Objective:To investigate the cardiac magnetic resonance technique in the diagnosis and treatment of unstable angina application value. Methods:The hospital diagnosed patients with unstable angina as research subjects, were given coronary angiography in patients (CAG) examination, ECG, echocardiography and cardiac MRI examination, recording the results and statistical analysis. Results:Cardiac MRI for>90%stenos is detected heart rate, and coronary angiography (CAG) showed no significant difference (x2=3.257, P>0.05), for<90%stenos is detection rate, and coronary angiography (CAG) was statistically significant between test results (x2=17.267, x2=25.714;P<0.05);cardiac magnetic resonance for the degree of stenos is of myocardial ischemia in patients with high detection rate and electrocardiogram detection rates were significantly different(x2=4.65, P<0.05), but the total no significant difference between the detection rate(x2=0.251, P>0.05);cardiac MRI for cardiac function and cardiac structure determination is better than conventional echocardiography. Conclusion: Cardiac MRI technology to more accurately reflect changes in cardiac structure and function of the stenos is and effort ischemia detection rate with conventional inspection methods have better consistency.

Objective To investigate the effect of Urinarykallid for different ages patients with acute cerebral infarction cases.Methods Ninety-five cases with acute cerebral infarction were divided into high age group(n=48) and middle age group(n=47).Patients in the two groups were both given the Urinarykallid therapy combined with routine treatment.After 1 courses of treatment(3 weeks),American National Institutes of Health Stroke Scale(NIHSS) were performed before and after the treatment.The adverse reactions occurred in the process of scoring were compared.Results The total effective rate in the high age group was 83.33％,lower than the middle age group(89.36％),but the difference was not statistically significant(P=0.391).The NIHSS scores of patients in the two group after treatment were both significantly reduced compared with that in before treatment (high age grou:(9.81±2.37) vs.(6.79± 0.82),middle age group:(9.75 ± 2.46) vs.(3.04±0.58;P=0.004,P=0.001).Meanwhile,the NIHSS in the middle age group decrease significantly than the high age group(P=0.000).No obvious adverse reactions occurred in both two groups,the treatment was proved with safe and reliable.Conclusion Urinarykallid treatment is showed with better therapy effect on acute cerebral infarction patients with different age,and it is safety with improving patients NIHSS score level.Meanwhile,the better efficacy is showed in patients with lower age.

MicroRNAs(miRNAs) are endogenous non-coding RNAs,about 20 nucleotides in length.They play a pivotal role in the regulation of genes involved in diverse biology processes such as cell development,proliferation,differentiation and apoptosis by the translation repression or mRNA degradation.Recent evidence has suggested that miRNA alterations are involved in the initiation and progression of various human cancer including hepatocellular carcinoma(HCC),and miRNA-expression profiling of HCC has identified signatures associated with diagnosis,staging,progression and prognosis.As a novel molecular target,miRNAs holds great promise in diagnosis and biotherapy of HCC.

Circulating nucleic acids (CNAs) are extracellular nucleic acids found in cell-free serum,plasma and other body fluids from healthy subjects as well as in patients. The ability to detect and quantitate specific DNA and RNA sequences has opened up the possibility of diagnosis and monitoring of diseases,especially in the field of cancer. Furthermore,in some recent studies it has been suggested a kind of non-coding RNA-microRNA (miRNA),also exist in cell-free serum and plasma,highlighting the field of using CNAs to diagnose cancer. As a novel tumor marker,tumor-specific circulating miRNAs holds great promise in early diagnosis of cancer.

AlM:To compare the efficacy and complication of extra-trabeculotomy in combination with trabeculectomy and trabeculectomy and compound trabeculectomy in the treatment of primary infantile glaucoma.
METHODS: Patients with primary infantile glaucoma undergone one of the three procedures from Jan 2006 to Jan 2014 were selected. Among them, group A ( 20 patients, 31 eyes ) underwent extra- trabeculotomy in combination with trabeculectomy, group B ( 20 patients, 32 eyes ) underwent trabeculectomy, while group C ( 20 patients, 30 eyes) underwent compound trabeculectomyRESULTS:The success rates in group A were 96. 8% at the time of discharge and 90. 3% during follow-up (mean 24mo) ; the rates in group B were 96. 9% and 81. 2%( mean 24mo) the rates in group C were 83. 3% and 76. 7%( mean 24mo ) respectively, the differences being significant (P <0. 05). ln group A and group B, there was no severe complications, while in group C, one case had vitreous prolapse.
CONCLUSlON: Extra - trabeculotomy in combination with trabeculectomy is more efficacious and safer than trabeculectomy and compound trabeculectomy in the treatment of primary infantile glaucoma. lt should be the first choice for primary infantile glaucoma.

Addition of oxygen-vectors(n-dodecane,n-hexane,hydrogen peroxide) to fermentation medium was recognized as a method of enhancing oxygen transfer and promoting lycopene yield by Blakeslea trispora fermentation.Higher lycopene production was observed in shake flask containing 1%(v/v) n-hexane,n-dodecane and 150 L/100mL hydrogen peroxide as compared to shake flasks without oxygen-vectors.The result of assays indicated that when oxygen-vectors (n-dodecane,n-hexane,1%,v/v) and hydrogen peroxide(50 L/100mL) were added to the 0-day and 1-day old culture of Blakeslea trispora the production of lycopene were 25.32%,72.84% and 40.35% higher than those without oxygen-vectors addition respectively.The production of lycopene increased 114.83% when n-dodecane and surface active agents were used at the same time.

Lycopene—a kind of important active compound of caroteinoids, is greatly beneficial to human health with its diverse biological functions. With the elucidation of lycopene biosynthetic pathway and cloning genes of relative enzymes from microorganisms, it is possible to regulate lycopene biosynthesis via genetic engineering. The biosynthesis pathways of lycopene and gene cloning of lycopene biosynthetic enzymes in microorganisms were reviewed, and gene engineering strains documented in previous works including: E.coli and yeast constructed by genetic recombination, mold strains enhanced the ability of producing lycopene by gene manipulation were summarized. At last, compared with the present methods, the problems existed in the process of construction were pointed out.

To establish a spectrophotometric method for detecting selenium in drinking water,some subjects,such as the features of absorption spectrum of the reacting product of selenium and diaminonaphthalene,disturbance of other coexisting ions,detection range,detection limit,precision and accuracy were studied.The results showed that:the maximum absorption wavelength of the reacting product of Se4+and 2,3-diaminonaphthalene was 377 nm,the detection limit,molar absorptivity (ε377) and linear range of this method was 0.018 μg/50 ml,2.7×104 L/(mol·cm)and 0.2～10 μg/50ml respectively.The regressive equation was A＝0.077C＋0.014 with a correlation coefficient r value of 0.9999.The relative standard deviation (RSD) and recovery rate were ＜54.7% and 96.5%～106% respectively.This method was simple,sensitive and accurate,could meet the requirement of the routine monitoring of selenium in drinking water.

Objective To investigate calcification of cultured aortic medial cells in vitro and acceleration by 25 hydroxycholesterol or ? glycerophosphate. Methods Aortic medial cells were obtained by explantation, and the calcification was observed by von Kossa staining. Insoluble calcium precipitation in cellular layer was determined by biochemical method , and osteocalcin in the media was analyzed with radioimmunoassay. Results Two different types of primary cells were shown from culture: one with parallel cellular growth and being negative by von Kossa staining, the other cell type formed cellular nodules with positive von Kossa staining. After 28 days of cell passages, cell growth appeared no nodule formation. However, many cellular nodules and positive von Kossa staining were observed in the passaged cells treated with 25 hydroxycholesterol or ? glycerophosphate, and both insoluble calcium 〔(57 80?18 50)?g/pool, (67 50?15 30)?g/pool〕and osteocalcin 〔(0 886?0 063)?g/L, (0 895?0 061)?g/L〕in the medium were significantly increased than that of the untreated cells. Conclusions Cultured aortic medial cells could be divided into two subtypes, one with the characters of smooth muscle cells, the other with the micro vascular pericytes which could calcify the extracellular matrix. 25 hydroxycholesterol and ? glycerophosphate promoted the in vitro calcification, and osteocalcin secretion was increased in the process of calcification of aortic medial, suggesting that osteocalcin might participate in the aortic calcification.

Objective To discuss the application of surface anatomy scanning in the department of cerebral surgery. Methods 24 cases were examined by surface anatomy scanning.These included 5 normal cases,10 gliomas,4 metastasis tumors,3 meningiomas,1 cavernous hemangioma and 1 cerebral infarction.Results 5 normal cases with surface anatomy scanning showed excellent visualization of the sulci and gyri on the surface of the brain.In 19 cases with cortical or subcortical lesions,14 cases showed the relationship between the lesion and the brain surface structures well.5 cases could not displayed because of server edema around the lesion.Conclusion The brain surface anatomy scanning is useful for the localization of cortical and subcortical pathology,for the diagnosis of the disease of sulci and gyri,can partly replace the navigation.