2.A controlled study of hemodialysis combined with hemoperfusion for the treatment of microinflammation in diabetic nephropathy
Chongqing Medicine 2017;46(6):767-769
Objective The aim of this study was to assess the effect of hemodialysis combined with hemoperfusion for the treatment of microinflammation in diabetic nephropathy patients.Methods One hundred and twenty-five cases of diabetic nephropathy experienced treatment from July 2014 to December 2015 in author's hospital were analyzed retrospectively.They were divided into two groups randomly.The observation group received hemodialysis combined with hemoperfusion,and the control group received just hemodialysis therapy.The status of microinflammation and curative effect were compared.Results The effective rate of observation group was 60.6%,while was 45.8% in control group (P<0.05).Microinflammation level:compared with control group,SAA,IL-6,IL-17,IL-22,IL-1β,TNF-αand TGF-β1 status of observation group showed decreased trend (P<0.01).Conclusion Hemodialysis combined with hemoperfusion can effectively decreased microinflammation status in diabetic nephropathy.
3.Meta-analysis of the effect of intravitreal anti-VEGF drugs versus intravitreal triamcinolone in macular edema caused by central retinal vein occlusion
Chinese Journal of Experimental Ophthalmology 2016;34(12):1097-1101
Background Central retinal vein occlusion (CRVO) is a common retinal vascular disease.Macular edema is a common complication and can lead to the decrease of visual acuity.Intravitreal injection of antivascular endothelial growth factor (VEGF) drugs and triamcinolone acetonide has become the important treatment on macular edema.Objective This study was to systematically evaluate the clinical effects of anti-VEGF drugs and triamcinolone in patients with macular edema caused by CRVO.Methods The Databases including PubMed,Cochrane Library (Issue 11,2012),EMbase,CBM,CNKI,VIP and WanFang Database were electronically searched for the trials about the effects of anti-VEGF drugs and triamcinolone in patients with macular edema caused by CRVO from the date of establishment of the databases to September 2015.The combined effect was analyzed by using Review Manager 5.3 software.Results A total 7 trials involving 345 patients and 348 eyes were included.Meta-analysis showed that there was no statistical difference in best corrected visual acuity (BCVA) and macular central thickness between anti-VEGF drugs and triamcinolone in the 6-month follow-up (mean difference [MD] =-0.03,95% confidence interval[CI]:-0.11-0.05,P =0.52;MD =-15.37,95% CI:-36.29-5.55,P =0.15),but there was statistical difference in intraocular pressure (MD =-2.73,95% CI:-3.59--1.86,P<0.000 01).Twenty-two cases of lens opacity and 8 cases elevated intraocular pressure were observed in the triamcinolone group.Only 2 cases of lens opacity were observed in the anti-VEGF drugs group.Conclusions Anti-VEGF drugs and triamcinolone have similar improvement of BCVA and decrease of macular central thickness in CRVO patients,while the triamcinolone is accompanied with more side effects such as high intraocular pressure and progressing cataract.
5.Effect of intrathecal gastrodin on skin cancer pain in mice
Jia HUA ; Hua WANG ; Peng MA ; Aihua GONG ; Peng JIANG
Chinese Journal of Anesthesiology 2016;36(5):588-590
Objective To investigate the effect of intrathecal gastrodin on skin cancer pain in mice.Methods Thirty-two female Balb/c mice,aged 8 weeks,weighing 20-25 g,were randomly divided into 4 groups (n=8 each) using a random number table:sham operation group (group S),skin cancer pain group (group SCP),gastrodin group (group G),and artificial cerebrospinal fluid (ACSF) control group (group ASCF).Skin cancer pain was produced by injecting phosphate buffer solution 20 μl containing about 2 ×105 4T1 breast cancer cells into the plantar surface of the left hindpaw.At 14th day after inoculation of cancer cells,ASCF 5 μl was injected intrathecally in S and ACSF groups,and gastrodin 150 μg/kg (5 μl) was injected intrathecally in group G.Before inoculation,at 30 min before intrathecal injection,and at 15,30,60,90,120 and 150 min after intrathecal injection,the thermal paw withdrawal latency (TWL) was measured.The expression of acid-sensing ion channel (ASIC)-3 mRNA in the spinal dorsal horn was detected using the real-time reverse transcriptase polymerase chain reaction after the last measurement of the pain threshold.Results Compared with group S,the TWL was significantly decreased at each time point before and after intrathecal injection in SCP,ACSF and G groups,and the expression of ASIC-3 mRNA in the spinal dorsal horn was significantly down-regulated in group G (P<0.05).Compared with group SCP,the TWL was significantly increased at each time point after intrathecal injection,and the expression of ASIC-3 mRNA in the spinal dorsal horn was significantly down-regulated in group G (P<0.05),and no significant change was found in the parameters mentioned above in group ACSF (P>0.05).Conclusion Intrathecal gastrodin can reduce skin cancer pain and down-regulate ASIC-3 expression in the spinal dorsal horn which is helpful in maintaining the analgesic effect in mice.
6.The influence of bFGF gene transfected BMSCs on inflammatory cytokines expression of COPD rat
Peng WANG ; Qiang NIE ; Lin MA ; Shanshan PENG ; Xin GAN
The Journal of Practical Medicine 2016;32(3):355-358
Objective To study the influence of bFGF gene transfected bone marrow-derived mesenchymal stem cells (BMSCs) on the inflammatory cytokines of COPD rat. Methods The BMSCs were separated from SD rat and cultured and then bFGF gene was imported to BMSCs by liposome transfection method. The samples were prepared into six groups: normal control group, COPD group (A), BMSCs group (B), pcDNA3.1-BMSCs group (C), bFGF-pcDNA3.1-BMSCs group (D), and bFGF group (E). The expressions of TNF-α and IL-1β by QRT-PCR were detected. Results Compared with COPD group, TNF-α and IL-1β genes from groups B to D dropped significantly (P < 0.05). The changes of TNF-α and IL-1β genes among groups B to D showed no significant difference (P > 0.05). Conclusion BFGF transfected BMSCs, sample BMSCs and pcDNA3.1 transfected BMSCs can inhibit the expression of inflammatory cytokines of TNF-α and IL-1β, but there is no obvious advantage in comparison to bFGF transfected BMSCs and sample BMSCs in respect of inhibiting the expression of inflammatory cytokines of TNF-α and IL-1β.
7.Clinical outcomes comparison of unipedicular kyphoplasty versus bipedicular vertebroplasty treating for osteoporotic vertebral compression fracture
Xinfeng CAO ; Guodong PENG ; Ming PENG ; Xiaocheng MA
Chinese Journal of Postgraduates of Medicine 2011;34(32):17-19
Objective To compare the clinical outcome of unipedicular versus bipedicular vertebroplasty treating for osteoporotic vertebral compression fracture.Methods Sixty-four patients(68 vertebra)were divided into two groups by treated methods:unipedicular kyphoplasty group(33 cases)and bipedicular vertebroplasty group(31 cases).The Cobb angle and vasual analogue pain scale(VAS)were measured preoperatively and postoperatively.The operation time was recorded.Compared the Cobb angle,VAS and the operation time between two groups.Results Of unipedicular kyphoplasty group preoperative,24 hours and 3 months after operation,VAS were(8.42 ± 1.33),(2.21 ± 1.67),(2.09 ± 1.58)scores,the Cobb angle were(31.24 ±9.12)°,(14.21 ±9.21)°,(14.43 ±9.36)° ;while those of bipedicular vertebroplasty group were(8.36 ± 1.52),(2.13 ± 1.80),(2.00 ± 1.71)scores and(30.84 ±8.77)°,(13.94 ± 8.87)°,(14.07 ± 9.87)°.VAS and the Cobb angle of both groups at 24 hours and 3 months after operation were lower than those preoperative(P< 0.01).VAS and the Cobb angle of both groups were similar at the same time preoperatively and postoperatively(P > 0.05).The operation time of unipedicular kyphoplasty group and bipedicular vertebroplasty was(45.00 ± 8.76),(72.00 ± 9.32)min,respectively,there was statistically significant difference between two groups(P < 0.01).Conclusions Compared with the bipedicular vertebroplasty,the advantages of unipedicular kyphoplasty are as follows:less trauma,less operation time and less X-rays rediation accepted of the patient and the operator.And it has the similar clinical outcome with the bipedicular vertebroplasty.
8.Impact of storage conditions and time on herb of Lonicera macranthoides.
Peng MA ; Long-Yun LI ; Ying ZHANG
China Journal of Chinese Materia Medica 2014;39(6):981-991
To study the effect of different storage conditions and storage time on herb quality of Lonicera macranthoides, different packaging materials including vacuum plastic bags, plastic bags, woven bags, sealed with endometrial bags, paper bags, sack bags were selected for the study under different storage conditions including room temperature, 5 degrees C refrigerator, low temperature of - 20 degrees C refrigerator and desiccator. Twenty-four batches of samples were used for the study, and active ingredients were determined. The experimental results showed that the ingredients in each storage group changed with the storage time, storage conditions (storage environment, packaging). Under the same storage time, the storage environment (temperature, humidity) had effect on the stability of herb quality. Low temperature had less effect on herb quality. The effect of packaging on herb quality was as following: plastic vacuum packaging > woven with endometrial sealed packaging > plastic bag > woven bag > sack bags > paper bags. Under the same storage conditions, with the increase of storage time, caffeic acid content increased slowly, and other five ingredients content decreased gradually. Storage time affected significantly on the intrinsic quality (chemical composition) and appearance of herb. It is suggested that low temperature (5 degrees C), dark and sealed storage are suitable for storage of L. macranthoides herb, the storage time should be not more than 24 months.
Desiccation
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Drug Packaging
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Drug Storage
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methods
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Drugs, Chinese Herbal
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chemistry
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Lonicera
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chemistry
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Organic Chemicals
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analysis
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Quality Control
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Time Factors
9.Single nucleotide polymorphisms and prostate cancer.
Chao MA ; Chun-Xiao LIU ; Peng HUANG
National Journal of Andrology 2014;20(8):738-742
Prostate cancer is a common malignancy that affects men's health in the Western countries. Single nucleotide polymorphisms (SNPs), as the third generation of genetic markers, can influence the development, progression, and prognosis of prostate cancer. The same SNP may be related differently with prostate cancer among different races. This paper describes the relationship between SNPs and prostate cancer according to their related genes. SNPs can predict the risk of prostate cancer as well as the possible adverse reactions in its treatment, but at present they do have some limitations.
Humans
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Male
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Polymorphism, Single Nucleotide
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Prostatic Neoplasms
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genetics
10.Application and preventive effect of hammock firmness sheets for pressure ulcer
Mengling MA ; Cuiping DAI ; Yanmei PENG
Chinese Journal of Practical Nursing 2010;26(4):34-35
Objective To know the preventive effects of using hammock firmness sheets for pa-tients with pressure ulcer.Methods Divided 98 patients into the application group (31 cases), the treatment group(30 cases) and the control group(37 cases) according to themselves condition.Hammock firmness sheets was used in the application group, while the routine preventive method of pressure ulcer was used in the control group.To know the preventive effects of pressure ulcer betweent the two groups.The patients in the treatment group was patients had pressure ulcer, hammock firmness sheets combined with local massage were used for them, observed the treatment effects for them.Results The condi-tion of preventive effect of pressure ulcer in the application group was better than those of in the control group.The cure rate of pres sure ulcer in the treatment group was 90%.Conclusions The hammock firmness sheets can prevent pressure ulcer effectively.