1.Clinical Observation on Therapy of Dredging Meridians for Prostatitis Type Ⅲ
Peihua LIANG ; Jianfeng GUI ; Bing DENG
Journal of Guangzhou University of Traditional Chinese Medicine 2004;0(05):-
[Objective] To investigate the effect of dredging meridians in improving prostatitis type Ⅲ. [Methods] Sixty cases of prostatitis were randomized into groups A and B, 30 in each group. The two groups were both treated with Keduohua Capsule, which was a ?-eceptor blocker, and group B was added with Guizhi Fuling Pill (GFP). The treatment lasted 12 weeks. Chronic prostatitis symptoms index issued by American National Institute of Health (NIH-CPSI) , white blood cells (WBC) count and the amount of lecithin body in prostatic secretion, and urinary flow rate were examined before and after treatment to evaluate the therapeutic effect of GFP and the safety of the combination of two medicines . [Results] The clinical symptoms were relieved, WBC count and the amount of lecithin body in prostatic secretion were reduced, and urinary flow rate was increased ( P
2.Determination of Residual Solvent DMF in Imiquimod by HS-GC
Guanghu RUI ; Peihua MIAO ; Yuantai LIANG ; Xuejie SONG ; Feng ZHAO ; Haiqiang ZHOU
China Pharmacist 2014;(9):1586-1588
Objective: To establish a new method for determining residual organic solvent DMF in imiquimod. Methods: The samples were injected into an Agilent HP-PLOT/Q capillary column(30 m × 0. 530 mm,40. 0 μm) by a headspace sampler and ana-lyzed with an FID detector, the carrier gas was nitrogen, the inlet temperature was 250℃, and the detector temperature was 270℃. The column temperature was programmed raised. Results: The resolution among the peaks of DMF and the other residual solvents could meet the requirements. There was a good linearity within the experimental concentration range. The average recovery was 94. 6%(RSD=4. 0%, n=9). The limit of quantification and the limit of detection was 4. 809μg·ml-1 and 0. 963μg·ml-1, respectively. Conclusion:The method is convenient, accurate and sensitive, which can be used in the determination of residual solvent DMF in imi-quimod.
3.Practice and consideration of innovation and entrepreneurship education in higher medical univer-sities
Yongling LONG ; Peihua LIANG ; Yafei SHI ; Qing YE ; Huaxin PAN ; Nenggui XU
Chinese Journal of Medical Education Research 2016;15(12):1215-1219
In the context of Popular Entrepreneurship and Innovation, scientific and systemic implementation of innovation and entrepreneurship education is the focus of higher education reform at present. This paper analyzes the important significances of carrying out innovation and entrepreneurship education in higher education of traditional Chinese Medicine, introduces the specific practices and experi-ences of designing educational concept and constructing education system about innovation and entrepre-neurship education, following the objective laws of modern educational development and the growth pattern of Chinese medicine talents, in order to provide reference for further promoting innovation and entrepreneu-rship education reform.
4.Hot spots analysis for MOOC research of higher medical education in our country based on co-word clustering
Shenqing LI ; Sida CHEN ; Lihuan QIAN ; Jing LI ; Peihua LIANG ; Chunping FANG ; Buping LIU
Chinese Journal of Medical Education Research 2016;15(1):62-66,67
Objective To analyze the hot research spots of MOOC research of higher medical edu-cation in China. Methods We searched the database of China National Knowledge Infrastrucure (CNKI), VIP database of Chinese journal (VIP), Wanfang Database for published studies on MOOC research of higher medical education from 2005 to April 2015. The high frequency words were counted by an analysis program-Bibliographic Item Co-occurrence Matrix Builder (BICOMB). According to the ranking word frequency threshold formula by Donohue, we collected the high-frequency keywords. The corresponding co-word ma-trixes were constructed by Bibexcel, then the data was input into SPSS 19.0 software for hierarchical clus-tering analysis. Results Finally 2 459 articles were included. 11 177 keywords and 13 high frequency words (the number of the real selected was 24) were taken into account. The keywords “stanford” clus-tered with “education module”, “online education”, “online courses”, “distance education”, “learning pro-cess”, “self-regulated learning”, “information technology”, “classroom teaching”. The keywords “network course” clustered with “teaching design”. The keywords “online learning” clustered with itself. The key-words “challenge” clustered with “colleges and universities”. The keywords “flipped classroom” clustered with“micro-lecture”,“MOOC”,“higher education”, “library”, “teaching module”, “teaching reform”, “big data”,“open education”. The keywords“SPOC”clustered with itself. Conclusion The research of MOOC research of higher medical education in our country has formed the basic characteristics with six hot spots involving teaching design, the model of online learning, dispassion thinking, teaching reform, and develop-ment. And there are many potential areas.
5.Practice and thinking of Otorhinolaryngology Clinical Skill Competition
Bing LIANG ; Yuqin FAN ; Peihua WANG ; Yan LIU ; Hao WU
Chinese Journal of Medical Education Research 2021;20(10):1157-1160
This paper introduces the clinical skill competition organized by the faculty of Otolaryngology Department of Shanghai Jiao Tong University School of Medicine, and analyzes the main problems in implementing items of basic life support, single ear compression bandage, debridement and suturing, foreign body removal from pharynx and larynx area, nasal packing and hemostasis and comprehensive skills. According to contestants' performances, their abilities in clinical analysis and operating skills have been tested, and their professional humanities have been cultivated. Thus, the aim of "promoting learning through examination and substituting competition for practice" is achieved through the competition. Clinical skill competitions can be used widely as supplement form of medical education for its practicability, entertainment, innovation and interaction.
6.Development and validation of a nomogram for predicting survival outcomes in prostate cancer patients based on SEER database
Jinxia LI ; Jiapeng HUANG ; Peihua LIANG
Journal of Modern Urology 2023;28(8):696-701
【Objective】 To establish and verify a nomogram model of overall survival (OS) of prostate cancer patients based on the SEER data. 【Methods】 A total of 12 642 patients diagnosed with prostate cancer during 2010 and 2015 were extracted from the SEER database. Patients were randomly divided into the model group (n=8 850) and validation group (n=3 792). The independent risk factors for OS were analyzed with univariate Cox proportional risk regression, lasso regression and multivariate Cox proportional risk regression. A nomogram was constructed to predict the 1-year, 3-year and 5-year OS. The prediction potential of the model was evaluated with the consistency index (C-index), calibration curve and receiver operating characteristic (ROC) curve. 【Results】 Multivariate Cox regression analysis showed that age, T stage, N stage, M stage, bone metastasis, liver metastasis and regional lymphadenectomy were independent risk factors for OS (P<0.05). The seven factors were used to construct an OS nomogram model. The C-index of the modeling set was 0.750, and the area under the ROC curve (AUC) at 1, 3 and 5 years were 0.77, 0.77 and 0.76, respectively;the C-index of the validation set was 0.765, and the AUC at 1, 3 and 5 years were 0.83, 0.79 and 0.76, respectively. The calibration curves of the modelling set and validation set showed a good agreement with the actual survival prediction rate. Risk stratification of patients based on the nomogram model showed that the OS of patients in the high-risk group was significantly lower than that in the low-risk group (P<0.001). 【Conclusion】 The nomogram can be used to predict the prognosis of prostate cancer patients, and is important for individualized treatment plans.
7.Application of multi-touch virtual anatomy system in the otolaryngology teaching
Bing LIANG ; Yuqin FAN ; Hao WU ; Jianqing CHEN ; Peihua WANG
Chinese Journal of Medical Education Research 2020;19(2):238-240
The residents and low-grade resident trainees of the Department of Otorhinolaryngology Head and Neck Surgery of the Ninth People's Hospital of Shanghai Jiao Tong University School of Medicine were selected as subjects. In the multi-touch virtual anatomy system, the lecturers made the anatomical signs of otolaryngology head and neck surgery, and applied them through the training method. Then the teachers explained the three sub-specialties of the nose, throat, neck and ear in the multi-touch virtual dissection table. The teaching method can stimulate the learning interest because of its vivid three-dimensional sense. The characteristics of teaching and learning are beneficial to the cultivation of medical education teachers. In addition, the one-time investment of equipment breaks through the supply bottleneck of traditional anatomical consumables and promotes active learning among medical students. The multi-touch virtual anatomy system can effectively improve the teaching methods, facilitate the interesting teaching, and has broad application prospects in medical teaching.
8. Preliminary study on the application of real-time anatomical demonstration in the theoretical teaching of otorhinolaryngology-head and neck surgery
Bin YI ; Bing LIANG ; Zhaoyan WANG ; Peihua WANG ; Hao WU
Chinese Journal of Medical Education Research 2019;18(10):1018-1021
Objective:
To investigate the effects of real-time anatomical demonstration and traditional teaching in otorhinolaryngology-head and neck surgery.
Methods:
Students who studied this course from Shanghai Jiao Tong University School of Medicine were divided into two groups: clinical students in 5-year program (
9.PXR activation impairs hepatic glucose metabolism partly via inhibiting the HNF4α-GLUT2 pathway.
Peihua LIU ; Ling JIANG ; Weimin KONG ; Qiushi XIE ; Ping LI ; Xiaonan LIU ; Jiayi ZHANG ; Ming LIU ; Zhongjian WANG ; Liang ZHU ; Hanyu YANG ; Ying ZHOU ; Jianjun ZOU ; Xiaodong LIU ; Li LIU
Acta Pharmaceutica Sinica B 2022;12(5):2391-2405
Drug-induced hyperglycemia/diabetes is a global issue. Some drugs induce hyperglycemia by activating the pregnane X receptor (PXR), but the mechanism is unclear. Here, we report that PXR activation induces hyperglycemia by impairing hepatic glucose metabolism due to inhibition of the hepatocyte nuclear factor 4-alpha (HNF4α)‒glucose transporter 2 (GLUT2) pathway. The PXR agonists atorvastatin and rifampicin significantly downregulated GLUT2 and HNF4α expression, and impaired glucose uptake and utilization in HepG2 cells. Overexpression of PXR downregulated GLUT2 and HNF4α expression, while silencing PXR upregulated HNF4α and GLUT2 expression. Silencing HNF4α decreased GLUT2 expression, while overexpressing HNF4α increased GLUT2 expression and glucose uptake. Silencing PXR or overexpressing HNF4α reversed the atorvastatin-induced decrease in GLUT2 expression and glucose uptake. In human primary hepatocytes, atorvastatin downregulated GLUT2 and HNF4α mRNA expression, which could be attenuated by silencing PXR. Silencing HNF4α downregulated GLUT2 mRNA expression. These findings were reproduced with mouse primary hepatocytes. Hnf4α plasmid increased Slc2a2 promoter activity. Hnf4α silencing or pregnenolone-16α-carbonitrile (PCN) suppressed the Slc2a2 promoter activity by decreasing HNF4α recruitment to the Slc2a2 promoter. Liver-specific Hnf4α deletion and PCN impaired glucose tolerance and hepatic glucose uptake, and decreased the expression of hepatic HNF4α and GLUT2. In conclusion, PXR activation impaired hepatic glucose metabolism partly by inhibiting the HNF4α‒GLUT2 pathway. These results highlight the molecular mechanisms by which PXR activators induce hyperglycemia/diabetes.