BACKGROUND:In the treatment of teeth later fixed appliance, whether the expansion effect can be maintained? Whether the expansion in the process of mandibular rotation could be improved? Whether the high angle cases are suitable for rapid palatal expansion? OBJECTIVE:To sum the effect of rapid maxil ary expansion combined with fixed orthodontic technique on the occlusion craniofacial three-dimensional direction, hard and soft tissues and temporomandibular joint, as wel as its long-term stability. METHODS:The CNKI database, PubMed database and Wanfang database were searched for the academic journals and literatures on rapid maxil ary expansion combined with fixed appliance with the key words of“maxil ary expansion, fixed appliance, high angle, stable”. The analysis function of database was used. RESULTS AND CONCLUSION:Rapid maxil ary expansion can effectively obtain arch expansion, increase the arch width, and provide space of align teeth required. Fixed appliance can effectively control the tooth movement in three-dimensional space, precisely adjust the teeth, and make the tooth rows into the ideal arch form. Combination of these two kinds of appliances can obtain the complementary efficiency advantages of the appliances, and shorten the treatment course. Expansion can cause mandibular clockwise rotation and mandibular plane angle increasing, while with the application of upper and lower fixed appliance, the occluding relation between maxil a and mandible can be adjusted more precisely in order to make the mandibular first molar move to the maxil ary molar buccal, establish good occlusal relationship, so that decrease the mandibular plane angle. So the rapid maxil ary expansion combined with fixed orthodontic can be used appropriately in the treatment of the patients with high angle intraversion.

Objective To study the appearances and early fetures of sacroiliac joints(SIJ) in ankylosing spondylitis on X-ray film and CT,and to valuate the CT examination of sacroiliac joints in the same groups.Methods The plain X-ray film of 73 cases of SIJ of ankylosing spondylitis which had been proved clinicalhy were studied.All the patients had been examed with CT at the same time.The appearances presented by the two methods were concluded compared and graded,the relationship between them was analyzed with statistics.Results The erosion,cystic lesions of bone and swollen of soft tissues were the early features of sacroiliac joints in ankylosing spondylitis,but the detection rate of them by X-ray was 73%,38%,0.6%,by CT was 86%,72%,3%.They can find on CT and conventional radiography,but the detection rate of them by CT was much higher than that by X-ray plain film(?

Objective:This study aims to investigate the expression pattern of miR-512-3p in breast cancer and noncancerous paired specimens as well as the effects of miR-512-3p on the proliferation, apoptosis, cell cycle, and cloning of MD-MBA-231 breast cancer cells. The study also aims to identify the miR-512-3p target gene. Methods:Reverse transcription-polymerase chain reaction (RT-PCR) was conducted to quantify the miR-512-3p expression in breast cancer and noncancerous paired specimens. Methylthiazol tetrazolium (MTT) assay, flow cytometry, and clone formation assay were used to characterize the function of miR-512-3p in breast cancer. Target prediction was performed using the TargetScan, PicTar, and miRanda software. The results were validated using RT-PCR and western blot target validation. Results:The relative expression of miR-512-3p in breast cancer specimens was significantly lower than those in normal breast specimens (P<0.05). MTT assay revealed that 48 h after transfection, miR-512-3p significantly repressed the proliferation of MD-MBA-231 cells at a suppression rate of 45.38%and at a concentration of 100 nmol/L. MiR-512-3p increased the percentage of early apoptotic cells in the treatment groups (9.32 ± 0.41)%compared with those in the blank controls (3.1 ± 0.54)%and in the negative controls (2.9 ± 0.39)%(P<0.05). Significant differences were found in the percentages of the G0/G1-and G2/M-phase cells after miR-512-3p transfection compared with those in the controls (P<0.05). In the cloning assay, clone formation was inhibited in the miR-512-3p-transfected groups compared with those in the control groups. RT-PCR and western blot results indicate that miR-512-3p significantly inhibited the c-FLIP mRNA and protein expression. Conclusion:MiR-512-3p expression is relatively decreased in breast cancer specimens compared with those in the normal samples. The negative effect of miR-512-3p on cell proliferation and clone formation and its positive effect on early apoptosis through c-FLIP targeting suggest that miR-512-3p acts as a tumor suppressor gene in breast cancer. Therefore, miR-512-3p may be a new target for the diagnosis and treatment of breast cancer in the future.

Objective To investigate a possible role of orexin in the regulation of estrous cycle by examining the expression of orexin and orexin receptors(OX1R,OX2R) in the rat hypothalamus during the estrous cycle. Methods The levels of prepro-orexin(prepro-OX),orexin-A,OX1R,and OX2R in the rat hypothalamus during the estrous cycle were evaluated by competitive reverse transcription-polymerase china reaction(RT-PCR) assay. Results Only expression OX1R mRNA during late proestrus was significantly higher than that at metestrus.No significant changes of prepro-OX and OX2R mRNA expression were observed during the various estrous cycle phases.Conclusion Orexin might regulate the secretion of GnRH and/or LH by binding OX1R,contributing to the occurrence of ovulation.

Cloud computing is the third technological revolution of IT industry following personal computers and the Internet,which is bound to have a profound impact on hospital informationization.Based on an analysis of the barriers for building the information system in the hospital,we proposed the development and implementation plan of the private cloud.This plan proves the cloud can make server resources more efficient and downsize operating costs significantly.In the end,we discussed the value and perspective of hospital private cloud.

Objective: To realize the requirement of adjacent area accuracy,axial surface physical contour and smoothness in the process of inlay computer-aided design(CAD).Methods:A compromised method was brought forward that some assistant 3-D constraint lines from occlusion to gingiva were used to lead the inlay contour on axial corner and other characterized areas,and two perpendicular 3-D constructing lines at adjacent area were used to construct accurate 3-D configuration of this area.The axial surface was completely defined by the network of specified curves which were two constructing lines and four borders.Results:Inlay axial surface was smooth,the contour was consistent with the residual tooth body and the 3-D configuration in adjacent area was accurate.Conclusion:It is feasible to realize the inlay axial surface accuracy,smoothness,and consistency using the method of special distributed 3-D B-Spline curve network.

Objective To study the changes of imaging features of rats with implanted liver cancer before and after electrochemotherapy (EChT).Methods Immediate cancerous ascites injection method was used to form rats liver cancer model. The imaging features of rats with implanted liver cancer, including tumor size, density, border, enhancement and singal were observed with CT and MRI respectively. Results The characteristic magings showed all these rats bearing single, round tumor in liver seven days later. Low density lesions were seen in pre contrast scans and tumor border intensification were seen on contrast scans. Low signal lesions were found in MRI T 1WI and high signal lesions were revealed on MRI T 2WI. Seven days after EchT, low density lesions were seen in pre contrast scan and non enhanced appearences were seen in contrast scan by CT. High signal and mixed signal lesion were seen in MRI T 1WI and relative low signal lesion were seen in MRI T 2WI.Conclusions Imaging features analysis is useful to assess the therapeutic effect on rats with implanted liver cancer before and after EChT.

Objective To compare the effects of electrochemotherapy (EChT) on implanted liver tumor cells apoptosis of rats.Methods Immediate cancerous ascitis injection method was used for the formation of rats liver tumor model and then followed by rats liver tumor treatment with EChT. Tumor size, necrosis and apoptosis indexes were observed after one week by imaging or pathologic method. The results were compared with those of control group. Results Before EChT, rats liver tumor volume appeared as (100?6)mm 3 and turned to be (125?10)mm 3 one week after EChT, which were significantly smaller than those of the control group [(190?11)mm 3], P

Objective To compare the therapeutic effects of electrochemotherapy (EChT) and radiofrequence ablation (RFA) on rats with implanted liver cancer.Methods Immediate cancerous ascites injection method was utilized for creation of rats liver cancer model and followed by treatment with EchT and RFA respectively. Tumor size, necrosis and apoptosis were observed one week later by imaging and pathology. The results were compared with those of control group. The survival times were observed also. Results Before treatment, all rats average liver tumor volume was (100?6)mm 3. One week after treatment, average tumor volumes in EChT group [(125?10)mm 3] and RFA group [(143?12)mm 3] were significantly smaller than those of control group [(190?11)mm 3], but tumor volume showed significant statistical difference between EChT group and RFA group. Necrosis, apoptosis and survival times in EChT group and RFA group were significantly higher than those of control group.Conclusions EChT or RFA could promote tumor necrosis, induce apoptosis, inhibit tumor growth and prolong the survival time of rats with implanted liver cancer.

Objective:To explore the route of automatic computer-aided design for occlusal rest of the removable partial denture.Methods: A new module for the design of occlusal rest was developed.The three-dimensional data of a partially dentate dental cast was obtained and imported into the new module.The contour of the occlusal's tissue surface was identified.Then,the tissue surface and polished surface were constructed respectively and connected to each other to form an integrated entity.Results: The surface of the occlusal rest was smooth and the curvature matched that of the surrounding area of the abutment tooth.The accuracy was satisfying.Conclusion: Design of occlusal rest was much more convenient and time-saving using the newly developed module.The future development of special software package for the design of removable partial denture will benefit from it.