The acticle describes a time—resolved fluoroimmunoassay for serum progesterone,based ona competitive solid—phase antigen technique。Progesterone—BSA conjugates are coated onto thewhite opaque microtitration wells。Anti—progesterone antibody is biotinylated.Eu~(3+) are labelledto streptavidin as the tracer.The assay standard curve covers a range of 0.1—100ng/ml.Thelowest detecting limit is 0.05ng/ml。The intra and inter C?V s are 6.45% and 8.93% respec-tively.The average recovery is 89.5%.40woman samples are measured with RIA and presentTRFIA and the results are satisfactory,giving a correlation coefficient of 0.9619.The perfor-mance charicter—istics of the assay are discussed and compared with other known immunoas-says.

Objective To explore the effective communication between therapists and inpatients during rehabilitation treatment. Methods The communication needs were evaluated among 150 inpatients, including stroke and spinal cord injury, following physical dysfunction in 7 aspects to determine the patient's psychological and demand conditions. Then, targeted and effective communication plans were developed and adjusted according to the individual changes of the patients at all stages of the pathogenesis and the patients' feedback information. Finally, the patients' satisfaction surveys were issued when the patients discharged. Results After effective communication was used, 98.5% patients felt satisfied with shortened hospital stay and satisfactory limb function. Compared with the previous patients, effective communication can significantly improve the outcomes and reduce the incidence of doctor-patient disputes. Conclusion Effective communication is helpful for the patients to understand the disease, which can enhance the rehabilitation of compliance and improve rehabilitation outcome.

目的探讨以主动运动为主的社区康复训练对脑卒中恢复后期患者日常生活活动能力的影响。方法将85例社区脑卒中恢复后期患者分为对照组42例和康复组43例。出院后，康复组继续在社区接受以主动运动为主的康复训练，对照组不再进行任何康复训练。结果经过90 d左右，康复组Barthel指数明显提高（P<0.01）。结论以主动运动为主的社区康复训练可提高偏瘫患者的日常生活活动能力。

To explore the connotation and essence of treatment of winter diseases in summer with analysis and deduction. Treating winter diseases in summer is the concrete embodiment and application of taking advantage of "recuperating yang in spring and summer". Winter diseases are formed by compound factors with deficiency of yangqi as the prerequisite and yin as well as cold as the predominant pathogens. Its pathological characteristic rests with stagnation in meri-dians and collaterals. Aiming at curing chronic diseases, reinforcing yangqi and removing stagnation in meridians and collaterals, treatment in summer is a treating strategy focused on proper opportunity of treatment, which is expected to yield twice the result with half the effort. To select the suitable indications is taken as the core of this treating strategy. And at the same time, blind expansion without careful consideration is not suggested.
Acupuncture Therapy ; Disease ; classification ; Humans ; Seasons ; Time Factors

Tumor metastasis, the main characteristic and important indication of malignant tumor, is also the primary cause of death for most cancer patients. The tumor metastasis is a multistep process involving various factors, multiple interactions of many genes as well as the microenvironment. To investigate the mechanism of tumor metastasis will help us understand the essences of metastasis process, therefore exploring molecular targets for clinical diagnosis and treatment of cancer.

To eliminate cancer hazards to human health,the development of the therapeutic cancer vaccine has been given more and more attention.By contrast of tradition therapy,cancer immunotherapy take the advantage of being able to achieve target therapy and take modest harm to organism.Here,we first idiscussed the mechanisms of cancer immune escape,and reviewed the design principles of therapeutic cancer vaccine,as well as the latest research progress of therapeutic cancer vaccine and its application.

Objective:To detect the effects of porcelain-fused-to-metal(PFM)crown on the periodontal tissue of the restored teeth,and the relationship between the levels of intedeukin(IL)-1β,aspartate transaminase(AST)and alkaline phosphatase(ALP)in gingival crevicular fluid(GCF)and the health condition of periodontal tissue.Methods:Among 28 patients,34 maxillary incisors with PFM crowns were selected for the study.There were 19 Ni-Cr PFM crowns and 15 Au-Pt PFM crowns.The contralateral homonymy natural teeth were healthy and treated as control teeth.The patients were followed up 6 to 8 months after restoring crowns.The values of IL-1β,AST and ALP in GCF were analysed.Results:The concentrations of IL1β and ALP were significantly higher in GCF of Ni-Cr PFM compared with those of control,whereas no significant difference in Au-Pt PFM group compared with that of control.The level of GCF-AST was higher in Ni-Cr PFM and Au-Pt PFM group than that of healthy control.Conclusion:Ni-Cr PFM crowns have negative influence on periodontal tissue,but Au-Pt PFM have little influence on periodontal tissue of the restored teeth.The activity of IL-1β,AST and ALP of GCF are sensitive to reflect the effect of PFM crowns of different materials on periodontal tissue.

Background and purpose:Small cell lung cancer(SCLC) is a highly aggressive malignancy with a 5-year survival rate of less than 10%.These features suggest the enrichment in cancer stem cells.Our study was aimed to establish a small cell lung cancer cell line from primary cultured from lung cancer tissue,and to identify and isolate cancer stem cells from the early generations of the established cell line.Methods:By using a serum-free medium,lung cancer primary cells were cultured from a small fresh lung cancer cell sample,and a small cell lung cancer cell line was established after passaging the cultured cells.Lung cancer stem cell markers were searched by using flow cytometry analysis to sort through the third or forth generation cells of the established cell line.Biological characteristics of lung cancer stem cells were studied by using the single cell clone formation test,plat colony formation test and cell sphere formation test.Results:A small cell lung cancer cell line was established by primarily culturing a fresh lung cancer sample.The cell line could easily passaged more than 25 generations.When the third or fourth generation cells of the established cell line were checked by flow cytometry,there was a small population of cells that were obviously with CD44 stronger positive(CD44++ cells,5.1%) than the main population cells,which were CD44 weak positive(CD44+ cells).CD44++ cells showed stronger colony formation ability than the CD44+ cells.Furthermore,only the CD44++ cells could form stem cells when a single cell was seeded in a well of 96 well plates.Most importantly,only the CD44++ cells could form cell spheres in ultra low attachment 96 well plates.These results indicated that the CD44++ cells enriched more cancer stem cells in the small cell lung cancer cell line.When CD44 and CD90 antibodies were co-stained,the cells of the established cell line could be separated into 4 populations,i.e.CD44+CD90-,CD44+ CD90+,CD44++ CD90+ and CD44++ CD90-cells.CD44+ CD90+ cells were the smallest population cells in the cell line,with a ratio of about 1.9%.When cultured in ultra low attachment 96 well plates,CD44++ CD90+ cells had the strongest cell sphere formation ability when compared with other population cells,indicating that CD90 might also be a small cell lung cancer stem cell marker.Conclusion:There were cancer stem-like cells in primary cultured small cell lung cancer cell lines,CD44 and CD90,which might mean that they could be lung cancer stem cell markers.

Enamel hypoplasia is a surface defect of the tooth crown caused by a disturbance of enamel matrix secretion.Enamel hypoplasia may be inherited,or result from illness,malnutrition,trauma,or high concentrations of fluorides or strontium in the drinking water or food.Different types of enamel hypoplasia have been distinguished,such as pit-type,plane-type,and linear enamel hypoplasia.Hypoplasia has been related to the intensity and duration of stress events,the number of affected ameloblasts,and their position along the forming tooth crown.Amelogenesis imperfecta(AI) is a heterogeneous group of inherited defects in dental enamel formation,most teeth are affected in both the primary and permanent dentition.The malformed enamel can be unusually thin,soft,rough and stained.The strict definition of AI includes only those cases where enamel defects occur in the absence of other symptoms.Currently,there are seven candidate genes for AI: amelogenin,enamelin,ameloblastin,tuftelin,distal-less homeobox 3,enamelysin,and kallikrein 4.Since the enamel is formed according to a strict chronological sequence,and once formed,undergoes no repair or regeneration.Then the analysis the phenotype of enamel hypoplasia can provide insights of the severity of inherited or environmental stress and the molecular mechanism during the period of enamel formation.

Antisense oligonucleotides is appplicated abroadly in cardiovascular disease.It aims at the target genes involved in molecule mechanisms of disease pathogenesis.This technology reveals good foreground as a kind of clinical drug because of its researchful and therapeutic function in hypertension、coronary disease,complications of heart transplantation and failure.