1.Wechat public platform-based health information push service
Rui ZHANG ; Jingliang GU ; Zhaoxia SHANG ; Peimin JIA ; Yongxuan DUAN ; Yuan YUE ; Xiaofei SUN
Chinese Journal of Medical Library and Information Science 2015;(5):28-30,34
After health information push service on Internet was investigated ,suggestions were put forward for impro-ving the health information service by making use of the Wechat public platform according to the incomplete and non-professional health information service , rampant advertisements and unaccessible personal information on Internet .
2.Diamide and cyclosporin A enhanced arsenic trioxide-induced apoptosis in NB4 cells.
Yun YU ; Peimin JIA ; Ying HUANG ; Xun CAI ; Guoqiang CHEN
Chinese Journal of Hematology 2002;23(5):254-257
OBJECTIVETo investigate the effects of mitochondrial membrane permeability transition pore (MPT)-opened agent diamide and MPT-closed agent cyclosporin A on arsenic trioxide (As(2)O(3))-induced apoptosis in acute promyelocytic leukemia (APL) cell line NB4.
METHODSNB4 cells were treated with As(2)O(3) alone or in combination with diamide or cyclosporin A in different concentrations. Cell apoptosis was assessed by the morphological observation, Annexin-V assay, distribution of cellular DNA contents and genomic DNA electrophoresis. The mitochondrial transmembrane potentials (DeltaPsim) were detected by flow cytometry according to the intensity of rhodamine 123 uptake in cells.
RESULTSBoth diamide and cyclosporin A significantly enhanced As(2)O(3)-induced apoptosis in NB4 cells. The DeltaPsim collapse induced by As(2)O(3) was also enforced by combined treatment with diamide or cyclospo-rin A. 1 micromol/L As(2)O(3) alone treatment for 72 hours led to DeltaPsim disruption in 27.9% of cells, while combined treatment of As(2)O(3) and diamide or cyclosporin A increased DeltaPsim disruption cells to 59.7% and 42.2%, respectively.
CONCLUSIONSAs(2)O(3)-induced DeltaPsim disruption possibly involves with thiol oxidation or crosslink of important components especially ANT-related molecules.
Antineoplastic Agents ; pharmacology ; therapeutic use ; Apoptosis ; Arsenicals ; pharmacology ; therapeutic use ; Cyclosporine ; pharmacology ; Diamide ; pharmacology ; Drug Synergism ; Enzyme Inhibitors ; pharmacology ; Humans ; Leukemia, Promyelocytic, Acute ; drug therapy ; pathology ; Membrane Potentials ; drug effects ; physiology ; Mitochondria ; physiology ; Oxides ; pharmacology ; therapeutic use ; Sulfhydryl Reagents ; pharmacology ; Tumor Cells, Cultured
3.Advances in the research and applications of orange fluorescent protein.
Wen PENG ; Peimin HE ; Dingji SHI ; Rui JIA
Chinese Journal of Biotechnology 2020;36(6):1060-1068
Fluorescent proteins can be used as probes to investigate intercellular molecular interactions and trace the pathway of specific metabolites, thus providing a detailed and accurate description of various metabolic processes and cellular pathways in living cells. Nowadays, the existing fluorescent proteins cover almost all spectral bands from ultraviolet to far-red. These fluorescent proteins have been applied in many fields of bioscience with the help of high-resolution microscopy, making great contributions to the development of biology. It is generally agreed that orange fluorescent proteins refer to the fluorescent proteins at the spectral range of 540-570 nm. In recent years, researches on orange fluorescent proteins have made great progress, and they have been widely applied in the field of biology and medicine as reporter protein and fluorescence resonance energy transfer as fluorescent receptor. This paper reviews the studies in the field of orange fluorescent proteins over the last 15 years, with the special focus on the development and application of orange fluorescent proteins to provide the basis for the future studies.
Biosensing Techniques
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trends
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Fluorescence Resonance Energy Transfer
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Luminescent Proteins
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metabolism
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Research
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trends
4.Effects of light quality on cell growth and psbA promoter of engineered Synechococcus sp. PCC7002.
Yihua SUN ; Chunli ZHANG ; Dingji SHI ; Xiaohui JIA ; Rui JIA ; Peimin HE
Chinese Journal of Biotechnology 2016;32(9):1286-1290
Light quality can regulate both psbA genes and vector promoter psbA of the engineered Synechococcus. Through light regulation, we tried to improve yield of the recombinant protein for vp28 gene-expressed Synechococcus sp. PCC7002. To drive photon-capturing efficiently, three limiting factors (irradiance, temperature and pH) were optimized by measuring net photosynthesis. High cell density cultures were performed with variant ratios of white, red and blue light in a 5-L photo-bioreactor. Yields of biomass, expressions of vp28 and transcription levels of psbA were compared. High ratio blue light-induced vp28 transcription had tripled and the relative accumulation of VP28 protein was doubled. The relative expressions of psbAII and psbAIII had positive correlations with higher ratio of blue light, not the red light. With high ratio red light inducing, dry biomass reached 1.5 g/L in three days. Therefore, we speculated that red light accelerated biomass accumulation of the transgenic strain and blue light promoted transcription for PpsbA and psbA. These results provided useful information for mass production of cyanobacteria and its secondary metabolites.
Gene Expression Regulation, Bacterial
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Light
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Photosystem II Protein Complex
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genetics
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Promoter Regions, Genetic
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Synechococcus
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genetics
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growth & development
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radiation effects
5.Pathogenicity of white-spot syndrome virus in Macrobrachium nipponensis via different infection routes.
Rong YIN ; Yuanyuan GUO ; Zhangliang WEI ; Dingji SHI ; Peimin HE ; Rui JIA
Chinese Journal of Biotechnology 2017;33(6):946-956
Macrobrachium nipponensis is delicious and has high economic value, but its susceptibility to white-spot syndrome virus (WSSV) is unknown. Susceptibility, morbidity, and multiplication of WSSV in M. nipponense were studied by epidemiological survey, infection experiment and qPCR. M. nipponense was the natural host of WSSV, and the natural carrying rate was about 8.33%. M. nipponense could be infected with WSSV via oral administration, muscle injection and immersion, and the cumulative infection rate of 10 d exposure was 100%, and the cumulative mortality rates were 100%, 75% and 0%, respectively. The infection of WSSV is fast by muscle injection. The virus content after 5 day's injection is 1 000 times higher than that of the first day of infection, and the mortality rate reached 100% after 8 days. The median lethal dose (LD₅₀) measured as the mortality of infected M. nipponense via injection indicated the LD₅₀ in the concentration of WSSV of 2.71×10⁵ virions/μL. In shrimp farming, M. nipponense can be infected by ingesting WSSV infected shrimp or dead shrimp, and also by soaking in WSSV-containing water and thus become a vector, consequently affecting the spread and pathogenicity of WSSV.