Objective To investigate myocardial apoptosis in the septic rats and to evaluate the role of Bcl-2, Caspase-3 in signal transduction of myocardial apoptosis. Methods Sixty Wistar rats were randomly divided into control group (n=30) and septic group (n=30). Either group was then divided into 5 subgroups:3 h,6 h,12 h,24 h and 36 h subgroup. Sepsis model was established by cecal ligation and puncture (CLP). Control group were given the same treatments except CLP. TUNEL method was used to determine apoptosis index (AI). Western blot was applied to determine Bcl-2 and Caspase-3 expression. Results (1) AI value increased progressively after CLP procedure and peaked at 36 h(43.742±2.495), which showed significant difference compared with control group(4.311±0.035 ). There was no significant difference between 24 h and 36 h subgroup (P>0.05). (2) Bcl-2 protein levels significantly decreased after CLP procedure compared with control group (P<0.01 ) and 36 h subgroup was the lowest, but there was no significant difference between 24 h and 36 h subgroup (P>0.05). Caspase-3 levels gradually increased after CLP procedure,and 36 h subgroup was the highest,but there was no significant difference between 24 h and 36 h subgroup ( P>0.05). (3) There was negative correlation between Bcl-2 and AI (r=-0.753, P<0.001). Conclusion Myocardial apoptosis may be one of mechanisms for myocardial injury in sepsis. Anti-apoptosis intervention may be a promising therapy to improve the prognosis of sepsis.

AIM: To establish an analytical method for detecting 14 kinds of amino acid in Wuling Capsule(xylaria) by HPLC-FLD. METHODS: After being derivated in precolumn with o-phthalaldehyde and ?-mercaptoethanol,the amino acids were eluted by gradient elution and were detected with a fluorescence detector (Ex = 340 nm,Em = 450 nm). The mobile phase consisted of 0. 1 mol/L NaH2PO4 ( adjusted to pH 6. 8 by phosphoric acid) and methanol with a flow rate of 1. 0 mL/min,column temperature was set at 30 ℃. RESULTS: The deriva-tives of 14 amino acids with o-phthalaldehyde could be separated with a stable base line within 40 min. The linear range of the detection was 5-200 nmol/mL and correlation coefficients were over 0. 999 0. CONCLUSION: The method is simple,accurate and well reproduced for the determination of amino acids in Wuling Capsule.

Arginine Vasopressin ; Humans ; Motion Sickness

Alcohol Drinking ; adverse effects ; Cardiovascular Diseases ; etiology ; prevention & control ; Health Promotion ; Humans ; Risk Assessment

Objective To study the effect of Yiqimingmu pill on ATPase and LD of retinal ischemia rabbit, and probe into its mechanism. Methods Thirty-two Japanese large-ear white rabbits were divided into four groups, normal group (A group), model group (B group), comparison group (C group) and treated group (D group). Retinal ischemia was made in B, C and D group. After the treatment of one month, testing the correspondent indexes and studying with statistics. Results After the treatment of one month, in B and C group, the content of ATPse was lower, while the content of LD was higher compared with A group. Conclusion Yiqimingmu pill is reliable in the treatment of retinal ischemia, and superior to Buzhongyiqi wan.

To explore the characteristics and treatment of pseudopericoronitis of wisdom teeth of the mandible by clinical analysis of 36 cases.Results showed that the patients of pseudopericoronitis of the wisdom tooth of the mandible were of elder age,mild symptoms, totally impacted in the mandible,resulting in overgrowth of the corresponding third molar of the maxtilla overgrown.The observations indicated that the symptoms of pseudocoronitis of the wisdom tooth of the mandible was the injury inflicted to the soft tissue due to excessive growth of the third molar in the maxilla directly or indirectly.The method of treatment is to extract the overgrown third molar in the maxilla,and leave the wisdom tooth of the mandible alone.

Objective To disclose the molecular mechanism of calycosin-7-O-β-D-glucoside (CG) accumulation in Astragalus membranaceus, we cloned PAL genes and analyzed the expression patterns of them and changes of CG contents in different tissues of A. membranaceus. Methods PAL genes were cloned with the methods of homology cloning and RACE technique using the total RNA as template and the analysis of bioinformatics on the cloned genes was carried out, gene expressions in root, stem, and leaf were determined with real-time PCR method, and CG content in root, stem, and leaf were analyzed by HPLC methods. Results Three PAL genes were cloned from A. membranaceus. The genbank accession number was KY086279 (AmPAL1), KY086280 (AmPAL2), and KY086281 (AmPAL3), respectively; The full-length cDNA of them was 2 508 bp, 2 401 bp, and 2 498 bp, respectively; And they all consisted of 2 157 bp open reading frame encoding 718 amino acids. Deduced AmPAL proteins had typical active sequences of PAL proteins, they were homology with other PAL proteins, and they shared the highest identities with PAL proteins of leguminous plants. Phylogenetic tree analysis showed AmPAL1 belonged to the different sub-class with the sub-class of AmPAL2 and AmPAL3. Real-time PCR analysis indicated that expression levels of AmPALs were different from each other, the expression level of AmPAL1 was the highest, the expression level of AmPAL2 was the next, and that of AmPAL3 was lowest in all detected tissues, and only the expression levels of AmPAL2 was similar to the changes of CG contents in different tissues (root > stem > leaf). Conclusion The cloned AmPAL1, AmPAL2, and AmPAL3 from A. membranaceus were typical genes of PAL, each might have different function in developing of different tissues, and AmPAL2 might involve in CG accumulation in different tissues.

Objective To assess the stresses of fiber posts combined with auxiliary posts in anterior teeth with flared canals by three dimensional finite element method .Methods Nine 3D models of maxillary first incisors with flared canals restored by two post systems(porcelain pile ,fiber and auxiliary pile) divided into 5 groups were generated .By static loading method ,dental crowns cut 1/3 and middle 1/3 connection link five points into 135 angles by 100 N force loading .The major stress for the dentin and the e‐quivalent Von Mises stress for resin cement and post and core were calculated .Results Biggest tooth stress value of 111 .9 Mpa and the largest resin adhesive stress value 54 .6 Mpa had appeared in the use of a model of the quartz fiber post after repair ,ceramics pile repair model of pile nuclear stress value and the maximum was 48 .1 Mpa .Combining with one auxiliary post ,stress values re‐duced significantly in dentin ,in cement and in post and core .However ,as more auxiliary posts were added ,the stresses did not de‐crease sequentially .Conclusion It is suggested that to restore the tooth with flared canals by quartz fiber post combining with one auxiliary post .

0.05 ).An excellent correlation exists between the two methods (Y= 0.99 X- 0.01 ,r= 0.97 ,P

Background: There are obvious changes in neuropeptides from plasma and cerebrospinal fluid in patients with vascular dementia (VaD), and regulating the levels of neuropeptides is a key for prevention and treatment of VaD. Objective: To observe the clinical efficacy of moxibustion at head-points in treatment of vascular dementia (VaD), and assess its effects on memory-related neuropeptides. Design, setting, participants and interventions: A total of 65 VaD patients from Acupuncture Hospital, Anhui University of Traditional Chinese Medicine, were randomly divided into moxibustion group (33 cases) and Western medicine group (32 cases). Patients in the moxibustion group were treated with indirect moxibustion with common monkshood cake for 20 min. Patients in the Western medicine group were orally administered piracetam tablets, 0.8 g for three times a day. One treatment course was 4 weeks, and they were treated for 4 treatment courses. Main outcome measures: The scores of Hasegawa's Dementia Scale (HDS), Mini-Mental State Examination (MMSE), and Activity of Daily Living Scale (ADL), as well as the levels of learning and memory-related neuropeptides from cerebrospinal fluid such as somatostatin (SS) and arginine vasopressin (AVP) were measured before and after treatment in the two groups. Results: Total response rate was significantly higher in the moxibustion group than in the Western medicine group (P<0.01). There were significant differences in scores of HDS, MMSE and ADL between before and after treatment in the two groups(P<0.05 or P<0.01). After treatment, the scores of HDS, MMSE and ADL in the moxibustion group were more improved as compared with those in the Western medicine group (P<0.05 or P<0.01). The levels of SS and AVP after treatment were higher than those before treatment in the two groups(P<0.01). After treatment, the increased levels of SS and AVP were higher in the moxibustion group than in the Western medicine group (P<0.01). Conclusion: Moxibustion is effective in improving the clinical symptom scores and regulating the levels of neuropeptides associated with learning and memory in VaD patients.