The paper is aimed to study the enzymatic hydrolysis of the activatable cell-penetrating peptide (ACPP) that was designed and synthesized. The ACPP was composed of three parts, polyanionic sequence peptide, peptide sequence that specifically cleaved by matrix metalloproteinase (MMP) and cell penetrating peptide (CPP). The ACPP was hydrolyzed by type IV collagenase (MMP-2/9) under the condition of 37 degrees C and was monitored by reversed-phase high performance liquid chromatography (RP-HPLC). The efflux of peak was collected and detected by matrix assisted laser desorption ionization orthogonal time of flight mass spectrometry (MALDIO-TOF-MS) to speculate the sequences of the peptide fragments. The results indicated that the ACPP could be cleaved by type IV collagenase at target site as predicted, released CPP. The half life of the cleavage was about 4 h. Meanwhile, the peptide fragments may be cleaved again at other sites by type IV collagenase.

Objective To observe the biomechanics of the anterior and posterior interosseous talo-calcaneal ligaments (ITCLs) . Methods Load-displacement characteristics of the subtalar joint were studied in 12 cadaver specimens whose ankle joints were mutilated. The ankle articular surfaces of the talus and the post-calcaneus were exposed. Bone blocks were then embedded in polymethylmethacrylate. The an-terior ITCLs were abscised in 6 of them, and the posterior ITCLs in the other 6. A multi-functional biome-chanical machine was used to perform the biomechanical tests on the specimens. Results When the anterior ITCLs were cut, the tali moved to the anterior-lateral side. When the posterior ITCLs were cut, the tali mostly moved to the anterior-interior side. Conclusions The anterior and posterior ITCLs have dif-ferent roles in maintaining stability of the subtalar joint. Since the posterior ITCLs seem more important than the anterior ones, they should have priority in ITCL reconstruction.

Digital X-ray photography has been applied to clinical medicine in recent 3years.This paper introduces the development situation of the flat detectors for digital X-ray photography.The technical indexes and structure differences of the detectors are presented in this paper.

Objective: Introduce Internet and wireless communication technology into radiology image remote diagnosis system, develop a new system combined with PACS. Material and Methods: Used ASP.NET technology under VS. NET2005, and used JAVA to develop the image APPLET, Web server used 11S5.0, database used Microsoft SQL Server. Also need a SMS Modern to send message online. Results: Offer two ways to access medical image remote diagnosis system: Interact and wireless networks. Wherever a doctor he is, as long as has a computer or mobile phones can be connected to Interact or wireless network, can login diagnosis system, browse the patients' images, do some image processing, and also can send reports. Conclusions: The system can be put into practice, lifting the restrictions in time and place of remote diagnosis system, it try in full-development of telemedicine. Although at present reports of this form does not have legal effectiveness, this paper provided a new idea for development of medical image remote diagnosis system.

Objective To design a new X-ray sensor of iono-chamber type to achieve automatic exposure control(AEC) of the X-ray machine. Methods We designed the chamber acording to the ionization theory and the requirements of automatic exposure control. Results (1) The device had double-negative poles, so the distance between positive and negative poles was 1/2 shorter and its test-volume was equal to odd-negative pole. (2)It had good property of electromagnetism-resistance because of the double shielded design by double-negative poles and screen-rings.(3) Iono-chamber was made of low-density material, so it had low absorb-ratio of X-ray and could not form image on film. Conclusion The property of the device was steady and reliable. It had applying and spreading value.

Objective To evaluate the clinical efficacy of external biplanar mini-fixator and limited internal fixation in treatment of severe midfoot fracture-dislocation.Methods Eight patients with severe midfoot fracture-dislocation were admitted from May 2009 to August 2012.They were 6 men and 2 women,28 to 57 years of age (average,35.4 years).According to the Gustilo classification,there were one type Ⅱand 7 cases of type Ⅲ.Two cases of midfoot fracture were complicated with dislocation of the transverse tarsal joint,4 with dislocation of the tarsometatarsal joint,and 2 with dislocation of both the transverse tarsal joint and the tarsometatarsal joint.The external biplanar mini-fixator was applied to stabilize and maintain lengths of the medial and lateral columns and normal geometry of the foot arch after reduction of the fracture-dislocation.K-wires were used if limited internal fixation was necessary.The external fixator was removed at 12 to 16 weeks postoperatively.The clinical treatment efficacy was assessed by the American Orthopaedic Foot and Ankle Society (AOFAS) midfoot scale and the visual analogue scale (VAS).Results The patients were followed up for an average of 13.4 months (from 10 to 24 months).Postoperative radiological films showed fine reduction of the fractures and dislocations.No reduction was lost after removal of external and internal fixations.They were able to walk independently and had good functional recovery of the injured feet without traumatic arthritis during the follow-up period.Their average AOFAS scores were 65.8 at 3 months after operation,71.3 at 6 months and 80.6 at 12 months; the average VAS scores were 7.5 at 3 months,4.3 at 6 months,and 1.2 at 12 months,respectively.Conclusion In treatment of severe midfoot fracture-dislocation,external biplanar mini-fixator combined with limited internal fixation can perfectly reconstruct and maintain the medial and lateral columns and normal geometry of the foot arch,preventing functional disability caused by foot malformation.

Cyclic amp receptor protein (CRP) is a global transcriptional factor in many prokaryotes, capable of governing nearly half of the total genes in Escherichia coli. Through the method of error-prone PCR or DNA shuffling, we can first obtain CRP mutant library and then get the expected cell phenotype with enhanced resistance. In this article, we reviewed the following desired phenotype: enhanced tolerance towards oxidative stress, improved osmotolerance, enhanced organic solvent (toluene) tolerance, improved acetate tolerance of E. coli fermentation and improved ethanol tolerance during bio-ethanol production. We then concluded that CRP can also be applied in other host cells to get desired phenotypes. Last, we predicted potential applications of mutant CRP transcriptional factor.
Cyclic AMP Receptor Protein ; biosynthesis ; DNA Shuffling ; Escherichia coli ; metabolism ; Fermentation ; Metabolic Engineering

Objective]To introduce Professor Lian’s experience of recuperating tumor with Chinese medicine. [Method]To analyze the col ection of Professor Lian’s clinical experience. [Result]Based on pulse and syndrome, using famous descriptions,which is the key of the effectiveness. [Conclusion] Professor Lian is ful of clinical experience, whose descriptions are common and worth studying.

Objective In order to investigate the effectiveness of unique cyclic biomechanical stretch on proliferation, alkaline phosphatase (ALP) activity and osteopontin (OPN) mRNA of colonal murine osteoblast-like cell line MC3T3-E1 in three-demensional culture. Methods Gelatin sponges with a size of 2.00 cm?2.00 cm?0.25 cm were used as the three-demensional culture vector of MC3T3-E1, 100 ?l cell suspension was applied to the surface of sponge, the cell number was 1.25?105. The gelatin sponges could be stretched to 5% with 60 cycles/min and 15 min/h on computerized bio-stretch controller. At 2, 4, 6, 8 and 10 d after stretch stimulation, three samples were collected at each time point from the stretch and con-trol group respectively. Cell counting, activity assay of ALP both of cells and medium as well as OPN mRNA measurement were carried out. Results The cell number of the stretch group was more than that of control group from 2 d (P

Objective To observe the clinical results of free dorsal forearm mini-osteo-cutaneous flap to repair the bone and soft tissue defect of finger. Methods The mini-osteo-cutaneous flap pedicled on the osteo-cutaneous branch of dorsal branch of anterior interosseous artery was designed on the radial side of the distal dorsal forearm along the connective line between the Lister's tubercle and the lateral epicondyle of the humerus, the point of the cutaneous branch of dorsal branch of anterior interosseous artery entry the skin was about 3.5 cm proximal to the Lister's tubercle. The dimensions of the osteo-cutaneous-flap were planned according to the defect of recipient. The incision begins along the ulnar board of the flap and the septum between the extensor pollicis brevis muscle and the extensor pollicis longus muscle was exposed, the osteo-cutaneous branch of dorsal branch of anterior interosseous artery was indentified, then the cutaneous flap and osteo-flap was raised respectively, the osteo-cutaneous branch was dissected deeply to the origin and sectioned, the donor site was closed directly. The vascular pedicle of the osteo-cutaneous flap was anastomosed with the digital artery and dorsal vein respectively. From Jul. 1999 to Jan. 2005, 7 fingers of 7 males suffered bone and soft tissue defect with the average of 25 years old ranging from 18 to 37 years old were performed. Results All the 7 free mini-osteo-cutaneous flaps were survived completely, the dimensions of the cutaneous-flap and osteo-flap were 2.5 cm ? 3.0 cm to 4.5 cm ? 3.5 cm, 2.5 cm ? 1.0 cm to 3.5 cm ? 1.2 cm respectively. The osteo-flap was united with recipient bone 2 to 3 months after the transplantation and the donor site was closed by direct suture without tension, the appearance of the reconstructed finger by mini-osteo-cutaneous flap was perfect. Conclusion The free dorsal forearm mini-osteo-cutaneous flap pedicled on the osteo-cutaneous branch of dorsal branch of anterior interosseous artery was one of the optimal flap to reconstruct bone and soft tissue defect of the finger.