With the development of the cross-correlation technique in surgery, the transplantation and vi-cariousness have become more and more important in repair surgery and reconstructive surgery. A nice bio-material could replace the related tissue or organ of human body, support the homologous function of organ-ism,and accelerate the process of self recovery and autotherapy. This review is about the status of research and application of biomaterial in surgery.

A nice biomaterial,which could replace the correlated tissue or organic of human body,supports the homologous function of organism,and accelerates the process of self recovery and autotherapy in the same time.The application of biomaterial in clinic,not only have the chemical characters and function of vitodynamics to match,but also be with nice biocompatibility and practical safety.Some development of biomaterial and the biocompatibility of it in this paper was reviewed,and the application status in the cardiothoracic surgery in recent years.

After the problems in digitalizing ancient books on traditional Chinese medicine were analyzed, the principles that should be followed in their digitalization were pointed out according to its theories and practice.The platform for digitalizing them was constructed with《Collective Notes to Canon of Materia Medica》as an example, and its design ideas, framework, development model and functional modules were described.

Objective:To discuss the quality control method of injection pump and analyze the factors that affect the quality control of injection pump.Methods: Flow error and blocking alarm error were tested by the INFUTEST 2000E dual-channel infusion pump analyzer according to the national calibration specifications.Results: The syringe specifications, injection speed and injection solvent could influence the test for accuracy of injection pump. The lower injection speed set, the bigger flow test error was, while the smaller the blocking alarm test error was. On the other hand, the higher the injection speed set, the smaller the flow test error was and the bigger the blocking alarm test error was. Besides, the higher qualified rate of flow test was, the lower the qualified rate of blocking alarm test was.Conclusion: The quality control of injection pump should abide by the principle of controllable risk, and should not pursuit too much accuracy.

Objective To study the diagnostic value of spiral CT pulmonary angiography(SCTPA) for pulmonary embolism(PE).Methods SCTPA scans were performed in 25 patients with PE,the contrast-enhanced SCT scans of the pulmonary arteries were performed,MPR,MIP and SSD were performed simultaneously.Results The 29 SCTPA scans were performed in 25 patients, including the central type in 23 cases(92%) and the peripheral type in 2 cases(8%).The rates of the main,the sinistral and dextral,the middle lobular,the lobular and the lingular pulmonary artery displayed were 100%,the rates of the segmental pulmonary artery displayed were 97.77% and the rates of the subsegmental pulmonary artery displayed were 68.00%.The lobular and segmental pulmonary artery were mainly embolized,the rates of PE were between 46.58% and 75.00%.Conclusion SCTPA has great value in earlier and clear and definite diagnosis of PE.

Adolescent ; Adult ; Automobile Driving ; China ; Electrocardiography ; Female ; Heart ; physiopathology ; Humans ; Male ; Middle Aged ; Noise, Occupational ; adverse effects ; Occupational Exposure ; adverse effects ; Stress, Psychological ; etiology ; physiopathology ; Vibration ; adverse effects

AIM:To explore the feasibility and biological characterization of long-term regulated expansion of JAK2 transduced human CD34+ cord blood cells in vitro.METHODS: A retrovirus (RV) vector which contains JAK2 catalytic domain and two binding sites for a chemical inducer, dimerization (AP20187), was cloned (designated MGI-F2JAK2). CD34+cells were enriched from cord blood with a MiniMACS system. The purified CD34+cells were transfected with supernatant from the retrovirus packaging cell line that expressed JAK2. Following transduction, cells were expanded into four groups: AP20187 alone, FL alone, TPO, alone, AP20187+FL+TPO, respectively. The expanded cells were monitored by GFP expression, immunophenotyping, progenitor colony assay, karyotype analysis as well as tumorigenesis in nude mice. RESULTS: The purity of selected CD34+ cells was over 91% and gene transfer rate was 49.32%?6.21%. Only the group of AP20187 +FL+ TPO was obtained a significant sustained outgrowth of the transduced CD34+ cord blood cells. The percentage of GFP+ cells consistently produced a rise to the 90% peak level by the end of 8th week of culture. Flow cytometry analysis showed that the phenotype of the expanded cells was CD33+, CD61+ and Gly-A+ partial positive; CD38+ and HLA-DR+ strong positive, while CD2, CD7 and CD19 were almost negative. Colony assays performed in methycelluos, which can give rise to BFU-E, CFU-GM and CFU-Mix, the CFU-GM was predominantly in all colonies. The tumor was not observed in nude mice and the karyotype analysis was normal from expanded cells.CONCLUSION: The results demonstrate that AP20187-mediated activation of JAK2 signaling is capable of stimulating expansion JAK2 transduced CB CD34+ cells in combination with FL and TPO. This system may have applications for studies in signaling transduction, hematopoiesis, and for gene and cell therapy.

BACKGROUND:A series of pathological changes were found in atlas, including atlas dislocation, atlanto-occipital junction instability and vertebral fractures. Atlantoaxial pedicle screw, nail and rod fixation or nail plate fixation is a novel repair method recently proposed, and has certain advantages. OBJECTIVE:To compare the repair effects of atlas pedicle screw on different types of atlas lesions. METHODS:A total of 42 fresh vertebrae were obtained from Department of Human Anatomy, Basic Medical School, Inner Mongolia Medical University. In accordance with bone thickness of sagittal vertebral artery groove bottom, vertebrae were divided into three types:type I (common type), type II (light variation), and type III (severe variation). Al samples were subjected to atlas pedicle screw fixation. RESULTS AND CONCLUSION:The screw pul out force in different bone densities of vertebrae was not the same. Vertebrae with great thickness and great bone density had big screw pul out force (P<0.05). However, the screw in different cortical bone of vertebrae had the same function. No compression on blood vessels or phenomenon affecting vertebral activity was visible. These results indicated that atlas pedicle screw implantation showed good safety, no side effects, did not affect the normal work and life of patients.

OBJECTIVETo investigate the prevalence and subtype distribution of human papillomavirus (HPV) infection and its correlation with age among women in Beijing urban area, and provide some epidemiological evidence for the clinical application of HPV vaccines.

METHODSWe collected cervical specimens from 1999 women in the Outpatient Department of our hospital, performed genetyping of HPV-DNA, and analyzed the incidence of HPV infection in different age groups.

RESULTSHPV infection was detected in 502 (25.2%) of the 1999 women patients, with 391 (19.6%) cases of high-risk HPV, which included 326 (83.4%, 326/391) cases of single infection. HPV-16 was the most common type (21.2%, 69/326), followed by HPV-52 (19.3%, 63/326) and HPV-58 (16.0%, 52/326). The prevalence of HPV infection was the highest among the women aged 41 -50 years and the lowest among those over 60 years.

CONCLUSIONThe subtype- and age-specific distribution of HPV infection among women in Beijing urban area shows an obvious heterogeneity, which deserves due consideration in the clinical application of HPV vaccines.

Adolescent ; Adult ; Age Distribution ; Aged ; China ; epidemiology ; Female ; Genotype ; Humans ; Middle Aged ; Papillomaviridae ; classification ; genetics ; Papillomavirus Infections ; epidemiology ; Young Adult