1.Effects of visible light on cultured bovine trabecular cells.
Fagang JIANG ; Fengqin HAO ; Houren WEI ; Desheng XU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(2):178-184
To explore the biological effects of light on trabecular cells, cultured bovine trabecular cells were exposed to visible light of different wavelength with different energy. Cellular morphology, structure, proliferation, and phagocytosis were observed. The cells showed no remarkable changes when the energy was low. When the exposure energy reached 1.12 mW/cm2, the cytoplasm showed a rough appearance, and cell proliferation and phagocytosis decreased. This phototoxicity was strong with white light (compound chromatic light), moderate with violet light or yellow light, and mild with red light.
Animals
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Cattle
;
Cell Proliferation
;
radiation effects
;
Cells, Cultured
;
Light
;
Phagocytosis
;
radiation effects
;
Trabecular Meshwork
;
cytology
;
radiation effects
2.Effects of visible light on cultured bovine trabecular cells.
Fagang, JIANG ; Fengqin, HAO ; Houren, WEI ; Desheng, XU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(2):178-80, 184
To explore the biological effects of light on trabecular cells, cultured bovine trabecular cells were exposed to visible light of different wavelength with different energy. Cellular morphology, structure, proliferation, and phagocytosis were observed. The cells showed no remarkable changes when the energy was low. When the exposure energy reached 1.12 mW/cm2, the cytoplasm showed a rough appearance, and cell proliferation and phagocytosis decreased. This phototoxicity was strong with white light (compound chromatic light), moderate with violet light or yellow light, and mild with red light.
Cell Proliferation/radiation effects
;
Cells, Cultured
;
*Light
;
Phagocytosis/radiation effects
;
Trabecular Meshwork/cytology
;
Trabecular Meshwork/*radiation effects
3.Molecular mechanisms of low intensity pulsed ultrasound-mediated cellular behavior in human primary macrophages.
Yi-Ping LI ; Shao-Xia ZHOU ; Andreas SCHMELZ ; Max G BACHEM
Journal of Experimental Hematology 2007;15(6):1253-1256
The aim of this study was to explore the molecular mechanisms of the effect of low intensity pulsed ultrasound (LIPUS) on human primary macrophage functions. Macrophage phagocytosis was analyzed using fluorescein isothiocyanate (FITC)-labelled Escherichia coli (E.Coli); focal complex and extracellular matrix metalloproteinase inducer (EMMPRIN) were observed by fluorescence microscopy; the secretion of metalloproteinases (MMPs) was examined by gelatin zymography, and the expressions of EMMPRIN and extracellular signal-regulated kinases (ERKs) were detected by Western blot. The results indicated that LIPUS accelerated macrophages to phagocytose E.Coli (29.81+/-0.36 vs 18.00+/-0.78), promoted the protein expressions of EMMPRIN and MMPs, increased the level of protein tyrosine phosphorylation, and induced the phosphorylation of ERKs. Furthermore, the above functions were only found in adherent macrophages, and were inhibited or decreased by mitogen activated protein kinase kinase (MAPK kinase, MEK) inhibitor PD98059 and RGD (Arg-Gly-Asp peptide), one of main integrin recognition sequences. It is concluded that the effect of LIPUS on macrophages depends on cell adhesion, and relates to integrin-MEK-ERK pathway.
Basigin
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metabolism
;
Extracellular Signal-Regulated MAP Kinases
;
antagonists & inhibitors
;
metabolism
;
Humans
;
Macrophages
;
cytology
;
immunology
;
radiation effects
;
Matrix Metalloproteinases
;
metabolism
;
Phagocytosis
;
radiation effects
;
Phosphorylation
;
Ultrasonics