1.Synthesis and Identification of Hapten and Complete Antigen of Norketa mine
Tingting YANG ; Chuncheng ZHAO ; Xiaoping SHEN ; Bochang TAN ; Pankun ZOU ; Huaiming WANG ; Lingyun LIU ; Chao WEI ; Wanli WEI ; Jinhai HE ; Jie WU ; Dongsheng ZHANG ; Xiaolian ZHAO
Chinese Journal of Analytical Chemistry 2010;38(1):109-112
Under low temperature conditions, the hapten carboxyl-norketamine was synthesized by reacting norketamine and succinaldehyde acid.Identification result using electrospray ionization mass showed the hap ten was successfully synthesized.The artificial antigen confirmed by infrared spectroscopy was developed by conjugating hapten to carrier proteins with carbodiimide(EDC) method.Matrix-assisted laser desorption ioni zation time of flight mass spectrometry(MALDI-TOF-MS) showed that the ratio of hapten to BSA was 11:1.The antibody with high titer(5.12 × 10~4) was produced after immuning to rabbits.
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