The hydride generation technique was used to determination of germanium in the presence of L-cysteine.The GeH4 was absorbed and coloured by a mixed solution of 2-(5-NO2-2-pyridylazo)-5-diethylaminophenyl(5-NO2-PADAP),NaClO and (CH2)6NH+.The maximum absorption of the colour complex was at 564nm.Its apparent molar absorption coefficient was 1.84×105L·mol-1·cm-1.Beer＇s law was obeyed for 0～500μg/L germanium.The recoveries of spiked samples were in the range of 89%～97%,while the relative standard deviation was less than 6.3%.This method is sensitive,simple and reproducible.The method has been applied to the determination of Ge in the chinese medicine with satisfactory result.

Objective To investgate the value of interventional therapy in inferior vena cava stenosis or obstruction caused by tumor and management after the operation. Methods 53 patients with IVC stenosis caused by tumor had detailed information. After the guide wire passed through the stenosised part and then undergoing balloon expansion, Gianturco Z stents or wallstents were implanted to undergo venography and measuring pressure after that. Results Technical success was attained in 50 patients, 3 cases was failure because the guide wire couldn't pass through the obstructed region.The technical success rate is 94.3%. 2 patients with IVC stenosis over 10 cm length was placed two stents. In other 48 patients,everyone was placed one stent. The pressure of IVC dropped from (28.66?4.08)cm H 2O (1 cm H 2O =0.098 kPa) before operation to (17.65?4.18)cm H 2O after operation. Through t examination, the difference of IVC pressure before and after treatment has statistic significance( t =3.261, P

Objective To study the expression of CD105 in laryngeal carcinoma and to evaluate the clinical significance of microvessel density(MVD).Methods Pathologic paraffin-embedded sections and clinical data from 40 patients with primary squmamous cell carcinoma of larynx were studied through immunohistochemistry.Microvessel density(MVD) highlighted by CD105 and CD34 were counted.Results CD105 expression in tumour tissue was significantly higher than normal health mucosa.The mean CD105-MVD value of T3 and T4 tumours showed a significantly deeper staining than T1 and T2;The mean CD105-MVD value of tumours with metastasis was also markedly higher than tumours with no metastasis;The diffenerce of mean CD105-MVD value between in 16 patients with recurred laryngeal carcinoma and in 20 patients with unrecurred laryngeal carcinoma for two years of follow-up was markedly significant(P

Object To study the change of serum soluble interleukin 2 receptor(SIL-2R) in syphilitic patients and its clinical significance.Methods The serum SIL-2R level in syphilitic patients of different stages and after therapy were detected using the ELISA technique.Results The serum SIL-2R level in syphiliti patients was higher than that in control group(P0 05).Conclusions SIL-2R level and its variation have some reference value for evaluating the therapeutic effect and judgement of the disease course in syphilitic patients.

Objective To explore the clinical value of CT-guided 125I radioactive seeds implantation and chemical ablation for malignant retroperitoneal tumors.Methods Because of the rejection of the second surgery resection and insensitivity of chemotherapy and radiotherapy,nineteen patients with recurrent or metastasis malignant retroperitoneal tumors were treated by CT-guided125I radioactive seeds implantation according to TPS or Halarism's experienced function,and percutaneous ethanol injection was performed if the way of punctuation Was limited.The extent of pain relief was assessed one month later after therapy.All the patients received enhanced CT scan 6 months after the first treatment,and imaging evaluation wag performed according to WHO criteria.Results For the 19 patients.pain relief Was achieved more or less in all patients.Imaging evaluation revealed complete relief,partial relief,no change in 10,7,2 cases respectively.All patients are still alive now.The longest followed span is 31 months.and the shortest is 7 months,the average followed span is 13.5 months.Conclusion CT-guided 125I radioactive seeds implantation and chemical ablation ore effective for malignant retropefitoneal tumors.

Objective To study the CT findings of pulmonary mucosa-associated lymphoid tissue (MALT) lymphoma. Methods The CT examinations of 12 patients with pathologically proven pulmonary MALT lymphoma were reviewed retrospectively. Evaluated imaging findings included number, distribution,shape, attenuation and other associated findings of each lesion were evaluated. Results Thirty-two pulmonary lesions, including consolidations, masses, nodules and lesions with ground glass attenuation,were identified in 12 patients. Multiple lesions were founded in 10 of 12 patients and solitary lesion in 2 patients. Multiple lesions found in one lung in 2 patients, and multiple lesions found in both lungs in 8 patients. Ten cases demonstrated 21 consolidation lesions with air bronchogram, and one of the ten cases demonstrated two lesions with airway dilatation. Three cases demonstrated 5 masses or nodular lesions, 3 of these 5 lesions showed air bronchogram. Two cases demonstrated 6 ground glass attenuation lesions. One case showed mediastinal and hilar lymphadenopathy. Conclusion Pulmonary MALT lymphoma usually appears as multiple bilateral consolidations, masses, nodules with air bronchogram or lesions with groundglass attenuation at CT imaging. The imaging findings described above and with an indolent clinical course may suggest the diagnosis of pulmonary MALT lymphoma.

Objective:To investigate the antimicrobial resistance spectrum of Escherichia coli isolated from different clinical sam-ples to guide the nosocomial infection control and rational use of antibiotics. Methods:Escherichia coli from clinical samples were isola-ted and tested by microbiological assay system of BD Phoenix 100 and susceptibility test card in 2013. The drug resistance was analyzed and compared retrospectively. Results: There were 936 strains of Escherichia coli isolated from our hospital in 2013. Escherichia coli was resistant to common antibiotics at high level. Escherichia coli strains isolated from different samples varied in the drug susceptibili-ty. The resistance rate of the strains from sputum samples was higher than that from the other samples. Conclusion:The status of drug resistance of clinical isolated Escherichia coli to antibiotics is very serious. Escherichia coli isolated from different samples show different drug resistant features, and the antimicrobial therapy should be considered according to the antimicrobial susceptibility tests.

The expressions of survivin and bcl-2 protiens were examined in 8 specimens from normal pituitary tissues and 38 pituitary adenomas by immunohistochemistry. The results showed that the expression of survivin was strongly associated with bcl-2 expression and abnormal expression of survivin resulted in inhibition of cell apoptosis, which might play a role in the pathogenesis of pituitary adenomas.

Objective To study the value of magnetic resonance imaging (MRI)in diagnosis of cubital tunnel syndrome (CuTS). Methods We studied the findings of electrophysiological examination and MRI in 30 elbows of 23 patients with CuTS and 15 elbows of 1 5 controls.We observed the motor conduction velocity (MCV),cross sectional area (CSA)and relative signal intensity (RSI)of the ulnar nerve acrossing the elbow at the point of largest size proximal to the entrapment point (CSA1 ,RSI1 )and the entrapment point (CSA2 ,RSI2 ).Then we calculated the ratio of CSA (CSAR=CSA1/CSA2 ),and the ratio of RSI (RSIR=RSI1/RSI2 ).Results The value of CSA1 and RSI1 was significantly greater than CSA2 ,RSI2 in the patient group (P<0.05).The value of CSA1 ,RSI1 , CSAR and RSIR in patients were significantly larger than that in controls (P<0.05).MCV was negatively correlated with CSA1 and CSAR in the patient group (r=-0.62,r=-0.53).There were no correlation between MCV and RSI1 ,RSIR in the patient group. The area under ROC curve of CSAR was the largest 0.94 (95% CI,0.83-1).The optimum cutoff point of CSAR was 1.83.The CSAR had sensitivity of 93.3% and specificity of 80% in diagnosis of CuTS.Conclusion MRI combined with the electrophysiologi-cal examination shows a high accuracy of locating the entrapment point of ulnar never lesion at the elbow.The CSAR of the ulnar nerve is the best MRI parameters in diagnosis of CuTS.

Purpose To analyze the hemodynamic characteristics of brain stem in patients with basilar artery hypoplasia (BAH) by magnetic resonance perfusion-weighted imaging (PWI).Materials and Methods According to the inclusion and exclusion criteria,51 patients with BAH were selected as the BAH group,and 79 patients without BAH were selected as the non BAH group.All patients were examined by MRI,3D-TOF and PWI,and magnetic resonance angiography was acquired after the three examinations.The regional cerebral blood flow (rCBF),regional cerebral blood volume (rCBV),regional mean transit time (rMTT) and time to peak (TTP) values of pontine area were measured.Results The rCBF value of the BAH group [(17.10±6.52) ml/(100 g · min)] was significantly lower than that of the non BAH group [(29.06± 13.32) ml/(100 g · min)] (P＜0.05);the rCBV value of the BAH group [(1.41 ±0.26) ml] was significantly lower than that of the non BAH group [(2.62± 0.82) ml] (P＜0.05);the TTP value of the BAH group [(6.14± 1.31) s] was significantly higher than that of the non BAH group [(5.39 ± 1.08) s] (P＜0.05);the rMTT value of the BAH group [(20.78±3.48) s] was significantly higher than that of the non BAH group [(19.01 ±2.39) s] (P＜0.05).TTP was the most sensitive index of cerebral perfusion injury,and the incidence of TTP extension was 41.18％ in the BAH group.Conclusion PWI can detect the abnormal cerebral hemodynamics in patients with BAH,which provides the basis for the timely treatment and prevention of irreversible injury in the ischemic area of the brain.