ObjectiveTo detect the effect of LMWH on Oligohydramnios at mid and late trimester of pregnancy.MethodsPatients were divided into two groups.Control group was given Compound amino acids 500ml,5％glucose solution 500ml by intravenous infusion and water 1000ml per oral per day,and rest in bed with left lateral position for 10 days.Test group was given the same treatments as control group,plus Flagmin 5000U by subcutaneous injection per day.Amniotic fluid index was retest after treatment.Delivery mode,newborn birth weight,neonatal asphyxia and amount of postpartum hemorrhage were compared between two groups.ResultsAmniotic fluid index and newborn birth weight of test group was significantly higher than control group( P ＜ 0.05).Cesarean section rate,mild neonatal asphyxia rate was significantly lower than control group( P ＜ 0.05).Severe neonatal asphyxia rate and amount of postpartum hemorrhage had no significant differences (all P＞0.05).ConclusionLMWH treatment could increase amniotic fluid volume and newborn birth weight on Oligohydramnios at mid and late trimester of pregnancy,improve pregnancy outcome,without increase of postpartum hemorrhage.

Objective To explore the anomalous effects of India buead and coix seed powder on phlegm-dampness constitution dyslipidemia through population-based intervention studies. Methods According to the experimental results of the model group with dyslipidemia, the phlegm-dampness population meeting the inclusion criteria were chosen as the samples for the randomized controlled trial, who were divided into test meal (21 males and 39 females) and blank control (23 males and 37 females) groups with 60 cases in each, aged 18 to 65 y and the total of 120 cases completed the study. Statistical analyses were performed with SPSS 18.0 statistical software. The self comparison wass conducted by the method of paired-sample t test and the comparison between groups was conducted by using independent sample t test. The categorical statistics were described as frequency and compared throughc2 test, P<0.05 was considered as statistically significant. Without changing the original way of life, the meal group having taken the India buead and coix seed powder was compared independently and with the control group for TC, TG, HDL-C and LDL-C to detect the trends and degrees of the level changes. Meanwhile, according to efficacy tests and standards, the changes in the level of TC, TG and HDL-C have been evaluated and the efficiency and total effective rates of all the variables have been calculated. Results After the intervention, there was a declining tendency in each variable, 8.9%in TC, 21.4%in TG, and 27.2%in LDL-C, except for an increase of 0.13 mmol/L in HDL. There were significant differences in the variables before and after the intervention (P<0.05 in each). As for the test meal group after the intervention, the effectiveness of TC has been obtained in 25 cases and inefficacy in 35 cases, with efficiency of 41.7%;the effectiveness of TG has been shown in 53 cases and inefficacy in 7 cases, with efficiency of 88.3%;the effectiveness of HDL-C has been gained in26 cases and inefficacy in 34 cases, with efficiency of 43.3%; and the total effective rate was 57.8%. Conclusions There are significant changes and improvements in TC, TG, HDL-C and LDL-C in the test meal group after the intervention with the powder, which demonstrate that the powder is effective in the reduction of TG for animals with dyslipidemia and populations with phlegm-dampness constitution.

Background Electroacupuncture pretreatment plays a protective role in myocardial ischemia/reperfusion (I/R) injury and microRNAs (miRNAs) could act on various facets of cardiac function. However, the role of miRNAs in the cardioprotection by electroacupuncture pre-treatment on myocardial I/R injury remains unknown. The purpose of the study was to examine whether miR-214 was involved in cardio-protection by electroacupuncture. Methods Using rat myocardial I/R model, we examined the role of electroacupuncture pretreatment in myocardial I/R injury and analyzed the changes in the expression of miR-214. In addition, I/R was simulated in vitro by performing oxy-gen-glucose deprivation (OGD) on H9c2 cell cultures, and the effect of electroacupuncture pretreatment on I/R injury as well as expressional level of miR-214 were examined in vitro. Furthermore, the miR-214 mimic was transfected into OGD-treated H9c2 cells, we analyzed the cell apoptosis, lactate dehydrogenase (LDH) and creatine kinase (CK) activities, intracellular free Ca2+concentration ([Ca2+]i) as well as the relative protein levels of sodium/calcium exchanger 1(NCX1), BCL2-like 11 (BIM), calmodulin-dependent protein kinase IIδ(CaMKIIδ) and Cyclophilin D (CypD). Results The in vivo results revealed that compared with the I/R group, the electroacupuncture pretreatment group showed significant decreased myocardial infarct size, as well as the increased indices of the cardiac function, including heart rate, mean arterial pressure, left ventricular systolic pressure and maximal rate for left ventricular pressure rising and declining (±dp/dt max). In addition, electroacupuncture pretreatment could inhibit the elevation of LDH and CK activities induced by I/R injury. The quantitative PCR (qPCR) results demonstrated electroacupuncture pretreatment could provide cardioprotection against myocardial I/R injury in rats with miR-214 up-regulation. In the meanwhile, in vitro, electroacupuncture pretreatment protected H9c2 cells from OGD-induced injury. Trans-fection of miR-214 mimic showed protective effects on OGD-induced injury to H9c2 cells by reducing apoptosis, decreasing LDH and CK activities, rescuing the OGD-induced Ca2+and down-regulating elevated protein levels of NCX1, BIM, CaMKIIδand CypD. Conclusions Our findings firstly demonstrated that electroacupuncture pretreatment promotes the expression of miR-214 in myocardial I/R injury and miR-214 contributes to the protective effect of electroacupuncture on myocardial I/R injury.

Objective To study the clinical effect of low molecular weight heparin on fetal growth restriction (FGR) prevention in early pregnancy.Methods 100 pregnant women had been employed in our study,they were divided into two groups,observation( n =60) and control( n =40) group.Both groups were given the natural vitamin E 0.1g,and folie acid 0.4mg daily oral administration,continuing to 12 weeks of gestation.5000 u of subcutaneous dalteparin sodium was added to pregnant women with notmal level of D-dimer in the observation group until 12 weeks of pregnancy,while the dose of Fragmin was adjusted in pregnant women with abnormal level of D-dimer until retuned to normal level.The pregnacy outcome of two groups were compared.Results The gestational age,birth weight,placental weight of the obseruation group was signiticantly higher than thal of the control group ( t =4.55,2.79,11.91,all P ＜ 0.05 ),while FGR,oligohydramnies,fetal distress and the incidence of hypertensive disorters of the obseruation group were significantly lower than that of control group ( x2 =6.50,20.55,7.87,3.76,all P ＜ 0.05 ) ; Compared to the control group,the observation group didn't have higher incidence of intrauterine fetal death,neonatal asphyxia and perinatal death ( P ＞ 0.05).Conclusion Low molecular weight heparin using in early pregnancy was effective in preventing FGR in pregnant women with FGR risk factors.

The roots of Glycyrrhiza uralensis are widely used in Chinese medicine for their action of clearing heat, detoxicating, relieving cough, dispelling sputum and tonifying spleen and stomach. The reason why Glycyrrhiza uralensis has potent and significant actions is that it contains various active secondary metabolites, especially glycyrrhizic acid. In the present study, we cloned the cDNA coding 3-hydroxy-3-methylglutary CoA reductase (HMGR) involved in glycyrrhizic acid biosynthesis in Glycyrrhiza uralensis. The corresponding cDNA was expressed in Escherichia coli as fusion proteins. Recombinant HMGR exhibited catalysis activity in reduction of HMG-CoA to mevalonic acid (MVA) just as HMGR isolated from other species. Because HMGR gene is very important in the biosynthesis of glycyrrhizic acid in Glycyrrhiza uralensis, this work is significant for further studies concerned with strengthening the efficacy of Glycyrrhiza uralensis by means of increasing glycyrrhizic acid content and exploring the biosynthesis of glycyrrhizic acid in vitro.

Objective To describe the prevalence and risk factors of suicidal ideation and suicide attempt among community patients and their family members in Beijing.Methods Accidental sampling method was used in this cross-sectional study to select patients and their family members who visited a community healthcare center in Beijing from June to September 2008.The subjects were required to complete essential information questionnaire,Beck Depression Inventory,Beck Anxiety Inventory and Beck Hopelessness Scale.Chi-square test and binary logistic regression were used to learn the risk factors of suicidal ideation and suicide attempt.Results A total of 2791 valid questionnaires were collected,with a response rate of 90.0％.The participants included 1074 males and 1717 females with mean age (36.8±13.1)years old.The one-year prevalence of suicidal ideation and suicide attempt was 2.8％ (77/2791) or 1.1％ (30/2791),respectively.The one-year prevalence of suicidal ideation was significantly different among the subjects with different professions (x2=12.512,P=0.006),negative events (x2=53.287,P＜0.001),family history of suicide (x2=103.922,P＜0.001),family history of psychiatry diseases (x2=20.640,P＜0.001),hopelessness (x2=77.337,P＜0.001),depression (x2=135.918,P＜0.001) and anxiety (x2=70.303,P＜0.001).The one-year prevalence of suicidal attempt was statistically different among the subjects with different professions (x2=7.954,P=0.037),family history of psychiatry diseases (x2=6.257,P=0.003),hopelessness (x2=29.838,P＜0.001),depression (x2=21.352,P＜0.001) and anxiety (x2=10.014,P＜0.001).Multivariate logistic analysis found that the most important risk factors of suicidal ideation were depression (for mild,moderate and severe depression:odds ratio (OR) 2.38,5.55 and 16.21,respectively; 95％ confidence internal (CI) 1.07-5.31,2.31-13.37 and 6.93-37.92,respectively),family history of suicide (OR=1 1.68,95％CI 5.03-27.10),hopelessness (for mild,moderate and severe hopelessness:OR 3.65,4.25 and 5.02,respectively; 95％ CI 1.55-8.56,1.70-10.65 and 1.46-17.26,respectively),negative life events (OR=2.25,95％ CI 1.35-4.45).The most important risk factors for suicide attempt were hopelessness (for mild,moderate and severe hopelessness:OR 1.09,5.58 and 7.62,respectively; 95％ CI 0.36-3.34,2.03-15.30 and 1.50-38.72,respectively),depression (for mild,moderate and severe depression:OR 1.08,0.27 and 3.02,respectively; 95％ CI 0.43-2.75,0.03-2.29 and 1.05-8.75,respectively) and family history of psychiatry diseases (OR=3.00,95％CI 1.07-8.46).Conclusions Hopelessness and depression could increase the risk of suicide behaviors.Family clinicians should be trained to identify such risk tactors and provide appropriate mental health intervention.

Objective To compare the diagnostic criteria of metabolic syndrome(MS) developed by the International Diabetes Federation(IDF) in 2005,the 3th Report of National Cholesterol Education Program-Adult Treatment Panel Ⅲ(NCEP-ATP Ⅲ) in 2005,and the Chinese Diabetes Society(CDS) in 2004.Methors A total of 1039 adults aged 23 to 62 year-old were enrolled in this epidemiological investigation to assess the prevalence of MS by above three definitions.Results The MS prevalence rate was 14.8%,14.2% and 10.7% in ATPⅢ,IDF,and CDS,respectively.The diagnoses agreement of IDF with ATPⅢ was stronger(Kappa=0.912) than IDF with CDS(Kappa=0.466) and ATPⅢ with CDS (Kappa=0.504).CDS definition found 5.7% of non-MS individuals had risk factor accumulation.Those defined by ATPⅢ and IDF criteria were presented with central obesity + ypertriglyceridemia + abnormal blood pressure and central obesity + hypertriglyceridemia + low hish-density lipoprotein hyperlipidemia.However,those defined by CDS criterion were commonly presented with obesity + hypertriglyceridemia + abnormal blood pressure or obesity + hypertriglyceridemia + hyperglycemia.Conclusions The agreement of IDF and ATPⅢ definition was relatively stronger.For better screening sensitivity,those three criteria,or CDS and IDF criteria,or CDS and ATPⅢ criteria should be used together.

Objective To explore and clarify the clinical features and management of patients with Castleman disease.Methods The clinicopathological features and therapeutic method of 65 patients with Castleman disease admitted to our hospital continuously from 2005 to 2012 were analyzed retrospectively.The mean follow-up time for the entire series was 40 (15-71) months.Results In contrast to Localized Castleman disease(LCD),Multicentric Castleman disease (MCD) patients were relatively older and exhibit various symptoms and abnormal laboratory tests.We found 44 tumor in LCD group.LCD ones,most of which were hyaline vascular (33/42,78.6％),were more likely originated in retroperitoneum,enterocoelia(10/44,22.7％) or mediastinum(10/44,22.7％).The initial treatment for LCD patients was complete surgical resection and all patients are current alive with no evidence of recurrence.MCD ones always accompanied by fever(9/23,39.1％),anemia(9/23,39.1％)and splenomegaly,and most of MCD patients were plasmacytic variants(14/23,60.9％) or mixed cellular variants (5/23,21.7％).We found 66 tumor in MCD group and they were more likely originated in neck (16/66,24.2％) and groin area (12/66,18.2％).Surgery combined with CHOP-like chemotherapy or CHOP-like chemotherapy alone was the mainstream treatment for MCD ones.21 patients achieved complete remission or partial remission,and only 2 patients died of lung infection.Whether patients could tolerate long-term chemotherapy or not was an important prognostic factor.Conclusions LCD patients had no typical clinical symptoms and could be cured by surgery.Most of MCD patients always had a good prognosis after receiving CHOP-like chemotherapy.

Objective To explore the correlation of S100 protein with the prognosis of patients with primary retroperitoneal liposarcoma. Methods Analyzed the clinical data about 108 patients with primary retroperitoneal liposarcoma managed with surgery from January 2009 to June 2014. All patients were followed up. Patients were divided into S100-positive group(58 patients) and S100-negative group (50 patients) according to the immunohistochemical staining results. The overall survival time and all clinical data between two group were compared. Results All patients with primary retroperitoneal liposarcoma received radical surgical resection for the first time. The overall 5-year recurrence rate were 88.9%(96/108), and the median recurrence time was 32.7 months. The 1-year, 2-year, and 5-year recurrence rates of the S100- positive group were 25.9% (15/58), 53.4% (31/58), 96.6% (56/58), respectively, and the median recurrence time were 26.2 mouths. The 1-year, 2-year, and 5-year recurrence rates of the S100-negative group were 10.0%(5/50), 36%(18/50), 80.0%(40/50) and the median recurrence time were 40.0 mouths. Log-rank test showed that S100 protein expression was significantly associated with postoperative recurrence rates (c2=9.931, P=0.002) and survival time (c2=4.571, P = 0.033). The difference between gender, age, removal of the joint organs and tumor size showed no statistical significance on disease special survival (P>0.05). Cox regression analysis showed that S100 protein expression (OR=1.582, 95%CI:1.005-2.491) and histologic subtype (OR=1.531, 95%CI: 1.254-1.870) were independent risk factors of the prognosis of primary retroperitoneal liposarcoma patients. Conclusions S100 protein played a critical role in retroperitoneal liposarcoma carcinogenesis and its expression may be used as a potential survival predictor in patients with primary retroperitoneal liposarcoma.

OBJECTIVE: To prepare gastroretentive slow- released Tinidazole capsules so that the drug can stay at the stomach and act to infective lesions for a long period of time so as to improve the therapeutic efficacy and reduce the adverse reactions.METHODS: The content of Tinidazole was determined by UV - spectrophotometry, the quality standard was established, the stability was studied, the levels of Tinidazole in saliva were detected with HPLC in human body and in vitro dissolubility and human bioavailability were studied .RESULTS: The capsules were stable in quality.The retentive period in artificial gastric juice has reached lOh and in vitro dissolubility(0.5h - 5h) could be described as zero order dissolution .The bioavailability was 163.6% .CONCLUSION: Gastroretentive slow - released Tinidazole capsule was an effective agent for treating HP - related diseases.