Currently targeted therapy of breast cancer therapy has become the hot research field and a kind of brand-new biological treatment mode after three traditional pattern of the surgery, radiotherapy and chemotherapy for breast cancer. Molecular targeted therapy is aimed at the target that may cause cancer cells, such as protocarcinogenic genes and tumor-suppressor genes, cell signaling pathways, cytokines and receptors, antiangiogenesis etc. It can reverse malignant biology behavior to inhibit tumor cell growth from the molecular level and has the advantages of high specific effects and low side effects. This paper focuses on the drug of the molecular targeted therapy for breast cancer and the latest progress of targeted therapy.

Ischemic stroke is one of the major diseases of causing adult disability and death.Age,sex,smoking,hypertension,and diabetes,etc.are the risk factors for ischemic stroke,but they can only partially explain the reasons of stroke onset.Twins,families,and single-gene genetic stroke studies have shown that ischemic stroke has obvious genetic predisposition.In recent years,genomics has made remarkable progress in ischemic stroke study and has shown that many new single nucleotide polymorphisms or genes are associated with ischemic stroke and its risk factors.However,the contribution of these genetic genes for ischemic stroke is still too small and the repetitive studies are needed for further confirmation.

OBJECTIVE: To establish a bacterial endotoxin test method for fluorescein sodium injection.METHODS: Based on the bacterial endotoxin test method stated in Chinese Pharmacopoeia 2005 edition(second part),an interference test was conduced on different batches of fluorescein sodium samples using tachypleus amebocyte lysate from different companies.RESULTS: At a concentration of no more than 0.75 mg?mL-1,fluorescein sodium showed no interfere with the bacterial endotoxin test.CONCLUSION: It is feasible for bacterial endotoxin test of fluorescein sodium injection be conducted in a limit value of 0.15 EU?mL-1.

Objective To establish the quality standard for Kangyuan Granula. Methods Radix Astragali, Radix Paeoniae Alba, Fructus Lycii and Radix et Rhizoma Glycyrrhizae in this prescription were identified by TLC. The content of the Paeoniflorin was determined by HPLC. The column of AichromBond-AQ-C18 (4.6 mm?150 mm, 5 ?m) was used, the mobile phase was Water-Acetonitrile (85∶15), at the flow of 1.0 mL/min, the column temperature of 30 ℃, and peaks were detected at 230 nm. Results The characteristic of identification by TLC was distinct and highly specific. The linear range of Paeoniflorin was 0.0545～0.545 ?g, r =0.999 5, the average recovery was 97.90%, RSD=1.06% (n =6). Conclusion The method was easy to operate with accurate result and good reproducibility. It can be used effectively for the quality control of this preparation.

OBJECTIVE: To investigate and analyze the epidemiological and etiological characteristics of exercise-related sudden death, so as to provide scientific and effective preventive measures for competitive sports and the body building of the entire people.DATA SOURCES: A computer-based online search of Medline database was conducted to identify articles about exercise-related sudden death published in English between January 1982 and July 2005 by using the keywords of "exercise/sport, sudden death". Meanwhile, Chinese relevant articles at the same period were searched in VIP database with the same keywords in Chinese.STUDY SELECTION: The data were primarily checked, those about exercise-related sudden death were selected, and the repetitive studies and case report were deleted.DATA EXTRACTION: Fourteen of the 63 literatures were involved after classification.DATA SYNTHESIS: Exercise-related sudden death has low incidence and rapid progress, and it has no obvious correlation with the event and intensity of sports, it occurs more in males than in females of 30-60 years.Most of the etiological factors are sudden cardiac death and sudden cerebral death, and coronary atherosclerosis is one of the main causes for exercise-related sudden death.CONCLUSION: Exercise-related sudden death has low incidence, and there is sex characteristics, coronary atherosclerosis is an important factor for exercise-related sudden death. Studies on the epidemiology and etiology of exercise-related sudden death, as well as the preventive measures,should be reinforced.

hs-cTn was proved to has good clinical application value. The assay has physiological significance because hs-cTn can be measured in apparently healthy subjects. There is no consensus on the establishing 99th percentile values and choosing the critical values for different populations based on the age,sex or ethnicity.

Objective To observe the effect and safety of orthokeratology in myopia patients.Methods Sixty-eight(136 eyes)young patients with myopia wore orthokeratology lens as research group,and 55 cases(110 eyes)young patients with myopia wore glasses as control group,clinical data of both groups were retrospectively analyzed to evaluate the role of orthokeratology lens on controlling the progression of myopia.Results After treatment,uncorrected visual acuity of research group significantly improved (0.76 ± 0.15 vs.0.40 ± 0.13,P ＜ 0.05),while spherical equivalent(SE)and corneal curvature significantly reduced[(-2.63 ± 1.06)D vs.(-3.52 ± 1.25)D,(42.30 ± 1.25)D vs.(43.21 ± 1.28)D,P ＜ 0.05].After treatment,SE of control group significantly increased[(-4.65 ± 1.29)D vs.(-3.55 ± 1.27)D,P ＜ 0.05],axis oculi significantly prolonged[(25.16 ± 0.55)mm vs.(24.30 ± 0.53)mm,P ＜ 0.05].Research group had no serious corneal infection happened during the treatment.Conclusion Orthokeratology lens can effectively reduce the degree of myopia and slow down the axis oculi growth,and it is used convenient with high security.

Epithelial-mesenchymal transition (EMT) occurs as a basic physiological process during the embryonic development. The latest studies find that EMT also plays an important role in the progression of tumors invasion and metastasis. In the course of tumor progression, EMT involves a variety of molecular mechanisms and complex networks. Thus,the in-depth research of EMT mechanisms and markers have a high prognostic and therapeutic value in aggressive cancers.

Objective To investigate the application of laparoscopic cholecystectomy(LC) in the liver,cross ditch right notch identification,prevention of bile duct injury anatomy of cystic duct.Methods 224 cases with liver transverse groove had right notch,all operations in the liver transverse groove right notch on the separation of anatomic posterior Calot's triangle LC operation.Results 224 cases of LC were performed smoothly,the entire group without the occurrence of bile duct injury,hemorrhage,conversion to open surgery,postoperative all patients recovered were well cured.Conclusion Liver transverse groove notch on the right posterior Calot's triangle anatomic helps to avoid LC operation structure and variation of extrahepatic bile duct,which can effectively prevent the biliary duct injury.

Objective To evaluate the clinical efficacy of flupentixol melitracen joint esomeprazole, mosapride in the treatment of refractory gastroesophageal reflux disease.Methods 80 patients with refractory gastroe-sophageal reflux disease were randomly divided into control group and observation group,40 cases in each group.Two groups of patients were given oral esomeprazole and mosapride treatment,the observation group was treated with flu-pentixol melitracen on the basis of control group.4 weeks for a course of treatment,patients were treated for 2 courses. The clinical efficacy of the two groups,the depression and anxiety RDQ score before and after treatment,the adverse reactions were recorded.Results The total effective rate of the observation group was 82.50%,which was signifi-cantly higher than 70.00% of the control group,the difference was statistically significant (χ2 =3.660,P <0.05). Before treatment,SAS,SDS score and RDQ level between the two groups had no significant difference (all P >0.05). After treatment,the RDQ in symptom score and frequency integrator of the control group were (8.12 ±0.89)points, (7.70 ±1.00)points,compared with before treatment,the differences were significant (t =4.70,6.37,all P <0.05),and those of the observation group were (5.21 ±0.45)points,(4.71 ±0.78)points,which were significantly lower than those of the control group,the differences were statistically significant (t =1.81,1.81,all P <0.05).After treatment,the SAS score and SDS score of control group were (49.12 ±5.89)points,(50.30 ±6.00)points,com-pared with before treatment,the differences were significant (t =1.81,1.81,all P <0.05),and those of the observa-tion group were (41.21 ±5.45),(43.71 ±5.78),which were significantly lower than the control group (t =2.30, 1.91,all P <0.05).During the treatment,the adverse events in the two groups were similar,no serious adverse reac-tions were observed.Conclusion Flupentixol melitracen joint esomeprazole,mosapride in the treatment of refractory gastroesophageal reflux disease has significant clinical effect,can effectively improve the clinical symptoms of depres-sion and anxiety scores,and without significant adverse reactions,it is worthy of clinical application.