The authors reported a PCR-based protocol for the detection of Human papilloma virus in vaginal swab speci
Papilloma ; Papilloma/virology ; Diagnosis

OBJECTIVE: To evaluate the relationship between human papilloma virus (HPV) infection and the occurrence, recurrence and malignant transformation of nasal inverted papilloma (NIP). METHOD: With comprehensive retrieval of related literature that had been published in databases included Pubmed (1990--2011), Cochrane Library, CNKI (1979-2011), VIP (1989-2011), CBM (1990-2011) and WANFANG Meta-analysis software Rev-Man 5.0 was used to analyze the raw data and to calculate the value of combined odd ratio (OR) and 95% confidence interval (CI). RESULT: According to Meta-analysis, the occurrence group between HPV and NIP, the OR was 34.44 and 95% CI was 12.96-91.56; the recurrence group, the OR was 3.66 and 95% CI was 1.77-7.56, to the high-risk HPV, the OR was 1.94 and 95% CI was 0.30-12.58; the malignant transformation group, the OR was 1.79 and 95% CI was 0.94-3.40 to the high-risk HPV, the OR was 49.35 and 95% (CI was 0.45-11.23. CONCLUSION HPV may play an important role in the occurrence and recurrence of NIP and high-risk HPV was closely related to the progress of NIP.
DNA, Viral ; Humans ; Nose Neoplasms ; virology ; Papilloma, Inverted ; virology ; Papillomaviridae ; Papillomavirus Infections ; pathology

OBJECTIVETo investigate the presence of HPV infection of oral mucosa proliferative lesions in children and determine the associations of HPV types with oral mucosa lesions in children.

METHODSImmunohistochemical method and in situ hybridization techniques were applied to detect human papillomavirus (HPV) infection in biopsies taken from clinical lesions in oral mucosa of 30 children.

RESULTSThe most frequent lesions detected were SCP (66.7%), followed by CA and FEH. The HPV viral antigen was present in 73.3% (22/30) of the oral benign epithelial proliferative lesions in children. A high frequency HPV was found in CA (6/6) and SCP (15/20) by means of IHC. In the ISH positive case, high risk HPV 16/18 was observed in 77.3% (17/22).

CONCLUSIONThis study demonstrates a high prevalence of HPV infection in children's oral mucosa proliferative lesions, and high-risk HPV16/18 are predominant in children's oral mucosa proliferative lesions.

Adolescent ; Child ; Child, Preschool ; Condylomata Acuminata ; virology ; DNA, Viral ; analysis ; Female ; Focal Epithelial Hyperplasia ; virology ; Humans ; Immunohistochemistry ; Infant ; Male ; Mouth Mucosa ; virology ; Papilloma ; virology ; Papillomaviridae ; isolation & purification

OBJECTIVETo detected HPV human papillomavirus DNA and HPV types in NIP nasal inverted papilloma, to inquire into the infection of HPV in the pathogenesis of NIP, and the relationship of HPV with the prognosis of NIP.

METHODSTwenty-eight cases of NIP were divided into 3 groups: no recurrence group (group 1), recurrence group (group 2), squamous cell carcinoma (SCC) arising in NIP group (malignancy group, group 3). Ten cases of benign nasal polyps were used as control group. HPV-DNA types of 6, 11, 16, 18 and general type were detected by polymerase chain reaction (PCR).

RESULTSTotal positive rate of HPV in NIP was 75% (21/28). The positive rate of group 1 was 42% (5/12), all with single and low risk HPV type infection (4 with HPV6 and 1 with HPV11). Thirteen cases of recurrence and 3 cases of malignancy were discovered to have HPV-DNA, in group 2, the majority were HPV6 and HPV11, 4 cases with double infection;in group 3, the majority were HPV16 and HPV18, and 2 cases with double infection.

CONCLUSIONSThe occurrence of NIP was related with HPV infection. To detected HPV and its subtypes can show the cases easily to have recurrence or malignant change. For the cases with HPV positive, double infection and infected with high risk types should be closely followed-up.

Case-Control Studies ; Humans ; Nose Neoplasms ; diagnosis ; virology ; Papilloma, Inverted ; diagnosis ; virology ; Papillomaviridae ; classification ; genetics ; Papillomavirus Infections ; diagnosis ; virology ; Paranasal Sinus Neoplasms ; diagnosis ; virology ; Prognosis

OBJECTIVE: To investigate the correlation of laryngeal squamous cell carcinoma (LSCC) and precancerous lesion with HPV infection subtypes and possible clinical relationship. METHOD: Eighty-three cases in paraffin embedded tissues were detected with thirty seven HPV subtypes by flow-through hybridization and gene chip (HybriMax), including 31 cases of laryngeal squamous cell carcinoma, 52 cases of precancerous lesions (29 cases of vocal cord leukoplakia and 23 cases of laryngeal papilloma), and 36 cases of vocal cord polyp as normal vocal mucosa were used as control. RESULT: The total positive rate of HPV was 19.4% in the group of laryngeal squamous cell carcinoma (6/31), 0 in vocal cord leukoplakia, 65.2% in laryngeal papilloma (15/23), and the control group were all negative, HPV virus subtype of HPV-positive laryngeal squamous cell carcinoma were all high-risk HPV16; and there were 6 HPV virus subtypes in laryngeal papilloma (8: HPV6,4: HPV52, 1: HPV11, 1: HPV18, 2: HPV45, 3: HPV16), individual mixing two or more subtypes infection. HPV infection of laryngeal squamous cell carcinoma and precancerous lesions has no statistically significant difference according to gender, high low-risk subtypes. CONCLUSION HPV infection related to laryngeal squamous cell carcinoma and precancerous lesions, but no significant correlation with the subtype distribution of high and low risk; HPV detection is making positive sense to clinical diagnosis of laryngeal carcinoma and precancerous lesions as well as the development of specific HPV subtype vaccine.
Carcinoma, Squamous Cell ; complications ; virology ; Genotype ; Head and Neck Neoplasms ; complications ; virology ; Human papillomavirus 11 ; Humans ; Laryngeal Neoplasms ; complications ; virology ; Papilloma ; complications ; virology ; Papillomaviridae ; classification ; Papillomavirus Infections ; complications ; virology ; Precancerous Conditions ; Squamous Cell Carcinoma of Head and Neck

OBJECTIVETo study the distribution of human papillomavirus (HPV) subtypes in nasal inverted papilloma (NIP), and to evaluate the relationship between HPV and NIP.

METHODSTwenty-one HPV subtypes were detected in paraffin-embedded tissues of 101 cases of NIP by flow through hybridization and gene chip (HybriMax), 24 cases of normal nasal mucosa were used as controls.

RESULTSHPV positive rates of NIP were 64.36% (65/101). Benign NIP group, NIP with atypical hyperplasia group, NIP with cancerous group of HPV positive rates were 59.7% (46/77), 81.8% (18/22) and 50% (1/2) respectively. The control group was negative (0/24). The comparison between NIP group and control group was statistically significant (chi(2) = 32.178, P < 0.05). Benign NIP group and NIP with atypical hyperplasia group were compared, but no statistically significance (chi(2) = 3.649, P = 0.056) was found. The constituent ratio of benign NIP group and NIP with atypical hyperplasia group in high, low-risk HPV subtypes infections was compared, a statistically significance (chi(2) = 10.412, P < 0.05) was found.

CONCLUSIONSThe occurrence of NIP was related with HPV infection. High-risk HPV subtype infections or multiple infections will prompt benign NIP to NIP with atypical hyperplasia. Understanding the distribution of HPV subtypes in the NIP is helpful to predict the clinical behavior.

Adult ; Aged ; Case-Control Studies ; Female ; Humans ; Male ; Middle Aged ; Nose Neoplasms ; pathology ; virology ; Papilloma, Inverted ; pathology ; virology ; Papillomaviridae ; classification ; Papillomavirus Infections ; pathology

We reported a case of an African American woman who went to the hospital with palpable right breast lump with bloody nipple discharge at University of Texas Medical Branch at Galveston. The modalities of breast imagings included mammography and ultrasonography. The method used for viral identification was Linear Array HPV genotyping test. Intraductal papilloma revealed as high density tubular or rounded lobular masses with partially circumscribed, obscured margins and clustered punctate microcalcifications on mammograms. Ultrasound showed as intraductal masses with dilated ducts. The core biopsy demonstrated duct filled with papillary lesion and post excision revealed intraductal papilloma. HPV DNA types 16, 33, 58 and 71 were detected after use of Linear Array HPV genotyping test.
Breast Neoplasms ; diagnostic imaging ; pathology ; virology ; Female ; Genotype ; Humans ; Mammography ; Middle Aged ; Papilloma, Intraductal ; diagnostic imaging ; pathology ; virology ; Papillomaviridae ; classification ; genetics ; isolation & purification

OBJECTIVE: To investigate the relationship between human papillomavirus (HPV) infection and pathogenesis of nasal inverted papilloma (NIP) and its malignant transformation. METHOD: Fifty-seven cases of NIP were divided into 2 groups: benign group, squamous cell carcinoma (SCC) arising in NIP group (malignancy group). HPV-DNA types of 6, 11, 16, 18 were detected by polymerase chain reaction (PCR) in 57 cases of NIP. Thirty cases of nasal polyps were control. RESULT: Total positive rate of HPV in NIP was 64.9% (37/57). The positive rate of benign group was 60% (27/45), all with single and low risk HPV11 type infection. The positive rate of malignancy group were was 83.3% (10/12), and the majority were HPV16 and HPV18. Five cases had double infection (4 with HPV16 and HPV 18, 1 with HPV11 and HPV18), four had single HPV16 infection, another one had single HPV11 infection. However, HPV-DNA was not detected in any cases of nasal polyps. CONCLUSION Infection of HPV-DNA has an important effect in pathogenesis of NIP. Meanwhile, there maybe a close relationship between high risk HPV16 type and HPV18 type and malignant transformation of NIP.
Adult ; Aged ; Aged, 80 and over ; Cell Transformation, Neoplastic ; pathology ; Female ; Humans ; Male ; Middle Aged ; Nose Neoplasms ; pathology ; virology ; Papilloma, Inverted ; pathology ; virology ; Papillomaviridae ; Papillomavirus Infections ; pathology ; Young Adult

OBJECTIVETo compare the molecular basis difference between recurrent respiratory papillomatosis (RRP) and vocal cord polyp, to analyze the expression of glycan structural genes, and to discuss the pathopoiesis mechanism of RRP.

METHODSThe gene expressing profile between the 3 groups papilloma and the vocal cord polyp regarded as normal larynx epithelium were compared using mRNA parallel amplify and the human genome gene expressing microarray. Through cluster analysis, Gene Ontology function gene annotation and path way analysis, the relative gene of RRP and HPV infection were acquired.

RESULTSAccording to three microarrays results, total 567 expression changed genes related to HPV induce RRP were acquired. A serial change of glycan structure biosynthesis and degradation pathways was significant. The expression of dolichyl-phosphate mannosyltransferase polypeptide 1 (DPM1), asparagine-linked glycosylation 1 homolog (ALG1), fucosyltransferase 8 (FUT8) and alpha-mannosidase 1A (MAN1A) were regulated and beta-hexosaminidase (HEXB), beta1-galactosidase (GLB1), exostoses 1 (EXT1), fucosyltransferase (FUT) reduced expression and heparan sulfate 3-O-sulfotransferase 1 (HS3ST3A1) increased expression. The two related enzymes of the glycosphingolipids which is the main composed of the cell membrane, beta-3-N-acetylglucosaminyltransferase 4 (B3GNT4) and UDP-glucose ceramide glucosyltransferase (UGCG) increase expression, HEXB and GLB1 reduced expression.

CONCLUSIONSThe alteration of the coding genes of glycan structure biosynthesis and degradation pathways were significantly and characteristically in pathopoiesis mechanism of RRP. This abnormality may be the beginning of tumor form HPV infection.

Adult ; Gene Expression Profiling ; Glycolipids ; genetics ; Glycoproteins ; genetics ; Humans ; Laryngeal Neoplasms ; genetics ; pathology ; virology ; Oligoribonucleotides ; genetics ; Papilloma ; genetics ; pathology ; virology ; Papillomaviridae ; genetics ; Polyps ; genetics ; pathology ; virology ; Respiratory Tract Neoplasms ; genetics ; pathology ; virology ; Vocal Cords ; pathology

OBJECTIVE: To investigate the significance of expression of COX-2, p21, Ki67 and HPV in nasal inverted papilloma. METHOD: Detecting COX-2, p21, Ki-67 in 30 cases of nasal inverted papilloma (NIP), 20 cases of nasal polyps (NP) and 10 cases of normal nasal mucosa (NM) by two step immunohistochemical method, and HPV virus by flow-through hybridization method. RESULT: The positive expression rate of COX-2 and Ki-67 in NIP, NP and NM group was decreased in turn, COX-2 had significant difference in the groups(χ2 = 30.00, P< 0. 05); the positive expression rate of Ki-67 had significant differences between NIP and NM group (χ2 = 8. 533, P<0. 05). The expression of COX-2 in NIP tissues was positively correlate with that of Ki-67 by using Spearman rank correlation analysis (r=0.78, P<0.05). Expression of p21 were not observed in NIP group. The positive rate of HPV was 26. 67% in 30 cases of NIP, all of HPV16 type. CONCLUSION COX-2, Ki-67 and HPV infection have certain correlation with the occurrence of NIP. The occurrence of NIP has relationship with inflammatory reaction mediated by COX-2. Ki-67 can well reflect the proliferation activity of tumor cells, and can be used to measure the proliferation rate of nasal inverted papilloma. The COX-2 and Ki-67 have a synergistic role in the pathogenesis of NIP. p21 has no significant relationship with the incidence of NIP. HPV infection is related to the pathogenesis of NIP, but not as a;major factor in the pathogenesis of NIP.
Case-Control Studies ; Cyclin-Dependent Kinase Inhibitor p21 ; biosynthesis ; Cyclooxygenase 2 ; biosynthesis ; Humans ; Ki-67 Antigen ; biosynthesis ; Nasal Mucosa ; Nasal Polyps ; Nose Neoplasms ; genetics ; virology ; Papilloma, Inverted ; genetics ; virology ; Papillomavirus Infections