The cross-sectional study was carried out on 4,397 households at Ha Noi city, Ha Tay province and Hai Duong province to investigate the prevalence of traumatic injury. Results: the rates of traumatic injuries were 22.8% in Ha Noi, 16.8% in Hai Duong, and 21.8% in Ha Tay. Injury is a leading cause of morbidity with the rate of 23.42/1,000 people/year, account for 20.4% of all illness. The causes of injuries were traffic accidents (43.6%), following by fall (31.6%). The highest rate was on age group of 50-54 years old (35.8/1000 people). The rate of traffic accidents among males was higher than this of female, and the most cause of traffic accidents was motorcycle accident (56.2%). Traumatic injuries were common in legs (24.0%), then in heads (22.9%), shoulders (18%), feet (17.9%), and arms (19.1%). Almost injuries influenced the diseases and economies of victims and their families.
Wounds and Injuries ; Epidemiology

Retrospective epidemiology and documentary analysis collected from National Medical Investigation to evaluate an alcohol abuse situation and its harmful effect. The results: alcohol abuse is a social evil. It does not estimate and consider adequately from managers, experts and people so far. Alcohol abuse is important role in increasing injuries such as: traffic, violence, poison, suicide and sexual assault. It is necessary to have appropriate methods, sanctions for people, who drinks alcohol while driving and breaks the law
Accidents ; Wounds and Injuries ; Beer ; Alcohols

Background: Prostatic Specific Antigen (PSA), a helpful tumor maker in the diagnosis of prostate cancer, may rise due to many causes such as Digital Rectal Examination (DRE), Acute Urinary Retention (AUR), ect. Objective: To evaluate on the influences of some factors on the serum PSA level in Benign Prostatic Hyperplasia (BPH). Subjects and method: This study included 122 men who were diagnosed BPH with a mean age of 71.18 +/- 7.19 years (ranged 54-92). The patients were divided into 3 groups: DRE group included 60 patients, their blood samples were obtained before and after 24 hours; catheterization group included 35 patients who presented with AUR, their PSA values were determined before 48 hours and 2 weeks after elevation; the inflammation group consisted of 27 patients with symptoms of acute urinary tract infections, the PSA levels were evaluated before 48 hours and 4 weeks after anti-inflammatory therapy. Results: The PSA levels increased significantly in patients with larger prostate sizes (p<0.01, r = 0.492). PSA level tended to increase with age (p<0.05, r = 0.29). The PSA values changed 14.72 +/- 10.85% after DRE (p<0.05). After relief of urethral catheterization, PSA levels decreased 50.77 +/- 20.42% (p<0.05). After anti-inflammatory therapy, 51.59 +/- 21.87% (p<0.05). Conclusions: These results suggested that it may be the best way to perform DRE after obtaining serum PSA for analysis. Serum PSA concentration should not be determined when AUR and acute urinary tract infection.
Benign prostatic hyperplasia ; PSA

Background: Success in preventing HIV transmission from mother to child has been confirmed, however intervention remains difficult in both services and access barriers due to discrimination and stigma. Objectives: To clarify the HIV prevalence in pregnant women and evaluate the indicators related to the impact of intervention on prevention of mother-to child transmission of HIV. Subjects and method: This study was carried out in 5 districts in 5 provinces that implemented the Prevention of Mother to Child Transmission (PMTCT) Project. The interventions included the training to provide knowledge, skills on counseling, testing and supervision on PMTCT. The data were collected from designed-questionaires. Results:The results showed there are 100% of pregnant women that came for antenatal visits, had voluntary test counseling. The prevalence of HIV (+) among pregnant women was 0,43%. There were 57 infants born to mothers with HIV (+); received prophylaxis treatment. At the time of the study, there were 31 infants at 18 months of age that received HIV test and all of them were confirmed as negative. Conclusion: Intervention on PMTCT was effectively achieved and should be expanded nationally. Communication, counseling is very important to encourage pregnant women with HIV (+); visit for antenatal care, followed up by health facility and strict adherence to the PMCT schedule to diminish the chance of transmission to their infants.
Prevention of Mother to Child transmission ; HIV testing counseling ; HIV voluntary test.

Background: Down syndrome is a developmental disorder caused by an extra copy of chromosome 21, is a condition in which extra genetic material causes delays in the way a child develops, both mentally and physically. It affects about 1 in every 700 babies. The fetus having high risk for Down syndrome (OS) can be detected early by ultrasound. Objectives: The aim of the study is to find out some ultrasound markers that relate to OS fetus. Subjects and method: A descriptive study was carried out on 612 pregnant women with fetus \ufffd?12 weeks by ultrasound to detect abnormal markers in fetus. The fetus were diagnosed Down syndrome by analysis chromosome from amniocyte and monitor up to the neonate. Then, finding out association between OS fetus and ultrasound markers. Results: Among 612 pregnant women,36/12 pregnant women had abnormal imaging in fetus, 11/12 pregnant women had OS fetus. There were 12 pregnant women detected OS fetus. 6/12 OS fetus associated with the maker of nuchal skin fold (cut off 2: 3mm at the first trimester and 2: 6 mm at the second trimester): Detection rate (DR) was 50%; false positive rate (FOR): 0,83%. 3/12 OS fetus associated with the marker of duodenal atresia. DR was 25%; FOR: 0%. Conclusions: The two common markers associated with OS fetus: \r\n', u'the first marker was nuchal skin fold (with cut off 2: 3mm at the first trimester and > 6 mm at the second trimester) and the second marker was duodenal atresia. \r\n', u' \r\n', u'
Down Syndrome ; Fetus/ anatomy & ; histology ; abnormalities ; physiopathology ; ultrasonography

Background: Recently Japanese Encephalitis (JE) virus type 1 has surfaced and is co-circulated with JE virus type 3 in the northern areas of Viet Nam, so a sensitivity of JE viral antigen genotype 3 to detect IgM is required. Objectives: To compare the sensitivity of JE viral antigen genotype 1 and 3 to detect IgM against the JE virus. Materials and method: 783 cerebrospinal fluid and serum samples from viral encephalitis cases from 1999-2005 were collected and examined by MAC-ELISA for JE viral antigen genotype 1 and 3. Results: The agreement on the diagnosis of these kinds of antigen was 99.7% and the sensitivity of JE viral antigen genotype 3 was higher than that of genotype 1. Thus, JE viral antigen genotype 3 could be considered as the selected antigen for JE diagnosis in Viet Nam. IgM titer determined by JE viral antigen genotype 1 was higher than that of genotype 3 in 2003 and 2005 and lower in 1999, 2000, 2001, 2002 and 2004. Conclusion: The dominant phenomenon of JE viral genotypes differing over the years might be due to the interaction of the virus and its vectors. Further study is required to clarify this observation.
Japanese Encephalitis ; antigen

Background: In recent year, Japanese Encephalitis Virus (JEV) genotype 1 has been detected among isolates from mosquitoes and pig\u2019s blood samples in northern Viet Nam, but there has been no information on the presence of this genotype in the Central, Southern and Highland regions. Objectives: This study aims to detect the Japanese encephalitis genotype 1 in various different geographic regions of Viet Nam. Material and method: Sequence analysis\u2019s of whole E gene of 18 strains isolated from human, mosquitoes and pig\u2019s blood during 2001-2007. Results: 7 strains isolated from pig\u2019s blood and mosquito samples in the Northern, Central, Southern and Highland fell into genotype 1, but 11 others isolated from humans in the Northern and Central regions belonged to genotype 3. Conclusion: This is the first time that JEV genotype 1 was detected in the central, northern, highland Viet Nam and further studies on genotype 1 causing human diseases needs to be carried out.\r\n', u'\r\n', u'
Virus ; Japanese Encephalitis ; genotype 1 ; E gene.

Introduction: Antiviral resistance has been reported in seasonal influenza A viruses and avian influenza A(H5N1) viruses in Viet Nam, raising concerns about the efficacy of treatment. Methods: We analysed specimens from two sources during the period 2009–2012: influenza-positive samples from influenza-like illness patients at sentinel clinics in northern Viet Nam and isolates from patients with confirmed A(H5N1) infections. Pyrosequencing was used to detect mutations: H275Y [for A(H1N1) and A(H5N1)], E119V [for A(H3N2)] and I117V [for A(H5N1)]. A neuraminidase inhibition assay was used to determine the Inhibitory Concentration 50 (IC50) values for all influenza A and B isolates. Results: There were 341 influenza A positive samples identified; influenza A(H1N1)pdm09 was identified most frequently (n = 215). In 2009, oseltamivir resistance was observed in 100% (19 of 19) of seasonal A(H1N1) isolates and 1.4% (3/215) of A(H1N1)pdm09 isolates. This H275Y mutation was not found in influenza subtypes A(H5N1) or A(H3N2) isolates. Discussion: In Viet Nam, seasonal and A(H5N1) influenza vaccines are not currently available; thus, effective treatment is required. The presence of oseltamivir-resistant viruses is therefore a concern. Active surveillance for oseltamivir resistance among influenza viruses circulating in Viet Nam should be continued.

Background: Mosquitoes and pigs play important roles in maintaining and increasing the Japanese Encephalitis (JE) virus in nature and which is then transmitted to humans. Thus, surveillance of the JE infection frequency in the pig population may predict the human JE cases. \r\n', u'Objectives: The study aimed to determine IgG antibody against the JE virus in the pig population in Hanam province \r\n', u'Subjects and methods: The study included 1791 pig serum samples collected from 3 districts of Hanam province from Apr 2006 to Mar 2007. GAC-ELISA technique was used to determine the JE virus infection in the swine population.\r\n', u'Results: The average positive rate in pig population was 34.9 % (626/1791); with the highest frequency occurring in the summer (37.7%- 84.0 %), co-incident with the JE season in Northern Vietnam. On the contrary, in winter JE case are rare, frequency of IgG antibody against JE virus in the swine population was low, ranging from 9.2% to 22.0.%. \r\n', u'Conclusions: These results have shown the ecologically close relationship between the amplification of the JE virus in the swine population, vector and JE cases in northern Vietnam. \r\n', u'
Japanese encephalitis ; pig population ; GAC-ELISA.

Background: IgM antibody capture ELISA (MAC-ELISA) technique has been widely applied for Japanese Encephalitis Virus (JEV) diagnosis. So far rare internationally commercial kits are available. Thus, the international evaluation of the kit is required as per the recommendation of the WHO. Objectives: To evaluate the quality of the IgM antibody capture ELISA diagnostic kit for JEV produced by the Vietnam National Institute of Hygiene and Epidemiology (NIHE). Subjects and method: In this study, NIID kit was used as control to check the kit from NIHE. Both NIHE and NIID kits were used to detect JEV IgM among 38 serum and 6 CFS samples, which belongs to 5 sample groups (JE patients group, dengue patients group, other viral encephalitis patients group, Tick Born Encephalitis (TBE) patient group and healthy JE vaccinated donors group). Results: The detection of JEV IgM by NIHE kit was concurrent with the NIID kit. There is no positive with the JE in the groups of Dengue patients, TBE, other virus encephalitis patients and JE vaccinated donors. Conclusion: MAC-ELISA kit of NIHE can be used for different diagnosis of JEV and Dengue virus (both viruses are in Flavivirus genus), as well as other viruses caused by encephalitis.
IgM antibody ; ELISA diagnostic kit ; Japanese encephalitis virus