In recent years, neuro-electrophysiological techniques, as objective evaluation methods, have been gradually used in func-tional evaluation, guiding clinical rehabilitation programs and judging prognosis after stroke. They were proved to judge the integrity of cen-tral nervous system and conduction function and evaluate neuromuscular function. Nerve conduction, needle electromyography, H reflex, F wave, sympathetic skin response, motor-evoked potentials, somatosensory-evoked potentials, brain stem auditory-evoked potentials and sur-face electromyography were reviewed in this article.

BACKGROUND:The distance of spinal cord shifting backward after treatment through cervical posterior approach is affected by various factors. In fact, it is a morphological change after spinal cord is affected by external force.
OBJECTIVE:To analyze the influential factors for spinal cord shifting backward after posterior cervical decompression for cervical spondylotic myelopathy.
METHODS:Case data of 70 patients with multi-segmental cervical spondylotic myelopathy undergoing posterior cervical decompression were retrospectively analyzed. According to the different distances of spinal cord shifting backward after treatment, they were divided into three groups:<3 mm group, 3-5 mm group, and>5 mm group. In accordance with Japanese Orthopaedic Association Scores, the degree of cervical curvature and axial symptom index were compared.
RESULTS AND CONCLUSION:No significant difference in the recovery rate of Japanese Orthopaedic Association Scores was detected among the three groups. No significant difference in cervical curvature was visible among the three groups before and after treatment. The proportion of axial symptom was significantly higher in the patients of>5 mm group than the other two groups after treatment (P<0.05). These results suggested that the distance of spinal cord shifting backward was associated with axial symptom after treatment in patients with cervical spondylotic myelopathy after posterior cervical decompression.

In the paper, by taking acupuncture and migraine as the key words to retrieve CNKI and PubMed database, the literature analysis was done on the mechanism study on experimental migraine treated with acupuncture in rat model. The results showed that acupuncture mechanism study focused on the regulation and control of the relevant neurotransmitters/neuromodulators of migraine, such as calcitonin gene-related peptide (CGRP), serotonin (5-HT), nitric oxide (NO), etc. Moreover, in the paper, the review had been done on the neurotransmitters/neuromodulators involved in the study.
Acupuncture Points ; Acupuncture Therapy ; Animals ; Disease Models, Animal ; Humans ; Migraine Disorders ; genetics ; metabolism ; therapy ; Nitroglycerin ; metabolism ; Rats ; Serotonin ; metabolism

Objective: To investigate the chemical constituents in the dried whole plant of Lobelia chinensis. Methods: Column chromatography, such as silica gel, MCI, ODS, Sephadex LH-20, C18 reverse-phased silica gel columns, and preparative HPLC were used to isolate the compounds. Spectroscopic methods like MS, 1H-NMR, and 13C-NMR were used to elucidate their structures. Results: Fifteen compounds were isolated from 95% ethanol extract of L. chinensis, including twelve flavonoids: quercetin (1), rutin (2), luteolin (3), apigenin (4), hesperidin (5), quercetin-3-O-α-L-rhamnoside (6), quercetin-7-O-α-L-rhamnoside (7), quercetin-3- O-β-D-glucoside (8), amentoflavone (9), naringenin (10), hesperetin (11), and eupafolin (12), and three coumarins: 5, 7- dimethoxy-coumarin (13), isoscopoletin (14), and scoparone (15). Conclusion: Compounds 6-12 are isolated from the plants in genus Lobelia L. for the first time. Compounds 5, 13, and 14 are isolated from L. chinensis for the first time.

Objective To compare the therapeutic efficacy between conventional nursing and conventional nursing plus electroacupuncture in treating gastrointestinal dysfunction after abdominal operation, and their effects in recovering gastrointestinal function. Method Sixty patients with gastrointestinal dysfunction after abdominal operation were randomized into an electroacupuncture group and a control group according to their operation time, 30 cases in each group. The electroacupuncture group was intervened by conventional postoperative nursing plus electroacupuncture, by selecting bilateral Zusanli (ST36), Shangjuxu (ST37), Xiajuxu (ST39), Neiguan (PC6) and Taichong (LR3), connected to HANS electroacupuncture device, with frequency at 2 Hz and a tolerable intensity. The treatment was given once a day, for 5 d in total, till the recovery of gastrointestinal function. The control group was intervened by conventional postoperative nursing. The therapeutic efficacy was evaluated at the end of the intervention. The time of first bowel sound, first exhaust time, and first bowel movement time were observed. The gastrointestinal symptom score (GIS) was adopted to evaluate the effects of the two methods in improving gastrointestinal function at the end of the intervention. Result The general therapeutic efficacy of electroacupuncture group was better than that of the control group(P<0.05). In the electroacupuncture group, time taken for the first bowel sound, first exhaust and first bowel movement after the operation was shorter(P<0.05). The GIS dropped in both groups after the treatment, and the improvement was more significant in the electroacupuncture group(P<0.05). Conclusion Electroacupuncture helps promote the recovery of gastrointestinal function in patients with postoperative gastrointestinal dysfunction, and can produce a more significant improvement.

Objective To investigate the application effect of quality control management in the prevention of indwelling catheter associated urinary tract infection in intensive care unit (ICU) patients. Methods Cases of patients stayed in the department of ICU undergoing indwelling catheter over 10 d were selected by using time stage sampling method. Totally 136 cases of patients were selected from January 1, 2012 to December 31, 2012, as the control group. A total of 145 cases of patients were selected from January 1, 2013 to December 31, 2013, as the performance group. Cases in performance group were taken standardized training and quantify the performance appraisal on the basis of measures in the control group. The urine routine was tested and bacterial was cultured at indwelling catheter 3 d, 7 d and 10 d, respectively. The indwelling catheter associated urinary tract infections of the two groups were compared. Results After the implementation of the performance appraisal management, the incidences of indwelling catheter associated urinary tract infection at 3 d, 7 d and 10 d were 4.8%(7/145), 19.3% (28/145) and 32.4% (47/145), respectively. Within each quarter, the incidence of indwelling catheter associated urinary tract infection was rising with indwelling catheter time prolonged. And the incidences of the first and second quarter were higher than three and four quarter. The incidences of indwelling catheter associated urinary tract infections at 3 d, 7 d and 10 d in the performance group were lower than the control group, the differences were statistically significant (χ2=4.494, 30.660 and 49.307, P < 0.05). Conclusions Standardized training of nursing staff in ICU and implementation of performance appraisal management could effectively improve the enthusiasm and sense of responsibility of the nurses, and effectively reduce the incidence of indwelling catheter associated urinary tract infection.

Bone morphogenetic proteins (BMPs) are members of the transforming growth factor-β(TGF-β) superfamily and play critical roles in skeletal development, bone formation and stem cell differentiation.BMP9 is one of the most osteogenic BMPs, promoting osteogenesis differentiation of periodontal ligament stem cells ( PDLSCs) both in vitro and in vivo.Recently, signal transduc-tion studies have revealed that BMP-Receptor-Smads and BMP-Receptor-Mitogen activated protein kinase ( MAPK) play vital roles in BMP9 which promote PDLSCs osteogenesis differentiation.Several studies revealed that transcription factors closely associated with os-teogenesis differentiation are found in the downstream of the Smads and MAPK pathways.This review aims to summarize our current knowledge of BMP9-mediated osteogenesis by presenting recently completed works which may help us to further elucidate these path-ways.

Objective To observe the recovery efficiency of sufentanil and oxycodone hydrochloride in neurosurgical operation.Methods Total of 120 patients scheduled for microvascular decompression under general anesthesia were randomly divided into 4 groups to receive 0.03 mg·kg-1 oxycodone (group H1), 0.05 mg·kg-1 oxycodone (group H2), 0.08 mg·kg-1 oxycodone (group H3), and 0.08 μg·kg-1 sufentanil (group S).Each patient accepted 4 ng·mL-1 of remifentanil and 5-6 mg·kg-1·h-1 of propofol after induction to maintain bispectral index (BIS) value at 40-60.On starting to suture dura mater, oxycodone or sufentanil was given in various doses through intravenous route.The BIS value, the amount consumption of propofol, anesthesia and operation duration, and the drug delivery time of each group were recorded.The time of spontaneous breath recovery, awaking and extubation, verbal rating score (VRS) and sedation score (SS) at 10 min, 20 min, 30 min after operation as well as side-effects were also recorded.Results The VRS scores had no statistic difference among the groups at 10 min after operation, while the average scores of H1 were higher than those of H2 and H3 at 20, 30 min after operation.No statistic difference of the SS scores was showed among 4 groups at 10 min after operation, but it was lower in group H1 than those in group H3, and lower in group S than those in H2 at 20 min after operation;group H3 had a significantly higher SS scores than H1, H2 and S at 30 min after operation.Conclusion Oycodone hydrochloride 0.05 mg·kg-1 or sufentanil 0.08 μg·kg-1 can both provide satisfactory recovery quality in neurosurgical operation.

Ten novel conjugates with ursolic acid core and different chalcone ligands were synthesized via esteri-fication using the natural ursolic acid as starting material. The structures of these conjugates were confirmed by 1 H NMR, 13 C NMR and HRMS. The preliminary biological results showed that these compounds displayed signifi-cant antiproliferative effect on CNE2, KB, MCF-7, A549 and HepG2 cells. These compounds were more effective than ursolic acid and tamoxifen against MCF-7 cells. Especifically, compound 11e ( IC50 =4. 7 μmol/L) showed the greatest potency against MCF-7, which was about 3-times more potent than tamosifen ( IC50 =15. 2 μmol/L) . Additionally, all conjugates were nontoxic to health MCF-10A and VERO cells, and had higher security than tamoxifen.

OBJECTIVE:To investigate the relationship between therapeutic effects of N-acetylcysteine(NAC) on animals with fulminant hepatic failure and contents of plasma amino-acids METHODS:Plasma amino-acids were analysed as their precolumn derivatives with O-phthol-dialdehyde by reversed-phase high-performance liquid chromatographic separation and fluorescence detection RESULTS:NAC could decrease the contents of plasma amino-acids,increase the ratio of BCAA/AAA and decrease the mortality of fulminant hepatic failure animals CONCLUSION:Therapeutic effects of NAC on animals with fulminant hepatic failure were related to its effect on the contents of plasma amino-acids