1.Advances in research on biology of B19 virus.
Xue-Li LIU ; Min WANG ; Zhuo-Zhuang LU ; Tao HONG
Chinese Journal of Virology 2011;27(6):599-603
2.Research advance in human bocavirus.
Chinese Journal of Contemporary Pediatrics 2010;12(8):678-680
3.Characteristics of human bocavirus infection in children in Chongqing area.
Yuan DING ; Xiao-dong ZHAO ; Xin CHEN
Chinese Journal of Pediatrics 2009;47(6):466-467
Child
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China
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epidemiology
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Female
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Human bocavirus
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Humans
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Infant
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Infant, Newborn
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Male
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Parvoviridae Infections
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epidemiology
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virology
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Respiratory Tract Infections
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epidemiology
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virology
4.Advances in Parvovirus Non-structural Protein NS1 Induced Apoptosis.
Mengyu TU ; Fei LIU ; Shun CHEN ; Mingshu WANG ; Anchun CHENG
Chinese Journal of Virology 2015;31(6):679-684
Until now, more than seventeen parvovirus have been reported which can infect mammals and poultries. The infected cells appeared different properties of apoptosis and death, present a typical cytopathic effect. NS1 is a major nonstructural protein of parvovirus, with a conservative structure and function, which plays an important role in the viral life cycle. In addition to the influence on viral replication, the NS1 also participates in apoptosis induced by viruses. Parvovirus induced apoptosis which is mainly mediated by mitochondrial pathway, this review summarized the latest research progresses of parvovirus induced apoptosis.
Animals
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Apoptosis
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Humans
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Parvoviridae Infections
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physiopathology
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veterinary
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virology
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Parvovirus
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genetics
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metabolism
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Viral Nonstructural Proteins
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genetics
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metabolism
5.Research advances in porcine bocavirus.
Shao-Lun ZHAI ; Sheng-Nan CHEN ; Wen-Kang WEI
Chinese Journal of Virology 2012;28(2):190-193
Porcine bocavirus (PBoV) was considered as a new member of the genus Bocavirus of the subfamily Parvovirinae of the family Parvoviridae, which was discovered in Swedish swine herds with postweaning multisystemic wasting syndrome (PMWS) in 2009. At present, as an emerging pathogen, it was paid great attention by researchers at home and abroad. This paper referred to some published literatures and reviewed several aspects of PBoV including its finding, classification, genome structure and replication, epidemiology, associativity with diseases, cultural and diagnostic methods.
Animals
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Biomedical Research
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Bocavirus
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classification
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genetics
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isolation & purification
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physiology
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Parvoviridae Infections
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diagnosis
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veterinary
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virology
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Swine
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Swine Diseases
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diagnosis
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virology
6.Sequence the complete sequence of bocavirus I with SISPA-PCR.
Jin-Song LI ; Wei-Xia CHENG ; Dong-Ping YAO ; Bei LAN ; Jie-Mei YU ; Yan LIU ; Yong-Qing LI ; Qing ZHANG ; Miao JIN ; Zi-Qian XU ; Dan-Di LI ; Zhao-Jun DUAN
Chinese Journal of Experimental and Clinical Virology 2010;24(1):14-16
OBJECTIVETo sequence the complete sequence of bocavirus I with sequence independent single primer amplification (SISPA-PCR).
METHODSTo exclude the co-effection samples, all clinical samples of diarrhea cases were screened with special primers of rotavirus, astrovirus, adenovirus, calicivirus and bocavirus I. The virus were enriched through ultracentrifugation. Other nucleic acids, such as human and bacteria genomes, were degradated by DNase I and RNase. DNA of bocavirus was Amplificated with SISPA-PCR, then purificated, cloned and sequenced. The sequences were alighmented in nr with blastn and assembled with DNAstar.
RESULTSA 4834bp sequence of bocavirus I were assembled.
CONCLUSIONSISPA-PCR is an economical and efficient technique for sequence a virus complete genome.
Base Sequence ; Bocavirus ; genetics ; isolation & purification ; DNA Primers ; genetics ; Diarrhea ; virology ; Genome, Viral ; Humans ; Parvoviridae Infections ; virology ; Polymerase Chain Reaction
8.Discovery of human bocavirus 4 in children with acute gastroenteritis in Lanzhou, China.
Jingyao XIANG ; Xin MA ; Yanqing GUO ; Zhaojun DUAN ; Yuning LI ; Dandi LI
Chinese Journal of Virology 2015;31(1):42-45
We studied the epidemiological characteristics of human bocavirus 4 (HBoV4) in children with a- cute gastroenteritis in Lanzhou (China). A total of 331 stool specimens were collected from children aged < 5 years with acute diarrhea at the First Hospital of Lanzhou University between July 2012 and June 2013. Specimens of HBoV were identified by nested polymerase chain reaction assays. Compared with related sequences in GenBank, the HBoV-positive strain isolated in the present study was,quite surprisingly, a rare genotype named HBoV4. This strain was a typical HBoV4,with high levels of nucleotide and amino acid homology to the Thailand strain, JQ267789 (98.9% and 98.7%, respectively), and the USA strain, GQ506568 (97.6% and 97.4%, respectively). This is the first report of HBoV4 as the causative agent for acute gastroenteritis in pediatric patients in China. This strain is one of two genotypes of HBoV that are currently circulating.
Child, Preschool
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China
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Feces
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virology
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Female
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Gastroenteritis
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virology
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Human bocavirus
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classification
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genetics
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isolation & purification
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Humans
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Infant
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Male
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Molecular Sequence Data
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Parvoviridae Infections
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virology
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Phylogeny
9.Etiological study of human bocavirus 1-4 in children with acute diarrhea in Lanzhou, China.
Jing-Yao XIANG ; Dan-Di LI ; Xin MA ; Yan-Qing GUO ; Zhao-Jun DUAN ; Yu-Ning LI
Chinese Journal of Virology 2014;30(4):402-407
This study aimed to study the epidemiological and clinical characteristics of human bocavirus 1-4 (HBoV1-4) in children with acute diarrhea in Lanzhou and to investigate the association between HBoV and acute gastroenteritis. A total of 331 stool samples were collected from children aged under 5 years with acute diarrhea at the Department of Pediatrics, the First Hospital, Lanzhou University, between July 2012 and June 2013. Nested PCR was used to screen for HBoV and a general PCR was employed to screen other common diarrhea viruses. We found human bocavirus 1, 2, 3 and 4 in 26, 15, 7 and 1 cases, respectively. There was no specific seasonal distribution of HBoV, with infections occurring throughout the year. HBoV was mostly found in children aged between 7 and 12 months, with a mean age of 11.04 months (+/- 6.92 months), and 93.88% of affected children were aged under 2 years. Overall, 71.3% of mixed infections were mixed and the majority of other infections were caused by rotavirus. There was no statistical difference in the incidence of fever and vomiting associated with HBoV infection. A rare virus strain, HBoV4 (LZFB086), was identified, which showed highest levels of nucleotide sequence identity (99.0%) with a single Thai HBoV strain (JQ267789). No case of HBoV2B was found. In conclusion, HBoV1 was a major etiological pathogen of HBoV in pediatric cases in Lanzhou. HBoV4 was detected in feces for the first time in China. The rate of mixed infections was high and rotavirus was dominant. The data presented suggests that HBoV is not a major causative agent of gastroenteritis.
China
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epidemiology
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Diarrhea
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epidemiology
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virology
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Feces
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virology
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Human bocavirus
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classification
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genetics
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isolation & purification
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Humans
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Infant
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Molecular Sequence Data
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Parvoviridae Infections
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epidemiology
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virology
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Phylogeny
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Seasons
10.The correlational study of viral load of human bocavirus and clinical features in children with acute respiratory tract infection.
Li-hua ZHU ; Hui-qing XU ; Shi-rong QI ; Ji-shan ZHENG ; Ya-ping CHEN ; Li-li CHEN
Chinese Journal of Experimental and Clinical Virology 2011;25(4):271-273
OBJECTIVETo analyze the correlations between clinical features in paediatric patients with acute respiratory tract infection (ARTI) and viral load of human bocavirus.
METHODSA prospective study was conducted on 956 children < 5 years admitted with an acute respiratory tract infection from November 2009 to December 2010, and 251 healthy children conclused as control group in the corresponding period. Human bocavirus was investigated in nasopharyngeal aspirates (NPA) and throat swab by PCR, and viral load was detected by real-time polymerase chain reaction (RT-PCR) in HBoV positive sample. Clinical data were also prospectively recorded.
RESULTSA significant difference was found in HBoV positive rate between children with ARTI and control group at enrollment. There was a significant difference in HBoV viral load between children with upper respiratory tract infection and lower respiratory tract infection. HBoV viral load did not differ significantly between children with upper respiratory tract infection and control group. Among children with lower respiratory tract infection, no significant difference were detected between common and severe cases in HBoV viral load. HBoV viral load did not differ significantly whether the children were with or without co-infection.
CONCLUSIONSHBoV could be detected perennial and considered as a major pathogen associated with acute respiratory tract infection in children. However, HBoV may not be a independent factor in children with ARTI and the HBoV viral load was not associated with the severity of respiratory illness.
Case-Control Studies ; Child, Preschool ; Female ; Human bocavirus ; genetics ; isolation & purification ; physiology ; Humans ; Infant ; Male ; Parvoviridae Infections ; virology ; Respiratory Tract Infections ; virology ; Viral Load