Objective To detect the effect of analgesic labor with inhaling nitrous oxide gas for the duration of labor and mother and fetus. Methods Choose 220 cases of normal primipara, 120 cases were observed group, the primipara were inhaled the mixture gas(50% nitrous oxide and 50% oxygen) when the duration of labor entered the progress from active phase to the cervical dilatation were all opened, 100 cases were control group. The effect of anal-gesia, the change of breath and postpartum hemorrhage, neonatal scores were observed. Results The observed group has good effect on anlgesia, the effective rate is 93.3 % . The active phase were obviously shortened and has bad effect to mother and fetus. Conclusion It was safety and effective by inhaling nitrous oxide gas for analgesic labor and worthy to generalized and applied.

Objective To determine whether treatment with Gliclazide (Diamicron) is able to prevent development of microvascular complications in type 2 diabetes mellitus. Methods This trial was carried out in seven centers of the National Diabetes Mellitus Collaborative Study Group. Two hundred and eighty five patients with type 2 diabetes mellitus were recruited from six areas of China and divided into Gliclazide group (n=155) and Glibenclamide group (n=130). Age, sex, duration of diabetes and body mass index were recorded at entry. Fasting and 2h postprandial blood glucose were measured monthly while HbA 1c was determined at 3 month interval. Retinal photography and fluorescein angiography were performed in the 1st and 3rd year.Results There were no significant differences in age, sex, blood pressure, duration of diabetes, mean blood glucose and HbA 1c between the Gliclazide and Glibenclamide groups at baseline, in the 2nd and 3rd follow up year. There were no significant differences in retinopathy between the two groups at baseline, but at the end of the 3rd year, the subjects in Gliclazide group with retinopathy progression over one grade were significantly less than those in Glibenclamide group (2.58% vs 18.46%, P

Objective To investigate the influence of gene transfection antiangiogenesis on microvessel and relative cytokine of hypertrophic scar of rabbits' ear.Methods The hypertrophic scar of rabbtis' ear was reproduced.On the 10th day after epithelization,Ad-METH-1 was injected into tissue of scar.30 days later,the microvessel of scar-tissue was detected by microcirculation microscope.Meanwhile.H&E and immunohistochemical stains were performed.Then the results were analyzed.Results 30 days after Ad-METH-1 injection.in experimental groups,the microvascular count of scar tissue was 12.38±2.56,the percentage of VEGF positive cells was 17.64%,and the percentage of bFGF positive cell was 18.24%:while in the control groups,the microvascular count of scar tissue was 48.12±6.46.the percentage of VEGF positive cell was 31.34%.and the percentage of bFGF positive eell was 28.26%.Results revealed that the count of microvessel of scar tissue in the experimental groups was lower than that in the control groups,between which there was the difference in statistics(P＜0.01).and that the percentage of VEGF and bFGF positive cells of scar tissue in the expenmental groups were lower than that in the control groups.between which there was the difference in statistics(P＜0.05).Conclusion Ad-METH-1 has marked inhibitory effects on scar tissue hyperplasia of rabbits' ear,angiogenesis and expression of VEGF and bFGF.Using antiangiogenesis therapy at the early phase could inhibit the formation of hypertrophic scar.Gene transfection antiangiogenesis therapy could bid fair to become an effective method to prevent and treat hypertrophic scar.

Objective To investigate the effect of total flavones of Rhizoma Drynariae on femur distraction osteogenesis in the rabbits. Methods Thirty-two healthy rabbits were randomly divided into treatment group and control group, 16 rabbits in each group. The femoral fracture was treated with unilateral femoral distraction and was fixed with a self-made distraction instrument. After 7-day intermittent period, the fractured femur was distracted at a rate of 1 mm/d, twice a day for 10 continuous days. The treatment group was fed with total flavones of Rhizoma Drynariae from the first post-operative day to the end of the experiment. And then all of the animals were sacrificed after fixation for 28 days. The bone specimens were used for histological observation and immunohistochemical detection. Results The area of mature bone in the newborn bone tissue of the treatment group was increased, and osteoblasts number and the percentage of trabecular bone area were significantly higher than those of the control group . The bone morphogenetic protein-2 (BMP-2) and transforming growth factor-β1 (TGF-β1) were stained brown deeply, the staining degree being stronger than that of the control group. Conclusion Rhizoma Drynariae total flavones can effectively accelerate the formation and maturation of newborn bone tissue during bone distraction.

Objective:To investigate the possible role of NKT cells in unexplained recurrent spontaneous abortion by measuring the NKT cell numbers,maturity and cytokine secretion of the placenta of mice with unexplained recurrent spontaneous abortion.Methods:Normal pregnancy model in hybrid by feeding CBA / J and BABL/C in a cage,and the model of unexplained recurrent spontaneous abortion was established by feeding CBA / J and DBA2/J in a cage.The number of NKT and CD3~+T cells was determined by flow-cytometry;Th1/Th2-relative cytokines were assayed by ELISA and T-bet expression was determinded by fluorescence quantitative PCR.Results:There was not significant change of CD3~+ T cells when compared between normal pregnancy and unexplained recurrent spontaneous abortion group (P＞0.05).In the course of normal pregnancy,the IFN-γ secreted by placenta lymphocytes decreased gradually,accompanied by the decline of NKT cell number and the proportion of mature cells;whereas in the course of unexplained recurrent spontaneous abortion,it was on the opposite.There was significant difference of T-bet mRNA expression between the two groups.T-bet mRNA expression was related to the proportion of mature NKT cells or placenta IFN-γ secretion by lymphocytes.Conclusion:Unexplained recurrent spontaneous abortion may be related to NKT cells disorders,NKT cells are of low-mature proportion and inadequate secretion of IFN-γ during early pregnancy,whereas are shown high-mature proportion and excessive secretion of IFN-γ during latter pregnancy;the anomaly of T-bet mRNA expression may be one of the factors leading to NKT cells disorder.

Objective To understand the prevalence state of drinking-tea type fluorosis in Hainan Prefecture,Qinghai Province,and provide scientific data for its prevention and control.Methods In 2016,a cross-sectional survey method was used to select five counties of Guide,Guinan,Xinghai,Gonghe and Tongde in Hainan according to "National Investigation Measure of Prevalence of Drinking-tea Type Fluorosis".In each county,5 villages (towns) were selected according to the directions of east,west,north,south and center positions,and 1 administrative village was randomly selected in each village (town) as the survey site.All the children aged 8 to 12 years old at the survey site and 60 adults aged 16 years old and older at each survey site were selected as the survey subjects.We investigated the volume of brick-tea water consumed per day among objective population with questionnaire,and collected water,brick-tea water and urine samples (if each region has one water source,we collected one water sample,if this place have different water sources,we collected all the water samples from each water source;brick-tea water samples were collected based on different locations;more than 12 urine samples were collected in each survey site).The fluoride concentration in water,urine and tea water were detected with fluoride ion-selective electrode,the dental fluorosis of children aged 8-12 years were diagnosed by "Diagnosis of Dental Fluorosis" (WS/T 208-2011),and clinic skeletal fluorosis of population was diagnosed based on the "Diagnostic Criteria for Endemic Skeletal Fluorosis" (WS 192-2008).Results The medians of fluoride in drinking water,drinking-tea water were 0.29 and 1.43 mg/L,respectively.The mean of daily fluoride intake of each person was 1.82 mg,and the geometric mean of urinary fluoride was 0.83 mg/L;the rate of dental fluorosis among children was 14.06％ (559/3 975),its index was 0.24,the prevalence rate of clinic skeletal fluorosis among adult was 8.01％ (119/1 484).The rate of dental fluorosis among children in Tongde County was the highest,about 15.96％ (83/520),while the rate of dental fluorosis among children in Guinan County was the lowest,about 10.76％ (111/1 032).The prevalence rate of clinic skeletal fluorosis among adult in Xinghai County was the highest,about 10.87％ (30/276),while Tongde County was the lowest,about 5.75％ (15/261).Conclusions The prevalence of drinking-tea type fluorosis is relative low.However,the local population are still under the risk of brick-tea with high fluoride,so it is necessary to strengthen the health education propaganda to keep the population healthy.

Objective To analyze the radiographic characteristics of right hand X-ray film of adult patients with Kaschin-Beck disease (KBD) in Qinghai Province, to understand the most affected locations in adult KBD. Methods According to the criteria of KBD diagnose (WS/T 207-2010), 111 cases of patients were taken X-ray films of right hands. Joint space narrow, joint deformity, subchondral sclerosis, osteophyte, coarse and irregularity of joint, marginal retraction sign and capsule changes were chosen as the descriptive indexes, and these indexes were analyzed with SPSS 17.0. Results A total of 111 cases adult patients with KBD were examined right hand by X-ray, abnormality on X-ray film were 103 cases, the abnormal rate was 92.79%. The most affected fingers were Ⅱ- Ⅳphalanx bones, Ⅱphalanx bones accounted for about 92.23% (95/103), Ⅲ phalanx bones accounted for about 99.03% (102/103), and Ⅳ phalanx bones accounted for about 99.03% (102/103). There was significant difference of the abnormality between th e proximal phalanx and the middle phalanx among the Ⅱ - Ⅳ phalanx bones(χ2=79.33, P<0.05). Abnormal numbers of joint deformity, marginal retraction sign, coarse and irregularity of joint, osteophyte, capsule changes and joint space narrow in the proximal phalanx were 212, 7, 134, 47, 15 and 115 in Ⅱ - Ⅳ proximal phalanx, respectively; while the abnormal numbers of joint deformity, marginal retraction sign, coarse and irregularity of joint, osteophyte, capsule changes and joint space narrow in the middle phalanx were 77, 37, 137, 26, 19 and 126 in Ⅱ - Ⅳmiddle phalanx, respectively. Conclusion The Ⅱ - Ⅳ phalanx bones of right hand are the most affected locations in adult KBD.

Objective To understand the epidemiological prevalence of drinking-tea type fluorosis in Haidong Region,Qinghai Province,and provide scientific data for its prevention and control.Methods According to the investigation plan of drinking-tea type fluorosis in China,cross sectional survey was adopted to select 6 counties in Haidong Prefecture from May to July in 2016.Five townships were selected according to the eastern,western,north and south directions,1 administrative village was randomly selected in each township (town) as the survey site.Water and brick tea samples were collected to test fluoride ion concentration;dental fluorosis in children aged 8 to 12 years and adult skeletal fluorosis were examined.Fluorine ion selective electrode was used to determine fluoridated fluoride (WS/T 106-1999),fluorine in urine (WS/T 30-1996) and fluorine content in brick tea (GB 19965-2005).Dental fluorosis was examined by Deans method.The diagnosis of skeletal fluorosis was referred to the "Diagnostic Criteria for Endemic skeletal Fluorosis" (WS 192-2008).Results Fifty-seven drinking-water and 56 brick-tea samples were determined,the means of fluoride in drinking water and brick-tea were 0.25 mg/L and 724.71 mg/kg [which was significantly higher than the national standard (300 mg / kg)],respectively.Totally 1 207 urinary samples of children and 1 428 urinary samples of adults were determined,the geometric means of urinary fluoride of children and adults were 0.57 and 0.62 mg/L,respectively;2 677 children and 1 968 adults were examined,the rate of dental fluorosis among children was 15.09％ (404/2 677),its index was 0.26,the prevalence rate of clinic skeletal fluorosis among adults was 2.44％ (48/1 968).Conclusion The fluoride content of brick tea in 6 counties of Haidong Prefecture of Qinghai is higher than that of the national standard.Local residents are still exposed to high fluoride brick tea,and health education and publicity should continue to be strengthened.

Objective To observe the illness change trend of drinking water type endemic arsenic poisoning in Qinghai Province, comprehensively evaluate the effect of prevention and control measures, in order to provide a scientific basis for timely adjustment of the prevention and control measures. Methods From 2010 to 2013, according to the Drinking Water Type Endemic Arsenic Poisoning Monitoring Programs, 3 villages in 2 counties within Qinghai Province were randomly selected as monitoring sites. The arsenic content in drinking water of residents was measured, water improvement projects in all monitoring villages were investigated; at the same time an investigation of arsenic disease in resident population was conducted, and urinary arsenic content was monitored. Arsenic in drinking water and urine was determined by hydride generation atomic fluorescence spectrometry, and arsenic poisoning was diagnosed using Endemic Arsenic Poisoning Diagnostic Criteria (WS/T 211-2001). Results Of the three water improvement projects, two were water arsenic exceeded the standard, and one was intermittently operated. From 2010 to 2013, the arsenic poisoning detec tion rate in Baoning Village was 27.30% (193/707), 31.90%(245/768), 29.35%(221/753) and 28.22%(219/776); in Kecai Village was 32.62%(107/328), 34.83%(124/356), 31.26%(131/419) and 29.35%(118/402);and in Manimotai Village was 56.58%(43/76), 52.81%(47/89), 45.10%(46/102) and 34.69% (34/98), there was no significant difference statistically of the arsenic poisoning detection rates in the three monitored villages in the 4 years (χ2 =3.09, 0.04, 0.92, all P>0.05). From 2010 to 2013, women arsenic poisoning detection rate was 36.45%(203/557), 40.59%(246/606), 36.12%(225/623) and 34.77%(218/627), respectively;men was 25.27%(140/554), 28.01%(170/607), 26.57%(173/651) and 23.57%(153/649), respectively;women arsenic poisoning detection rates were higher than those of men (χ2 = 16.25, 21.32, 13.49, 19.38, all P < 0.05). Arsenic poisoning detection rate of people younger than 60 years old had a tendency to increase with age. In 2012 and 2013, 105 and 93 urine samples were tested, respectively; urinary arsenic geometric mean was 0.113 and 0.149 mg/L. Conclusions Water improvement projects and water quality are not optimistic, and prevalence of arsenic poisoning is still at a higher level. A sound long-term monitoring program should be established as soon as possible, the management and maintenance of water improvement projects should be strengthened, and the monitoring and prevention work should not be neglected.

Objective: To investigates the role of piwi-interacting RNAs (piRNA) in the occurrence and development of hepatocellular carcinoma. Methods: Second-generation small RNA sequencing was performed on cancer and paracancerous tissues, metastatic and non-metastatic liver cancer tissues of patients with liver cancer, and the sequencing data were filtered out for the common RNA sequences to be repeated. The piRNA predictor was used to forecast the possible new piRNA merged with the downloaded known piRNA to screen out distinction. A miRanda algorithm was used to predict the corresponding target genes and functional enrichment analysis. piRNA was selected for experimental functional (migration) analysis. An independent t-test was used to compare means between the two groups. Results: 66 772 piRNAs (known 149) were obtained by sequencing. 241 piRNAs were found in cancer and paracancerous tissues, and 1 634 piRNAs were found in metastatic and non-metastatic tumors. Analysis of the GO and KEGG pathways of the target genes of differential piRNAs revealed that they were mainly involved in cell adhesion. An experimental functional analysis was performed on the selected Pirna (PIR1/97), which showed that it promoted the migration of hepatoma cells (LM3: t = 8.829, P < 0.05; PLC: t = 7.318, P < 0.05). Conclusion The expression levels of piRNA in hepatocellular carcinoma patients with cancer and paracancerous tissues, metastasis and non-metastatic liver cancer tissues are different and it could be entailed in the metastasis process of hepatocellular carcinoma. Hence, experimental functional analysis is required for research and experimental confirmation.