Objective To explore the differences of cultivation and differentiation between cortical neural stem cells(C-NSCs) and mesencephalic neural stem cells(M-NSCs). Methods We dissociated embryonic cortex and ventral mesencephalon respectively,and single cell suspensions were achieved by trypsin digestion and mechanical dissociation in sterile condition.These cells were planted into DMEM/F12 culture medium with B27,bFCF,and then were passaged by mechanical methods and induced to differentiation in DMEM/F12 medium with 10%FBS(without growth factors).For examining the features of C-NSCs and M-NSCs,reverse phasecontrast microscopy,immunocytochemical techniques were applied to detect nestin,NSE,GFAP antigens and the specific antigen(TH) of neurons differentiated from the two kinds of NSCs.We observed and compared the difference of the two kinds of NSCs. Results The NSCs existed both in cortex and ventral mesencephalon of embryonic mice,which expressed nestin and possessed the ability of self-renewing and multipotent, but the density of NSCs in cortex was higher than that in ventral mesencephalon.There were some TH positive cells differentiated from M-NSCs but none came from C-NSCs.Conclusion The proliferative activity of C-NSCs is higher than that of M-NSCs,but more number M-NSCs differentiated into dopaminergic neurons in serum medium.

Burns ; complications ; immunology ; therapy ; Humans ; Immune System Diseases ; etiology ; therapy ; Nutritional Support

Burns ; complications ; metabolism ; Humans ; Inflammation ; etiology ; metabolism ; prevention & control

Objective To investigate the expression pattern of Placental growth factor (PlGF) and regulatory effects of interleukin-6 (IL-6)on PlGF expression in the primary cultured trophoblast cells. Methods Cytotrophoblast cells werecollected by trypsin-collagenase digestion and Percoll gradient centrifugation for primary culture. Reverse transcriptase polymerase chain reaction(RT-PCR) were resorted to demonstrate the expression pattern of PlGF mRNA in trophoblast cells cultured in vitro. The effects of IL-6 with different concentration(100,10,1 and 0.1 ?g/L) and duration (6,12,24 and 48 h) on PlGF expression were observed. Results The 185 bp and 248 bp bands of PlGF were showed by RT-PCR. PlGF expression correlated with IL-6. PlGF began to increase at 6 h, and reached the climax at 12 h when recultured with 100 ?g/L IL-6. Conclusions PlGF expression has time and dose dependance on IL-6. It may play an important role in early pregnancy.

Objective To study the growth inhibitory effect of chemotherapeutic drugs on the pancreatic cancer cell line Aspc-1 and Bxpc-3, and the relations to the varied concentration and exposere time of chemotherapeutic drugs administrated in single or in combination. Methods Four chemotherapeutic drugs included 5-fluorouracil (5-FU), epirubicin (E-ADM), mitomycin(MMC) or cisplatin (DDP) administrating in single or in combination and using varied concentrations(d1、d2、d3 and d4) and exposure times(24、28 and 72 hours); and then the growth inhibitory effect on the two cell lines. resulted from these drugs were assayed by MTT colorimetry and analysed by “ t ” test. Results The growth inhibitory rate of two cell lines was increased, with the increment of drug concentration and/or the prolongation of exposure time ( P

Objective To explore the risk factors of lung cancer in rural area. Methods A 1∶1 matched case_control study was conducted in 106 individuals in Heilongjiang province. Results The amount of passive smoking (OR:2.48,95%CI:1.51～4.08),history of mental scar (OR:4.63,95%CI:1.51～14.15), smoking from Kang (OR:1.69,95%CI:1.10～2.59), smoking indexes (OR:1.75,95%CI:1.10～2.79) and chronic bronchitis (OR:4.67,95%CI:1.12～19.49) had a closely correlation with lung cancer in rural area.Conclusions Lung cancer might be caused by multiple factors synergetically.The main countermeasures for controlling lung cancer were to give up smoking and to improve the conditions of heatable brick bed and heating method.

Objective To make a nationwide external quality assessment for drug sensitivity testing of Neisseria gonorrhoeae, analyze the problems in and factors associated with the drug sensitivity testing, and to enhance the quality of drug sensitivity testing of N. gonorrhoeae at different monitoring sites. Methods Samples were uniformly delivered to monitoring sites by express mail service. Test results were analyzed in the National Center for STD Control, and the evaluation results were fed back to these monitoring sites. Results A total of 105 quality control samples were delivered from 2007 to 2009, with a response rate of 88.57% (93/105). Thirteen monitoring sites were enrolled in the external quality assessment, including 9 laboratories in 2007, 9 in 2008 and 13 in 2009. The total percentage amounted to 77.42% (24/31) for qualified laboratories during the 3 years, including 6 laboratories in 2007, 7 in 2008 and 11 in 2009. The coincidence rate increased for the detection of penicillinase-producing N. gonorrhoeae (PPNG), N. gonorrhoeae with chromosome-mediated ciprofloxacin resistance, and N. gonorrhoeae with chromosome-mediated spectinomycin resistance, and declined for the detection of N. gonorrhoeae with plasmid-mediated high level tetracycline-resistance (TRNG) and N. gonorrhoeae with chromosome-mediated ceftriaxone resistance. Conclusions The 3-year external quality assessment reveals an improvement in the overall quality of drug sensitivity testing of N. gonorrhoeae at national monitoring sites; the accuracy is improved markedly for the detection of PPNG, N. gonorrhoeae with resistance to spectinomycin and ciprofloxacin, but is needed to increase for the detection of ceftriaxone-resis- tant N. gonorrhoeae and TRNG.

Objective To investigate the therapeutic time window and the dose response effects of epirubicin on the expression of c-FLIP in breast cancer.Methods MCF-7and MDA-MB-231 breast cancer cells were divided into two groups: epirubicin groups were treated with 4.0,2.0,1.0,0.5 and 0.25mg/L of epirubicin,and control groups were treated with 0.9% sodium chloride solution at the same dose.After treatment for 24,48 and 72 h,the incubated cells were collected for the measurement of c-FLIP by RT-PCR,and for examination of percent of apoptosis cells with flow cytometry.ResultsA dose-time-dependent pattern was observed.The expression of c-FLIP in MCF-7and MDA-MB-231 breast cancer cell lines declined gradually as the epirubicin concentration increased and treatment time was prolonged.Percentage of apoptosis breast cancer cells increased gradually as the epirubicin concentration was increased and treatment time was prolonged,and percentage of apoptosis cells was the highest when breast cancer cells were treated with 2 mg/L epirubicin for 72 h.ConclusionsEpirubicin can promote apoptosis of breast cancer cells by inhibiting the expression of c-FLIP,and its inhibitory effect is most pronounced when breast cancer cells are treated with 2 mg/L epirubicin for 72 h.

OBJECTIVE:To establish a method for the simultaneous determination of chloramphenicol and dexamethasone sodium phosphate in ear drops by HPLC METHODS:Chloramphenicol and dexamethasone sodium phosphate were separated with a mobile phase of methanol-phosphate buffer solution(0 34% KH2PO4 solution)(60∶40,v/v,1 0ml/min)on Kromasil C8 column and detected at 240nm RESULTS:Good linearity appeared in the concentration of chloramphenicol 100～500?g/ml,r=0 9 999,the recovery was 98 5%～100 8%,RSD≤1 5%;and good linearity showed in the concentration of dexamethasone sodium phosphate 16～80?g/ml,r=0 9 990,the recovery was 98 8%～100 6%,RSD≤2 0% CONCLUSION:This method can simultaneously detect both ingredients in the ear drops