Objective:To evaluate the human pulp response following direct pulp capping with Clearfil SE BOND (SB). Methods:45 sound human third molars in 24 volunteers were used. Pulp of 41 molars was mechanically exposed and then the teeth were divided into two groups: in group A the pulp was capped with SB, in group B the pulp was capped with calcium hydroxide (CH), another 4 teeth were served as the control. After 7, 30 and 90 days, the teeth were extracted and processed for light microscopic examination. Results:7-30 days after capping slight inflammatory reaction was observed in group A and group B. The reaction in group A was sligter than that in group B(P

Objective To detemine the effect of autografts of in-vitro cultured corneal limbal stem cells for treating pterygium. Methods A total of 11 cases (12 eyes) with pterygium were treated with autograft of in-vitro cultured corneal limbal stem cells and were followed-up for 6-15 months. Results During the follow-up pterygium recurred in 1 eye (8.33 %) . All of the grafts survived well and no immunological rejection or other complication occurred. Conclusion Autograft of in vitro cultured corneal limbal stem cells is an effective and valuable method to treat pterygium.

Objective To develop-the rat model of diffuse brain injury(DBI)and to observe the pathophysio logical alteration of the rats after.traumatic injury.Methods A modified Marmarou's traumatic device W88 used to establish the animal model of diffuse brain injury with different degree through changing the impact condition that was applied on the rat brain(including 80cm 450g,120 cm 450g and 180cm 400g);the animal model was evaluated by observing the variations of nervous system signs,vital signs,sensorimotor function,and morphological changes of brain tissue in the rats that have received different impacts.Results The significant changes in nervous system signs,vital signs and sensorimotor function had been observed in all rats that were subjected to the different impact.The pathologic changcs of DBI in rats brain could he found in all groups.Moreover,this characteristic changes would have became more significant following the traumatic iaiury when the impact energy was increased.Conclusion The rat model of graded diffuse brain injury have been developed successfully.The moderate impact(120cm 450g)could produce low animal death rate and result in obvious pathologieal alterations in the injured rat brain.This impact condition would be suitable to be used in the study about DBI.

Objective: To investigate the changes of sugar chain structures of serum amylase and difference in TCM syndrome patterns in primary hepatic cancer (PHC) patients and their relation with free radicals. Methods: Agglutinin precipitation assay was used to detect the binding ratios of serum amylase with various kinds of agglutinin in PHC, hepatocirrhosis, and hepatitis patients. The serum amylase activity and malindialdehyde (MDA) level were determined simultaneously. The association of the binding ratios of amylase with free radicals was analyzed. The difference mentioned above in PHC patients with different TCM syndromes was analyzed. Results: The binding ratios of serum amylase to ConA, PSA, PNA, and LCA were significantly higher in PHC and hepatocirrhosis patients than hepatitis patients and normal controls. The binding ratios of serum amylase to PSA and LCA were significantly higher in PHC patients with spleen deficiency and liver stagnation than those with liver and kidney Yin deficiency. PHC patients with spleen deficiency and liver stagnation had higher ConA-binding ratio compared with those with QI and blood stasis. A positive correlation was found between PSA-, LCA-, and PNA-binding ratios of serum amylase and MDA. Conclusion: For PHC and hepatocirrhosis patients, core-fucosylated high-mannose-type and hybrid-type sugar chains of serum amylase increased. The reduced terminal sialic acid and fucose on the sugar chain caused the exposure of the terminal galactose residues. In addition, the exposure of the terminal GlcNAc residues was induced by decreased terminal galactose on the sugar chain of serum amylase from HPC patients. These changes of serum amylase were also observed in hepatocirrhosis patients. It may be related with the damage of sugar chains induced by free radicals. In spleen deficiency and liver stagnation group, core-fucosylated high-mannose-type and hybrid-type sugar chains of serum amylase increased, and the terminal galactose on the sugar chain decreased, resulting in the exposure of the terminal GlcNAc residues. The changes were not observed in QI and blood stasis or liver and kidney Yin deficiency patients. It indicated that spleen deficiency and liver stagnation played an important role in generation of aberrant sugar chains of serum amylase for PHC patients.

ObjectiveTo study the correlations between Berg balance scale (BBS) and balance subscale of Fugl-Meyer test (FM-B), and evaluate their practicability for assessing balance function of patients after stroke.Methods68 stroke inpatients were assessed with BBS, FM-B and BPM before training. After training they were re-assessed by the same researcher. The parameters of BPM were sway index, sway tract length and area. The patients were assessed twice with eye opened and eye closed respectively.ResultsThere was no significant difference in the score of FM-B between the first assessment and after training (P>0.05), while that of BBS and BPM after training were better than before the training (P<0.001). There was moderate correlation between FM-B and BBS (r=0.517,P<0.001), but there was no correlation between FM-B and BPM (r=-0.074～-0.247,P>0.05). There was moderate negative correlation between BMP and BBS (r=-0.410～-0.587,P<0.01).ConclusionBBS and BPM are good tools for assessing balance function of stroke patients; FM-B shows low sensitivity and is not suitable for assessing stroke patients.

Objective To study the influence of center of gravity excursion in standing on function outcome of stroke patients.Methods46 stroke patients were assessed with static posturography in standing,Fugl-Meyer Balance Scale,Barthel Index and the time in hospital.The patients were divided into 2 groups according to posturography results:center of gravity was inclined to the normal side (normal group),center of gravity was inclined to the hemiplegia side (hemiplegia group).ResultsThe ability of balance,walking and activity of daily living(ADL)of the normal group patients were better than that of the hemiplegia group patients,and the in-hospital time was shorter (P<0.001).ConclusionThe center of gravity excursion in standing is highly correlated with functional outcome of stroke patients.

Objective To explore the impact of concentrated teaching, self-help training, intensive training, scenario simulation and other systemic training methods on the health teaching level of community health education staff,and the impact of community health education on cultivation of residents' health behavior before and after training. Methods 60 community doctors and nurses involved in systematic training. Taught concentrated 8 hours every week, and continuous for 4 weeks with medicine, psychology, sociology, pedagogy, aesthetics and production of courseware. Trainees who awarded 80 points or more in examination took part in simulated scene training, which completed a 30-minute design of concentrated health lesson and a 15-minute design of one to one health lesson in written form and multimedia teaching. Teaching effect-site was evaluated by the residents of the community lectures. The impact on cultivation of residents' behaviors was evaluated with ruler evaluation method.Results The community health care workers' teaching ability had significant change after training: plan design (2.32 ± 1.41 vs 4.26 ±0.61 ), lectures (2.63 ±0.89 vs 4.09 ±0.93), teaching skills ( 1.97 ± 1.32 vs 3.89 ±1. 13 ), teaching aids configuration ( 1.68 ± 1. 43 vs 3.97 ± 1. 26 ), educational methods ( 2.01 ± 0. 96 vs 4.03 ±0.82), time control (2.83 ±0.26 vs 4.67 ±0.25), the classroom atmosphere (2.78 ± 1. 13 vs 4.12 ±0.67),courseware ( 2.48 ± 1. 08 vs 3.89 ± 1.02 ) , teaching the image ( 2.15 ± 1.15 vs 4.06 ± 0.78 ), overall evaluation (2.36 ± 1.21 vs 4. 16 ± 0.65 )(P ＜ 0. 0 1 ). After training, community residents' knowledge on community health care workers' one to one health education and collective health education training, and positive evaluation of helpful to students' behavior improved significantly ( x2 = 17.19,36.37 ;8.91,20.98 ;34.14,32.29) ,and all had remarkable difference( all P＜0. 01 ). Evaluating the impact of health care workers' teach on cultivating residents'health behavior after one year, it improved significantly (P ＜ 0. 01 ). Conclusion Systemic training can improve health education ability of community medical staff and the impact on cultivating residents' health behavior is remarkable.

Objective To investigate the effects and mechanisms of adrenomedullin (ADM) and adrenomedullin receptor (ADMR) on hypoxia pulmonary hypertension (HPH) in rats.Methods Eighteen male Wistar rats were randomly divided into three groups:control group, hypoxic group and hypoxic plus ADM group.ADM was subcutaneously administered into rats with ADM. After 21 days, The mean pulmonary arterial pressure (mPAP) and right ventricular hypertrophy index[RV/(LV+S)] were measured. Hypoxic pulmonary vascular remodeling(HPSR) was observed with morphometric analysis. The level of pulmonary arterial plasma ADM was measured by radioimmunoassay. The semiquantitate in situ hybridization was used to measure the expression of ADMR mRNA in pulmonary artery walls. Results (1) The level of mPAP, RV/(LV+S),the ratio of vascular wall thick ness to external diameter(MT%) and ratio of vascular wall area to total area (MA%) were significantly higher in hypoxia group than those in control group(2) The pulmonary artery plasma ADM level was significantly higher in hypoxic group and hypoxic plus ADM group than that in control group(P

OBJECTIVE To norm the isolation and management of special infection patients and eliminate the incidence of hospital infections.METHODS The measures,the implementation of training and personal management,and details of control were determined.RESULTS The system management and isolation measures were implemented to effectively control the incidence of hospital infections.CONCLUSIONS The isolation and control of special infection patients are a systematic and rigorous work,it is very important to form a scientific,complete and easy to implement workflow and management system for the control of hospital infections.

Objective To acquire the physiological parameters such as pulse,blood oxygen saturation and ECG to realize personal health information monitoring and tailored medical service.Methods A personal health information acquisition terminal was developed,which was composed of an information acquisition module,personal computer and system software.The information acquisition module was developed with USB interface and unified circuit mode.Health information was transmitted to the personal computer at real time with USB interface,and then stored by system software and uploaded to the database.Results The terminal gained advantages in design,convenience and practicability,which realized realtime acquisition,data storage,remote auxiliary diagnosis,printing and etc of personal health information,so that personal information realtime monitoring was implemented.Concluslon The terminal can be used for health information recording,storage,acquisition and analysis,and extends medical service of hospital.