OBJECTIVE: To determine the residual levels of 4 organic solvents(methanol,alcohol,ether and acetone) in the raw material of medicinal sodium ozagrel by capillary gas(GC) chromatography.METHODS: The determination was performed using hydrogen flame ionization detector,AB-CarboWax with nitrogen gas as carrier gas using temperature programming for the column temperature.The sample size was 1 ?L,the temperature of the detector was 280 ℃,the temperature for sample injection was 250 ℃,the flow rate of carrier gas was 1 mL?min-1,and the flow rate of the hydrogen gas was 40 mL?min-1.RESULTS: The linear ranges of methanol was 0.037 5～3 mg?mL-1,alcohol,ether and acetone were 0.062 5～5 mg?mL-1,respectively,their average recoveries ranged from 98.07% to 99.12% with RSD being 0.34%～0.65%.Their lowest detection limits were all less than 3.125 ?g?mL-1,which were undetected for all the samples.CONCLUSIONS: The method is simple,sensitive and accurate,and it can be used for the determination of the residual levels of organic solvents in the raw medicinal of sodium ozagrel.

The people-oriented view is a universal idea,which holds different manifestations in different domain,and as for medical field manifests as the principle of no injury.There are certain obstacles during the application of no injury principle,including the neglect of the psychological injury,arrogance of the science,and the absence of humanities and psychology knowledge.This paper provides some countermeasure to make a best application of the no injury principle,that is,to stress the patients′ psychological health,eliminate the tyranny idea over patients,and consummate medical knowledge structure.

Objective To analyse the treatment,clinical features and prognosis of chronic lymphocytic thyroiditis combined with thyroid cancer.Methods Retrospectively analysed 40 cases with chronic lymphocytic thyroiditis combined with thyroid cancer,admitted to the Department of General Surgery of the First Affiliated Hospital of Dalian Medical University from November 2002 to April 2011.All the 40 patients were female.Results Ultrasound diagnosis or suspected diagnosis of chronic lymphocytic thyroiditis was made in 2 patients.Eleven patients underwent CT examination,and 2 patients were found with thyroid adenoma,1 patient found with thyroid cancer and lymph node metastasis,3 patients with chronic lymphocytic thyroiditis.Forty patients underwent surgical treatment,pathological results of 39 patients showed chronic lymphocytic thyroiditis with thyroid papillary carcinoma,1 patient was medullary thyroid carcinoma combined with chronic lymphocytic thyroiditis.Forty patients were administrated levothyroxine tablets.Thirty-six patients were followed up,4 patients were lost,1 relapsed,the others were in stable condition.Conclusions Preoperative diagnosis is difficult for chronic lymphocytic thyoiditis combined with thyroid cancer,which needs through the thyroid antibody tests,imaging examinations and intraoperative,postoperative pathologic examination and other means of comprehensive application.The method of definitive diagnosis for CLT combined with TC is intraoperative,postoperative pathologic diagnosis.The main pathological type is papillary thyroid carcinoma,which small papillary carcinoma occupies a large proportion.Surgery is the main treatment for CLT combined with TC.

Surgical treatment has improved the prognosis of resectable pancreatic cancer considerably de-spite the generally aggressive behavior of its malignancy. Neoadjuvant therapy for pancreatic cancer has been shown effective in improving a survival benefit. Few prospective randomized controlled clinical trials (RCTs) on the use of neoadjuvant chemotherapy and chemoradiation has been done tp show the survival advantage of systemic chemotherapy (5-FU/FA or gemcitabine) following surgical resection. Up to now, there is no high-level evidence of any benefit deriving from neoadjuvant chemo therapy for pancreatic cancer. Well designed trials are needed to compare neoadjuvant chemotherapy with surgery to judge the value of neoadjuvant cherqo-therapy in multimodal treatment concepts of pancreatic cancer.

Thyroid carcinoma is the most frequent endocrine cancer,and most are found in women.Different pathologic types of thyroid carcinoma has different biologic behaviors,and onset is hidden,which results in difficults to diagnose the early thyroid carcinoma.With the development of the molecular biology,more researches are focus on thyroid carcinoma in recent years.This article reviews the tumor markers,oncogene and gene therapy of thyroid carcinoma.

Stem cells research has generated markedly increasing interest and liver stem cells have enormous potential for clinical applications.Based on the development of modern cell biology,molecular biology and genetics,the fundamental study and clinical application of liver stem cells have been greatly progressed.This paper reviews current development of liver stem cells for clinical application.

It has been a problem in clinical practice that recurrence and distant metastasis of patients with colorectal cancer undergoing surgery.Recently,pericolonic tumor deposits has attracted extensive attention of scholars,and they think pericolonic tumor deposits may be closely related with colorectal cancer postoperative recurrence and metastasis.This review aims to introduce concept of pericolonic tumor deposits,pathological characteristics and its important role in clinical staging and prognosis,so as to provide clinical doctors medical advice to set more reasonable treatment and achieve the purpose of reducing tumor recurrence or metastasis for patients with colorectal cancer.

Hilar cholangiocarcinoma is the most common types of cholangiocarcinoma,it is difficult to perform operation owing to its anatomic characterstics.Currently,radical resection is the most important prognostic factors of hilar cholangiocarcinoma.In addition,lymph node metastasis and biomarkers such as CA19-9,hepatocyte growth factor receptor,interleukin-8,and so on,are also closely related to the prognosis of hilar cholangiocarcinoma.This paper will review the relevant researches of China and abroad to summarize important prognostic factors of hilar cholangiocarcinoma above-mentioned.

To clarify the progress of the study on the quality of Chinese medicine dispensing granules,and to provide reference for its further development and application.Referring to the relevant literature about the quality control of Chinese medicine dispensing granules,the main quality control methods,the quality and efficacy of traditional pieces,and the quality of traditional Chinese medicine extracts were analyzed and summarized.At present,the main methods of quality control ofTCM dispensing granule are high performance liquid chromatography and mass spectrometry,infrared spectroscopy.Compared with the traditional decoction and extract of TCM dispensing granule and traditional Chinese medicine composition,quality and effect of each has advantages and disadvantages,it needs further study.This paper reviewed the progress of quality control of Chinese medicine dispensing granules in recent years,and provided a reference for further research and development.

Objective To study the distribution of the gap conjunction and connexin(Cx)26 and 32 in inner ear of guinea pig. Methods We investigated expression and distribution of gap conjunction and Cx 26 and Cx 32 in cochlea of guinea by immunohistochemistry, RT-PCR and electron microscopy. Results T The gap conjunction was easily seen between hair cells and supporting cells in cochlea of guinea pigs. Cx26 was widely distributed in the stria vascularis, the spiral ligament and between the supporting cells, and Cx32 appeared mainly among supporting cells, in the stria vascularris. Conclusion There exist lots of gap conjunctions in inner ear of guinea pig. Two kinds of connexins can be seen in guinea pig's cochlea, and the Cx 26 plays more important role than other Cx.