Objective To explore the effect and mechanism of electroacupuncture on eNOS in mobilizing endothe -lial progenitor cells (EPCs) in rat bone marrow and peripheral blood to promote revascularization in focal cerebral ischemi -a/reperfusion rat.Methods A total of 100 healthy male adult Sprague-Dawley (SD) rats were randomly divided into nor-mal group ( N) , model group ( I/R) , electroacupuncture group ( I/RE) and I/RE plus L-NAME ( A specific antagonist of eNOS) group (I/REL), and were further divided into 1 d, 2 d and 7 d subgroups after reperfusion, 10 rats in each group, in addition to the N group .The rats received filament occlusion of the right middle cerebral artery for 1.5 hours followed by reperfusion .“Baihui” ( GV 20)/“Siguan” ( Hegu LI 4/Taichong LR 3) were selected as acupucture points .Flow cytome-ter was used to detect the percentage of EPCs in bone marrow and peripheral blood .The expression of VEGFR2 mRNA was tested by fluorescence quantitative PCR .Immunohistochemical staining was used to detect VEGFR 2 +cells and to stain the CD34 +microvessels.Results Compared with the I/R group, there was a significant up-regulation of the percentage of EPCs in bone marrow and peripheral blood by electroacupuncture (P<0.01,P<0.05), but decreased by the inhibitor of eNOS (P<0.01).Compared with the I/R group, the VEGFR2 +cells, expression of VEGFR2 mRNA and CD34 +mi-crovessels were significantly increased in the I/RE group ( P<0.01 ) , but decreased in the I/REL group ( P<0.01 ) . Conclusions Electroacupuncture can effectively mobilize EPCs to promote the revascularization in focal cerebral ischemia /reperfusion rat .This effect is attenuated by inhibitor of eNOS , suggesting that the activation of eNOS mediated by electroa-cupuncture may be related to mobilizing EPCs to promote the revascularization .

Objective:To explore the gene mutations of UGT1A1 * 6 and UGT1A1 * 28 in patients with unconjugated hyperbilirubinemia after renal transplantation and understand their clinical significance.Methods:UGT1A1*6 and UGT1A1*28 gene fragments in blood samples of patients with unconjugated hyperbilirubinemia after renal transplantation were detected by digital fluorescent molecular hybridization sequencing.Results:A total of 21 patients with unconjugated hyperbilirubinemia after renal transplantation were examined for UGT1A1*6 and UGT1A1*28 alleles. The results showed that there were 3 UGT1A1*28 and UGT1A1*6 combined heterozygous mutations, 4 UGT1A1*28 gene heterozygous mutations, 2 UGT1A1*6 heterozygous mutations and 4 UGT1A1*6 homozygous mutations. Among them, the mutation rates of UGT1A1*28 gene and UGT1A1*6 gene were 33%(7/21) and 43%(9/21) respectively and the total mutation rate of both was 62%(13/21).Conclusions:UGT1A1 polymorphism is associated with unconjugated hyperbilirubinemiaafter renal transplantation. By detecting the sequence of UGT1A1*6 and UGT1A1*28 gene fragments in blood samples of renal transplant patients, it is helpful to clarify the etiology of unconjugated hyperbilirubinemia after renal transplantation to confirm the diagnosis of Gilbert syndrome and rule out the effect of immunosuppressive drugs on liver function so as to guide the clinical medication of renal transplant patients.