative"treatment of HCC.the following eriteria need to be met:single HCC with the lesion of 6 am or smaller;three or fewer HCC lesions with a maximum diameter of 4 cm,and absence of portal vein thrombosis or extrahepatic metastases.A five-year cumulative survival rate has exceeded 50%,which is comparable with resection.Moreover,the local immune function is enhanced after MA.A change in immunocyte infiltration takes place in number,configuration and location after patients with HCC are treated with MA.Further research for MA may be focused on the improvement of the maneuverability of the equipment,increase of the accuracy of antenna puncture and consummate the clinical therapeutic standardization.