BACKGROUND:Extracorporeal shock wave (ESW) therapy is using principles of liquid-electric conversion and transfer to produce energy gradient difference and torsional tension in tissues with varied density.It is a matured technique in treating urinary and digestive system calculosis.However,the applications of ESW in treating nonunion or delayed osseous union are few.OBJECTIVE:To explore the therapeutic effects of ESW in treating nonunion or delayed osseous union by animal experiment and clinical verification.METHODS:The effect of ESW on periosteal metabolism was explored by focusing extracorporeal wave on bone tissues,and the autoradiography of H~(3-)TdR labeling rate was observed by animal experiment.ESW was used to treat nonunion patients (n=16) or delayed osseous union patients (n=16),and the follow-uP results were evaluated.RESULTS AND CONCLUSION:By autoradiography,the H~(3-)TdR labeling rate was greater in the experimental sides than that of the control sides at 1 and 2 weeks after operation (P＜0.05-0.01),which confirmed that the mitotic activity of periosteal osteoblasts was increased,namely,endosteal osteogenesis was enhanced.Clinical verification results demonstrated that,at 16 weeks after operation,16 patients with delayed union were all cured,13 were cured among the 16 cases of nonunion,and the remained 3 cases had notably callus formation.No adverse events occurred in all cases.The results suggested that it is reliable to treat nonunion or delayed osseous union using ESW therapy.

Biliary atresia is an infant disease characterized by obstruction of the internal and ex-ternal bile duct and progressive fibrosing process of the liver. Its etiology and pathogenesis remains un-clear and most infants would die in one year if thereˊs no medical intervention. In this article,we reviewed recent research progress of liver fibrosis in biliary atresia in infants and animal models,which proposed some unsettled problems and research direction in the future.

[Objective]To summarize the clinical experience of treating chloasma with the theory of the Zangfu-organs by Pro.Sun Yinong. [Method]By analyzing the idea of Sun’s therapy and medication of the proven case, it elaborates Pro.Sun Yinong’s clinical experience on using Chinese herbs to treat chloasma under the theory of the Zangfu-organs. [Results]The five Zang-organs perform their respective duties, any pathological changes in the five Zang-organs al can cause chloasma. We adapted at different herbs therapy to treat chloasma, which relieved the patient ’s clinical symptoms, and achieved satisfactory clinical outcome.[Conclusion] Starting from the five Zang-organs on the treatment based on syndrome differentiation, not only provides a new thought for the treatment of chloasma,but also ful y embodies the concept of holism and syndrome differentiation on TCM treatment of chloasma, which has practical effect on clinical application.

Breast Neoplasms ; complications ; therapy ; Female ; Humans ; Leukemia, Hairy Cell ; complications ; therapy ; Middle Aged ; Treatment Outcome

[Objective] To compare the efficacy of interbody fusion with cage versus corpectomy and fusion with plate in treating adjacent two-level cervical spondylosis.[Method] Sixty patients were included in this study.All patients were randomly assigned to anterior interbody fusion with cage (AIFC) group or anterior corpectomy and fusion with plate (ACFP) group. The average follow-up period was 28 months for AIFC group,and 25 months for ACFP group. [Result] The average loss of lordosis angle, the lost correction of segmental height and the scores on the criteria of 40-score method were not statistically significant between these 2 groups. The lordosis correction, operative estimated blood loss, and operative time were statistically better in the AIFC group significantly . [Conclusion] The short-term results of AIFC and ACFP in treating adjacent two-level cervical spondylosis were satisfactory. The advantages of AIFC are the elimination of donor site complications, increasing more segmental lordosis, and reducing blood loss and operative time.

Objective To evaluate the clinical results of the anterior cruciate ligament (ACL) reconstruction by bone-patellar tendon-bone(BPB) transplantation and fixation with absorbable interface screws under arthroscopy. Methods From December 1999 to June 2003, 32 patients with ACL injuries underwent BPB transplantation. There were 22 males and 10 females, and the average age was 32.5 years (ranged from 20-45 years). The lesions were left knees in 18 and right knees in 14, including 8 acute and 24 chronic injuries. The BPB for reconstructing the ACL was harvested from the anterior approach mini-invasively; the implants were fixed with the absorbable interface screws under arthroscopy. The associated injuries were treated simultaneously. All the patients were encouraged to rehabilitate with the continuous passive motion (CPM) and other physical therapies earlier after operation. Results All the patients were available at follow-up; the average duration of follow-up was 32 months (ranged from 6-40 months). Before operation, anterior drawer test positive in 32, Lachman test positive in 20 and pivot shift test positive in 16, were changed into negative. The patients were evaluated according to Lysholm knee scoring scale and the modified Lysholm knee score, the functional results of knee joint were classified as excellent in 24, good in 7 and fair in 1, and the average knee score was 52.1?5.6 and 98.7?3.6 before and after operation respectively, the difference was of statistical significance(P

Objective:To investigate the effect of TGF-? on the expression of type Ⅰ inositol 1,4,5-triphosphate receptor in WB rat liver epithelial cells.Methods:The expression of type Ⅰ inositol 1,4,5-triphosphate receptor protein and mRNA were detected by Western blot and RT-PCR in WB rat liver epithelial cells in vitro stimulating with TGF-?.Results:The expression level of type Ⅰ inositol 1,4,5-triphosphate receptor protein and mRNA in WB rat liver epithelial cells were both increased after stimulating with TGF-? in several time points and reached the highest at 8 h stimulated, and then decreased.Conclusion:TGF-? enhance the IP_3R1 protein and mRNA expression in WB rat liver epithelial cells.

3 ?g/well.All BALB/c mice immunized with pJME by im.and gene gun injection,and JE inactivated vaccines by ip.injection survived for 21 days.BALB/c mice immunized with pJE by im.injection and gene gun injection partially survived for 21 days.Titres of neutralization antibody produced with pJME were higher than pJE.Protective immunity and titre of neutralization antibody produced by im injection was the same as gene gun injection(im/gene gun injection: 1∶320/1∶320) at day 21.The antibody from BALB/c mice sera after twice pJME immunization only reacts with JEV-E protein. Conclusions Expression efficacy of proteins encoded by pJME and pJE in transfected cells is different.Expression level of related proteins was dependant on recombinant amount for transfection in a certain degree.Immunity effect induced with pJME was higher than pJE.The efficacy of DNA immunization produced by gene gun injection was higher than im.injection.Titres of neutralization antibody induced by DNA immunization were correlated to efficacy of protective immunity.Neutralization antibody from BALB/c mice sera produced by pJME immunization contained anti-E antibody against JEV-E protein.

[Objective]To investigate the effect of chondroitinase ABC(ChABC) after spinal cord injury(SCI) in adult rats,and to evaluate the significance of treatment. [Methods]Forty adult Wistar rats were divided into two groups: the control group(group A),the chondroitinase ABC group(group B).The thoracic T10 spinal cord injury model was made by impactor model II manual.After SCI,group B animals received ChABC treatment,while group A animals only received blank saline.The locomotor functions(BBB score) and the neuroelectrophysiological changes(SEP&MEP) of the rats were evaluated.Twelve weeks after SCI,biotinylated dextran amine(BDA) was injected into the cerebral cortex to trace the corticospinal tracts(CST).Then two weeks after the tracing,the animals were killed and the spinal cord frozen sections were made.The tissue sections were processed with Cy3 fluorescence stain,HE stain and anti NF-200 immuohistological stain.The section images were analyzed by Image Pro Plus image analyzing software.All the statistics were performed by SPSS 13.0 software.[Results]At 4 weeks after SCI,group B had obvious higher BBB scores than control group(P