1.PCR in Evaluating the Effect of Allicin and Its Combination with SMZco on Murine Toxoplamosis
Lianyu SHAN ; Xiuzhen YANG ; Peimei LIU
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(04):-
Objective To evaluate the effect of allicin alone or combined with SMZco on murine toxoplasmosis by aspecific, rapid, and sensitive PCR technique. Methods 147 mice were infected with 2?10~4 tachyzoites intraperitoneallyand divided into 5 groups at random. Each group was divided into two sub-groups except an untreated group. One sub-group was used to get samples for PCR test and the other for observing the survival duration. The therapeutic grouping wasas follows: group A, a combination of allicin and SMZco administered orally for 7 days and continued by allicin alone till 21days; group B, the combination administered for 14 days and continued with allicin till 21 days; group C, allicin alone for21 days; group D, SMZco alone for 7 days; group E, untreated control. The dosage was: SMZco 400 mg/(kg?d) and al-licin 35 mg/ (kg?d). PCR test was used to detect the parasites in samples of liver and blood from infected mice at 5, 10, 15,20, 25, 30, 40 and 50 days after infection. Results Parasites were eliminated in the blood because no signal was seen inall the blood samples except for samples from group C at day 5 after infection. From day 10 after infection until the end ofthe experiment, no amplification of DNA was seen in all the samples. As for liver samples, signals were clear at day 5 postinfection. From day 10 post infection till the day 50 post-infection, parasites were still detected, but the PCR products de-creased significantly than that of day 5 post-infection. Result showed that a combination of SMZco with allicin provided asignificant protection. SMZco alone was also effective, but allicin alone was not. Conclusion When SMZco is used incombination with allicin, a much higher efficacy is received in the treatment of acute murine toxoplasmosis.
2.Construction and implementation of early clinical practice course in medical colleges and universities
Lijun YUAN ; Yihan WEI ; Xiaoxia LI ; Weiqing WANG ; Peimei LIU
Chinese Journal of Medical Education Research 2015;14(6):601-604
Tianjin Medical University set up the module of Early Clinical Practice in the 2012 year's new curriculum system which takes the teaching mode of combining the in-class curriculum and the extracurricular scientific research as well as the social practice.The in-class curriculum refers to the early contact clinical curriculum,namely making the early contact clinical practice training as a compulsory course,which focuses on the cultivation of students' medical professional ethics and the cultivation of doctor-patient communication ability,health promotion and preventive medicine consciousness,to strengthen students' clinical thinking,clinical research ability and basic skills training.The extracurricular part mainly takes the innovation credit project as the instruction,which will make the social practice,scientific and technological innovation activity project,clinical basic skills training comprehensive,and train students' ability of organization plan,communication and coordination,word expression and scientific research ability.
3.Drug susceptibility testing and randomly amplified polymorphic DNA analysis of Staphylococcus aureus from 178 children with impetigo
Caiyun BIAN ; Yonghong LU ; Peimei ZHOU ; Min WANG ; Ming CHEN ; Cunhuo JIANG ; Qi CAI ; Xuan LI ; Dawei ZHANG ; Qin RAN ; Haitao XU ; Qianming CHEN ; Yan LIU ; Bo WU ; Zhenyuan WANG ; Nayi CHEN ; Danli XIANG ; Xulei HUANG ; Yong BAO ; Yan LV ; Yafei YIN
Chinese Journal of Dermatology 2012;(11):767-770
Objective To test the antimicrobial susceptibility of Staphylococcus aureus from children with impetigo,and to assess the differences in randomly amplified polymorphic DNA profiles between sensitive and resistant Staphylococcus aureus strains.Methods Secretion specimens were obtained from the impetiginous lesions of 178 children,and subjected to bacterial culture.The susceptibility of 162 Staphylococcus aureus isolates against 21 antibiotics was tested.Randomly amplified polymorphic DNA PCR(RAPD-PCR)was performed to characterize the genotype of Staphylococcus aureus.Results Totally,180 bacterial strains were isolated from 178 children with impetigo in Chengdu,including 162(90.00%)Staphylococcus aureus strains.Of the 162 Staphylococcus aureus strains,148 were methicillin sensitive Staphylococcus aureus(MSSA),14 methicillin resistant Staphylococcus aureus(MRSA).The most active antibiotic was minocycline,followed by teicoplanin,quinupristin,vancomycin and nitrofurantoin,while the resistance rate to penicillin was highest,followed by that to erythromycin,clindamycin,compound sulfamethoxazole and tetracycline.All the Staphylococcus aureus isolates were sensitive to fusidic acid,nitrofurantoin,vancomycin,minocycline and teicoplanin.According to RAPD-PCR,the 162 Staphylococcus aureus strains were divided into 8 genotypes,with the three most prevalent genotypes being Ⅲ(31.48%),Ⅱ(26.54%)and Ⅵ(25.93%),which accounted for 65.43%(106/162)in all the strains.The 148 MSSA strains fell into 8 genotypes,with genotype Ⅲ(50 strains,33.78%),Ⅵ(39 strains,26.35%)and Ⅱ(33 strains,22.30%)being the most prevalent genotypes;the 14 MRSA strains fell into 3 genotypes,i.e.,genotype Ⅱ(10 strains,71.43%),Ⅵ(3 strains,21.43%),and Ⅲ(1 strain,7.14%).Conclusions Staphylococcus aureus is the most prevalent pathogenic bacteria in children with impetigo in Chengdu area,which is highly sensitive to minocycline,teicoplanin and quinupristin,and falls into 8 genotypes according to RAPD-PCR with genotype Ⅲ being the most common genotype.