ObjectiveTo study the effect the low level laser irradiation in nostril combined with the electric stimulation of cerebellum on acute cerebral infarction.Methods88 patients with acute cerebral infarction were randomly divided into two groups, 45 cases in the study group who were accepted level laser irradiation in nostril combined with the electric stimulation of cerebellum and the normal medicine therapy, 43 cases in the control group who were accepted the normal medicine therapy only. The effects of two groups were observed.ResultsThe scores of the nervous function defect degree and the cerebrovascular dynamics indexes of study group were obviously increased (P<0.05 or P<0.01) compared with those before treatment. The indexes of blood viscosity were significantly decreased compared with those before treatment(P<0.01).However, all these indexes of the control group showed no significantly difference before and after treatment. ConclusionThe low level laser irradiation in nostril combined with the electric stimulation of cerebellum therapy is effective on acute cerebral infarction.It is helpful for early comprehensive recovery of motor function of the hemiplegic limbs.

OBJECTIVETo investigate the effect of anti-miR-145 on human airway smooth muscle cell (HASMC) proliferation and osteopontin systhesis in vitro and explore the mechanisms.

METHODSHASMCs were treated with 10-100 nmol/L anti-miR-145, and the cell proliferation and apoptosis were investigated using a CCK-8 assay and flow cytometry, respectively. The changes in osteopontin synthesis after the treatment was quantified with Western blotting.

RESULTSTreatment with 10 and 50 nmol/L anti-miR-145 significantly promoted the proliferation and osteopontin synthesis in HASMCs (P<0.05 or <0.01), and 50 nmol/L anti-miR-145 obviously inhibited the cell apoptosis (P<0.01).

CONCLUSIONAnti-miR-145 promotes HASMC proliferation and osteopontin synthesis and inhibits HASMC apoptosis in vitro, indicating the important role of anti-miR-145 in the pathogenesis of airway remodeling.

Airway Remodeling ; Apoptosis ; Cell Proliferation ; Cells, Cultured ; Humans ; MicroRNAs ; antagonists & inhibitors ; Myocytes, Smooth Muscle ; drug effects ; Osteopontin ; biosynthesis ; Respiratory System ; cytology