0 05). Conclusion Femal, splenomegaly and increase of the MPV width and PVP were the risk factors inducing PVT in liver cirrhosis patients, while liver function, BPC, PT, ect, may not be related to the formation of PVT.

Objective To clarify the relationship between ABO blood group and the development of duodenal ulcer(DU)in aspects of gastric acia and Hp infection.Methods The blood group of ABO was determined in 80 patients who were diagnosed as DU and received 24-hour gastric pH monitoring between 1995 and 2003.These results were compared with the expected frequency in the 1061 healthy controls in Beijing.The prevalence Helicobacter pylori (Hp) infection rate was determined by rapid urinase test,biopsy and 13C breath test.Results Blood type O was present in 56.3% of the patients with DU,which was significantly higher than the expected rate (28.7%) in healthy population (? 2=26.69,P0.05).Conclusion Blood group O doesn’t cause the disease by affecting Hp infection rate or stronger gastric acid secretion,it maybe another independent risk factor for DU.The onset age in blood group O is not different from that in other types.The mechanism needs to be explored.

Objective To investigate the effect of the formation of portal vein thrombosis(PVT) on the nature course of liver cirrhosis(LC). Methods Patients with LC during 1995 2002 in our hospital were reviewed and forty eight cases of LC with PVT were included in the study. The PVT was diagnosed by color Doppler and/or CT. Fifty two cases of LC patients without PVT were chosen as controls. The liver function, coagulation function and the aspects of portal hypertension etc. were compared between these two groups. The average diameter of main portal vein(MPV) and spleen vein (SPV) were measured by color ultrasound. Results In 75.0% of patients, thrombosis happened gradually without symptoms, and 85.4% happened in the MPV trunks. The splenomegaly and width of MPV were the risk factors for the formation of PVT ( P = 0.003 and 0.010). In PVT group, the average width of MPV and SPV was 1.48 cm ?0.26 cm and 1.23 cm ?0.38 cm , respectively, significantly wider than 1.37 cm?0.22 cm and 1.05 cm ?0.30 cm in controls( P =0.037 and 0.031). In addition, larger size of spleen,more severe esophageal varices, higher rate of severe variceal haemorrhage and larger volume of ascites after portal vein thrombosis were shown in the study group( P

Objective To prepare recombinant human epidermal growth factor (rhEGF) sustained-release microspheres and evaluate their morphology, rhEGF releasing activities and cell proliferation activity in vitro and compare difference of rhEGF sustained-release microspheres and rhEGF in facilitaring ulcer healing in diabetic rats. Methods (1) rhEGF sustained-release microspheres were prepared by the modified double emulsion method. Morphology of the microspheres was detected by transmission electron microscope and size distribution measured by laser granularity meter/Zeta electric potential meter. ELISA assays were applied to determine rhEGF releasing. (2)Proliferation of mouse fibroblasts was analyzed by MTr method. (3) Diabetic rat models were prepared and divided into four groups, ie, rhEGF sustained-release mierospheres group (Group A), rhEGF stock solution group (Group B), blank sustainedrelease mierospheres group (Group C) and PBS meustruum control group (Group D), which were given drug once a day. The wound healing rate was calculated by taking photographs at days 3,7,14 and 21. Skin specimens from the wound edge were harvested partially for observation of hydroxyproline (HYP) contents. Immunohistochemistry was employed to detect integrin 131 and keratin-19 and measure their positive staining area ratio. Results (1) The particle diameter of rhEGF sustained-release microspheres was 193.5 nm, with relative uniform particle diameter distribution. There showed no conglutination among rhEGF susrained-release microspheres, with good dispersibility. Releasing drug lasted for 24 hours and accorded with Higuchi release kinetic model. (2) Different concentrations of rhEGF sustained-release microspheres could promote the proliferation of mouse fibroblast, especially the concentration of 10 μg/L (P <0.05, compared with the control). (3) From the 7th day after treatment, Group A had the fastest wound healing rate, with statistical difference compared with other three groups (P < 0.05). Group A had higher HYP contents and positive area ratio of integrin β1 and keratin-19 than Group B. Conclusions rhEGF sustained-release microspheres prepared by the modified double emulsion method have uniform particle size and can last release for 24 hours. Compared with rhEGF stock solution, rhEGF sustained-release microspheres have faster and better ulcer healing and higher healing quality in diabetic rats.