OBJECTIVE: To establish a bacterial endotoxin test method for fluorescein sodium injection.METHODS: Based on the bacterial endotoxin test method stated in Chinese Pharmacopoeia 2005 edition(second part),an interference test was conduced on different batches of fluorescein sodium samples using tachypleus amebocyte lysate from different companies.RESULTS: At a concentration of no more than 0.75 mg?mL-1,fluorescein sodium showed no interfere with the bacterial endotoxin test.CONCLUSION: It is feasible for bacterial endotoxin test of fluorescein sodium injection be conducted in a limit value of 0.15 EU?mL-1.

Currently targeted therapy of breast cancer therapy has become the hot research field and a kind of brand-new biological treatment mode after three traditional pattern of the surgery, radiotherapy and chemotherapy for breast cancer. Molecular targeted therapy is aimed at the target that may cause cancer cells, such as protocarcinogenic genes and tumor-suppressor genes, cell signaling pathways, cytokines and receptors, antiangiogenesis etc. It can reverse malignant biology behavior to inhibit tumor cell growth from the molecular level and has the advantages of high specific effects and low side effects. This paper focuses on the drug of the molecular targeted therapy for breast cancer and the latest progress of targeted therapy.

Objective To establish the quality standard for Kangyuan Granula. Methods Radix Astragali, Radix Paeoniae Alba, Fructus Lycii and Radix et Rhizoma Glycyrrhizae in this prescription were identified by TLC. The content of the Paeoniflorin was determined by HPLC. The column of AichromBond-AQ-C18 (4.6 mm?150 mm, 5 ?m) was used, the mobile phase was Water-Acetonitrile (85∶15), at the flow of 1.0 mL/min, the column temperature of 30 ℃, and peaks were detected at 230 nm. Results The characteristic of identification by TLC was distinct and highly specific. The linear range of Paeoniflorin was 0.0545～0.545 ?g, r =0.999 5, the average recovery was 97.90%, RSD=1.06% (n =6). Conclusion The method was easy to operate with accurate result and good reproducibility. It can be used effectively for the quality control of this preparation.

Ischemic stroke is one of the major diseases of causing adult disability and death.Age,sex,smoking,hypertension,and diabetes,etc.are the risk factors for ischemic stroke,but they can only partially explain the reasons of stroke onset.Twins,families,and single-gene genetic stroke studies have shown that ischemic stroke has obvious genetic predisposition.In recent years,genomics has made remarkable progress in ischemic stroke study and has shown that many new single nucleotide polymorphisms or genes are associated with ischemic stroke and its risk factors.However,the contribution of these genetic genes for ischemic stroke is still too small and the repetitive studies are needed for further confirmation.

Hedgehog signaling pathway plays an key role in the process of endochondral bone formation, regulating the proliferation and differentiation of chondrocytes in epiphyseal growth plate. In recent years, it has been found that abnormal expression of Hedgehog signaling pathway-related molecules is involved in a variety of osteoarthropathy. Therefore, studying the relationship between Hedgehog signaling pathway and cartilage damage is of great significance to elucidate the molecular mechanism of osteoarthropathy. In this paper, we focus on the research progress of Hedgehog signaling pathway in chondrocyte proliferation, differentiation, apoptosis and cartilage matrix metabolism, which may provide new insights into mechanism and prevention of osteoarthropathy.

Objective To study the clinical effect of combination therapy of acupuncture and medicine in the treatment of knee osteoarthritis.Methods 500 cases of knee osteoarthritis were studied.250 patients from January to June 2015 were selected as the control group with using moxibustion and Chinese medicine for oral treatment;150 patients from July to December 2015 were selected as observation group with using acupuncture and Chinese herbal medicine for oral treatment.The clinical effect of the two groups of patients were compared.Results The total effective rate of clinical treatment in the observation group was 93.60％,which was significantly higher than 76.80％ the control group,the difference between the two groups was statistically significant (x2 =27.98,P ＜ 0.05).At the same time,the total score of Lequesne and the individual score of the observation group were significantly better than those of the control group (P ＜ 0.05).Conclusion Acupuncture combined with traditional Chinese medicine for oral osteoarthritis in patients with clinical treatment has a significant clinical effect,can effectively improve the patient's knee function,ease the clinical symptoms of patients,and it has the exact effect,it is worthy of clinical promotion.

Objective To study the effectiveness of phase Ⅰleft colon resection and anastomosis on serum insulin-like growth factor-1(IGF-1) and vascular endothelial growth factor (VEGF) in patients with acute intestinal obstruction.Methods 92 patients with acute intestinal obstruction were randomly divided into two groups ,the obser-vation group(n=46) and the control group(n=46).The patients in the observation group were operated through phase Ⅰleft colon resection and anastomosis ,while the patients in the control group were operated through Hartman . Serum IGF-1 and VEGF were measured in preoperative and postoperative patients .Results The observation group for the length of time (14.9 ±6.4)d,shorter than the control group(28.3 ±9.6)d(t=5.426,P<0.05);observation group,incision infection,anastomotic leakage were 2 cases,1 cases,the control group was 3 cases,1 cases,no signifi-cant difference between two groups (χ2 =2.19,1.29,all P>0.05);two groups of postoperative serum levels of IGF-1 and VEGF were significantly decreased ,compared with the control group ,the observation group was significantly lower (t=3.064,2.394,all P<0.05).Conclusion Phase Ⅰ left colon resection and anastomosis can significantly de-crease serum IGF-1 and VEGF levels in patients with acute intestinal obstruction .

Objective To investigate the occurrence and distribution characteristics of pathogenic fungi causing su-perficial mycosis in a hospital. Methods Specimens from patients suspected of superficial mycosis were conducted direct microscope examination,cultivation and pathogenic identification.Results Among 398 cases of suspected su-perficial mycosis,the positive rate of fungi was 51.01% (n= 203 )by direct microscopy and 59.55% (n= 237)by fungi cultivation,positive rate of microscope examination was significantly lower than cultivation (χ2= 6.057,P=0.014). Of 237 patients with confirmed diagnosis of superficial mycosis ,100 (42.19% )were children and 137 (57.81% )were adults ;109 cases (45.99% )were diagnosed with tinea of feet and hands,89 (37.55% )tinea capi-tis,26 (10.97% )tinea corporis,and 13 (5.48% )tinea unguium. The major fungi from children was Microsporum canis (49.00% ),and from adults was Trichophytonrubrum(45.99% ). Conclusion The most common superficial mycosis in this hospital is tinea of feet and hands,and the major fungi are Trichophytonrubrum and Microsporum canis ,the species causing mycosis in adults and children are different.

Objective To analyze and summarize the clinicopathological features of recurrent uterine Mullein adenofibroma . Methods 8 cases of recurrent uterine Mullein adenofibroma in our hospital from February 2003 to February 2014 were selected and analyzed on the clinical data for summarizing its clinicopathological features .Results In 8 cases of recurrent uterine M ullerian ade‐nofibroma ,the lobulated neoplasm could be found on outside of the cervix or inside of the uterus ,which was formed by the benign epithelial and mesenchymal tissue .Immunohistochemistry showed that the estrogen receptor (ER) was expressed in 8 cases ,some cases expressed progesterone receptor(PR) ,smooth muscle actin(SMA) ,CD10 and p53 ,and the mean average value of Ki‐67 (+ ) was 8% .Conclusion Recurrent uterine Mullerian adenofibroma has no specific clinicopathological changes ,its differential diagnosis with sarcoma is difficult .Only CD10 and Ki‐67 may be conducive to diagnosis .

The hospital ship is an important force in medical support. The normal use of medical equipment is prerequisite for effective clinical work. According to the particularity of nautical work, different ways of fixation and expasion with various medical equipments in the warship are proposed in order to satisfy the needs of clinical work.