Objective: To analyze the imaging findings, diagnosis and treatment of central neurocytoma. Methods: The clinical information of 7 cases of central neurocytoma in Second Hospital of Hebei Medical University from January 2005 to March 2013 was retrospectively analyzed. The surgical experience was summarized, and the related literatures were reviewed. Results: Of 7 cases, 3 were females and 4 were males; 6 had tumor in anterior 2/3 of the ventricle, 5 originated from septum pellucidum around monro foramen, 1 originated from lateral ventricular wall. The MRI showed that the lesions were mostly hypointense and displayed mixed signals on T1WI images, mainly hypointense and slightly hyperintense signals on T2WI images; most of neurocytomas showed heterogeneous enhancement on dynamic contrast-enhanced MRI images. Six cases underwent gross total resection, 1 case underwent subtotal resection. Two cases received postoperative radiotherapy, 1 case received adjuvant chemotherapy. One case died postoperatively in hospital. Five cases were followed-up, and 1 case was lost to follow-up. The follow-up time was from 15 to 25 months. Four cases had satisfying prognosis, 1 case had hemiplegic paralysis, and no cases had recurrent tumor. Conclusion: Microneurosurgery is the best treatment for central neurocytoma, mastering the technique and key points of operation can achieve significant clinical effectiveness and improve the prognosis. Copyright © 2013 by TUMOR.

Objective To investigate the effect of dynamic regulation of negative pressure values in vacuum sealing drainage (VSD) on healing of soft tissue wounds.Methods From January 2013 to December 2015,115 patients were treated at our institute for open soft tissue defects of Gustilo-Anderson type ⅢaⅢc.They were 69 males and 46 females,aged from 17 to 76 years (average,44.7 years).They were divided into 4 groups to be subjected to different negative pressure values.Group A (n =29) was subjected to negative pressure values from-40 to-20 kPa,group B (n =29) to values from-80 to-60 kPa,group C (n =29) to values from-40 to-20 kPa for the first 72 hours followed by values from-80 to-60 kPa,and group D (n =28) to values from-60 to-80 kPa for the first 72 hours followed by values from-40 to -20 kPa.The 4 groups were compared in terms of drainage volume,wound shrinking,cleaning time,healing time and complications.Results The drainage volumes on the 7th day in groups B and D (4.89 ± 0.66 mL/cm2 and 4.33 ± 0.96 mL/cm2) were significantly higher than those in groups A and C (2.90 ± 0.67 mL/cm2 and 2.99 ±0.56 mL/cm2);the wound shrinking areas on the 14th day in groups B and D (14.16 ± 1.77 cm2 and 13.84 ± 1.65 cm2) were significantly larger than in groups A and C (12.57 ± 1.92 cm2 and 10.95 ± 1.37 cm2) (P ＜ 0.05).The cleaning time in group A was significantly longer than in the other 3 groups (P ＜ 0.05).The healing time in groups A and C was longer than in groups B and D (P ＜ 0.05).The incidences of pain in groups B and C were significantly higher than in groups A and D (P ＜ 0.05).The incidences of infection and blocking in group A were higher than in the other 3 groups (P ＜ 0.05).Conclusion Dynamic regulation of negative pressure values from-80 to-60 kPa for the first 72 hours followed by values from-40 to-20 kPa is an optimal choice for VSD management of soft tissue defects due to adequate drainage,obvious wound shrinking,quick cleaning and healing,and limited complications.

ObjectiveTo investigate the efficacy and safety of the self-expandable metal stent for treatment of gastric outlet and duodenal obstruction.MethodsData of 24 patients with symptomatic gastric outlet and duodenal obstruction diagnosed from January 2005 to February 2011 were retrospectively studied.All patients were measured by gastric outlet obstruction scoring system ( GOOSS ) before and one week after the placement of self-expandable metal stent.ResultsStent placement was succeeded in all patients with a mean operation time of 25.90 ± 11.03 min.Twenty patients (83.3％) were able to intake liquid food after the procedure.The GOOSS (P50 =0) before and after stent placement were 0.14 (P50 =0) and 1.42( P50 =2),respectively,with significant difference (P ＜0.01 ).Minor digestive bleeding occurred in 3 patients,but no severe complications including perforation or aspiration were observed.ConclusionPlacement of a self-expandable metal stent in patients with gastric outlet or duodenal obstruction is safe and effective for resolving obstructive symptoms.

BACKGROUND: Cryotherapy of acute soft tissue injury has been widely used in clinical practice.OBJECTIVE: To observe the histological changes and treatment effect of different cryotherapies on the rats' acute damage of soft tissue. METHODS: Neonatal Wistar rats were randomized to normal, model, intermittent cryotherapy and continuous cryotherapy groups. Models of acute damage of soft tissue were established in model, intermittent cryotherapy and continuous cryotherapy groups. In intermittent cryotherapy group, the injury was treated by intermittent cryotherapy with ice bag at 4 °C; in the continuous cryotherapy group, the injury was treated by continuous cryotherapy with ice bag at 4 °C; the model group was not treated. Histological changes were observed at 48 hours. Injury degree was evaluated using injury symptom index.RESULTS AND CONCLUSION: Compared with model group, the scores of injury symptom index and histology were lower, interleukin-1β expression was reduced, and transforming growth factor-β1 (TGF-β1) expression was increased in intermittent cryotherapy and continuous cryotherapy groups (P < 0.05). Compared with intermittent cryotherapy group, the scores of injury symptom index and histology were reduced (P < 0.05), interleukin-1β expression was reduced (P < 0.05), and TGF-β1 expression was increased in continuous cryotherapy group (P < 0.05). Results demonstrated that cryotherapy can cure the acute damage of soft tissue by reducing interleukin-1β expression and raising TGF-β1 expression. Continuous cryotherapy is superior over intermittent cryotherapy.

The study is aimed to confirm the silencing efficiency of the vector in human hepatocellular liver carcinoma cell line (HepG2), and observe effects of AMPKgamma silencing on the AMPK stimulating activity and lipid synthesis of cordycepin (CCS), a natural product with known AMPK activating function. The downregulating efficacy of siRNAs on AMPKgamma expression was confirmed in our previous study. The double stranded shRNA Oligo was ligated to lentivirus vector and verified by sequencing. The lentiviral which can effectively inhibited protein expression levels of AMPKgamma was selected by Western blotting, and the regulation of CCS on protein expression of AMPKgamma and p-AMPK in AMPKgamma silence cells were detected by Western blotting analysis. The lipid accumulation in cells was observed by Oil-Red O stain and cells were collected for the estimation of cholesterol (TC), triglyceride (TG). The results showed that the lentiviral vector carrying a shRNA targeting the AMPKgamma gene was successfully constructed. Western blotting analysis confirmed that GR085 had the highest interfering efficiency. Treatment with CCS can significantly increase the levels of phospho-AMPK in normal cells, and the level of TC, TG was reduced, but in AMPKgamma silence cells the effects of CCS on AMPK activation and lipid synthesis were almost completely abolished without changing the expression levels of total AMPK or AMPKgamma protein. In conclusion, the AMPKgamma gene may be related to AMPK activation and intracellular lipids regulation by CCS.

The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells.Recently,recombinant baculoviruses containing mammalian cell-active promoter element have been used to transduce a broad spectrum of primary and established mammalian cells,including non-hepatic cells.Recombinant baculoviruses have been used successfully for transient or stable gene delivery in mammalian cells in vitro,while the efficiency of delivering gene in vivo is inhibited obviously by complements,but efforts have been made to overcome the problems,for instance,VSV-G-pseudotyped baculoviruses display complement resistance.The mechanism of the transduction is still not clearly understood,though many researches have been done.The baculovirus is able to replicate only in insect cells,but it is incapable of initiating replication cycle in mammalian cells,which guarantees high biosafety of this gene delivery system.In addition,this system is easily manipulated and able to carry large inserts.These attributes will undoubtedly lead to the increased application and continued development of this system for highly effective gene delivery into mammalian cells.

Objective To analyze the utilization of community health services(CHS) and influencing factors in Shenzhen. Methods Surveys were conducted by means of cluster random sampling and utilization of CHS for health promotion, utilization of single services and integrated utilization were analyzed using descriptive methods. Results The rate of integrated utilization was 35.90% and the most utilized single services were patient care and drug purchase, with general physical checkup, health guidance and disease prevention coming next. Conclusion Factors such as the layout, convenience and knowledge of CHS are conducive to enhancing the rate of CHS utilization whereas factors such as residence in a special economic zone, student status, and higher educational backgrounds have a negative influence on CHS utilization.

Bone growth factors play an important role in the process of bone metabolism and perform a variety of physio -logical functions such as regulating bone growth , differentiation and remodeling .Meanwhile , these cytokines are associated with orthopedic disorder and therapy .The curative effect of the overturned sartorius iliac flap in repair of acetabular defect in developmental dysplasia of the hip proves to be good , but mechanism between the overturned flap and the surrounding bone tissue is unclear .This review briefly summarizes the functions and applications of several major cytokines in bone for -mation and remodeling , which may provide new ideas and methods for illuminating the mechanism of these cytokines during the repair of the acetabular defect .

ObjectiveTo evaluate the effect of radical proctectomy with TME for the treatment of rectal carcinoma in the prevention of postoperative local recurrence. Method78 patients undergoing this procedure were followed up for 5 years.ResultLocal recurrence developed in 10 cases during 5 year′s follow up, with recurrence related mortality of 7/78, local recurrence rate of 10/78 (13%). Tumor malignancy and Dukes′ grade were in direct proportion with the recurrence. The 5 year′s survival rate was 88%(69/78). The postoperative 5 year′s survival rate in Dukes′ A, B and C patients was respectively 100%(9/9), 92%(51/54), and 64%(9/14). ConclusionRadical proctectomy with TME is superior to that without TME in terms of low postoperative local recurrence rate for the treatment of rectal carcinoma.

Objective To investigate the analysis of low calcium intravenous calcium supplementation agent and active vitamin D3 on total thyroidectomy on quality of life in patients with nodular goiter. Methods 40 cases of nodular goiter with total thyroidectomy were performed in our hospital from March 2014 to September 2016. It was randomly divided into control group and experimental group, 20 cases in each group. The patients in the control group were nodular thyroidectomy and the experimental group underwent total thyroidectomy. The quality of life of two groups of patients was evaluated by using the European five-dimensional health-related living quality scale (eq-5d). Results Two groups of patients, relevant questionnaire survey of the patient's ability to act, self-care ability, daily activity ability, pain or discomfort, anxiety, or depression, and the comparison of VAS score, according to the results of difference was not statistically significant. In comparison with ordinary people, the questionnaire survey results showed that the proportion of patients with moderate or above differences was higher and the difference was significant (P< 0.01). There was no statistical significance compared to the average person. Conclusion All patients with nodular goiter are treated with total thyroidism, and the effect is good, and the quality of life of patients is relatively small.