Endometrial Neoplasms ; classification ; epidemiology ; mortality ; pathology ; Female ; Humans ; Uterine Cervical Neoplasms ; classification ; epidemiology ; mortality ; pathology

Objective To investigate the effects of Rehmannia glutinosa polysaccha-rides (RPS) on myocardial apoptosis of rats after myocardial ischemia-reperfusion injury. Methods The experimental rats were randomly devided into sham operation group, model group, RPS low, medium and high-dose group, Danshen tablets group, 20 rats in each group. All the rats were performed the coronary occlusion operation except the sham operation group. And then, the rats in RPS low, medium and high-dose groups were given RPS 10, 20, 40 mg/kg by intragastric administration;the rats in Danshen tablets group were given Danshen tablets suspension 260 mg/kg;the rats in sham operation group and model group were given the equal volume of saline;once a day for 4 weeks. The areas of myocardial infarction were analysised by TTC staining;the apoptosis of cardiomyocytes was observed by TUNEL and the apoptosis index(AI) were calculated;the expression of bcl-2, Bax, caspase-3 were detected by IHC and were semi-quantitative analysised. Results Compared with the model group, the areas of myocardial infarction (31.7% ± 6.1%, 22.8% ± 5.5% vs. 43.9% ± 6.7%) and the apoptosis index (44.6%± 6.7%, 32.8%± 5.4%vs. 59.4%± 9.0%) in RPS medium and high-dose group (RPS 20, 40 mg/kg) significantly decreased (P<0.05 or P<0.01). The expression of bcl-2 (0.40 ± 0.09, 0.45 ± 0.08 vs. 0.26 ± 0.05) significantly increased (P<0.05 or P<0.01), the expression of Bax (0.55 ± 0.11, 0.28 ± 0.08 vs. 0.85 ± 0.13) and caspase-3 (0.11 ± 0.03, 0.08 ± 0.02 vs. 0.16 ± 0.03) significantly decreased (P<0.05 or P<0.01), and the ratio of bcl-2/Bax (0.73 ± 0.05, 1.61 ± 0.20 vs. 0.31 ± 0.08) significantly increased (P<0.05 or P<0.01). Conclusions RPS had inhibition effects on myocardial apoptosis after myocardial ischemia-reperfusion injury in rats, which may be related to its effects of enhancing the ratio of bcl-2/Bax and down regulating the expression of caspase-3.

Objective:To study the fluorescent manifestations of fundus oculi lesion in several types of retinal degeneration.Methods:Fundus fluorescein angiography(FFA) were performed in 8 patients(16eyes)of retinal degeneration.Results:Lesion of eyes was actinomorphic,and was relatively quiet,grade was low,mainly presented as retinal pigment epithelium(RPE) and choroidal capillary atrophy.Background image choroidal at early stage showed hypofluorescence,and big choroid blood vessel low fluorescence were seen.This differed from secondary retinitis pigmentosa.Conclusion:Results of FFA with retinal degeneration show barrier of choroidal capillary atrophy may be one factor for RPE and neurosensory retina dystrophy.

Objective:To examine the effects of glucose on the expression of matrix metalloproteinases-2(MMP-2)and the changes of ultramicrostructure,cell cycle in cultured human umbilical vein endothelial cells.Methods:Human umbilical vein endothelial cells(hUVEC)were incubated in a culture medium with 11.2mmol/L,16.8mmol/L and 33.6mmol/L glucose concentrations for 24h,48h,72h respectively.The expression of MMP-2 was measured by RT-PCR and Western-blot.Results:When D-glucose concentrations rose,hUVEC exposed to high glucose concentration(11.2mmol/L,16.8mmol/L and 33.6mmol/L)increased in cell expression of MMP-2 after 48 h and 72 h of exposure,as compared with those cultured in medium with a low glucose concentration(5.6mmol/L).And cell cycle changed too.Conclusion:High concentration of glucose can arrest the proliferative response and show that even a short-term exposure of endothelial cells(ECs)to high glucose concentration leads to their activation associated with increased expression of MMP-2 and that this effect may appear more significantly along with time of exposure to high concentration of glucose.

Objective:To assess the clinical setting of a heavy silicone oil tamponade(Densiron-68) for rhegmatogenous retinal detachment(RRD).Methods:Twenty-nine eyes of 29 consecutive patients were recruited between December 2007 and February 2009.Primary vitrectomy with Densiron-68 was used in all cases.Patients to be selected had two groups:one group had 18 cases,mean age was 72.5 years.These patients were impossible prone because of ages and body factors.Among these of 11 cases had macular holes and posterior pole part holes,another of 7 cases had many holes.Another group had 11 cases,mean age was 45.8 years.These patients with inferior complex rhegmatogenous retinal detachment with secondary proliferative vitreoretinopathy(PVR) were included.Besides one case of giant retinal tear with between 2 and 7 clock hours,among these of 7 cases had one or two scleral buckling procedure,or pars plana vitrectomy with oil endotamponade.But inferior recurrent retinal re-detachment appeared.Surgical techniques included pars plana vitrectomy,membrane peeling,heavy water application,endophotocoagulation,cryocoagulation,endotamponade et al.Results:1or 13-month follow up,mean 4.3-month.Densiron-68 removal with 25 cases was after operation of 2 months,and didn't remove of 4 cases.After Densiron-68 removal,majority showed a stable reattached retina without further interventions,while,in one or two patients,recurrent retinal re-detachment appeared during Densiron-68 removal.Some of patients had partly lens opacification or lens post-capsular opacification.Part of patients had ocular hypertension.Conclusion:With Densiron-68,high anatomical and functional success rates can be achieved with inferior complex rhegmatogenous retinal detachment.Heavy silicone oil tamponade improves inferior tamponade,and may be considered a new generation of intraocular tamponades.But there were a significant early emulsification.Despite adequate Densiron fills,emulsification necessitated its removal.

Nowadays,acute pancreatitis remains a clinical challenge with considerable morbidity and mortality.Acute pancreatitis is associated with systemic and metabolic derangements which may promote hypermetabolism and negative nitrogen balance due to negative energy balance.Therefore,optimal nutritional support intreatmentof acute pancreatitis has been a subject for decades.Originally,the concept of pancreatic rest by fasting was thought to improve outcome as early enteral nutrition was believed to aggravate inflammation or infection through pancreatic stimulation.At present,an increasing number of studieswere performed showing numerousbenefits of enteral nutrition.In this review,intestinal dysfunctionandroute,timing,type and amount of enteralnutritional support in acute pancreatitis will be discussed.

Objective To study the outcome of pregnancy with lupus nephritis. Methods The clinical data of 9 pregnancies with lupus nephritis were retrospectively malyzed. Results Hypertension,proteinuria,injured renal function and abnormal serological marker were complicated with nine patients with lupus nephritis in pregnancy. The outcome of pregnancy was as following:abortion 2 cases,stillbirth 1 case,premature delivery 4 cases,term delivery 2 cases. All neonate survived without malformation. There was no postpartum hemorrhage or death. Patients with active SLE had very high fetal mortality( 100% ). And SLE got worsen in 50% of them. However,fetal mortality rate in pa-tients with controlled or quiescent SLE was 11.1% ,which was much lower than it in other patients(P<0.05). Con-clusion Lupus nephritis should be controlled before pregnancy. Pregnancy outcome could be improved by careful multidisciplinary monitoring and proper usage of adrenal cortical hormone.

Age-related macular degeneration(AMD) is the major cause of irreversible damage to vision in over 60 years old.Its incidence is increasing with age.Dry AMD is one of the most common types,causing the loss of vision is very slow.At the late stage,the geographic atrophy will be found,in which central vision is severely lost.It is widely believed that chronic inflammantion injury,oxidative stress injury,lipofuscin,drusen and choroid ischemia are the major pathogenesis.Now,the treatment is major aiming to the pathogenesis.But some new therapies such as crocetin,curcumin,ciliary neurotrophic factor,nanoceria,human monoclonal antibody,photoreceptor and stem cell transplantation have some efficiency to dry AMD.This article reviews the research advances in therapy of dry AMD.

? AIM: To evaluate efficacy of Ranibizumab and Conbercept for wet age-related macular degeneration ( wAMD) .?METHODS: This was a retrospective case series study. Thirty patients ( 30 eyes ) with wAMD were enrolled to receive intravitreal injections of ranibizumab (0.5mg) on 3 consecutive monthly schedule and 28 patients (30 eyes) with wAMD were enrolled to receive intravitreal injections of conbercept ( 0.5mg ) on 3 consecutive monthly schedule.Best corrected visual acuity ( BCVA ), optic coherence tomography ( OCT ) measurement were compared at 1mo after injections.?RESULTS: One month after every injection, the BCVA increased while the central macular thickness ( CMT ) decreased compared with those before treatment in group A and B (P<0.05).BCVA and CMT changes between two groups were no statistically different (P>0.05).?CONCLUSION:Ranibizumab and conbercept therapy can control the prognosis of wAMD and improve the vision effectively. There is no statistical difference on the curative effect between two drugs for 3mo.

Wet age-related macular degeneration is the leading cause of the severe, irreversible vision loss in individuals over the age of 65 years. Anti-VEGF therapy has been shown to play a key role in the pathogenesis of wAMD, which is the front-line therapy admittedly. lt has no clear steady curative effect for some patients even if they accepted repeating Anti-VEGF therapy. For the purpose of visual acuity improved more, a steady flow of new therapy has emerged, such as function towards the same or different targets of antiangiogenesis, consolidating the effect by combination therapy, improving or simplifying the mode of administration, etc. This paper gives a brief review of the progress of anti-VEGF for the therapy in wet age-related macular degeneration.