[Objective] To investigate the relationship between Survivin,Caspase-3 and cell apoptosis in osteosarcoma.[Method]The expression of Survivin and Caspase-3 in osteosarcoma were detected by immunohistoehemistry.The apoptosis of osteosarcoma cell was assessed by terminal deoxynucleotide transferase mediated dUTP nick end labeling(TUNEL).[Result]The results showed that Survivin expression was detected 77% in osteosarcoma tissues and was significantly higher than in the normal tissues.Caspase-3 was detected in 26 of 40(65%)osteosarcoma tissues and in 34 of 38(89%)in normal bone tissues,with significant difference(P