Anordrin, which has been widely used as an oral contraceptive tablet in China, administered intra-vaginally at different levels of dose possessed marked antifertiiity effects in Sprague Dawley rats. The anti-implantation efficiency of the compound given intra-vaginally was superior to that of oral dosing in some respects. This kind of medication was feasible, convenient to use and with no serious side effects. Our investigation provides the experimental date for clinical study of using this drug vaginally.