Objective To investigate the effect of Cynanoside H on the proliferation and metastasis of triple negative breast cancer(TNBC) MDA-MB-231 cells and its mechanism, so as to provide an experimental basis for the development of TNBC therapeutics.Methods MDA-MB-231 cells were randomly divided into one control group(without Cynanoside H) and three test groups including 2. 5, 5 and 10 μmol/L Cynanoside H dose groups. The effect of Cynanoside H on the proliferation of MDA-MB-231 cells was detected by MTT assay and clone formation assay, while the effect on the cell cycle was detected by flow cytometry, and the effect on the metastasis of MDA-MB-231 cells was measured by wound healing assay, Transwell migration and invasion assay. In addition, the effects of Cynanoside H on the expression of cell cycle(c-Myc, CDK1, CDK2and cyclin E1), metastasis(E-cadherin, Vimentin and β-catenin) and PDGFRB/JAK2/STAT3 pathway related proteins(PDGFRB, p-JAK2, JAK2, p-STAT3 and STAT3) in MDA-MB-231 cells were determined by Western blot.Results The levels of cell viability(t = 4. 598, 19. 77 and 53. 43, respectively, each P < 0. 05), growth(36 h: t = 8. 256, 11. 57 and12. 16, respectively, each P < 0. 05; 48 h: t =10. 49,22. 49 and 19. 63, respectively, each P < 0. 01; 72 h: t = 50. 20, 28. 84and 15. 83, respectively, each P < 0. 01; 96 h: t = 11. 18, 18. 35 and 20. 92, respectively, each P < 0. 01) and clone formation(t = 4. 618, 9. 821 and 12. 14, respectively, each P < 0. 05) of 2. 5, 5 and 10 μmol/L dose groups were significantly lower than those of the control group. Compared with the control group, the proportion of cells in S phase of three test group increased in a concentration-dependent manner(48 h: t = 6. 316, 8. 156 and 14. 11, respectively, each P < 0. 05; 72 h: t =7. 231, 15. 36 and 25. 16, respectively, each P < 0. 05), and the expression of c-Myc(5 and 10 μmol/L:t = 10. 39 and12. 18, P < 0. 05 and < 0. 01, respectively), CDK1(t = 3. 777, 5. 069 and 6. 974, respectively, each P < 0. 05), CDK2(5 and10 μmol/L:t = 12. 72 and 19. 43, respectively, each P < 0. 01) and cyclinE1(t = 3. 813 and 15. 23, respectively, each P <0. 01) was down-regulated at the protein level. Compared with the control group, each test group significantly inhibited wound healing(48 h: t = 6. 969, 56. 16 and 27. 73, respectively, each P < 0. 05; 72 h: t = 8. 619, 22. 12 and 32. 15, respectively, each P < 0. 05), migration(t = 9. 817, 14. 74 and 19. 39, respectively, each P < 0. 01) and invasion(t = 5. 614, 13. 85and 14. 22, respectively, each P < 0. 01) of MDA-MB-231 cells, and up-regulated the expression of E-cadherin(10 μmol/L:t = 11. 79, P < 0. 01), down-regulated the expression of Vimentin(5 and 10 μmol/L:t = 12. 05 and 13. 02, respectively,each P < 0. 01) and β-catenin(5 and 10 μmol/L:t = 4. 516 and 9. 305, respectively, each P < 0. 01) at the protein level. In addition, the test groups of Cynanoside H could significantly down-regulate the protein expression of PDGFRB(5 and10 μmol/L:t = 7. 083 and 18. 87, respectively, each P < 0. 01) and inhibit the protein levels of p-JAK2(t = 4. 050, 10. 95and 11. 05, respectively, each P < 0. 05) and p-STAT3(5 and 10 μmol/L:t = 15. 25 and 25. 89, respectively, each P <0. 01), but had no effect on the total protein levels of JAK2 and STAT3.Conclusion Cynanoside H inhibits the proliferation of breast cancer MDA-MB-231 cells by inducing S-phase cell cycle arrest and reverses the epithelial-mesenchymal transition(EMT) to inhibit cell metastasis. These effects may be mediated by down-regulating PDGFRB/JAK2/STAT3 signaling pathway.

Objective:To study the integrated information nursing mode of perioperative medical care under the multidisciplinary cooperation of hip fracture in the elderly patients, provide reference for perioperative nursing of elderly hip fracture.Methods:A total of 1 019 patients with hip fracture from May 2018 to May 2019 in Beijing Jishuitan Hospital were randomly selected as the study object, and the non synchronous control was used for the study. From May to December 2018, 527 elderly patients with hip fracture in orthopaedic department of general trauma were taken as the control group, adopting the traditional treatment and nursing mode; from January to May 2019, 492 elderly patients with hip fracture in elderly trauma orthopaedics department were taken as the intervention group. Under the multidisciplinary cooperation, the information-based nursing mode of integrated medical care was implemented, and a multidisciplinary integrated medical care team was established. The information-based platform was used to optimize the clinical nursing path, and standardized, individualized and continuous nursing scheme was developed. The time from visit to hospitalization, the time from hospitalization to operation, hospitalization time, incidence of more than moderate pain, incidence of venous thromboembolism, incidence of stress injury, incidence of delirium and satisfaction of patients and their families at discharge were compared between the two groups.Results:The number of patients in the intervention group was higher than that in the control group within 4 and 24 hours. The rate of operation in 48 h increased from 8.0%(42/527) to 50.0%(246/492), and the rate of operation in 1 week increased from 70.0%(369/527) to 99.0%(487/492). Compared with the control group, the incidence of more than moderate pain in the intervention group decreased from 85.0%(448/527) to 2.0%(10/492), the incidence of venous thromboembolism in the hospital decreased from 12.0%(63/527) to 4.1%(20/492), and the incidence of pressure injury in hip fracture in the elderly decreased from 2.1%(11/527) to 0.2%(1/492); the incidence of delirium in the hospital decreased from 21.1%(111/527) to 8.1%(40/492); the hospital stay was shortened from (13.42 ± 3.36) d to (7.26 ± 2.03) d, and the satisfaction of patients and their families increased from 60.9%(321/527) to 95.9%(472/492). There were statistical significance between the two groups (χ 2 values were 4.157-708.006, t value was -35.119, P<0.05 or 0.01). Conclusions:The information-based nursing mode of integrated medical care under the multidisciplinary cooperation can adapt to the development of hip fracture in the elderly, which is conducive to the accelerated rehabilitation of patients.

Objective:To evaluate the effects of different nutritional supports in gastric cancer patients undergoing radical resection.Methods:Sixty patients with gastric cancer were randomly divided into three groups: parenteral nutrition(PN)group,enteral nutrition(EN) group and parenteral nutrition combined with enteral nutrition(PN-EN) group.The rate of complication, recovery time of the gut function,average post-operative hospital stay,changes of liver function,levels of blood glucose,serum albumin,prealbumin and transferrin,levels of nitrogen balance and change of body weight were observed in the three groups.The therapeutic effects of post-operational nutritional support were studied.Results:There are no significant difference in the rate of complication,average post-operative hospital stay,changes of liver function and level of blood glucose.The recovery time of the gut function in EN group was shorter than that in PN and PN-EN groups(P

Objective To analyse the effect of hedyotisdiffusa on expressions of apoptosis related protein Fas , caspase3 and caspase7 in Renca renal cell carcinoma of model mice.Methods One hundred and twenty BALB/C male mice were randomly divided into normal control group, model control group, interleukin group, hedyotisdiffusa group, 30 mice in each group, half male and female.Excepted for normal control group , renal cell carcinoma models were established in the other groups , and were given corresponding drug treatment.The tumor size and weight , and the tumor inhibition rate of all mice were observed, and the expression levels of Fas,FasL,caspase3 and caspase7 were detected.Results Compared with model control group, the tumor size and weight were lower , the tumor inhibition rate were higher , the positive expression rates and protein levels of Fas were higher , the positive expression rates and protein levels of FasL were lower, the positive expression rates and expression levels of Caspase3,Caspase7 protein were higher in the mice of interleukin group and hedyotisdiffusa group, all with significant differences ( P<0.05 ).The above indicators in hedyotisdiffusa group were significant improved compared with interleukin group (P<0.05).Conclusion The hedyotisdiffusa can effectively promote the expressions of apoptosis related protein Fas,caspase3 and caspase7 in renal cell carcinoma of model mice, inhibit the expression of FasL protein, improve the tumor suppression rate.

Objective To develop a kind of system of simulation and training on ECG for studying ,training and assessing of biomedical engineer. Methods The simulation training system was designed based on PC. In the hardware system, ADuC848 SCM was adopted as the primary controller, QuickStart exploration platform was used to develop firmware, and intelligent assessing system was explored by C++ Builder. Results Biomedical Engineer could be trained, checked and evaluated by the system. Conclusion The system, combining practice and examination, pays attention to basic skill training and thus improves studying efficiency.

Objective To investigate the influence of early postoperative enteral nutrition on the immunologic function and inci-sion infection in the patients with colorectal cancer .Methods 81 postoperative cases of colorectal cancer were randomly divided into the early parenteral nutrition(41 cases) ,and the early enteral nutrition(40 cases) by the random number table method .The anal ex-haust time ,ambulation time and occurrence situation of incision infection ,etc .were compared between the two groups ,and the im-munological indexes changes were also compared .Results Postoperative anal exhaust time ,bed activity time and the occurrence rate of incision infection in the early enteral nutrition group were significantly lower than those in the early parenteral nutrition group (P<0 .05);Th1/Th2 and lymphocyte count in the early enteral nutrition group were significantly higher than those in the early parenteral nutrition group(P<0 .05) .Conclusion The early postoperative enteral nutrition support in the patients with colorectal cancer can effectively improve the body's immune function and is an ideal method of nutritional support .

Cerebral salt wasting syndrome (CSWS) is a common cause of refractory hyponatremia. Early diagnosis and treatment of CSWS can greatly improve the patient′s condition and improve the prognosis. Clinically by supplying sodium hydrate, most patients can resume normal serum sodium level, and get remission. But a few patients with low serum sodium, high urine sodium and low blood volume can not improve and even worse. Fludrocortisone and adrenal cortical hormone can rapidly and effectively reduce the urinary sodium level and urine volume, correct hyponatremia and low blood volume and improve the prognosis in such patients.

Bladder cancer stem cells may give the cause of the recurrence and metastasis of bladder cancer patients,and CD47 mediated the suppress of cytophagy maybe the important mechanism of cancerous cells' immunologic escape.It is practical to isolate bladder cancer stem cells and cure the bladder cancer by targeting the CD47.In this paper,the authors will address the progress of bladder cancer stem cells and CD47 as therapeutic target.

Version 2015 of the National Comprehensive Cancer Network (NCCN) Guidelines for Uterine Neoplasms uses the 2009 International Federation of Gynecology and Obstetrics (FIGO) staging system.These NCCN guidelines divide pure endnmetrioid cancers into three categories to delineate treatment:(1) disease limited to the uterus;(2) cervical involvement;(3) extrauterinc disease.Surgery is recommended for medically operable patients.Continuous progestin-based therapy may be considered for highly selected patients with stage IA disease who wish to preserve their fertility.Preoperative chemotherapy can be considered if extrauterine disease is suspected.Factors that may influence the decision regarding adjuvant therapy include lymph vascular space invasion (LVSI),patient age,tumor volume,and lower uterine segment or surface cervical glandular involvement.When administering adjuvant radiotherapy,it should be initiated as soon as the vaginal cuff has healed,no later than 12 weeks after surgery.