1.Study on the Optimum Conditions For the Expression Of Human Recombinant Proinsulin In E.Coli - BL21(DE3) with pET - 28a(+) Vector
Journal of Medical Research 2008;0(1):56-61
Introduction: There are thousands of diabetes sufferers worldwide. In addition, there is an increased trend in Vietnam due to economic development, increased population, lifespan, and changing lifestyle. Insulin is a hormone, which is a natural protein. It plays an important role in the transformation of glucose in human and animal blood. Note, while insulin has not been produced in Vietnam, the production of recombinant Proinsulin is a premise for insulin. This study is based on a successful design of pET - 28a (+) Vector with recombinant Proinsulin codified gene.\r\n', u'Objectives: To define the human proinsulin codified gene and study the optimum conditions for the expression of proinsulin. \r\n', u'Subjects and method: To discover the appropriate conditions for the expression of proinsulin, including initial cell density, cultural temperature, IPTG concentration, and expression time. The product of the expression was confirmed by Sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SOS - PAGE) and reconfirmed by Western blotting with His - Tag antibody. \r\n', u'Results: Proinsulin expression was successfully proved by SOS - PAGE and Western blotting. Four appropriate conditions for the expression were confirmed as highlighted in the Conclusion. \r\n', u'Conclusion: The appropriate conditions for expression of proinsulin: cell density was 00600 0.6 - 1.0; the cultural temperature was 300C; IPTG concentration was 0.4 mM; the length of the culture time was 20 hours. \r\n', u'
Expression
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Proinsulin
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E.Coli
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Appropriate conditions
2.Blood biochemical parameters and anthropometric indexes among people with risk factors for type 2 diabetes
Phong -- Le ; Binh and colleagues Van Ta
Journal of Medical and Pharmaceutical Information 2003;0(11):26-29
Background: Diabetes is a common chronic glucide metabolic disorder affecting hundreds of millions of people around the world. In Vietnam, the number of people with risk of type 2 diabetes is approximately 5 million. \r\n', u'Objectives: To discover the changes of blood biochemical parameters and mean body mass index (SMIJ waist circumference in individuals with diabetic risk factors). \r\n', u'Method: 95 people with diabetic risk factors were selected by screening. One group was in Ngoc Trao or Phu Son wards (n=52) and other group was in Sa Dinh ward (n=43). \r\n', u'Results: In Ngoc Trao and Phu Son wards: the prevalence of blood glucose, cholesterol triglyceride, HDL, LDL disorders and SMI>23 were 65.3%, 32.7%, 46.2%, 1.9%, 36.5%, 61.5%, respectively. In Sa Dinh ward: those were 62.8%, 29.7%, 44.2%, 2.3%, 34.9% and 67.4%, respectively. \r\n', u'Conclusion: Disorders of blood biochemical parameters accounted for very high rate in individuals with diabetic risk factors and could be considered main risk factors. \r\n', u'
Blood biochemical parameters
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anthropometric indexes
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type 2 diabetes
3. 20-Hydroxyecdysone from Dacrycarpus imbricatus bark inhibits the proliferation of acute myeloid leukemia cells
Trinh Thi THUY ; Nguyen Thanh TAM ; Nguyen Thi Hoang ANH ; Dang Viet HAU ; Tran VAN SUNG ; Dinh Thi PHONG ; Le Quoc THANG ; Sabrina ADORISIO ; Domenico V. DELFINO
Asian Pacific Journal of Tropical Medicine 2017;10(2):157-159
Objective To investigate the anti-proliferative effects of 20-hydroxyecdysone isolated from the bark of Dacrycarpus imbricatus (Blume) de Laub. Methods Column chromatography was used for isolation of compounds from plant material. The structure of the isolated compound was identified by mass spectrometry and nuclear magnetic resonance techniques, including HSQC, HMBC, NOE-difference experiments. The isolated compound was tested for its anti-proliferative activity in acute myeloid leukemia (AML) and OCI-AML cells. Results Compound 1 was isolated from the ethyl acetate fraction of Dacrycarpus imbricatus barks by column chromatography. Its chemical structure was identified as 20-hydroxyecdysone (20HE), a cholestane-type ecdysteroid, by a combination of mass spectrometry and nuclear magnetic resonance spectrometric analyses. Our goal was to test the anti-proliferative activity of 20HE using the OCI-AML cell line. 20HE significantly decreased OCI cell number at a concentration of 1 mg/mL, whereas lower concentrations were ineffective. Moreover, this decrease was due to partial blockage of the G