Objective To understand the differences of virulence genes and molecular typing in Campylobacterjejuni isolates from poultry products and diarrhea patients in Shenzhen.Methods According to specific primers,four virulence genes (cdtB,cadF,flaA,virB1 1 )of C.jejuni were detected by polymerase chain reaction (PCR).Molecular typing for C.jejuni strains was performed by Pulsed-field gel electrophoresis (PFGE).Results There were no differences of gene distribution (cdtB+,cadF+,virB1 1-)between isolates from poultry products and diarrhea patients.Two virulence genes of cdtB and cadF were found in all of ten C.jejuni strains lacking virB1 1 .The carriage rate of flaA in food-borne isolates (3/5 )was higher than those in patient isolates (2/5).In the PFGE map,the clustering analysis of C.jejuni strains showed that a total of 5 to 9 DNA bands were observed in ten strains through the digestion of Sam I.There was high homology (above 85%) between food-borne isolates and patients isolates,but the distribution of flaA in these highly homologous strains was differ-ent.Conclusion So far,C.jejuni strains with cdtB,cadF and flaA were present in Shenzhen,and showed high diversity and homology.This implies that the occurrence of diarrhea in patients with C.jejuni was associated with the contaminated poul-try products by this pathogen.Their findings can provide basic data and evidences about diarrheal disease caused by food-borne C.jejuni for the local region.

Objective To investigate the effects of salvianolate lyophilized injection(SLI) on neural functional recovery and the expression of microtubule associated protein-2(MAP2) after focal cerebral ischemia-reperfusion injury in the diabetic rats.Methods Diabetes model was made by intraperitoneal injection of streptozotocin (STZ) and cerebral ischemia-reperfusion model was developed by Longa suture occluded method in the middle cerebral artery of diabetic rats.The rats were randomly divided into five groups: sham operation group, model group, SLI (21.0 mg.kg-1,10.5 mg.kg-1) treatment groups, and edaravone (6 mg.kg-1) treatment group.3 hours after ischemia,rats were respectively given normal saline or drugs followed by the injection once a day for 14 days and the neurological impairment was assessed.2 h after the last injection,the rats were decapitated and the brains were collected.The expression of MAP2 protein and mRNA in the bilateral hippocampal ischemia and infarcted area was detected with immunohistochemistry and RT-PCR.Results Severe neurological dysfunction was found in diabetic rats that had been subjected to cerebral ischemic injury (1.850±0.457).A significant improvement on neurological function was found in the SLI treatment groups (1.581 ± 0.314, 1.345 ± 0.425) compared with model group(P＜0.01, P＜0.05).Moreover,the expression of MAP2 in ischemia bilateral hippocampal CA1 and penumbra was represented by the average optical density value respectively (0.743±0.250,0.561± 0.224).In the hippocampal CA1 region, the number of MAP2-positive cells (0.781 ± 0.420 , 0.851 ± 0.136) in the treatment group showed significant increase than those in model group (P＜0.01, P＜0.05).In the ischemic penumbra region,the number of MAP2-positive cells (0.753±0.235,1.203±0.326) in the treatment group showed significant increase than those in model group (P＜0.01, P＜0.05).Conclusion The SLI can promote the post-injury neurocognitive function in diabetic rats.The increase of MAP2 expression may be involved in the mechanisms.

Objective: To understand the quality of life and its influencing factors of adolescent from low-income families in Nanning City. Methods: With the help of the community, junior and senior high school students from 1 144 low-income households and 1 067 non-minimum households in 4 districts of Nanning City were randomly selected to complete a questionnaire survey regarding quality of life and study stress source. Results: The quality of life and total scores of adolescents in the low-income families were significantly lower than the national norm(P<0.01), and also lower than the scores of adolescents from normal in come families. The scores of boys in low-income households were significantly higher than those of girls in terms of exercise quality, life chance, negative emotions, and other factors (P<0.05). Girls scored significantly higher in teacher-student relationships, parent-child relationships, and self-concepts than boys (P<0.05). Junior middle school students were significantly higher than high school students in terms of convenience, athletic ability, teacher-student relationship, physical feelings, work attitude, life opportunity, negative emotions, peer relationships, self-satisfaction, other and total scores.Differences were of statistical significance (P<0.05). Regression analysis showed that there are 9 factors that affect social and psychological functions; 8 factors that affect living environment; 5 factors that affect physiological and mental health; 5 factors that affect satisfaction with quality of life; and 4 factors that affect total quality of life(P<0.05). Conclusion The quality of life of middle school students from low-income families is low, and effective measures should be developed and implemented accordingly.

Objective: To understand the status of suicide and self-injury among college students of Yao nationality in Guangxi and their relationship with psychological adjustment ability, and to support the prevention and intervention of suicide and self-injury behavior of Yao university students. Methods: From April to May 2019,a questionnaire survey was conducted among 844 Yao university students in three universities in Guangxi in demographics, psychological resilience, and forgiveness. Results: Among the students of the Yao ethnic group in Guangxi, 10.90% had suicidal ideation; 4.62% had suicide plan; 3.55% had suicide preparation; 2.84% had suicidal action; and 12.56% had suicidal behavior. The total scores of students with suicidal ideation in Psychological Resilience, Self-Compassion and Hearland Forgiveness Scale were significantly lower than those without suicidal ideation, which was statistically significant(t=-3.74, -5.54, -4.97,P<0.01). Multivariate Logistic regression analysis showed that a high score for forgiveness was negatively correlated with suicidal behavior(OR=0.28) and self-harm behavior (OR=0.32)(P<0.05). Conclusion The psychological resilience, forgiveness, and self-compassion of Yao college students are closely related to suicide-related psychological behaviors and self-harm behaviors. Suicide and self-harm behaviors can be reduced by improving the self-psychological adjustment ability of Yao college students.

To establish a method for the determination of formaldehyde and glyoxal simultaneously in varenicline tartrate active pharmaceutical ingredient (API) and its intermediate, formaldehyde and glyoxal were derivatized by 2, 4-dinitrophenylhydrazine (2,4-DNPH) to improve the HPLC retention and UV detection sensitivity. Separation was performed on a C8 (150 mm × 4.6 mm, 5 μm) column by linear gradient elution using acetonitrile and water as the mobile phase; the detective wavelength was set at 380 nm.Formaldehyde and glyoxal were quantitatively determined by an external reference method.Linear calibration was established for both formaldehyde and glyoxal in the range from 0.094 to 1.88 μg/mL.The detection and the quantification limits were 0.047 μg/mL (19 μg/g) and 0.094 μg/mL (38 μg/g), respectively.The recoveries were (95.0±1.1)% and (99.4 ± 2.6)% for formaldehyde and glyoxal, respectively.This method has been fully validated to be applicable to quantitative analysis of trace amount of formaldehyde and glyoxal in varenicline tartrate API and its intermediate.Test results demonstrated that the contents of both formaldehyde and glyoxal met the permitted daily exposure (PDE) limits for the finished products of varenicline tartrate API as well as its intermediate, though the glyoxal contents in the crude intermediates were likely to exceed the limit.The established method is valuable for the manufacturing process and quality control of varenicline tartrate.

This study aimed to identify the related substances of lorazepam tablets by liquid chromatography mass spectrometry (LC-MS). To separate the related substances of lorazepam tablets, gradient elution was performed using acetonitrile and 0.1% acetic acid -20 mmol/L of ammonium acetate as mobile phase on Inert Sustain C18 (250 mm × 4.6 mm, 5 μm).The accurate mass and elemental composition of the parent ions and their product ions of related substances were determined by electrospray-ionization quadrupole time-of-flight high resolution mass spectrometry (ESI-Q-TOF/MS).The structures of the related substances were identified by spectral analysis. Under the established analytical condition, lorazepam and its related substances were adequately separated, and 22 major related substances with content greater than 0.1% were detected and identified by hyphenated techniques in lorazepam tablets and their stressed samples.Among them, 5 were the impurities listed in the USP or EP, and the others were unknown related substances identified for the first time in this paper.The LC-MS technique can effectively separate and identify the related substances of lorazepam tablets, which provides some reference for quality control.

Objective:To assess the change and inter-individual variation in body temperature and analyze related variables among Chinese adults.Methods:Data of of 9 184 participants[mean age(52.38±14.69) years, 4 350 men and 4 834 women] were obtained from the China Health and Nutrition Survey(CHNS) 2015. Descriptive statistics and liner regression models were deployed in the analysis.Results:Overall, the mean normal axillary body temperature was 36.43℃, higher temperature was observed in men(0.032℃, P<0.001) than women. Using the multivariate linear regression model, it was revealed that age, region, territory, season, height, and hip circumference were significantly associated with body temperature. Higher individual temperature was observed in urban(0.028℃) than rural, in southern(0.040℃) than northern, and in winter(0.054℃) than autumn. Body temperature was also negatively associated with age(-0.001℃ per year), hip circumference(-0.001℃ per cm), but is positively associated with height(0.003℃ per cm). With other variables controlled, body temperature was related to whether there is myocardial infarction(ever vs never 0.187℃). Conclusion:In China, the current normal body temperature is lower than that established in 19th century(37℃), and body temperature is related to several factors. When screening and diagnosing a disease, inter-individual variance should be fully considered.