Objective To determine the association of-429T/C and G1704T polymorphisms in the receptor for advanced glycation end products gene with proliferative diabetic retinopathy (PDR).Methods Case-control study.From the Beijing Desheng Diabetic Eye Study cohort of 1467 patients with type 2 diabetes mellitus (T2DM),a total of 97 patients with PDR and 105 diabetic patients without retinopathy (DWR,duration of diabetes 15 years) were included for this study.Questionnaires were collected and general ophthalmologic examinations were performed.Biochemical analysis was conducted.DNA was extracted from peripheral venous blood.The-429T/C and G1704T single nucleotide polymorphisms were detected by the means of PCR-restrication fragment length polymorphisms.Results The frequency distribution of-429T/C in DWR group was 81.0％ in TT,16.1％ in TC,2.9％ in CC.The frequency distribution of-429T/C in PDR group was 77.3％ in TT,20.6％ in TC,2.1％ in CC.There was no significant statistical difference between the two groups (x2 =0.40,P＞0.05).Frequency of the-429T/C minor allele C in the DWR and PDR group were 11.0％ and 12.4％,respectively,with no significant statistical difference between the two groups (x2 =0.20,P＞0.05).The frequency distribution of G1704T in DWR group was 66.7％ in GG,29.5％ in GT,3.8％ in TT.The frequency distribution of G1704T in PDR group was 78.4％ in GG,21.6％ in GT.There was no significant statistical difference between the two groups (x2 =3.44,P＞0.05).Frequency of the G1704T minor allele T in the DWR and PDR group were 18.6 ％ and 10.8 ％,respectively,in which significant difference was found within the two groups (x2 =4.79,OR=1.88,95％CI:1.06-3.33,P＜0.05).Conclusions G1704T polymorphism is associated with PDR presence and 1704G allele may increase the risk of PDR.

Objective To investigate the intervention effect of herbal plaster on appetite loss of patients with gastrointestinal cancer undergoing chemotherapy.Methods Sixty-six patients treated in Department of Oncology, Affiliated Hospital of Nanjing University of Chinese Medicine from August 2015 to August 2016 were selected by convenient sampling, and were divided randomly into two groups. Patients in the control group were given conventional nursing, while patients in the observation group were given extra application of herbal plaster. The effect of intervention was evaluated by change of the patients' appetite and BMI.Results After completion of the treatment, appetite scores of patients in the observation group were (4.55±1.32) points, with that in the control group (2.22±1.43) (t=5.48,P<0.05); difference of BMI between the two groups showed no statistic significance (t=0.89,P>0.05).Conclusions Herbal plaster, as a characteristic technique in external treatment of traditional medicine, can help the patients regain their appetite to a certain extent and thus improve clinical outcomes. Still, clinical quantity-effect researches need to be strengthened in the future, so as to make the results more stringent, to better popularize and promote traditional Chinese nursing technology, and to provide new ideas and complementary therapy for clinical application of appetite loss caused by chemotherapy.