Carcinoma in Situ ; metabolism ; pathology ; Carcinoma, Endometrioid ; metabolism ; pathology ; Cystadenocarcinoma, Serous ; metabolism ; pathology ; Endometrial Neoplasms ; metabolism ; pathology ; Female ; Humans ; Tumor Suppressor Protein p53 ; metabolism

High myopia is a complicated disease as it resembles with primary open-angle glaucoma(POAG)in appearance of the fundus.To avoid making mistake,we would think much of similarities and differences between them.Modern equipments give large number of informations about the fundus,which predominate to establish the quantitative analysis when following up other than early diagnosis.Visual field analysis is interfered by HM fundus dystrophy,also relationship with study curve,long wave motion and short wave motion.It is very important in diagnosis and differential diagnosis with routine methods in clinical work.The experience and judgment of the doctors are never replaced by equipment as yet.

Objective:To study the incidence,risk factors and clinical manifestaion for ectopic pregnancy(EP)after in vitro fertilization and embryo transfer(IVF-ET). Methods:A retrospective study of IVF and intracytoplasmic sperm injection (ICSI) pregnancies was performed between January 2002 and July 2005. Results:A total of 668 woman conceived after IVF or ICSI were studied. The rate of EP was 8.2%,corresponding with 55 Eps,of which 15 were heteropic pregnancy(HP). There were 40 Hps in tubal facter group,There apper to be no relationship between d2 ET and d 3 ET,ovulation induction and age. Conclusions:Tubal pathology is associated risk of EP.

Objective:To establish a new fluorescence-based quantitative PCR assay for detecting hepatitis B virus covalently closed circular DNA(HBV cccDNA).To explore the effect of artificial liver support system(ALSS)on total HBV DNA and HBV cccDNA in sera of patients with fulminant hepatitis B.Methods:The serum specimens from 50 patients of fulminant hepatitis B prior to and after treatment with ALSS were collected.Then HBV cccDNA was quantitatively detected by fluorescent PCR with specific primer set and Taqman probe.And the results were analyzed by SAS8.0 statistics software.Results:We successfully established a new fluorescence-based quantitative PCR method for HBV cccDNA.HBV cccDNA was detectable in sera of the patients with fulminant hepatitis B.The level of total HBV DNA and HBV cccDNA decreased significantly following plasma exchange in ALSS.The levels of total HBV DNA and HBV cccDNA had positive correlation with degree of PT,ALT,andAST.Total HBV DNA also had positive correlation with HBV cccDNA.Conclusion:The level of total HBV DNA and HBV cccDNA has positive correlation with degree of PT,ALT,and AST,which might be indication of hepatocyte damage.The finding that significant decrease in total HBV DNA and HBV cccDNA after plasma exchange in ALSS may provide a clue of further research about the fulminant hepatitis B treated with ALSS.

Objective:To investigate the outcomes,complications and safety about Endoscopic thoracic sympathectomy for palmar hyperhidrosis.Methods:Analyzing the results and complications about the 336 cases,who were received Endoscopic thoracic sympathectomy for treating palmar hyperhidrosis from Jan.2005 to Sep.2008,and were followed up.Results:All of their hands became dry and warm after operation.326 patiens were followed-up for 1 to 45 months,and 25.4 months on average.The total Results:No recurrence.Compensatory hyperhidrosis was found in 42 cases(12.88%).Conclusions:It is effective,safer and mini-trauma that Endoscopic thoracic sympathectomy for treating palmar hyperhidrosis.

Objective To analyze the results of human papillomavirus(HPV)DNA genotyping detection in 312 female outpatients attending STD clinics.Methods HPV DNA genotyping was detected by fluorescent quantitative polymerase chain reaction(FQ-PCR)for 312 samples collected from female outpatients attending STD clinics.Results The HPV detection rates in outpatients with HPV in their neoplasm and secretion were 83.8% and 38.1%,respectively,and the difference between the two groups had statistical significance.Of the 111 cases with visible neoplasm 4,5 and 57 cases were with 16,18 and 6/11 single type HPV infection,respectively,while 0,16,8 and 3 cases were.with 16+18,16+6/11,18+6/11 and 16+18/11 multiple type infection,respectively.Of the 201 cases with abnormal secretion including cases with other STDs,cases with CA and other STDs in their spouses,treated cases with CA,and cases asking for self-examination because of their risky sex behavior,15,12 and 43 were with 16,18,and 6/11 single type infection,respectively,while 2,5,1 and 0 cases were with 16+18,16+6/11,18+6/11,16+18+6/11 multiple type infection,respectively.Conclusions High HPV DNA rates were detected in neoplasm and secretion of female STD outpatients;although the low risk 6/11 type HPV infection was the main cause,the common high risk type HPV infection was also detected.Therefore,routine HPV DNA detection should be provided to this population group.

The research on war injuries in tropical areas is an important subject to be urgently dealt with at present in Chinese military medicine. Military medicine related to hot and humid environment is a significant branch of military environmental medicine. More efforts should be made in the research on care of war injuries as well as on military hygiene and military epidemiology so as to establish an integrated system involving basic and clinical science and art in military environmental medicine in hot and humid areas, which should be considered as an important task in the transition of our military strategy to win a war with improvement in the military medical support capability in a local high technicology conflict in such an area. In this paper, the significance of paying more attention to the research on the care of war injuries in hot and humid areas is emphasized, their features are highlighted, and main problems demanding further research are proposed.

Bone marrow mesenchymal stem cells(BMSCs) are a kind of non-hematopoietic cells living among bone marrow stroma,and are able to differentiate into mesoderm-type cells and non-mesoderm type cells.At present,there are three main methods for isolating and purifying BMSCs:bone marrow adherent culture,density gradient centrifugation and immune selection.This review listed many kinds of cells that BMSCs can differentiate into under certain conditions,discussed the related different directing conditions reported by researchers,analyzed the principle behind the artificial controlling conditions,and tried to find relatively uniform and effective directing conditions.The cells that BMSCs can differentiate into various types of cells including mesoderm-type cells,and non-mesoderm type cells:osteocytes,chondrocytes,adiocytes.As seed cells and a source of cell therapy in tissue engineering,BMSCs have been widely used in studying stem cells.

Tissue engineering has been one of the most challenging and rapidly developing frontier fields of science in the recent 20 years. Being referred to as “a medical revolution of far-reaching significance”, “a new epoch of regenerative medicine”, it signals the coming of a period of reproduction of regenerative tissue and organs. It has drawn intensive attention and support from medical circles and governments all over the world because of its advantages in trauma repair, functional reconstruction, life saving and improving quality of life, as well as its huge social and economic values. Products of tissue engineered skin and cartilage with approval of the American FDA have been available. Hundreds of tissue engineering companies and research centers have been set up both abroad and at home. It is now possible to construct tissue-engineered tissues. Moreover, projects of complex organ construction by means of tissue engineering is being carried out vigorously in the world. Some tissue-engineered tissues have been put into trial use in clinic and resulted in encouraging outcome. All these forecast a bright and promising prospect for tissue engineering. As a new branch of science and technology that involves multiple specialties and fields, however, it is still at its initial stage. Many fundamental scientific problems are to be explored, and many technical difficulties to be overcome. Hope and difficulty coexist; opportunity and challenge stand side by side. We should seize the opportunity and meet the challenge with our creative and strenuous efforts to make tissue engineering benefit human beings as early and much as possible.

PET is the most advanced nuclear medical imaging equipment. This paper introduces the characteristics, application and trend of PET.