No abstract available.
Female ; Luteal Phase*

Background and Objective: Blood collection errors are one of the most common causes of laboratory sample rejection in the pre-analytical phase of the testing process. This study aims to determine the frequency and identify the preanalytical factors that lead to rejection of samples meant for the hematology laboratory. Methods: This cross-sectional, retrospective study analyzed blood samples received and rejected by the Hematology Division of the University of the Philippines – Philippine General Hospital from 2018 to 2022. Data were extracted from the Division's annual reports and sample rejection logbooks. The causes and frequency of sample rejections, as well as the hospital locations of the patients involved were presented using frequency tables. Results: Out of 1,072,366 blood samples received during the study period, 61,935 (5.78%) were rejected. The most common cause of rejection was clotted blood samples for both routine hematology (86.31%) and coagulation (44.43%). Clotted samples were the predominant cause of sample rejection across most age groups, with the exception of the neonatal and infancy groups, where inadequate sample quantity was the primary issue. The highest rejection rate was seen in the emergency department (65.71%) and intensive care units (9.68%). Conclusion The rejection rate in our institution was higher than reported in previous global studies. The main causes of rejection were identified as clotted blood samples and inadequate blood volume for routine hematology and coagulation testing. Notably, the highest rejection rates for hematology-related requests occurred in critical areas, including the emergency department, intensive care units, and obstetrics and gynecology.
pre-analytical phase

No abstract available.
Acute-Phase Proteins ; Bacterial Infections*

The study was carried in Chemistry Department of University of Sciences and Entomology Department of National Institute of Malariology - Parasitology - Entomology during the period of 2001-2002. A manual syringe for SPME was made by using a fused silica fiber from optical cable, one of the end of the fiber is passed through the hole of a needle of 5mL medical syringe and is attached to a piston in a protective holder of 1mL medical syringe, and the other end is coated by liquid phase polyacrylate (the fiber end in a 0.1g/mL solution of polyacrylate in dichloromethane). Then the liquid sample to adsort analytes during 30-45 minutes. At the end, the 2 needle is inserted into the GC/ECD injector port is desorbed the analytes on to the column
Syringes ; Solid Phase Microextraction ; Pyrethrins

A restrospective study carried out on 309 patients with chronic myelogenous leukemia treated from 1990 to 10/2000 showed that; -88 cases (28.3%) converted to the blast crisis. - 89.77% of these cases developed acute myeloblastic leukemia (AML) and 9.09% developed ALL.-The percentage of male and female were 55.68% and 44.31% - 45.45% of these converted cases occurred in the age group of 30-50. The youngest age was 10 months and the oldest age was : 72 years old. Mean of duration of the chronic phase was 2.5 years.
Leukemia, Myeloid, Chronic ; Leukemia, Myeloid, Chronic-Phase

Fever has been recognized as a cardinal feature of disease since antiquity, but only recently has the pathophysiology of fever come to be understood. It became clear that the ultimate cause of fever is not a bacterial product (a so-called exogenous pyrogen) but a product of host inflammatory cells (i.e., an endogenous pyrogen). Many studies have demonstrated that mononuclear phagocytes are the principal source of endogenous pyrogen and that a variety of mononuclear cell products can mediate the febrile response. Cytokines are also important as mediators of the acute-phase response to infection and inflammation.
Acute-Phase Reaction ; Cytokines ; Fever* ; Inflammation ; Phagocytes

No abstract available.
Female ; Luteal Phase* ; Reproductive Techniques, Assisted*

Our hospital began to deliver treatment of the sort which could well fall into line with the newly classified set of good clinical practices in June 1998, and our department began to provide in Norvember 1999. This report provides the results of a survey on the clinical drug trials we have since done. The number of clinical drug trials which had been done at our department before September 2005 and for which the period of registrationhad come to an end was 13. One hundred and sixty-one patients were contracted for clinical drug trials. Of them, consent was secured from 138 patients. The number of registered patients came to 125, and the rate of performance stood at 77.6%. A phase-specific comparison of this rate reveals that Phase II trials in the former period accounted for 25%, Phase II trials in the latter period 90.0%, Phase II/III trials 86.7% and trials for long-term asministration 81.3%. When the performance of clinical drug trials was compared in terms of rate on the basis of whether placebos were involved, the rate was 75.9% for six trials not including placebos and 78.5% for seven trials including placebos. For one trial for an acute disease, the rate was as low as 37.5%. The rate of performance was relatively high at our department, but when it comes to Phase II trials in the former period and trials for acute diseases, the rate of performance was low. The existenceor nonexistence of placebos did not affect the rates of performance.
Clinical ; Performance ; Phase ; Pharmaceutical Preparations ; Acute Disease

The teaching of clinical communication skills to undergraduate medical students in the Faculty of Medicine, University of Malaya is described. It is a continuous process throughout the five-year medical curriculum which is divided into Phases I, II and III. Students are introduced to communication skills early in Phase I through an interactive session as well as a workshop on general communication skills. In Phase II, small-group two-day workshops cover the basic principles of clinical communication skills using videotapes, group discussion and role-plays. Direct contact between students and patients in actual clinical setting begin in Phase IIIA. Communication skills teaching with feedback training is carried out by videotaping the consultations. In Phase IIIB the two-way mirror is utilized as well as having workshops on certain difficult areas such as 'breaking bad news' and 'taking a sexual history'. Formal assessment is done by evaluating the behavior, language and actual interview content.
skills ; Communication ; Phase ; Teaching aspects ; Clinical

BACKGROUND: This study aimed to evaluate the adhesion of Acanthamoeba trophozoites on cosmetic contact lenses (CLs) with and without CL care multipurpose solution (MPS) treatment. METHODS: Acanthamoeba lugdunensis L3a trophozoites were inoculated onto disks trimmed from CLs: 1-day Acuvue moist, 1-day Acuvue define, Acuvue 2, and Acuvue 2 define. After 18-hour inoculation, the number of adherent trophozoites was counted under phase contrast microscopy. The effects of MPS, Opti-Free Express, soaking CLs for 6 hours, on Acanthamoeba adhesion were analyzed. Scanning electron microscopic examination was performed for assessment of Acanthamoeba attached on the lens surface. RESULTS: Acanthamoeba trophozoites showed greater adhesion to cosmetic CL (P = 0.017 for 1-day CL and P = 0.009 for 2-week CL) although there was no significant difference between the types of cosmetic CL. On all lenses, the number of adherent Acanthamoeba was significantly reduced after treatment with MPS (P < 0.001 for 1-day Acuvue moist, P = 0.046 for 1-day Acuvue define, P < 0.001 for Acuvue 2, and P = 0.015 for Acuvue 2 define), but there was still significant difference between conventional and cosmetic CLs (P = 0.003 for 1-day CL and P < 0.001 for 2-week CL, respectively). More attachment of Acanthamoeba was observed on colored area and the acanthopodia of Acanthamoeba was placed on the rough surface of colored area. CONCLUSION: Acanthamoeba showed a greater affinity for cosmetic CL and mostly attached on colored area. Although MPS that contained myristamidopropyl dimethylamine reduced the adhesion rate, there was a significant difference between conventional and cosmetic CLs.
Acanthamoeba ; Contact Lenses ; Microscopy, Phase-Contrast ; Trophozoites