Studies have proved that gastrin plays key roles in the development and progression of gastroenteric tumor. After gastrin combined with the tumor cell surface receptors, some genetic transcription and expression were adjusted through certain signal transduction pathways, resulting in the development and growth of gastrointestinal carcinoma. Effective treatment method would been founded about gastroenteric tumor through the studies of mechanism of action and signal transduction pathways of gastrin in gastroenteric tumor.

Objective To research the pattern distribution of TCM syndromes in upper airway cough syndrome(UACS)(source of the nose).Methods Two hundred cases of UACS(source of the nose) were observed to analyze the pattern distribution of TCM syndromes.Result The pattern distribution of TCM syndromes was as follows:wind-phlegm obstruction lung syndrome 76 cases(38%),wind-phlegm and stagnant heat syndrome 58 cases(29%),wind-cold and dampness syndrome 34 cases(17%),lung dry and Yin deficiency syndorme 32 cases(16%).Analysis of nose diseases history and TCM syndrome type showed that allergic rhinitis,chronic nasosinusitis and non-allergic rhinitis were main causes of UACS(source of the nose),account for 46.0%,25.5% and 14.5% respectively.Conclusion Wind-phlegm obstruction lung syndrome and wind-phlegm and stagnant heat syndrome were the most common syndromes of UACS.

Objective To study the expression of osteopontin (OPN) and collagen type Ⅰ in the rat myocardial fibroblasts infected by coxsackievirus B3 (CVB3) and to explore the possible pathogenesis of OPN on viral myocarditis.Methods Cardiac fibroblasts were isolated from neonatal rat and cultured with an traditional method.The primary cultured rat myocardial fibroblasts were infected by CVB3 which multiplicity of infection (MOI) was 0.5 PFU/cell.The myocardial fibroblasts were divided into control group 12 h and CVB3 groups (infected after 12 h,1 d,2 d,3 d).The expression of OPN and collagen type Ⅰ were detected by RT-PCR,Western Blotting and immunohistochemistry.And the linear correlation between the expression of OPN and the expression of collagen type Ⅰ was analyzed.Results ( 1 ) The gray scale values of the OPN/β-actin in control group(12 h)and viral groups (12 h,1 d,2 d,3 d) were 0.38 ± 0.06,0.56 ± 0.06,0.72 ± 0.05,0.98 ± 0.06,0.86 ± 0.02 respectively with RT-PCR,and were 0.26 ± 0.03,0.36 ± 0.03,0.52 ± 0.04,0.76 ± 0.05,0.62 ± 0.02 respectively with Western Blotting.The expression of OPN was found to be increased after 12 h infection,reached to the maximum after 2 d infection and displayed a decreased tendency after 3 d infection.There was significant difference on the gray scale values of the OPN/β3-actin ( F=74.965,53.004,respectively,P ＜ 0.05 ).(2) The gray scale values of the collagen type Ⅰ/β-actin in control group (12 h)and viral groups (12 h,1 d,2d,3 d) were 1.12 ± 0.03,1.18 ± 0.01,1.22 ± 0.02,1.33 ± 0.02,1.28 ± 0.03,respectively with RTPCR.These results suggested that the collagen type Ⅰ expression started to increase at 12 h when infected by CVB3,reached to the maximum at 2 d,and then decreased after 3 d infection,the difference was significant ( F =38.241,P ＜ 0.05).(3) The expression of OPN was positively correlated with the expression of collagen type Ⅰ ( r=0.948,P ＜ 0.001 ).Conclusion CVB3 can induce the expression of OPN and collagen type Ⅰ in the rat myocardial fibroblasts and the expression of OPN and collagen type Ⅰ displays positive correlation.It suggests that OPN can promote the expression of collagen type Ⅰ in myocardial fibroblasts,and may play an important role in the pathogenesis of viral myocarditis.

Humans ; Neoplasm Metastasis ; pathology ; Neoplasm Staging ; methods ; Neoplasms ; classification ; pathology ; therapy

Hepatitis, Autoimmune ; Humans ; Immunoglobulin G

Objective To evaluate the efficacy and toxicity of weekly docetaxel and cisplatin in previously untreated patients with advanced non-small-cell lung carcinoma. Methods Between January 2002 and December 2003 ,34 patients with pathologically comfirmed advanced non-small-cell lung carcinoma who had not received treatment were enrolled. The mean age was under 66 years. The patients received intravenous infusions of docetaxel(25 mg/m2,dayl ,8,15) with dexamethasone premedication and cisplatin(25 mg/m2,dayl ,8,15) ,followed by a week of rest. The remedies which were less than 6 regimens lasted to disease progression or severe toxicity. Therapeutic effect was evaluated by CT scan every two courses . The patients were followed up for 24 months. Descriptive statistics and SPSSIO. 0 software were used to analyse the results. Results 34 patients finished 90 courses. The mean was 2. 6 courses. All patients were followed up. Two patients achieved complete responses, ten patients achieved partial responses, ten patients achieved stable disease. An objective response rate of 35. 29% (95% confidence interval 19. 25%-51. 33% )was obtained. Patients life quality was significantly improved. The median time to progression was 4. 1 months, and median overall survival was 11 months. The 1-year survival rate was 47. 06% , the 2-year survival rate was 11.76% . Toxicities were mild. Grade 3 to 4 neutropenia (11.76%), anemia (5.88%), hyponatremia (5.88%), alopecie (17.64%) and nausea/vomiting (5. 88% ) were observed. Conclusion Weekly Cisplatin plus docetaxel is an effective and well-tolerated regimen in chemo-naive patients with advanced NSCLC. Well-designed clinical trials should be conducted.

Objective To explore the effects of penehyclidine hydrochloride (PHC) on lung epithelial type Ⅱcells against oxidative stress damage. Methods A549 cells treated with H2O2 were used as oxidative stress damage cell model. A549 cells were divided into 3 groups: control group (C group), H2O2 treated group (H group) and PHC treated group (P group). The viability of A549 cells was measured by MTT assay. The apoptotic rate was measured by TUNEL assay. The lev?els of malonicdialed (MDA), reactive oxygen species (SOD), reduced glutathione hormone (GSH) and nicotinamide adenine dinucleotide phosphate (NADPH) of cells were detected by biochemistry colorimetry. Results Compared with group C, the viability of A549 and the contents of SOD, GSH and NADPH were significantly decreased in group H, while MDA content and apoptotic rate were increased (P<0.05). Compared with group H, the viability of A549, the contents of SOD, GSH and NADPH were significantly increased in group P, while MDA content and apoptotic rate were reduced (P<0.05). Conclu?sion Penehyclidine hydrochloride shows protective effects on A549 cells through reducing the oxidative damage induced by H2O2.

In this paper,we summarize the teaching ideas,methods,and means for the course "Theory and Experiment of Molecular Biology" in which we teach students in an architecture-network-application way,exploring the education reform for the basic medical science.

Objective To analyse effects of zinc on immune function in children with infantile pneumonia .Methods A total of 96 children with infantile pneumonia selected from May to July 2014 were randomly divided into zinc supplementary treatment group and routine treatment group ,with 48 cases in each group .Routine treatment group was given conventional treatment ,while the zinc supplementary treatment group was received conventional treatment combined with zinc supplement .The serum level of zinc and immune function were detected before and after treatment ,respectively .Other 40 healthy infants received physical examination were contemporarily selected as healthy control group .Results Before treatment ,the levels of serum zinc ,IgA ,IgM and IgG ,percentages of CD3+ ,CD4+ cells and the CD4+ /CD8+ ratio in the two groups of children with infantile pneumonia were lower than those in the healthy control group ,while percentage of CD8+ cells was lower than that in the healthy control group ,had statistically significant differences(P<0 .05) .While no statistically significant differences of indicators mentioned above were found between the zinc sup‐plementary treatment group and routine treatment group before treatment(P> 0 .05) .After treatment ,the levels of serum zinc , IgA ,IgM and IgG ,percentages of CD3+ ,CD4+ cells and the CD4+ /CD8+ ratio in the zinc supplementary treatment group were higher than those in the routine treatment group ,and percentage of CD8+ cells was lower than that in the routine treatment group , had statistically significant differences(P<0 .05);while compared with the healthy control group ,levels of serum zinc ,IgA ,IgM , IgG were still lower ,had statistically significant differences(P<0 .05) .After treatment ,levels of serum zinc ,IgA ,IgM ,IgG ,per‐centages of CD3+ ,CD4+ cells and the CD4+ /CD8+ ratio in the routine treatment group were still lower than those in the healthy control group ,and percentage of CD8+ cells was still higher than that in the healthy control group ,had statistically significant differences(P<0 .05) .Conclusion Zinc can effectively improve the immune function of children with infantile pneumonia ,which might shorten treatment time and reduce adverse outcomes .

In the current situation,how to adapt to the new medical reform and determine its development goal has become the primary concern of the managers of No.1 Affiliated Hospital of Hebei North University.The internal and external conditions were analyzed by the method of SWOT and the advantages,disadvantages,opportunities and challenges facing the hospital were also observed.Finally,a series of countermeasures were put forward in accordance with the strategies by SWOT.