Abdominal incisional hernia is a common complication of abdominal surgery,of which the two major direct causes are surgery wound healing and intra-abdominal pressure.Therefore,the factors causing poor wound healing and intra-abdominal pressure are incisional hernia incentives.Surgery is the only radical method to cure it,including open surgery and laparoscopic surgery.This review contains two contents,the summary of the current research and the prospects.

Caspase proteins play a key role in the process of promoting apoptosis,we can often detect caspase proteins in hepatic ischemia-reperfusion injury and in experimental study on apoptosis.Now studies on caspase proteins in hepatic ischemia-reperfusion injury of experimental field are reviewed.

Children's portal hypertension is a hemodynamic abnormalities syndrome,it refers to children with portal vein pressure exceeds 5 mmHg,or portal vein,hepatic venous pressure gradient exceeds 10 mmHg.Etiology of portal hypertension of children:portal vein obstruction,congenital hepatic fibrosis,biliary atresia.Current treatment methods are:drug treatment,varicose vein ligation or sclerosis,surgical treatment.Although the incidence of children's portal hypertension is relatively low,it can cause various serious complications,such as ascites,gastric variceal bleeding,hepatic encephalopathy and so on.To increase awareness,improve the cure rate and reduce complications,the artical will review the children's portal hypertension etiology,pathogenesis,diagnosis,and treatment progress.

Postsurgical gastroparesis syndrome (PGS) is a functional disease,characterized by nauseating,vomiting,and gastric atony without of mechanical gastric outlet obstruction,and is ofen caused by operation at the abdomen,especially gastric and pancreatic operation.In recent years,the incidence rate of PGS presents ascendant tendency,and more and more doctor attach importance to PGS.A better knowledge of the etiology,pathogenesis,clinical manifestation,diagnosis and treatment of PGS can help decrease the incidence rate of PGS and improve the cure rate of PGS.Thus,the authors write this review about these aspects of PGS.

Gallstone ileus is a rare complication of cholelithiasis,and is common in older gallstones patients.It lacks the typical clinical presentation,so preoperative diagnosis is very difficult.The best diagnostic modality now is abdominal CT scan.Surgery is the most important and useful treatment.According to patient' s own situation,choose individualized surgical approach.Endoscopic,laparoscopic and other minimally invasive treatment are promising.With the development of related technologies,it maybe increased of the gollstone ileus treatment.

OBJECTIVE:To improve HPLC for content determination of ketoprofen in Ketoprofen enteric-coated capsules. METHODS:HPLC method was adopted. The determination was performed on Chiralpak IC column with mobile phase consisted of n-hexane (0.1% TFA)-isopropanol(90:10,V/V)at a flow rate of 0.8 mL/min. The detection wavelength was set at 268 nm,and column temperature was 25 ℃. The sample size was 10 μL. RESULTS:The linear range of ketoprofen were 0.025-0.5 mg/mL(r=0.9998). The limit of quantitation was 1.0 mg/L,and limit of detection was 0.2 mg/L. RSDs of precision,stability and reproduc-ibility tests were lower than 2%;recoveries were 96.36%-100.32%(RSD=1.87%,n=6). CONCLUSIONS:The method is sim-ple,accurate and rapid,and can be used for the content determination of ketoprofen in Ketoprofen enteric-coated capsules.

Objective To observe the effect of Dangui Huoxue Zhitong Granule(Granule of Radix Salviae Miltiorrhizae and Ramulus Cinnamomi for activating blood circulation to stop pain)on pain,inflammation and injury of soft tissues.Methods Animal models of inflammation,pain and traumatic injury of soft tissues were adopted for observing the twisting reaction after intraperitoneal injection of acetic acid,pain threshold of hot-plate test,auricular selling of mice and metatarsal swelling of rats.Results Dangui Huoxue Zhitong Granule could decrease conspicuously the twisting reactions,increase the pain threshold(P

OBJECTIVE:To optimize the basic formula of Compound Salicylic Acid and Chloramphenicol Gel with orthogonal design test.METHODS:The closage of CMC-Na、glycerin and ethanol were taken as the review object with the oligation grade as the evaluation index to conduct L9(33) orthogonal test and the contents of salicyclic acid and the chloramphenicol were determined by HPLC. RESULTS: The optimized basic formula was as follows: sodium carboxymethylcellulose 3 g, glycerine 10 g, and alcohol 35 mL.The average recovery of Salicylic Acid and Chloramphenicol were 99.1% and 101.6%,the RSD were 0.8% and 1.3%.CONCLUSION: The basic formula optimized with orthogonal test is proved to be stable and serve as theoretic basis for the industrialized production of this preparation.

Objective To study the expression of vascular endothelial growth factor(VEGF) in breast cancer and its clinical significance.Methods The expression of the protein was determined by immunohistochemistry in 60 cases of breast cancer and 20 cases of benign breast diseases.The relationship of VEGF expression with clinicopathological characteristics was statistically analyzed.Results Of all the 60 cases of breast cancers,49 cases showed positive VEGF expression,while only 4 cases expressed VEGF in the group of breast adenofibroma.There was significant difference between breast cancer and breast adenofibroma( P ＜ 0.05 ).The overexpression of VEGF was related with the tumor size ( P ＜ 0.05 ),histological grade ( P ＜ 0.05 ),lymph node state ( P ＜ 0.05 ) and TNM stage ( P ＜ 0.05 ),but was irrelevant to age,ER and PR( P ＞ 0.05).Conclusion VEGF might have important significance in the development,metastasis and prognosis of human breast cancer.It may be taken as an independent marker to predict the prognosis of breast cancer.

Objective To establish a human HepG2 cell growth model under the low oxygen environment induced by cobalt chloride in order to observe the impacts of human HepG2 cell proliferation,cellular cycles and apoptosis,namely cellular growth conditions,under the low oxygen environment induced by cobalt chloride with different concentrations and to study the HepG2 cell growth mechanism under the low oxygen environment induced by cobalt chloride.Methods The human HepG2 cells in the logarithmic phase were randomized grouping as control group and CoCl2 experimental group with different concentrations (50 μm/L,100 μm/L,150 μm/L and 200 μm/L).① HepG2 cell proliferation was tested by MTT assay to calculate cell's suppression rate and draw HepG2 cell growth inhibition curves.② The move ability of HepG2 cells was observed by scratch test.③ The cellular apoptosis and periodic changes were detected using the flow cytometer Annexin-V FITC/PI double-staining and PI single staining methods.④HepG2 cell's Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Bcl-2 protein expression were detected by Western Blot.Results ① The test results obtained via MTT assay showed that CoCl2 suppressed the human HepG2 cell proliferation within a certain amount of time and concentration and presented a time-dose dependent relation.② Scratch damage trial suggested that the cobalt chloride suppressed the HepG2 cell migration and wound repair capacity and presented a concentration dependent relation.③ Flow cytometer' s test results revealed that the apoptosis rates (％) in control group and experimental group with different concentrations (50 μm/L,100 μm/L,150 μm/L and 200 μm/L) were 3.42,7.74,13.07,20.56,28.53 and 44.45 (P ＜0.05),respectively.The apoptosis rate of the experimental group was significantly increased compared with the control group,as well as showing a concentration dependency.The results of cellular cycles revealed that the cobalt chloride significantly suppressed the HepG2 cell's periodic changes along with increases of concentration,as well as blocked the cell cycle staying in phase G1,thereby suppressing cell proliferation.④Western Blot test:Compared with the control group,the Bcl-2 protein expression was significantly decreased in the experimental group after treatment of cobalt chloride with different concentrations.Conclusion Within a certain range,CoC12-indiuced low oxygen environment can suppress the human HepG2 cell proliferation and healing migration capacity,induce apoptosis and present a time-dose dependent relation.The mechanism is likely associated with decreases of Bcl-2 protein expression.