Two pairs of primers specific to small subunit ribosomal DNA of Plasmodium falciparum were designed and the expected SSUrDNA fragment was amplified for detecting P.falciparum infection with double-ternperature-nested polymerase chain reaction using DNA prepared by boiling method. The results showed that the nested PCR could amplify a constant size of desired SSUrDNA fragment of P. falciparum which was further confirmed by digestion of restrlction endonuclease and could detect parasitemia level of 0. 8 ? 10-6. It has great potentials for identifying Plasmodium species in ring form of erythrocytic stage and detecting mixed Plasmodium infections. Therefore, it is suggested that this method is sensitive, accurate, simple and rapid in detecting Plasmodium falciparum in blood samples for malaria diagnosis.

ObjectiveTo evaluate the therapeutic effect of Salvia miltiorrhiza and ligustrazine combined with vinpocetine in patients with cerebral infarction.MethodsAtotal of 206 patients with cerebral infarction were randomly divided into a controlgroup and a combined treatment group according to the random number table, 103 in each group. The control group was treated with aspirin and vinpocetine, the combined treatment group received Salvia miltiorrhiza and ligustrazine injection on the basis of the control group. The serum contents of malondialdehyde (MDA) and nitric oxide (NO), as well as the serum levels of superoxide dismutase (SOD) and nitric oxide synthase (NOS) were detected before and after the treatment. The electroencephalography (EEG) amplitudes and the regional cerebral blood flow (rCBF) were recorded before and after the treatment .ResultsIn the combined treatment group, the serum content of MDA was significantly decreased (151.36 ± 11.36 mmol/Lvs.62.23 ± 4.11 mmol/L;t=74.878,P<0.01), while the serum content of NO (110.31 ± 9.51 mmol/Lvs.154.23 ± 12.21 mmol/L;t=28.801,P<0.05) and the serum levels of SOD (55.52 ± 4.47 U/mlvs.85.39 ± 7.21 U/ml;t=35.735,P<0.05) and NOS (115.21 ± 8.39 mmol/Lvs.190.12 ± 11.29 mmol/L;t=54.049,P<0.01) were significantly increased than before the treatment. The rCBF (39.39 ± 2.45 ml/100 g?minvs. 69.95 ± 4.25 ml/100 g?min;t=32.385,P<0.05) and EEG (62.41% ± 4.58%vs.97.02% ± 9.67%;t=31.012,P<0.05) in the combined treatment group were significantly increased than before the treatment. The total efficiency rate in the combined treatment group was significant higher than that in the control group (88.3% vs. 66.9%;χ2=12.343, P=0.004).ConclusionsSalvia miltiorrhiza and ligustrazine combined with vinpocetine has a beneficial therapeutic effect in patients with cerebral infarction, and it is probably associated withincreasing cerebral blood flow and enhancing antioxidant activity.

Objective To improve the specific nursing standards of neostomy and chronic wounds,make patients receive professional guidance and standard treatment. Methods To establish professional team in the neostomy and conduct systematic and specialized training for team members, to work out nursing standards in the neostomy and execute nursing consultation and discussion in team on special types of chronic wounds. Results The professional knowledge of the team members and clinical nurses was enhanced, 18lessons about 50 class-hours were given, the attendance rote reached above 96% since the establishment of professional team in the neostomy, the quality of clinical nursing was improved and the complications were reduced. Conclusions The establishment of professional team provides clinical nurses with a platform of communication and learning, reflect their own value, and promote development of nursing science.

Objective To observe the human papillomavirus(HPV) prevalence and its distribution character among females in Changzhou and to provide epidemiological data for the prevention of cervical cancer and development of HPV vaccine .Methods The clinical material consisted of 32215 women ,who were from Changzhou Hospital T .C .M ,Changzhou Maternal and Child Health Care ,The Second People's Hospital of Changzhou and Changzhou Wujin Hospital from January 2014 to June 2016 .17 kinds of high-risk subtypes (HPV16 ,18 ,31 ,33 ,35 ,39 ,,45 ,51 ,52 ,53 ,56 ,58 ,59 ,66 ,68 ,73 ,82) and 6 kinds of low-risk subtypes (HPV6 ,11 ,42 ,43 ,81 ,83) were detected .All cases were divided into six groups according to the age (<20 ;>21 to 30;31 to 40 ;41 to 50 ;51 to 60 ;and>61) and the age distribution of women infected with HPV and genotypes of HPV were analyzed .Results Out of 32215 cervical cell specimens ,8396 cases were detected HPV infections with the position rate of 26 .06% .In seventeen types of high-risk HPV ,HPV 16 (4 .55% ) were the most common subtype ,followed by 52 (4 .43% ) ,58 (2 .75% ) ,53 (2 .42% ) ,51 (2 .16% ) ,68 (1 .85% ) ,56 (1 .73% ) ,33 (1 .40% ) ,18 (1 .38% ) ,66 (1 .33% ) ,59 (1 .26% ) ,39 (1 .08% ) ,31 (1 .02% ) ,35 (0 .89% ) ,45 (0 .42% ) ,73 (0 .26% ) ,and 82 (0 .15% ) .Six types of low-risk HPV were detected ,of which HPV 81 (2 .96% ) was the most common subtypes ,followed by 43 (1 .86% ) ,42 (1 .62% ) ,6 (1 .27% ) ,11 (0 .80% ) and 83 (0 .26% ) .Conclusion The predominant genotypes of HPV 16 and ,52 infection in female reproductive tract are the highest .

Objective To identity factors influencing willingness to participate in shoulder and neck rehabilitative exercise in laryngeal cancer patients after neck dissection surgery, and to provide reference for subject recruitment in research of similar kind. Methods Between December 2014 to December 2015, when study was conducted to subjects participating in shoulder and neck rehabilitative exercise in laryngeal cancer patients after neck dissection surgery, demographic and disease-related data of patients in two groups (those who were willing /unwilling to participate in the study) were retrospectively analyzed. Binary Logistic regression analysis was used to identify predictive factors influencing willingness to participate in shoulder and neck rehabilitative exercise in laryngeal cancer patients after neck dissection surgery. Results It was revealed by single factor analysis that differences of the following variables between the two groups were statistically significant: age (t=-3.12, P=0.001), whether living in solitude (χ2=4.55, P=0.030), whether currently employed (χ2=9.92, P=0.002), whether with other chronic diseases (χ2=7.91, P=0.004), and whether with radiotherapy after surgery (χ2=4.95, P=0.030); no statistical differences were found regarding tumor location, staging, or neck dissection method (P>0.05). Binary Logistic regression analysis indicated that age (OR=0.71, P=0.001), living alone (OR=0.76, P=0.005) and postoperative radiotherapy (OR=0.88, P=0.001) were predictors of patients' participation in shoulder and neck rehabilitation exercise. Conclusions Certain demographic factors may play a role in the patients' decision on whether or not to participate in shoulder and neck rehabilitation exercise. Senior patients and patients who live alone showa bigger tendency of refusing to attend the exercises. Also, postoperative radiotherapy reduces the patients' willingness in participating in shoulder and neck rehabilitation exercise.

Swallowing function rehabilitation has great significance in improving life quality of patients partial laryngectomy. This paper summarized the main causes, methods of subjective and objective assessment as well as its advantages and disadvantages of patients with dysphagia after partial laryngectomy to set up measurement standard so as to provide a basis for rehabilitation nursing of impaired swallowing.

Objective:To search and summarize the relevant evidences for perioperative hydration in prevention of contrast-induced acute kidney injury (CIAKI) in patients undergoing coronary intervention, and to provide evidence-based basis for clinical nursing staff to develop personalized perioperative hydration plan and prevent CIAKI.Methods:Evidence-based nursing was used to search evidences for perioperative hydration in prevention of CIAKI in patients undergoing coronary intervention in UpToDate, National Guideline Clearing-house (NGC) , European Society of Cardiology (ESC) , JBI Evidence Summary Database, Cochrane Database, PubMed, Wanfang Database, China National Knowledge Infrastructure (CNKI) and China Biomedical Literature Database (CBM) , including evidence summary, guidelines, expert consensus and systematic reviews. The search time was from the establishment of the database to February 2019. Two researchers evaluated the quality of included literatures and extracted evidence from literatures that met the quality standards.Results:A total of 9 articles of evidence were included, including 1 evidence summary, 3 guidelines, 3 expert consensus and 2 systematic evaluations. Finally, a total of 10 pieces of evidence from 5 aspects, including CIAKI risk assessment, hydration fluid type, hydration path and timing, hydration rate and urine volume monitoring, were summarized.Conclusions:This study summarizes the best evidence for perioperative hydration in prevention of CIAKI in patients undergoing coronary intervention and provides an evidence-based basis for clinical medical staff.

Objective:To explore the effect of disease intelligent fusion carrier based on the Smart Assistant for Rheumatism (SSDM) APP and the Fengyun Assistant APP of the National Rheumatology Data Center (CRDC) in patients with rheumatoid arthritis.Methods:From September 2020 to September 2021, a total of 186 rheumatoid arthritis patients admitted to Xinxiang Central Hospital were enrolled using convenient sampling method, and divided into the control group and the observation group with 93 cases each by the random number table method. The control group received CRDC APP management, and the observation group carried out disease intelligent fusion carrier management based on SSDM APP and CRDC APP. The self-management ability score, compliance score, and disease activity degree were compared between the two groups.Results:One month after intervention and three months after intervention, the scores of compliance and self-management ability of the the observation group were higher than those of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The disease intelligent fusion carrier management based on SSDM APP and CRDC APP can help rheumatoid arthritis patients improve self-management awareness and medical compliance behavior.

Objective:To understand the status quo of social alienation in young adults undergoing radioactive iodine (RAI) therapy for papillary thyroid carcinoma (PTC) , analyze its influencing factors, and provide references for the formulation of clinical nursing interventions.Methods:Totally 206 young adults with PTC who underwent RAI therapy at the First Affiliated Hospital of Zhengzhou University from January 2021 to May 2022 were selected by convenience sampling. These patients were surveyed using a general information questionnaire, General Alienation Scale (GAS) , Social Support Rating Scale (SSRS) , and Hospital Anxiety and Depression Scale (HADS) . Multiple linear regression analysis was utilized to analyze the influencing factors of social alienation in young adults undergoing RAI therapy for PTC.Results:The score of social alienation in the young adults undergoing RAI therapy for PTC was (39.39±4.93) . Univariate analysis results indicated that there was a statistically significant difference in the scores of social alienation among the young PTC patients with different marital statuses, levels of disease awareness, employment statuses, family per capita monthly incomes, and tumor stages ( P<0.05) . Multiple linear regression analysis revealed that the influencing factors of social alienation included the level of disease awareness, family per capita monthly income, tumor staging, social support score, and anxiety and depression score ( P<0.05) . Conclusions:The overall level of social alienation in young adults undergoing RAI therapy for PTC is moderate. Healthcare providers should timely assess and adopt targeted intervention measures to enhance patients' sense of social participation.

Objective:To explore the effect of health education based on the Internet in patients with rheumatoid arthritis (RA) .Methods:From January 2019 to January 2020, convenience sampling was used to select 296 patients with RA in Xinxiang Municipal Central Hospital. According to the random number table method, the patients were divided into the control group and the observation group, each with 148 cases. The control group conducted routine health education, and the observation group implemented health education based on the Internet. The treatment compliance, Chronic Disease Self-Management Behavior Scale, the Medical Outcomes Study 36-Item Short Form Health Survey (SF-36) , and Disease Activity Score 28 (DAS28) scores were compared between the two groups after intervention.Results:The treatment compliance of the observation group was 46.62% (69/148) , which was higher than 12.84% (19/148) of the control group, and the difference was statistically significant ( P<0.05) . After the intervention, the self-management score and scores of all dimensions of the observation group were higher than those of the control group, and the SF-36 score was higher than that of the control group, and the DAS 28 score was lower than that of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:Health education based on the Internet can improve the treatment compliance, self-management ability and quality of life of RA patients, and reduce the disease activity.