The peritoneum is a biologic semi-permeable membrane. This article presents some kinetic models of fluid and solute trans-peritoneal transport in peritoneal dialysis including the membrane model, the three-pore model, the extended three-pore model and the distributed model. In these models different trans-peritoneal transport mechanisms were revealed. The three-pore model is emphasized.
Animals ; Biological Transport ; Humans ; Kinetics ; Models, Theoretical ; Peritoneal Dialysis ; Peritoneum ; blood supply ; metabolism

The three-pore model is a lumped parameter mathematical model and was proposed to investigate the trans-membrane mass transfer in peritoneal dialysis. The model predicted trans-membrane transport of volume and solute reasonably well. We reviewed respectively the previous home and abroad research on three-pore model. The model's computer simulations in clinical application as well as its possible limitations were presented.
Biological Transport ; physiology ; Computer Simulation ; Humans ; Models, Biological ; Peritoneal Dialysis ; Peritoneum ; blood supply ; metabolism

This study was performed to investigate the effect of peritoneal glucose load on plasma leptin concentrations in the continuous ambulatory peritoneal dialysis (CAPD) performed on 13 non-diabetic ESRD patients. Plasma leptin and insulin concentrations were measured for 2 hours during a single 2 liter exchange of 1.5% glucose-based dialysate (SPD, n = 6), for 7 days of daily peritoneal dialysis (DPD, n = 7). In DPD, standard full volume (2,000 ml x 4 times/day) exchange was performed immediately after operation. In SPD, plasma leptin and insulin concentrations remained unchanged during the study. In DPD, the plasma leptin concentration increased significantly after CAPD on the first day (PD1) (11.2 +/- 5.4 to 17.0 +/- 6.0 ng/mL, p < 0.05) and this elevation seemed to persist until 7 days after operation. After CAPD, there was no significant day-to-day variation in peritoneal glucose absorption (391-465 cal). Oral intake seemed to decrease on operation day (PD0) and PD1 and then increased slowly. Plasma insulin and glucose concentrations did not significantly change after CAPD. Changes of leptin concentration were significantly correlated with the changes of peritoneal glucose absorption at PD1. In conclusion, continuous peritoneal glucose load may affect plasma leptin concentrations in CAPD patients.
Adult ; Aged ; Female ; Glucose/metabolism* ; Human ; Leptin/analysis* ; Male ; Middle Age ; Peritoneal Dialysis, Continuous Ambulatory* ; Peritoneum/metabolism*

OBJECTIVETo study the effects of advanced glycosylation end products (AGEs) on the production of fibronectin (FN) in human peritoneal mesothelial cells (HPMC) in vitro and the role of protein kinase C (PKC) in this course.

METHODSThe AGE-human serum albumin (HSA) (0, 100, 500, 1 000 microg/ml) was used in culture medium to stimulate the HPMC. The mRNA level of FN was measured with real-time PCR, moreover, the protein level of FN in HPMC was detected by ELISA. With the method of ELISA, the PKC activities were observed. Inhibitors or activators of PKC were used to observe the roles of PKC pathways on the AGE-HSA stimulated productions of FN in HPMC.

RESULTSAGE-HSA activated PKC in HPMC in a dose, time-dependent manner (P < 0.05). AGE-HSA up-regulated the expression of FN mRAN and protein in dose- and time-dependently (P < 0.01); PKC activator phorbol 12-myristate 13-acetate (PMA) induced FN expression, respectively depletion of PKC and calphostin C, a PKC inhibitor, effectively prevented both PMA and AGE-HSA-induced expression of the FN (P < 0.05).

CONCLUSIONAGEs can increase the activities of PKC. AGEs can directly increase FN expression in HPMC which may contribute to peritoneal fibrosis and this is regulated by PKC.

Cells, Cultured ; Epithelium ; secretion ; Fibronectins ; metabolism ; Glycation End Products, Advanced ; pharmacology ; Humans ; Peritoneum ; cytology ; Protein Kinase C ; metabolism

OBJECTIVETo investigate the changes of the expression of intercellular adhesion molecule-1 (ICAM-1) and integrin beta(1) in peritoneal mesothelial cells during laparoscopy-assisted radical gastrectomy(LARG) and to explore the possible effects of LARG on the peritoneal metastasis.

METHODSFrom April to August 2008, LARG was performed for 26 patients with gastric cancer (laparoscopy group), while 20 cases underwent open radical gastrectomy(open group). Peritoneum of right upper belly was collected at 3 operation time points(the beginning, 2 hours, 4 hours). The expressions of ICAM-1 and integrin beta(1) in peritoneal mesothelial cells at 3 time points were detected by immunohistochemistry.

RESULTSWith the operation prolonging, the expression of ICAM-1 and integrin beta(1) was increased gradually in both LARG and open groups. The expression of integrin beta(1) in two groups was obviously increased at 4-hour time point as compared to the beginning(P<0.05). Besides, there were no significant differences of these two adhesion molecules among the three operation time points between two groups(P>0.05).

CONCLUSIONSCompared with open surgery, LARG is not associated with a greater effect on the expression of ICAM-1 and integrin beta(1) in peritoneal mesothelial cells, and may not promote peritoneal metastasis of gastric cancer through increasing the expression of adhesion molecule in peritoneal mesothelial cells.

Adult ; Aged ; Epithelial Cells ; metabolism ; Female ; Gastrectomy ; methods ; Humans ; Integrin beta1 ; metabolism ; Intercellular Adhesion Molecule-1 ; metabolism ; Laparoscopy ; Male ; Middle Aged ; Peritoneum ; cytology ; Stomach Neoplasms ; metabolism ; pathology ; surgery

OBJECTIVE: To determine the effect of indomethacin on high concentration glucose and lipopolysaccharide (LPS) induced fibronectin (FN) and plasminogen activator inhibitor-1 (PAI-1) secretion in cultured human peritoneal mesothelial cells. METHODS: Mesothelial cells were isolated from human omental specimens by trypsin disaggregation and incubated by 2.5% glucose or LPS together with different concentrations of indomethacin. Enzyme-linked immunosorbent assay determined the quantity of FN and PAI-1 in the cultured supernatants. RESULTS: Compared with the control group, the levels of FN and PAI-1 in the cultured supernatants were increased significantly exposuring to high concentration glucose and LPS (P <0.01). The different concentrations of indomethacin decreased FN and PAI-1 secretion in the 2.5% glucose or the LPS group within 24 h (P < 0.05). CONCLUSION Indomethacin can inhibit the synthesis and secretion of extracellular matrix in human peritoneal mesothelial cells, which may be effective in the gene therapy for peritoneal fibrosis.
Cells, Cultured ; Cyclooxygenase Inhibitors ; pharmacology ; Epithelial Cells ; metabolism ; Fibronectins ; metabolism ; Humans ; Indomethacin ; pharmacology ; Peritoneum ; cytology ; metabolism ; Plasminogen Activator Inhibitor 1 ; metabolism

In order to investigate the effect of ligustrazine (Lig) i.p. on peritoneal permeability in peritoneal dialysis and its side effects, creatinine was given intravenously and continuously to maintain the high plasma creatinine level. All the rabbits were divided into three groups: normal control group (group A), group B treated with 0.12% Lig and group C treated with 0.24% Lig. The peritoneal dialysis of all rabbits lasted 2 h. The plasma and dialysate levels of glucose, protein and creatinine were observed immediate, 30 min, 60 min, 90 min, 120 min after dialysis. Creastinine dialysate/plasma ratio (D/P), protein D/P ratio, glucose D/Do at different time points after dialysis and creatinine mass transfer area coefficient (MTAC) at 120 min were calculated. The structures of peritoneum were observed under optical microscope and electron microscope after continuously intraperitoneal injection of Lig for 14 days. The results showed that the 90-min and 120-min creatinine D/P ratios in the group C were higher than in the group A. The 120-min creatinine MATC in the group C was higher than in the group A. The rabbits treated with Lig did not show significant structure changes of peritoneum and signs of peritoneal irritation. It was suggested that Lig could increase mass transfer ability of peritoneum without significant side effects.
Animals ; Biological Transport ; Cell Membrane Permeability ; Creatinine ; blood ; Dialysis Solutions ; chemistry ; Peritoneal Dialysis ; methods ; Peritoneum ; metabolism ; Pyrazines ; pharmacokinetics ; pharmacology ; Rabbits

BACKGROUND: It used to induce hypercarbia that carbon dioxide insufflated into the peritoneum in laparoscopic surgery. It might stimulate sympathetic nervous system, and decrease splanchnic circulation, hepatic function, and metabolism of anesthetics. The purpose of the present study was to examine the influence of hypercarbia on concentrations of propofol at the time of eye opening and recovery of orientation after propofol target controlled infusion (TCI) during a laparoscopic cholecystectomy. METHODS: Fifty patients were divided randomly into a laparoscopic group (group 1, n = 25) and an exploratory group (group 2, n = 25). A propofol infusion was started at a propofol target concentration of 6microgram/ml, and anesthesia was maintained at 4microgram/ml by using a Diprifusor (TM) turing the operation, intraabdominal pressure was maintained automatically at 12 14 mmHg by a CO2 insufflator and controlled ventilation settings were adjusted about 50 mmHg of PaCO2 after peritoneal insufflation. This ventilatory setting was not changed throughout the operation. We evaluated the estimated plasma concentrations of propofol at the time of eye opening and recovery of orientation in each group using user interface of a Diprifusor (TM). RESULTS: In the laparoscopic group, PaCO2, and PetCO2 increased significantly at 5, 15, 30 minutes after carbon dioxide insufflation, but there was no significant difference in concentrations of propofol at eye opening and orientation after propofol TCI between the two groups. CONCLUSIONS: Hypercarbia induced by insufflation of carbon dioxide into peritoneum didn't give rise to an influence on awakening concentrations after propofol TCI during a laparoscopic cholecystectomy.
Anesthesia ; Anesthetics* ; Carbon Dioxide ; Cholecystectomy, Laparoscopic* ; Humans ; Insufflation ; Laparoscopy ; Metabolism ; Peritoneum ; Plasma ; Propofol* ; Splanchnic Circulation ; Sympathetic Nervous System ; Ventilation

In order to investigate the effect of ligustrazine (Lig) i.p. on peritoneal permeability in peritoneal dialysis and its side effects, creatinine was given intravenously and continuously to maintain the high plasma creatinine level. All the rabbits were divided into three groups: normal control group (group A), group B treated with 0.12% Lig and group C treated with 0.24% Lig. The peritoneal dialysis of all rabbits lasted 2 h. The plasma and dialysate levels of glucose, protein and creatinine were observed immediate, 30 min, 60 min, 90 min, 120 min after dialysis. Creastinine dialysate/plasma ratio (D/P), protein D/P ratio, glucose D/Do at different time points after dialysis and creatinine mass transfer area coefficient (MTAC) at 120 min were calculated. The structures of peritoneum were observed under optical microscope and electron microscope after continuously intraperitoneal injection of Lig for 14 days. The results showed that the 90-min and 120-min creatinine D/P ratios in the group C were higher than in the group A. The 120-min creatinine MATC in the group C was higher than in the group A. The rabbits treated with Lig did not show significant structure changes of peritoneum and signs of peritoneal irritation. It was suggested that Lig could increase mass transfer ability of peritoneum without significant side effects.
Biological Transport ; Cell Membrane Permeability ; Creatinine/blood ; Dialysis Solutions/chemistry ; Peritoneal Dialysis/*methods ; Peritoneum/*metabolism ; Pyrazines/*pharmacokinetics ; Pyrazines/pharmacology

No abstract available.
Antigens, CD/metabolism ; Cell Adhesion Molecules/metabolism ; Female ; Humans ; Immunohistochemistry ; Peritoneum/metabolism/pathology ; Sarcoma, Ewing/*diagnosis/pathology/radiography ; Tomography, X-Ray Computed ; Young Adult