Hospital infection has become a global public health problem. The strain of anti-disinfectant, same as the antibiotic-resistant strain, will lead to the failure of the disinfection of the hospital, which will cause hospital infection. The bacterial resistance to disinfectants and resistance mechanism is not clear. Most of the studies focused on the cell research, the molecular research, non-physiological mechanism of resistance, in recent years, the selection pressure, outflow pump were studied. Currently, disinfectants abuse, improper handling and inadequate amount of disinfectant were considered as the main cause of the resistance development.

Objective To investigate and analyze nasal carriage of bacteria in medical staff and antibiotic susceptibility. Methods All study participants underwent swabbing of the anterior 1.5 cm of the nasal vestibule of both nares with a sterile swab,and did drug sensitivity test Apr.-Oct.,2007. Results In 41.08% of all samples (125/299),136 pathogenetic bacteria were isolated. Klebsiella pneumoniae,Staphylococcus aureus and Enterobacter aerogenes were the prominent bacteria,of which showed the resistance for more than three kinds of antibiotics were 35,accounting for 25.73% of all isolates,10 strains of Methicillin-resistant Staphylococcus (MRS) were detected. Conclusion The nasal carriage of bacteria and antibiotics resistance in medical staff in Tianjin should be paid attention to,for it may an important factor in the control of infection in hospitals.

Objective To observe the efficacy of cytokine induced killer (CIK) cells combined with radiofrequency ablmion (RFA) in treatment of liver metastases.Methods 23 patients were treated with CIK plus RFA, while 27 patients with RFA.Tumot markers and CD3+、CD4+、CD8+、CD4+/CD8+ were tested before and after treatment.Recurrence rate and Kamofsky score were also evaluated.Results The local recurrence rate in the study group was lower (13.04% vs.40.74%)(P<0.05),CD3+、CD4+、and CD4+/CD8+ were increased (P<0.01). The percentage of patients with decreased tumor markers and increased Kamofsky is higher (P<0.05);Conclusion The therapy of CIK combined with RFA has better efficacy on liver metastases,can enhance the cellular immune functions and improve the quality of life significantly.

OBJECTIVE To investigate and analyze hand carrier state of medical staff in Tianjin and the antibiotic resistance of the pathogens.METHODS Bacteria or pathogens were isolated from the samples using a sterilized cotton swab on the whole hand before hand washing or hand disinfection,and drug sensitivity test was performed.RESULTS In 16.72% of all samples(50/299),there were 64 kinds of bacteria or pathogens isolated.A.baumannii,Staphylococcus aureus and A.lwoffii were the most common bacteria,40.6% resistant to more then 3 kinds of drugs.Four Meticillin-resistant S.aureus(MRSA) strains were detected out.CONCLUSIONS The high rate of hand carrying bacteria and resistance in Tianjin should pay attention to.

Acupuncture Therapy ; Humans ; Huntington Disease ; physiopathology ; therapy ; Male ; Middle Aged ; Motor Activity

OBJECTIVETo probe into an effective method for treatment of poststroke depression.

METHODSTwo hundred and fifty-six cases of poststroke depression were randomly divided into a control group (n = 76) treated by oral administration of amitilin and an acupuncture group (n = 180) treated by activating brain and regaining consciousness needling method. Their clinical therapeutic effects and changes of relative factors of poststroke depression and plasma contents of monoamine neurotransmitters were observed.

RESULTSIn the acupuncture group, the clinical symptoms and the relative indexes in the depression scales improved and plasma contents of the monoamine neurotransmitters increased significanty as compared with the control group (P < 0.05).

CONCLUSIONThe needling method for activating brain and regaining consciousness can improve relative factors to cure poststroke depression.

Acupuncture Therapy ; Brain ; Consciousness ; Depression ; therapy ; Depressive Disorder ; Humans

OBJECTIVETo observe effects of acupuncture on depression and anxiety, and glucose metabolism in the patient of type 2 diabetes.

METHODSOne hundred cases of type 2 diabetes mellitus patients were randomly divided into the control group treated with oral administration of Diaformin and the observation group treated with oral administration of Diaformin combined with acupuncture at Baihui (GV 20), Fengfu (GV 16), 50 cases in each group. Before and after treatment, depression scale (HAMD, SDS) and anxiety scale (HAMA, SAS) scores, and fasting blood glucose (FPG), blood glucose 2 h after meal (PG 2 h), glycosylated hemoglobin (GHb) levels were detected.

RESULTSAfter treatment, the mood disorder improved and FPG, PG 2 h, GHb levels decreased in the two groups, with the observation group being better than the control group (P < 0.05).

CONCLUSIONAcupuncture can improve negative mental state, glucose metabolism and blood glucose in type 2 diabetes patients.

Acupuncture Therapy ; methods ; Adult ; Affect ; Blood Glucose ; analysis ; Diabetes Mellitus, Type 2 ; psychology ; therapy ; Female ; Glucose ; metabolism ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged

Objective To analyze the correlation of hypersensitive C-reactive protein(hs-CRP),homocysteine(Hcy) and D-dimer(D-D) with pathological change degree of coronary heart disease(CHD),and to investigate the diagnostic specificity and sensitivity of joint detection in CHD.Methods A total of 100 cases of CHD patients(experimental group) and 100 healthy subjects(control group) were enrolled,.Patients of the experimental group were divided into four groups on the basis of complications,including simple CHD group(32 cases),amalgamating hypertension group(46 cases),amalgamating diabetes group(9 cases),amalgamating hypertension diabetes group(13cases).The levels of hs-CRP,Hcy and D-D in the five groups were detected and analyzed.Results The levels of hs-CRP,Hcy and D-D in the experimental group were all higher than the control group(P<0.05).Logistic regression confirmed that three indexes were the independent risk factors for CHD.Each indicator has a certain clinical significance to the diagnosis and treatment of CHD but the value of Hcy could be better.Joint detection of hs-CRP and Hcy could be an ideal combination of detection,and the three joint detection might not be suitable for early diagnosis and treatment of CHD.The levels of hs-CRP and D-D in simple CHD group,amalgamating hypertension group,amalgamating hypertension diabetes group were all higher than amalgamating diabetes group(P<0.05).Conclusion hs-CRP,Hcy and D-D could be the independent risk factors of CHD,and joint detection might be with important clinical value for diagnosis of CHD.

Objective To survey the retraction of dental unit waterlines (DUWLs)in medical institutions,and evaluate the influencing factors for retraction of DUWLs.Methods In May-November 2014,dental chair units (DCUs)in medical institutions in Tianjin City were sampled through systematic random sampling method,retrac-tion volume of DUWLs was detected by independently made detector,the relevant influencing factors were investi-gated.Results A total of 58 DCUs in 30 medical institutions in 10 districts(counties)of Tianjin were investigated, the average retraction volume of DUWLs was (103.60 ±117.85 )mm3 ,the qualified rate of retraction volume of DUWLs was 48.28 %(28/58);length of utilization of DCUs was positively correlated with DUWLs retraction (r=0.52,P <0.001).There were no significant difference in retraction volume of DUWLs among medical institutions in different districts(counties),levels of medical institutions,DUC brands,and modes of DUC water supply(all P >0.05 ).Conclusion The qualified rate of retraction volume of DUWLs is low,routine maintenance of DUCs needs to be intensified,especially the long-term used DUCs,contaminatin of DUWLs due to the invalidity of retraction valve should be prevented.

Objective To study multiple cytokine mRNA expressions of bone marrow stromal cells in patients with multiple myeloma(MM),as well as to explore the role of these cytokines in the occurrence and development of MM.Methods Semi-quantitiative RT-PCR was used to detect the mRNA expressions of IL- 1?,IL-6,SCF,TPO.Results The mRNA expression levels of IL-1? and IL-6 were higher than that of nor- mal controls and other hematological malignancies(P