BACKGROUND: Studies have shown that Maca can enhance immune cel function, improve mitochondrial respiratory chain enzymes, and play a role in anti-oxidation and anti-aging.
OBJECTIVE: To observe Maca effects on kidney mitochondrial respiratory function and anti-aging capabilities in elderly rats after exhausted exercise.
METHODS: 10-month-old elderly rats were intragastrical y administrated Maca powder 5 g/kg, once a day, and did treadmil exercise, 5 days in a week. Rats were randomly divided into control group, Maca group and training group and Maca+training group. At 6 weeks after training, rats in each group did exhausted exercise (35 m/min), and immediately received intraperitoneal injection of 2% sodium pentobarbital. The kidney was obtained, and mitochondria were extracted by differential centrifugation. A spectrophotometer was used to measure mitochondrial respiratory chain activity.
RESULTS AND CONCLUSION: (1) Mitochondrial respiratory chain enzyme and antioxidant enzyme activity: mitochondrial respiratory chain enzyme I-III, superoxide dismutase and glutathione peroxidase activities were higher in the Maca+training group than in the control group, Maca group and training group (P < 0.05 or P < 0.01), but malondialdehyde content was lower (P < 0.05 or P < 0.01). (2) Results suggested that the combination of supplement Maca and incremental exercise can improve mitochondrial respiratory function and delay aging in the kidney of aged rats after exhausted exercise. Moreover, supplemented Maca and incremental exercise have a synergistic effect.

Objective To compare the effects of vasopressin and adrenaline on cerebral resuscitation in rats. Method Sixty male SD rats were used to set the animal model of cardiac arrest and were randomly (random number) divided into 4 groups (n = 15/group): sham operation group (sham-gro), negative control group (neg-gro), vasopressin group (vas-gro) and adrenaline group (adr-gro). Blood pressure was recorded, and brain tissue samples were obtained. Results There was no significant difference in the recovery rate between vas-gro and adr-gro 30 min after resuscitation (P ＜0.05). The mean blood pressure in vasgro was higher than that in adr-gro within 30 min (1,2, 5, 10, 20, 30 min) after restoration of spontaneous circulation. The measured MDA, SOD and GSH were significantly different (P ＜ 0.05) between each two groups among vas-gro, sham-gro and adr-gro. Conclusions The recovery rate was similar between vasopressin group and adrenaline group during cardiopulmonary resuscitation in rats. However, vasopressin can to maintain the mean arterial pressure at a higher level after resuscitation, which can the increase the cerebral perfusion and reduce brain cell damage.

Reviewing the theory of chaos in the field of nonlinear dynamics, this paper summarized theory of chaos and its applications in medical signal processing including electroencephalogram(EEG) signal?electrocardiogram(ECG) signal?electromyogram(EMG) signal, etc. The foreground is reviewed to advance the study and application of nonlinear dynamics theory in biomedical engineering.

The characteristics of medical and health literature novel assessment in institutions of armed forces were described from the secrecy of projects for literature novelty assessment , the limitations of consignee institution , and the non-profitability of literature novelty assessment agency .The major problems of medical and health literature novel assessment in institutions of armed forces were elaborated in terms of the literacy, instability, competency and knowledge limitation of literature novel assessment staff with suggestions put forward for their solution such as strengthening the construction of talent team, joint development and sharing of resources.

Objective To explore the effects of estrogen,sport walking with weight and estrogen plus sport walk on serum lipid,bone metabolism and bone mineral density(BMD) in postmenopausal women.Methods 52 postmenopausal women were divided into the hormone replacement therapy(HRT) group(n=12),sport walking with weight group(Sp group,n=14),HRT + Sp group(n=12) and control group(n=14).HRT group took compound nylestriol,Sp group took sport walking with 5 kg weight and exercise intensity was from 60% to 80% VO2max,HRT+Sp group received two treatments as HRT group and Sp group.The experimental duration consisted of six months.Results The total cholesterol(TC),TC/high density lipoprotein cholesterol(HDL-C),alkaline phosphatase(APL) and urine calcium(Ca)/creatinine(Cr) were markedly lower in three experimental groups than the control group and before experiment(P<0.05 or P<0.01),HDL-C in Sp and HRT+Sp groups were markedly higher than the control group and before experiment(P<0.05),BMD of spine(L2～L4) and left trochanter in three experimental groups were markedly higher than the control group(P<0.01) and before experiment(P<0.0).Conclusion Both estrogen replacement therapy and sport walking with weight effectively improve serum lipid,prevent and reverse osteoporosis induced by menopause in women.

Sepsis is a life-threatening organ dysfunction caused by dysregulated immune response to infection, which has high rates of morbidity and mortality.The imbalance between the response to inflammation and that to anti-inflammation is one of the most important pathophysiological processes in patients with sepsis.Despite the variety of its treatment options, their therapeutic effects remain unsatisfactory.Continuous renal replacement therapy (CRRT) is gaining a wider application in critical care medicine, especially in the treatment of sepsis, which can significantly increase the survival rate of sepsis patients by immunity modulation, organ support, and internal environment stabilization, so as to improve the immune disorder, protect the organ functions, and maintain homeostasis in the body.

In the past decades, the advent of molecular targeted agents has been a considerable breakthrough for cancer patients, ex-cept for traditional operation, chemotherapy, and radiotherapy. As a novel therapeutic method, molecular targeting treatment is im-portant in the field of medical oncology, in which epidermal growth factor receptor inhibitors (EGFRIs) have been widely used as target-ed agents. Dermatological toxicities are the common side effects associated with the EGFRIs. Diarrhea, weakness, and pneumonia can be observed. EGFRI-induced dermatological toxicities may disrupt the health-related quality of life and cause anticancer treatment dose adjustments or discontinuance. The dermatological toxicities mainly involve rash acneiform, pruritus, xerosis, paronychia, hair change, and skin hypersensitivity. Treatment recommendations from guidelines include antibiotics, corticosteroids, and antihista-mines, but their clinical therapeutic efficacy have not been proven. Therefore, oncologists and dermatologists are investigating effec-tive medication. This article reviews the advances in the study of the clinical manifestations and drug therapies of EGFRI-induced der-matological toxicities to provide reference for clinical practitioners.

Objective:LAPTM4B is a novel gene which is over expressed in HCC.To evaluate LAPTM4B gene promoter on gene transcription,we construct luciferase reporter plasmid pGL3-LAPTM4B-Luc.Methods:The LAPTM4B cDNA was used as a template in the polymerase chain reaction to amplify its upstream regions from the translation initiation codon.The PCR product was directly cloned into the luciferase reporter vector pGL3-Basic.Results:Restriction enzymes digestion and nucleotide sequencing confirmed that the recombinant plasmids were correct without base mutation and deletion.Conclusion:A known gene promoter sequence could be freely obtained from Genbank database.This is very useful for the gene promoter cloning and promoter vector constructing.The construction of LAPTM4B recombinant plasmid lay a foundation of the analysis of promoter activities.

Objective To investigate the therapeutic effect and side effect of combined capecitabine with oxaliplatin on advanced and recurrent gastric cancer.Methods 56 patients with advanced and recurrent gastric cancer were treated with combined oxaliplatin and capecitabine chemotherapy,and the therapeutic effect and side effect were analyzed retrospectively.Results Among all 56 patients treated with combined oxaliplatin and capecitabine chemotherapy,4 patients (7.1%) were complete remission,26 patients (46.4 %)were partial remission,20 patients (35.7 %) were stabilization,6 patients (10.7 %) were progression,and the response rate was 53.6 %.The median remission time was 7.8 months,and the median survival time was 11.3 months.The main side effects were nausea and vomiting,diarrhea,hand-foot syndrome,peripheral nerve toxicity,transaminase increasing and leuco cytopenia,however,the side effects were mainly grade Ⅰ and Ⅱwhich could be tolerated by patients.Conclusion The combined oxaliplatin and capecitabine chemotherapy is an effective method and has better tolerance in treatment of advanced gastric cancer and is well worth clinical application.

Objective:To explore the factors influencing the medical service in a Chinese hospital during the period of management transformation,and to study the changes of these factors at different phases of hospital development.Methods: Data were collected from Hospital Information System(HIS) at the end of every month from Jan.2000 to Dec.2003 and 8 variables were deduced: the monthly outpatient visits and emergency visits,the monthly inpatient number,the monthly person-time of operation,bed utilization rate,the monthly cycle of bed rotation,ALS(average length of stay),proportion of cured or recovered patients and expenditure of hospitalization per person.Factor analysis was used to search for the common factors.And Kruskal-Wallis H test was used to compare the mean factor score at different phases divided by ordinal clustering method.Results: There were 2 common factors: quantity-profit factor(F_(1)) and quality-efficiency factor(F_(2)),influencing the medical service in the hospital.F_(1) score increased with the pregression of 4 stages(preliminary,initial,developmental and stable phases) of hospital management transformation and F_(2) score remained unchanged.Conclusion: The quantity-profit of this hospital increases and the quality-efficiency remains unchanged during the 4-year management transformation.Different common factors have different developmental tendency.