Background: Donor Human Milk (DHM) is the recommended food of infants whenever mom’s own milk (MOM) is not available. However, due to the pathogenic microbiological component of DHM, concerns on the safety of the milk are inevitable. Objective: To determine the effect of storage time on the microbial growth of pasteurized and unpasteurized Donor Human Milk maintained at a constant temperature of -20°C. Methodology: This is a Quasi-experimental Research done in the Newborn Care Unit (NCU) and Bacteriology Section of a private tertiary hospital in Davao City. The effect of storage time to the microbial growth of pasteurized and unpasteurized DHM was determined using Friedman Test 2-way Analysis of Variance by Ranks. Pairwise comparison of microbial growth between pasteurized and unpasteurized DHM at different storage times was determined using the Mann-Whitney U test. Results: Baseline DHM samples had moderately heavy bacterial growth of Staphylococcus epidermidis. There was a decrease from moderately heavy to light growth of the same species in the 24-hour storage time for both pasteurized and unpasteurized DHM. Pasteurized DHM did not have any microbial isolates at 48h, 72h, 4w, 8w and 12w while unpasteurized DHM had Acinetobacter baumanii, Staphylococcus warneri, Kocuria kristinae, and Staphylococcus saprophyticus growths. The analysis revealed that there is a statistically significant difference in the microbial growth in both pasteurized and unpasteurized DHM samples when stored at different times, χ2 (6) = 28.457, p = 0.00. Conclusions Storage time significantly interacts with the microbial growth on both pasteurized and unpasteurized DHM samples. Therefore, microbial growth in DHM samples may be affected by the length of time stored at a constant temperature of -20°C. Pasteurized DHM samples when stored at -20°C for more than 48 hours resulted to a statistically reduced microbial growth.
Milk, Human ; Pasteurization

BACKGROUND: Human milk is considered the optimal nutritional source for infants. Due to the possibility of microbial contamination during collection and handling, milk is pasteurized to prevent transmission of pathogens. In low-income areas where pasteurization is inaccessible, the pursuit for the best alternative in rendering donor milk safe remains.

OBJECTIVE: We aimed to determine and compare the efficacy of flash heat treatment and holder pasteurization in preserving human milk IgA while reducing bacterial contamination of donor breast milk.

METHODOLOGY: This is an experimental study which utilized pooled donor breastmilk from healthy mothers later subjected to bacterial analysis and immunoglobulin A level determination prior to and post flash heat treatment and pasteurization. Standardized scores were used to normalize population with unknown parameters. T-test comparison of means and Levene's test for equality of variances were used.

RESULTS: Twenty samples of aliquoted breastmilk were subjected to pasteurization and flash heat treatment, both yielding a statistically significant reduction in colony-forming units using Blood agar and MacConkey plates. These sample groups also underwent IgA level determination using Bindarid Kit IgATM and there was no significant decline in IgA levels.

CONCLUSIONS: Flash heat treatment may be an alternative for holder pasteurization in providing safe and effective breastmilk.

Measurement of the evoked action potential in muscle as an adjunctive to the clinical evaluation of peripheral nerve lesion resulted in the more frequent use of compound muscle action potential to evaluate peripheral nerve problems. Recently, the intraoperative use of nerve stimulation and recording technique have made it possible to evaluate the peripheral nerve problems intraoperatively. The present study was, therefore, undertaken to address this question in rabbit sciatic nerves and to determine intraoperative stimulation and recording technique are practical in clinical situations. A low-heat injury to the sciatic nerve was induced by perfusing 60degrees C saline around the nerve for 30 minutes and followed the courses of functional and morphological recovery of the nerve for 16 weeks. The results are ,summarized as follows ; In the test of compound muscle action potential(CMAP), the average amplitude and the onset latency were markedly attenuated at 4 and 8 week after the low-heat treatment (1.2mV, 4.2mV)(3.58msec, 2.68msec)(p=0.045, p=0.039). It progressively reverted to tile control level, showing 0.63 msec at 16 weeks. In the test of intraoperative nerve action potential(INAP), the average amplitude and the onset latency were attenuated at 4 and 8 weeks after the low-heat treatment(1.8mV, 2.1 mV)(1.18msec, 1.05msec)(p=0.041, p=0.043). There existed a significant positive correlation between the amplitude and onset latencies of INAP and CMAP measured in Low-heat group(r=0.67, r=0.71, p=0.003, p=0.009). Similar pattern of amplitude and onset latency between tests of CMAP and INAP suggests that INAP was practical and useful.
Action Potentials* ; Nerve Regeneration ; Pasteurization ; Peripheral Nerves ; Regeneration* ; Sciatic Nerve*

This study focused on the production of high quality compost for the growth of aero-thermophilic fungi, which has a promoting effect on the growth rate and production of oyster mushrooms. The automated solid-state bioreactor system was designed on the basis of a Three-Phase-One system, which controls the serial steps of prewetting, pasteurization and fermentation processes. High numbers of thermophilic fungi and bacteria were recovered from the mushroom composts prepared by this solid-state bioreactor. The rates of composting process were depended on physical as well as chemical factors. Among these factors, the parameters of moisture content and temperature were found to be particularly important. In our automated system, constant levels of moisture content, temperature and ventilation via mixing were provided by a centralized control apparatus including PLC, water tank and water jacket systems. These features induced higher microbiological activity of aero-thermophiles.
Agaricales ; Automation* ; Bacteria ; Bioreactors* ; Fermentation ; Fungi ; Ostreidae* ; Pasteurization ; Pleurotus* ; Soil* ; Ventilation ; Water

PURPOSE: For the best possible aesthetic reconstruction after craniofacial bone tumor resection, pasteurization has been adopted to devitalize neoplastic cells while maintaining osteoinductive properties and mechanical strength. This case report aims to demonstrate a long-term follow-up result of a monostotic fibrous dysplasia in frontal bone which was reconstructed by pasteurized tumoral autograft in situ. METHODS: A 14-year-old girl presented with a hard, nontender, slowly growing mass of 6-year duration on her left supraorbital area. CT showed 5 x 4 x 3 cm sized well defined bony mass confined to frontal bone with heterogeneous density. Tumor was excised completely through bicoronal approach and reimplanted to its original site after pasteurization at 60feminineC for 30 minutes. The pathologic examination confirmed fibrous dysplasia. RESULTS: She revisited our clinic 5 years later after suffering some assault on her face. On CT examination, pasteurized tumoral autograft was incorporated to host bone except the fractured upper orbital rim without any evidence of recurrence. She has been satisfied with the result. CONCLUSION: Pasteurization offers a simple, reliable, cosmetic, economic, and durable reconstruction method for craniofacial skeletal tumor. It has advantages of both biologic incorporation ability and mechanical strength without risk of recurrence. So, it should be considered as one of the primary options in benign as well as resectable malignant tumors of craniofacial skeleton.
Adolescent ; Cosmetics ; Fibrous Dysplasia, Monostotic ; Follow-Up Studies ; Frontal Bone ; Humans ; Orbit ; Pasteurization ; Recurrence ; Skeleton ; Stress, Psychological

The incidence of abdominal tuberculosis is decreasing due to more effective chemotherapy and pasteurization of milk and tuberculosis may nt be given serious consideration, especially when the primary pulmonary site is healed or not roentgenographically apparent. The clinical features of these infection are relatively nonspecific and the combination of abdominal mass, weight loss, anorexia and fever frequently lead to an erroneous primary diagnosis of neoplasm, especially lymphoma or carcinoma, metastatic to mesenteric lymph node. We have recently experienced a case of abdominal tuberculosis with huge tuberculous mesenteric lymphadenitis simulating neoplasm in 24 month old male. The case was reported with the brief review of literatures.
Anorexia ; Child, Preschool ; Diagnosis ; Drug Therapy ; Fever ; Humans ; Incidence ; Lymph Nodes ; Lymphoma ; Male ; Mesenteric Lymphadenitis* ; Milk ; Pasteurization ; Tuberculosis* ; Weight Loss

Human milk banks are a solution for mothers who cannot supply their own breast milk to their sick or hospitalized infants; premature infants, in particular, are unable to receive a full volume of breast milk for numerous reasons. As of December 2015, there was only one milk bank in a university hospital in Korea. We reviewed the basic characteristics of donors and recipients, and the amounts and contamination of breast milk donated at the Human Milk Bank in Kyung Hee University Hospital at Gangdong in Korea from 2008 to 2015. The donor pool consisted of 463 first-time donors and 452 repeat donors who made 1,724 donations. A total of 10,820 L of breast milk was collected, and 9,541.6 L were processed. Detectable bacteria grew in 12.6% after pasteurization and 52.5% had cytomegalovirus DNA before pasteurization in donated milk. There were 836 infant and 25 adult recipients; among new infant recipients, 48.5% were preterm; the groups received 8,009 and 165.7 L of donor milk, respectively. There was an increase in the percentage of preterm infants among new infant recipients in 2015 (93.1%) compared to 2008 (8.5%). Based on the number of premature infants in Korea, the number of potential recipients is not likely to diminish anytime soon, despite efforts to improve the breastfeeding rate. Sustainability and quality improvement of the milk bank need long-term financial support by health authorities and a nationwide network similar to blood banking will further contribute to the progress of milk banking.
Adult ; Bacteria ; Blood Banks ; Breast Feeding ; Cytomegalovirus ; DNA ; Financial Support ; Humans* ; Infant ; Infant, Newborn ; Infant, Premature ; Korea ; Milk ; Milk Banks ; Milk, Human* ; Mothers ; Pasteurization ; Quality Improvement ; Tissue Donors

PURPOSE: The purpose of this study is to describe the rate of cytomegalovirus (CMV) virolactia, and the prevalence of breast milk (BM)-transmitted postnatal CMV infection among premature infants after freeze-thawing (FT) and Holder pasteurization (HP) of breast milk. METHODS: This is a single-center, retrospective study of 312 infants born at less than 32 weeks of gestation, or with a birth weight less than 1,500 g from January 2013 to June 2017. All infants were screened for CMV-specific immunoglobulin (Ig) G and IgM at birth. Initial CMV specific polymerase chain reaction (PCR) and CMV culture were performed on mothers' BM and babies' urine within the first 21 days of life. FT and HP of BM was used to prevent the transmission of CMV. For the surveillance of postnatal CMV infection, CMV culture and CMV specific PCR of urine from babies were repeated one to two months after the initial screening. Screening for viremia and viruria was performed if postnatal CMV infection was suspected. RESULTS: Among 178 BM samples obtained from mothers of CMV-IgG-seropositive infants, 80 (44.9%) were CMV PCR positive. CMV deoxyribonucleic acid (DNA) was detected in five of the 22 BM samples (22.7%) obtained from the mothers of CMV-IgG seronegative infants. When CMV DNA load in BM was measured before and after HP, various results were shown. Sixty-three infants out of 232 (27.2%) were evaluated for postnatal CMV infection and four infants out of 63 (6.3%) were infected. CONCLUSION: Interventions to prevent BM-transmitted CMV infection can reduce the chance of postnatal CMV infection, but not completely eliminate it.
Birth Weight ; Breast* ; Cytomegalovirus Infections* ; Cytomegalovirus* ; DNA ; Humans ; Immunoglobulin M ; Immunoglobulins ; Infant ; Infant, Newborn ; Infant, Premature* ; Mass Screening ; Milk, Human ; Mothers ; Parturition ; Pasteurization ; Polymerase Chain Reaction ; Pregnancy ; Prevalence ; Retrospective Studies ; Viremia