Objective To explore the diagnostic value of subacute thyroiditis by fine needle aspiration cytology(FNAC).Method 3665 patients with thyroid disease were detected by FNAC method in 8 years(from Feb 1990 to Dec 1998).Results 353 cases(9 63%) of subacute thyroidits were definitely diagnosed by FNAC with vital staining.Conclusions As compared with other clinical examination including CT,ultrasonic B and radioimmunological assay.FNAC test is all the more simple,safe and valuable diagnostic method for subacute thyroiditis.

Objective To investigate the relationship between oxidative stress and lipid metabolism characteristics in elderly patients under different status of thyroid function. Methods Eighty-six newly-diagnosed elderly patients with thyroid diseases,83 non-elderly patients with thyroid diseases and 20 normal subjects as control were selected.and 47 hyperthyroidism and 39hypothyroidism in elderly,43 hyperthyroidism and 40 hypothyroidism in non-elderly were diagnosed according to diagnostic criteria of the thyroid disease.The levels of fasting plasma malondialdehyde(MDA),superoxide(SOD)and oxidized low density lipoprotein(OX-LDL)were measured by thiobarhiturates,colorimetric method and enzyme linked immunosorbent assay(ELISA).Meanwhile,lipid profile and thyroid function were also measured,SOD/MDA ratio was calculated. Results Lipid profiles in the elderly hyperthyroidism group were significantly higher than those in the non-elderly group(P＜O.05 or P＜0.01)but were significantly lower than those of normal control group(P＜0.05 or P＜0.01).The plasma MDA[(10.23±6.29)μmol/L vs.(3.66±2.53)μmol/L vs.(7.37±4.58)μmol/L],free fatty acids(FFA)[(0.86±0.58)mmol/L us.(0.45±0.12)mmol/L vs.(0.61±O.46)mmol/L]and SOD levels were significantly higher in elderly patients with hyperthyroidism than in normal control and non-elderly hyperthyroidism groups(P＜0.05 or P＜O.01).In the patients with hypothyroidism,the plasma MDA[(9.03±5.98)μmol/L vs.(6.59±3.18)μmol/L vs.(3.66±2.53)μmol/L],OX-LDL[(387.36±71.04)μg/L vs.(355.22±45.01)μg/L vs.(324.53±56.19)μg/L],TC,LDL-C,non-HDL-C,LP(a) and Apo-B100 levels were significantly higher in the elderly group than in the non-elderly and normal control groups(P＜0.05 orP＜0.01).The SOD/MDA ratio was significantly lower in the elderly patient groups with hyperthyroidism or hypothyroidism than in normal control and non-elderly groups(all P＜0.01).In a multiple step-wise regression analysis.FT4 and FFA were independent factors related tO MDA in hyperthyroidism group,and non-HDL-C and LDL-C were independent factors related to MDA inhypothyroidism group. Conclusions Serious lipid metabolic disorder exists in newly-diagnosed and untreated elderly patients with hyperthyroidism and hypothyroidism.It is suggested that oxidative stress is obviously strengthened in elderly patients with thyroid function disorder and that the degree of oxidative stress is correlated with lipid metabolic disorder.

One hundred and seventeen patients with newly diagnosed autoimmune thyroid diseases(AITDs)and 30 normal controls(NCs)were selected.All the patients were divided into 5 groups:hyperthyroidism group(n=33),subclinical hyperthyroidism group(n=21),hypothyroidism group(n=25),subclinical hypothyroidism group(n=23)and euthyroidism group(n=15).Plasma malondialdehyde(MDA),superoxide(SOD)and inflammatory factors levels were measured.Our results showed that plasma MDA level in dysthyroidism groups was significantly higher than that in NCs group(P＜0.05 or P＜0.01).SOD/MDA ratio in AITD groups Was significantly lower than that in NCs group(P＜0.05 or P＜0.01).In a multiple step-wise regression analysis,serum hishly sensitive C-reactive protein(hs-CRP)and tumor necrosis factor-α(TNF-α)were independent factors of MDA.This study might suggest that plasma MDA level in AITD patients receiving no treatment may increase.MDA may be correlated with hs-CRP or TNF-α levels.

Objective To investigate the growth inhibition effect of evodiamine (Evo) on renal carcinoma 786-0 cells and to explore its molecular mechanism. Methods After treated with Evo, methyl thiazolyl tetrazolium ( MTT) assay was used to detect the vitality of 786-0 cells, flow cytometry was employed to examine the cell cycle distribution in 786-0 cells, and immunoblotting was utilized to determine the expression levels of target proteins related to cell cycle progression. Results Evo remarkably inhibited 786-0 cells vitality in dose-dependent manner. Cell cycle analysis indicated that 786-0 cells were arrested in G2/M phase followed by Evo treatment. Furthermore, the results of immunoblotting showed that Evo up-regulated the protein expression levels of P53, P21 and its downstream target gene CyclinB1 in 786-0 cells. Conclusion Evo treatment can induce 786-0 cell cycle G2/M arrest, and its underlying mechanism might be dependent on the P53/P21 signal pathway.

Objective To investigate the effect of ultrasound technology combined with Seldinger technique in the catheteration of peripherally inserted central catheter(PICC).Methods One thousand and eight malignant tumor patients undergoing PICC catheteration were divided into two groups:those with visible or palpable bouncy vein as the control group(n=540)and those with invisible or impalpable elbow vein as the experiment group(n=468).The control group were treated with PICC catheteration into the elbow vein as normal control group and in the experiment group the catheteration was done into the lower part of the upper arm of the basilic vein or brachial vein as the puncture point under the guidance of ultrasound with Seldinger technique.The two groups were compared in terms of success rate of one time puncturing, success rate of one time catheterization and postoperative phlebitis,venous thrombosis and catheter related infection rate.Result There were no significant difference in the success rate for one time puncturing,while the success rate of one time catheterization in the experiment group was significantly higher than that in the control group and the mechanical phlebitis,venous thrombosis,catheter related infection rate in the experiment group were significantly lower than those in the control group(P<0.001).Conclusion Ultrasound technology combined with Seldinger technique for PICC catheterization can obtain high success rate,lower postoperative complications and provide high security.

ving CQI(P＜0.05).Conclusion CQI program effectively reduces CRBSI incidenee in cancer patients with PICC.

Objective To study influences of intervention in pregnant women with positive thyroid autoantibodies on the thyroid function of babies. Methods A total of 55 pregnant women were enrolled with positive thyroid peroxidase antibody (TPOAb) and/or thyroglobulin antibody (TgAb) during prenatal checkup. They were randomly divided into two groups: intervening group( n= 36, newborn group A) was treated with levothyroxine ( L-T4 ), and non-intervening group ( n= 19, newborn group B) was not treated. 30 cases of pregnant women with negative thyroid autoantibodies served as a normal population control group (newborn group N). Serum TSH, TPOAb, TgAb, TT3, TT4, FT3 and FT4 were measured by high-sensitive immunochemiluminescent assay ,and urinary iodine was also examined in the pregnant women. Fetal plasma TSH, TT3, TT4, FT3, and FT4 levels were measured after cutting the umbilical cord from placenta, and repeated measurements were made by 3-4 weeks and 8-10 weeks postpartum. Results At baseline, serum TSH levels of the pregnant women in intervening and nonintervening groups were significantly higher than that in control group ( P＜0.05 ). Non-intervening group had higher TSH and lower TT3, TT4, FT4 compared with the other two groups (P＜0. 05 or P＜0.01 ). The cord blood TSH levels of the neonates in both group B [(7.06 ± 1.31 ) mIU/L] and group A [(6.23 ± 1.26 ) mIU/L] were significantly higher than that of group N [(5.48±1. 17) mIU/L, P＜0.01 and 0. 05]. By 3-4 weeks postpartum,the serum TSH level [(3.21±0.70)mIU/L] in group B was significantly higher than those in group N [(2.72±0.51)mIU/L] and group A [(2.78±0.42) mIU/L, all P＜0.05]. The serum TSH level in group B [(2.99±0.57) mIU/L] was still higher than those in group N [(2.48±0.68) mIU/L] by 8 to 10 weeks postpartum (P＜0.05 ). Multiple stepwise regression analysis revealed that TSH, TPOAb, and urine iodine levels of mothers were independently related to TSH of their infants. Conclusion When differences in thyroid function exist in pregnant women, these differences also reside in their offspring. The thyroid function in neonates correlates with both the thyroid autoantibodies and thyroid function of their mothers.

Bone tissue engineering may provide an alternative to the repairs to skeletal defects resulting from disease, trauma or surgery. Scaffold has played an important role in bone tissue engineering, which functions as the architecture for bone in growth. In this paper, the authors gave a brief introduction about the requirement of bone tissue engineering scaffold, the key of the design of scaffolds and the current research on this subject.
Biodegradation, Environmental ; Bioprosthesis ; Bone Substitutes ; chemistry ; metabolism ; Mechanics ; Surface Properties ; Tissue Engineering ; methods

Objective:To reconstruct the three-dimensional (3D) dose distribution in radiotherapy based on the convolutional neural networks (CNN) through multi-perspective scintillation light processing.Methods:First, fluorescence images were captured from three orthogonal perspectives using a complementary metal-oxide-semiconductor (CMOS) imaging sensor. Then, the images were converted into 3D images, which were input to the trained CNN for dose reconstruction. Finally, the reconstructed doses in different fields were evaluated in terms of gamma pass rate, mean-square error (MSE), percentage depth dose (PDD), and cross beam profile (CBP). Additionally, as the CNN model, 3D-Unet was pre-trained on a virtual dataset.Results:With the 50% maximum dose of as the threshold and 3%/3 mm as the standard, the central-plane and stereo-mean gamma pass rates of all field reconstruction distributions were over 90%, with MSEs remained below 1%. Besides, the PDD and CBP curves showed MSEs below 1‰ and below 1%, respectively.Conclusions:The deep learning-based method for 3D dose reconstruction using scintillation light contributes to enhanced verification of instantaneous 3D relative dose based on plastic scintillation detectors.

By reviewing modern research and integrating clinical practice， this paper elucidates the correlation between the traditional Chinese medicine theory of pathological accumulation from collateral obstruction and gap junction intercellular communication （GJIC）， as well as its theoretical connotation and clinical application in tumor prevention and treatment. Physiologically， gap junction and collateral channels share similarities in structural distribution， substance exchange and information transmission. Pathologically， metabolic coupling mediated by dysfunctional gap junction resembles collaterals stagnation， forming the basis of tumor pathogenesis. The establishment of heterotypic gap junction parallels collateral hyperactivity， contributing to tumor metastasis. The post-translational modifications （PTMs） disorder of connexins is similar to the deficiency of collaterals， serving as a driver of tumor progression. Clinically， tumor treatment should follow the pathomechanism of collateral obstruction leading to pathological accumulation. In the early stage， detoxifying and unblocking collaterals can restore intercellular communication and inhibit tumorigenesis； in the progressive stage， calming hyperactivity and suppressing aberrant collateral pathways can prevent metastasis by interrupting heterotypic gap junction formation； and in the terminal stage， supporting vital qi and modulating PTMs of connexins can help delay tumor progression.