Thalassemias are a group of inherited disease which is caused by globin gene defects.Identification and quantification of hemoglobin play a very important role for the screening and diagnosis of thalassemia,especially the quantitative analysis of Hb A2 and Hb F.Through past 60 years,the methods for Hemoglobin analysis have been developed from starch gel electrophoresis,paper electrophoresis,cellulose acetate electrophoresis,agarose gel electrophoresis,column chromatography,high-performance liquid chromatography till capillary electrophoresis,Its development closely associates with the research on thalassemia.No matter what kind of methods is chosen,quality control is very important.

The application of radio frequency identification(RFID) and wireless network to neonates' management can effectively avoid occupying infants by mistake.This technology can provide an advanced accessorial instrument in hospital management and upgrade the hospital's professional level.

Objective To study on the effect of joint surgery of anterior-posterior in the treatment of vitreoretinal diseases complicated with cataract.Methods 78 vitreoretinal diseases patients complicated with cataract(86 eyes)were recruited.All the cases accepted vitrectomy and cataract extraction at the same time.The vision improving situation and postoperative complications of the treatment were analyzed.Results After the joint surgery of anteriorposterior,the visual improvement rate was 88.4％(76/86 eyes),and with unchanged vision in 7.0％,and decreased vision in 4.7％.In 4 patients with decreased vision,1 case was a secondary neovascular glaucoma,and 2 cases were macular hemorrhage or hiatus,1 case was a retinal detachment.In phase Ⅰ,72 eyes did not implant artificial lens,63 eyes(87.5％)got postoperatively improved vision,and 14 eyes implanted artificial lens,and all of them got improved vision(100.0％),and the difference was not statistically significant(P ＞ 0.05).The total postoperative complication rate was 10.5％(9/86 eyes).Conclusion Joint surgery of anterior-posterior in the treatment of vitreoretinal diseases complicated with cataract had determined curative effect and lower complication rate,which deserved further study and widely using.

OBJECTIVE:To establish a method for test of bacterial endotoxin on cefalotin sodium for injection by gel clot.METHODS:Cefalotin sodium for injection was diluted in water for determination of bacterial endotoxin and allocated to sample negative and sample positive controls to determine its maximum noninterference concentration;Then bacterial endotoxins were diluted into solutions of different concentrations with the test solutions,and interference test was carried out by using tachy?pleus amebocyte lysate sensitivity compound nucleus.RESULTS:The maximum noninterference concentration of cefalotin sodium for injection was6.68mg/ml;And cefalotin sodium at the concentration of1.67mg/ml did not interfere with the test for bacterial endotoxins.CONCLUSION:The present method can replace pyrogen test with rabbit for examination of bacterial endotoxin in cefalotin sodium for injection.

Objective To understand the total score of health management knowledge demand and self evaluation score of known health management knowledge,in order for hospitals to take more reasonable and effective health management education for patients after discharge.Methods From March to May of 2012,700 patients in a certain three level of first-class general hospital were selected before their discharge,using self-made health management questionnaire for investigation.The total score of health management knowledge demand and score of known health management knowledge among 700 patients with different demographic characteristics underwent statistics.Results After investigation,the results showed that patients with different nationalities,payment method,the average monthly income,family history,residence and intend demonstrated statistical significance in total score of health management knowledge requirement.Patients with different occupation,culture degree and accept frequency showed statistical significance in known health management knowledge.Conclusions According to the current investigation of total score of different health management knowledge demand and known health management knowledge in patients with different demographic characteristics,the hospital should take corresponding adjustment for health management mission to ensure the health management quality of patients after discharge,and improve the health management level of patients after discharge.

Animals ; Humans ; Neoplasms ; metabolism ; pathology ; Signal Transduction ; physiology

Diagnosis, Differential ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Glomerulonephritis, IGA ; diagnosis ; drug therapy ; Humans ; Kidney Failure, Chronic ; diagnosis ; drug therapy ; Medicine, Chinese Traditional ; Nephrotic Syndrome ; diagnosis ; drug therapy ; Phytotherapy

China ; Humans ; Liver ; surgery ; Translational Medical Research

Objective To study the therapeutic methods of cholecystic duct stones. Methods A retrospective analysis of the clinical data of 158 cases of cholecystic duct stones treated in the recent 6 years was made. Results 97 of the 158 cases of cholecystic duct stones were removed by open surgical operation and 61 cases by laparoscopic cholecystectomy(LC).In 49 of the 61 cases treated with LC,the cholecystic duct stones were removed together with removal of the gallbladder, and 12 by duodenoscopy after LC. Conclusions One should strive to extract the cholecystic duct stones while removing the gallbladder. If the stones or stone fragments escape into the common bile duct, they could be removed through a choledochotomy at the time of the open operation ,or by postoparative duodenoscopy in cases undergoing LC. The latter method of treatment is associated with minor trauma, quick recovery and definite therapeutic results.

Objective To explore the relation between serum Hcy,Cys C levels and type2 diabetic macro-vascular complications and its clinical significance.Methods The serum levels of Hcy and Cys C were measured with immunoturbidimetry in 80 cases of type 2 diabetic patients with macrovascular complications and 80 cases of type 2 diabetes without macrovascular complications,and in 80 healthy subjects which was seleted as control.Results Serum levels of Hcy and Cys C of type 2 diabetic patients with macrovascular complications were (26.42 ± 5.75)μmol/L and (2.30 ±0.40)μmol/L,which were higher than those of patients without macrovascular complica-tions for (15.31 ±3.13)μmol/L and (1.51 ±0.27)μmol/L(t =15.17,14.64,all P <0.05);Moreover,which were also higher than those in the healthy control group for (8.86 ±1.62)μmol/L and (0.85 ±0.14)μmol/L(t =26.29, 30.56,all P <0.05).The serum levels of Hcy and Cys C of type 2 diabetic patients with carotid arteries increased were (32.53 ±6.46)μmol/L and (2.83 ±0.52)μmol/L,which were higher than those of patients without carotid arteries for (18.46 ±3.63)μmol/L and (1.65 ±0.30)μmol/L(t =11.64,12.06,all P <0.05).In patients of type 2 diabetic with macrovascular complications,Hcy was positively correlated with serum Cys C(r =0.617,P <0.05). Conclusion sCD40L and YKL -40 may have important role in the pathogenesis of type 2 diabetic patients with ma-crovascular complications,which can be used as a valuable reference target in the diagnosis and monitoring of vascular complications in elderly type 2 diabetes,and it may be one of the important independent risk factors of patients with type 2 diabetes.