Objective To discuss the community-based psychological support model for people living with HIV/AIDS. Methods A community-based psychological support team was set up. The team composed of psychology experts,community volunteers,community physicians,community nurses and family members of patients. It provided psychological care and emotional support to people living with HIV/AIDS through activities such as psychological counseling,health education,anti-discrimination advocacy and peer exchanges. Results This model significantly reduced the anxiety and depression,and increased life satisfaction of people living with HIV/AIDS(P<0.01). Conclusions The community-based psychological support model has more advantages than the traditional psychological support. It gives full play to the community on AIDS prevention and control functions,as well as has great significance to mobilize social forces to control the spread of AIDS.

Objective To investigate the incidence of dyssomnia,posttraumatic stress disorder (PTSD) and their relationship in earthquake survivors in Jiangyou and Beichuan area,and supply evidence for prediction and treatment of PTSD.Methods Dyssomnia was accessed by Pittsburgh Sleep Quality Index (PSQI),posttraumatic symptoms were measured using the PTSD Checklist-Civilian Version (PCL-C).Results The prevalence of dyssomnia was 33.8％,the PTSD was 7.5％.In good sleep group,the number of people who didn't suffer from PTSD was 358 (96.5％),the number of people who suffered from PTSD was 13 (3.5％); In dyssomnia group,the number of people who didn't suffer from PTSD was 160(84.7％),the number of people who suffer from PTSD was 29(15.3％).In PTSD negative group,the number of people who had good sleep was 358 (69.1％); the number of people who had dyssomnia was 160 (30.9％).In PTSD positive group,the number of people who had good sleep was 13(31.0％),the number of people who had dyssomnia was 29(69.0％).The correlation analysis showed that total scores of PCL-C were positively related with total score of PSQI.The logistic regression showed that the very hard-hit area,serious posttraumatic symptoms,were the dangerous factors of dyssomnia.Conclusions The dyssomnia and PTSD are still common among victims two years after 5.12 earthquake.PTSD can cause sleeping problem,meanwhile sleep problem could increase the risk of PTSD.

Objective To explore the characteristics of operation,curative effects and operative management in elderly patients of age older than 70 years with coronary heart disease receiving coronary artery bypass grafting.Methods 108 elderly patients of age older than 70 years with coronary heart disease were divided into two groups:OPCAB group(n = 76) and CCABG group (n = 32) The clinical curative effects, early postoperative mortality and complications of the two groups were compared and analyzed respectively. Results OPCAB group was better than CCABG group in these series(P ＜ 0.05): The early postoperative mortality (5.8%, 11.2%)、 myocardial infarction (2.9%, 10.6%), respiration failure(8.7%, 17.5%), pulmonary complications: (11.8%, 31.5%) 、complication of CNS:(1.8% ,9.8%) 、acute renal failure(1.8% ,6.2%) ,the time of intubation: (9.3 ±4.5), (25.4 ±7.5) h,ICU stay(3.1 ± 1.8) ,(7.1 ±2.9) d,hospital stay(15.5 ±8.6) ,(26.4 ±8.6)d. Conclusion OPCAB could reduce operative mortality and complication, it should be the first option for the surgery of elder patients with coronary heart disease;surgical skills and correct perioperative management were the key factors to assure surgical outcome.

Objective To estimate the influences of N-acetyl cysteine (NAC) on a chemical hypoxiamimetic agent CoCl2 induced-injury to, and expressions of inflammatory factors by, an immortal human skin keratinocyte line HaCaT. Methods HaCaT cells were treated with CoCl2 of 2000 μmol/L for 4 hours to set up a chemical hypoxia-induced cell model of skin injury. NAC of various concentrations ( 1000, 2000, 3000 μmol/L)was used to pretreat HaCaT cells for 2 hours prior to the establishment of cell model. After these treatments,cell viability was detected by cell counting kit 8 (CCK-8), the levels of interleukin 6 and 8 (IL-6 and -8) and tumor necrosis factor α (TNF-α) in culture supernatant by ELISA kits, mitochondrial membrane potential (MMP) by rhodamine 123 (Rh123) staining and photofluorography, intracellular reduced glutathione (GSH)content by glutathione detection kit. Results An obvious decline was observed in HaCaT cell viability after pretreatment with various concentrations of NAC for 2 hours. The treatment with CoCl2 of 2000 μmol/L for 4 hours induced an elevation in the supernatant levels of IL-6, IL-8 and TNF-α and a decrease in GSH content and MMP, while the pretreatment with NAC for 2 hours retarded the CoCl2-induced increase in IL-6 and IL-8 levels as well as decrease in GSH content and MMP. Conclusion The reactive oxygen species (ROS) scavenger NAC can protect against CoCl2-induced injury to and inflammatory reaction in HaCaT cells, which may be associated with a decrement in oxidative stress.

Aim To explore the effect of chemical hypoxia-mimetic agent,cobalt chloride(CoCl2)on inflammatory reaction in human keratinocytes(HaCat cells).Methods After HaCat cells were treated with CoCl2 at different concentrations to set up a chemical hypoxia-induced cell model of skin injury,cell viability,intracellular reactive oxygen species(ROS),mitochondrial membrane potential(MMP),the levels of both interleukin 6(IL-6)and interleukin 8(IL-8)as well as the expression of heme oxygenase-1(HO-1)were detected.Results The viability of HaCat cells was reduced by CoCl2 at the concentrations from 500 to 3 000 ?mol?L-1,and the higher CoCl2 doses,the lower cell viability was.CoCl2 induced oxidative stress reaction(increasing ROS production and decreasing MMP).CoCl2 induced inflammatory reaction,enhancing the release of IL-6 and IL-8.CoCl2 at concentrations from 1 000 to 3 000 ?mol?L-1 upregulated HO-1 expression in HaCat cells.Conclusion CoCl2 induces not only oxidative stress,but also inflammatory reaction,increasing the release of both IL-6 and IL-8,as well as HO-1 expression.