Five complexes of cis- dichloro (Morpholine) (amine) platinum (II) including [Pt(morpholine). (a-naphthylamine)Cl2], [Pl(morphiline)(p-phenetidine)Cl2] have been prepared. The structure of the complexes have been determined on the basis of elemental analysis, molar conductivity measurement, IR and Raman spectral studies. The compounds were tested for biological activity and found to be capable of inhibiting the growth of human two cancers.
Physiological Effects of Drugs ; Chemical synthesis

By condensing azometines of vanilline and aromatic amines with acetophenon, synthesized 7 b-Aminocetones. The structure of obtained products have been characterized by elemental analyses and IR, UV spectroscopy. The compounds have been tested for antibacterial and antifungal activities. Result b-Aminocetone of vanilline have great effect in antibacterial and antifungal activities.
Chemical synthesis ; Physiological Effects of Drugs

4(3H)-quinazolinone derivatives were synthesized by the condensation of N-acetylanthranilic acid with aromatic amines or heteroaromatic amines, respectively. The obtained compounds were characterized by their melting point, elemental analysis and the mass, ultraviolet, infrared, 1H and 13C NMR spectra. The obtained derivatives of 4(3H)-quinazolinone were also biologically screened such as: hypnotic, analgesic, antibacterial and cytotoxic activity. 3-(2-hydroxy-3-pyridinyl)-2-methyl-4(3H)-quinazolinon (6) showed potential analgesic activity. No compounds showed hypnotic, cytotoxic and antibacterial activity.
Chemical synthesis ; Physiological Effects of Drugs ; N-acetylanthranilic acid

Nitration of furfural to give 5-nitrofurfural diacetate (NFD). By the condensation of NFD with 2-butanone (or acethylacetone), two cetone a, b-ethylenic deriva-tives (I,II) were formed. Condensation of furfural with acetone to give (XII). Condensation of I (or II or XII) with various H2N-B compounds to give 15 derivatives (III-XI, XIII- XVIII). The structure of the obtained products were characterized by elemental analysis and IR and 1H-NMR spectroscopy.The synthesized compounds were tested for biological ethylenic activities such as antibacterial and antifungal. Among these, two cetone a, b-ethylenic derivatives of 5-nitrofurfural (I,II) showed a high antibacterial activity on 10 strains of bacteria and a high antifungal activity against Candida albicans.
Chemical synthesis ; Physiological Effects of Drugs ; Furaldehyde ; Analogs & derivatives

AIMTo investigate the changes of glucocorticoid receptor (GR) in rat brain regions with chronic immobilization stress and the influence of Chinese herbs.

METHODSWe copied the rat model of chronic immobilization stress (180 min daily, repeated 7 days or 21 days), and characterized the changes of GR in hippocampus CA1, cortex, dentate gyrus via immunohistochemistry integrated image analysis.

RESULTSThe contents of glucocorticoid receptors in hippocampus CA1 , cortex, dentate gyrus were increased and the intensity of immunity reaction was significantly stronger in the model group of 7 days than that in the normal control group (P < 0.05). The contents of GR were significantly decreased and the intensity of immunity reaction was weaken in the model group of 21 days (P < 0.05).

CONCLUSIONCompared with the model group of 21 days, the contents of GR were significantly increased and immunity reaction was intensified in three groups of treatment with Chinese herbs (P < 0.05), in which the group treated with soothing-liver herbs Xiaoyaopowder was the best.

Animals ; Brain ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; Rats ; Rats, Sprague-Dawley ; Receptors, Glucocorticoid ; drug effects ; metabolism ; Restraint, Physical ; Stress, Physiological

AIMTo discuss the effects of restrained stress on cardiac myocyte apoptosis of rats in the whole body, and the effect of anti-stress-induced cardiac myocyte injury treated by Chinese herbs yixinning.

METHODSAgarose gel electrophoresis and TUNEL are used to detect cardiac myocyte apoptosis.

RESULTS(1) When restrained stress 1,2,4 week, the DNA ladder of stress groups was negative, while in situ apoptosis of stress groups increased apparently compared with control group (P < 0.01). (3) The DNA ladder of yixinning groups was negative too, while in situ apoptosis of yixinning groups decreased compared with stress group (P < 0.05). (3) Whereas in distilled water group, the above indices had no statistical significance compared with stress model group (P > 0.05).

CONCLUSIONThe restrained stress can induce cardiac myocyte apoptosis in rats. Chinese herbs "yixnning" can inhibit cardiac myocyte apoptosis, and have functions of anti-stress injury.

Animals ; Apoptosis ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Male ; Myocytes, Cardiac ; drug effects ; metabolism ; pathology ; Rats ; Rats, Wistar ; Restraint, Physical ; Stress, Physiological

OBJECTIVETo observe the clinical efficacy of modified Wuhua Decoction (WHD) on corticosteroid addictive dermatitis (CsAD) and in improving patients' facial skin barrier function.

METHODSSeventy-five patients were randomly assigned to two groups, the 38 in the treated group treated with WHD together with oral administration of levocetirizine tablet, while the 37 in the control group treated with levocetirizine tablet alone in the same way, 30 days as a course. Skin erythema dose (ED) and transepidermal water loss (TEWL) of patients were measured before and after treatment.

RESULTSFive cases in the treated group and 7 cases in the control group were dropped out. The total effective rate was 69.7% (23/33 cases) in the treated group and 10.0% (3/30 cases) in the control group respectively, with the score of objective symptoms reduced from 5.48 +/- 1.60 before treatment to 1.24 +/- 1.62 after treatment and the score of subjective symptoms reduced from 7.06 +/- 1.54 to 1.55 +/- 1.72 in the treated group, while in the control group, the two indexes reduced from 5.57 +/- 1.25 to 3.27 +/- 1.55 and from 6.77 +/- 1.36 to 3.07 +/- 1.36 respectively, showing significant difference between the two groups and the efficacy in the treated group was better than that in the control group (P <0.01). Skin ED decreased significantly in the treated group after treatment, and insignificantly in the control group. TEWL began to decrease on the 15th day in the treated group, while it was unchanged in the control group; on the 30th day, although a decrease was shown in both groups, its reduction was lower in the treated group (P <0.05).

CONCLUSIONWHD has significant clinical efficacy on CsAD, it could reduce the skin ED and quickly recover the injured facial skin barrier function.

Adrenal Cortex Hormones ; adverse effects ; Dermatitis, Contact ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Face ; Humans ; Skin Physiological Phenomena ; drug effects ; Substance-Related Disorders ; complications

OBJECTIVETo evaluate the effect of compound bushen recipe (CBR) in improving the survival state of stress and the overall life span in C. elegans by simulating chronic fatigue syndrome (CFS) under various stress states.

METHODSThe tolerance and the average survival time of adult larvae against heat stress (35 degrees C), oxidative stress (250 microg/mL juglone), and in vivo Abeta protein toxicity (Abeta(1-42) transgenic mutant CL4176) under the intervention of the high (500 mg/L), middle (250 mg/L), and low (100 mg/L) dose CBR were observed. The effect of CBR on the average live time (at 25 degrees C), movement distance in 20 seconds, the frequency of pharyngeal pump in 30 seconds, and the reproductive capability were assessed.

RESULTSCompared with the control group, the survival time of heat stressed C. elegans could be significantly increased in each CBR group (P < 0.01). The survival time of heat stressed C. elegans could be elongated, the protein toxicity be attenuated, and the live time prolonged in the high and middle dose CBR groups (P < 0.01, P < 0.05).The movement distance and the frequency of pharyngeal pump could also be increased in the high dose CBR group (P < 0.01). There was no statistical difference in the reproductive capability among all groups (P > 0.05).

CONCLUSIONSCBR could significantly enhance the stress capacity of C. elegans against internal and external environment, and prolong their lifespan. It did not interfere their normal production, and also could improve the quality of life, thus laying a foundation for further mechanism studies and pharmacological researches on CBR in preventing and treating CFS.

Animals ; Caenorhabditis elegans ; drug effects ; Disease Models, Animal ; Drugs, Chinese Herbal ; therapeutic use ; Fatigue Syndrome, Chronic ; drug therapy ; Longevity ; Stress, Physiological

AIMTo investigate the changes of enkephalin mRNA and prodynorphin mRNA gene expression in rat brain regions with chronic immobilization stress and the influence of Chinese herbs.

METHODSWe copied the rat model of chronic immobilization stress (3 h daily , repeated 7 d or 21 d), and primers of enkephalin or prodynorphin were respectively added for RT-PCR reaction (BETA-actin as inner contrast). Gel image analysis system was used to scan and analyze and odds of optical density of target gene and inner contrast strip were taken as quasi-quantified data.

RESULTSProdynorphin mRNA expression in hippocampus markedly increased in 7 d group (P < 0.01), while enkephalin mRNA prodynorphin mRNA expression in hippocampus markedly increased in 21 d group (P < 0.01). All three recipes were able to decrease the gene expression of prodynorphin mRNA (P < 0.01), and xiaoyao powder as well as sijunzi soup were able to decrease the gene expression of enkephalin mRNA (P < 0.01).

CONCLUSIONThe effects of xiaoyao powder on the above two gene expression was better than jinkuishenqi pill.

Animals ; Drugs, Chinese Herbal ; pharmacology ; Dynorphins ; metabolism ; Enkephalins ; metabolism ; Gene Expression ; Hippocampus ; drug effects ; metabolism ; Male ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley ; Restraint, Physical ; Stress, Physiological

OBJECTIVETo observe the effect of Uighur medicine gu-jing-mai-si-ha tablet (GJMSHT) for treatment of premature ejaculation (PE) and to explore part of its mechanism.

METHODSThe condition of patients was scored by related questionnaire, and the intravaginal ejaculation latency time (IELT) was observed before and after GJMSHT treatment, with the blood levels of nitric oxide (NO) and prostaglandin F2alpha (PGF2alpha) detected in PE patients as well. The results were compared with those in the control group.

RESULTSAfter treatment, the scores of PE and IELT, as well as the levels of NO and PGF2alpha, all increased significantly compared to those before treatment in the treated group (P<0.01), while in the control group, all the parameters were insignificantly changed (P>0.05). Therefore, the difference of these parameters between the two groups after treatment all showed statistical significance (P<0.01).

CONCLUSIONGJMSHT could treat PE effectively, its mechanism is possibly by strengthening the coordination of the related smooth muscles through increasing the blood levels of NO and PGF2alpha, and the endurance of patients to the cavitary effect of prostatico-urethral pressure, thus postponing the arrival of urgent ejaculatory feeling.

Adult ; Dinoprost ; blood ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Ejaculation ; drug effects ; Female ; Humans ; Male ; Nitric Oxide ; blood ; Sexual Dysfunction, Physiological ; drug therapy ; physiopathology ; Tablets