Preliminary screening test and interfering test were used to study the bacterial endotoxin test (BET) for Shengmai Injection (SI). The results showed that there is no interference when the SI is diluted by 10 times and limulus reagent with a sensibility of 0.25EU/mL is used for BET.

Hepatocellular carcinoma (HCC) is a malignant tumor with high incidence and mortality rates around the world.Radiofrequency ablation (RFA) is an effective therapy for HCC,especially in patients who experience recurrence after surgery for liver cancer,who are complicated by liver dysfunction and unable to undergo surgical resection,and who have multiple liver tumors and undergo palliative treatment.RFA can significantly improve patients' survival time and quality of life.This article reviews the mechanism of RFA in the treatment of HCC from the aspects of direct and indirect injuries and helps to further understand the role of RFA in local and systemic treatment of HCC,which has a potential value for multidisciplinary treatment of HCC in future.

After a description of the concept of microvideo and studies on microvideo in domestic college and university libraries,microvideo in domestic college and university libraries was investigated and the production of microvideo was elaborated with Jining Medical College as an example.Strategies for the solution of problems in microvideo were put forward for its popularization.

Multiple risk factors lead to occurrence of cardiovascular diseases in common.Besides confirmed tradition-al risk factors,such as diabetes mellitus,dyslipidemia,smoking and hypertension etc.,some new risk factors have been found in recent years.The confirmed risk factors need further research,while the new risk factors need en-hanced study.

Heart failure is a serious and terminal stage of various cardiovascular diseases,is one of the most important heart diseases,and it also the most common reason for hospitalization.In recent years,studies on new markers in the diagnosis,treatment and prognosis of heart failure have made great progress,such as B-type brain natriuretic peptide can partially reflect hemodynamic imbalance,troponin can indicate cardiomyocyte injury,soluble ST2 and galectin-3 indicate myocardium remodeling and fibrosis,C-reactive protein,cystatin C and procalcitonin,and copeptin have significant prognostic value for outcomes,microRNA in the regulation of gene expression influence heart failure.

Equisetin (EQST) belongs to polyketide (PKS)-nonribosomal peptide synthetase (NRPS) type compound with an inhibitory effect of 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) enzyme activity. This study investigated anti-obesity effect and insulin resistance improvement effect of EQST on high-fat diet (HFD)-induced ob/ob mice model. EQST treatment effectively reduced the body weight gain, fat weight gain and blood lipid content of model mice. All animal experiments were approved by the Medical Ethics Committee of Capital Institute of Pediatrics. EQST alleviated adipose tissue expansion and hepatic ballooning degeneration of model mice, and also effectively controlled the blood glucose content after glucose load and insulin load, showed a significant improvement in obesity and insulin resistance. EQST inhibited adipogenic proteins fatty acid-binding protein 4 (FABP4) and peroxisome proliferators-activated receptor γ (PPARγ), and upregulated thermogenic protein (uncoupling protein 1, UCP1) through suppressing 11β-HSD1 protein expression. In addition, EQST widely upregulates mitochondrial respiratory metabolism related proteins in adipose tissue and may improve insulin resistance through phosphatidylinositol-3-kinase (PI3K) pathway. Therefore, EQST plays an anti-obesity role by promoting adipose tissue thermogenesis and improving insulin resistance, which may provide reliable clues for improving obesity and diabetes.

BACKGROUND: Pathological changes of form and structure are induced in some tissues during repairing albuginea penis trauma, while structure and component of collagen fiber will be correspondingly changed necessarily.OBJECTIVE: To find a convenient, shortcut, and sensitive method to observe the dynamic change of type and distribution of collagen fiber during albuginea penis healing. DESIGN, TIME AND SETTING: A contrast study was performed at the Laboratory of Tissue Engineering, Guiyang Medical College between June 2005 and October 2007. MATERIALS: Fifteen male New Zealand rabbits were used in this study. METHODS: All rabbits underwent an excision of albuginea sizing 5 mm×5 mm at dorsal penis to establish models of albuginea penis trauma, and then the excision was sutured in situ. Thereafter, albuginea penis was obtained from five rabbits once after 2, 6, and 12 weeks. The samples were treated with picric-sirius red staining and observed under polarizing microscope. MAIN OUTCOME MEASURES: Dynamic changes of area of collagen fiber during albuginea penis healing.RESULTS: There were significant differences in content of the same collagen fiber at different time points (P<0.05). At 2 weeks post-trauma, both type Ⅰ and type Ⅲ collagen fibers were observed, and the ratio was equal, but the arrangement was confused and disorder. At 6 weeks post-trauma, red and yellow type Ⅰ collagen fiber was apparently increased, but type Ⅲ collagen fiber was decreased, and the arrangement was still disorder. At 12 weeks post-trauma, thick, bright red and yellow type Ⅰ collagen fiber was rapidly increased, but thin and green type Ⅲ collagen fiber was rapidly decreased.CONCLUSION: Picric-sirius red polarized light method can not only differentiate types of collagen fiber, but also clearly display the distribution, form and proportion of type Ⅰ and type Ⅲ collagen fiber, characterizing by simple, convenient, specific, and highly sensitive.

The microRNA let-7 family,functioning as a tumor suppressor,has emerged as a regulator of stem cell differentiation and tumor repression.The RNA-binding protein lin28 is a stem cell pluripotency factor,which contributes to the maintenance of stem cell characteristics and promoting cell malignant transformation.lin28 can block the maturity of let-7 by combining with the precursors of let-7 family miRNAs,which is itself able to repress lin28 by binding to the 3'-UTR of lin28 transcripts,thus forming a feedback loop.The double-negative feedback loop comprising lin28 and let-7 is involved in several biological processes such as differentiation of stem cell,tumorigenesis,invasion and metastasis,drug resistance and relapse,which couldbe a potential target for cancer therapy.

Objective To study the negative emotion regulation of sea-going seafarers,establish a regulation strategy questionnaire of sea-going seafarers' negative emotion and examine its reliability and validity.Methods Based on open-ended questionnaires and previous researches,the initial questionnaire was obtained.Randomly selected 188 sea-going seafarers,by adopting program analysis and factor analysis,the final questionnaire was determined.Tested 121 sea-going seafarers by using the final questionnaire,while 55 of them by using coping style questionnaire at the same time.After two weeks,47 of them were tested by final questionnaire again.The reliability and validity of the questionnaire were investigated during the research,and the criterion validity between the coping style questionnaire and regulation strategy questionnaire of sea-going seafarers' negative emotion was also studied.Results The factor analysis results showed that the questionnaire was composed of seeking for support,abreaction,inobservance,transfer and oppression.Cronbach α of each factors was 0.857,0.841,0.825,0.806,0.754 respectively;and Cronbach o of the entire questionnaire was 0.873.The split-half correlation coefficients were 0.697,-0.839 (P ＜ 0.01) ; test-retest reliability coefficients were 0.706,-0.822 (P ＜ 0.01).The correlation between subscales were-0.031,-0.459 ; and the correlation between subscales and total scale were 0.590,-0.806 (P ＜ 0.01).Criterion validity showed that despite for the abreaction,each factor was significantly correlated with the factors of coping style questionnaire.Conclusion The sea-going seafarers' negative emotion regulation strategy questionnaire has acceptable psychometrics properties on reliability and validity.

OBJECTIVE:To study the bioequivalence of Alendronate Sodium Tablets in healthy volunteers. METHODS:According to a paired and cross-over design,a bioequivalence test was performed on two different alendronate sodium tablets,10 mg (Group 1) and 70 mg (Group 2). Then each group was subdivided into two groups,i.e. test preparation and reference preparation. The urine concentrations of alendronate at different time point within 48 h were determined by HPLC and the urine excretion rate -time curve was drawn,and the DAS software was used to compute the pharmacokinetic parameters and the relative bioavailability of the tested formulation. RESULTS:The 90% confidence intervals of urine excretion rate of the tested formulations were 82.4% ～122.6% (Group 1) and 85.4% ～124.6% (Group 2),respectively and the maximum excretory rates were 80.3% ～131.5% (Group 1) and 82.3～136.5% (Group 2),respectively,compared with the reference formulations. CONCLUSION:The two different Alendronate Sodium Tablets and their respective reference formulations were proved to be bioequivalent in healthy volunteers.