Ischemic stroke is one of the major diseases of causing adult disability and death.Age,sex,smoking,hypertension,and diabetes,etc.are the risk factors for ischemic stroke,but they can only partially explain the reasons of stroke onset.Twins,families,and single-gene genetic stroke studies have shown that ischemic stroke has obvious genetic predisposition.In recent years,genomics has made remarkable progress in ischemic stroke study and has shown that many new single nucleotide polymorphisms or genes are associated with ischemic stroke and its risk factors.However,the contribution of these genetic genes for ischemic stroke is still too small and the repetitive studies are needed for further confirmation.

OBJECTIVE: To establish a bacterial endotoxin test method for fluorescein sodium injection.METHODS: Based on the bacterial endotoxin test method stated in Chinese Pharmacopoeia 2005 edition(second part),an interference test was conduced on different batches of fluorescein sodium samples using tachypleus amebocyte lysate from different companies.RESULTS: At a concentration of no more than 0.75 mg?mL-1,fluorescein sodium showed no interfere with the bacterial endotoxin test.CONCLUSION: It is feasible for bacterial endotoxin test of fluorescein sodium injection be conducted in a limit value of 0.15 EU?mL-1.

Objective To establish the quality standard for Kangyuan Granula. Methods Radix Astragali, Radix Paeoniae Alba, Fructus Lycii and Radix et Rhizoma Glycyrrhizae in this prescription were identified by TLC. The content of the Paeoniflorin was determined by HPLC. The column of AichromBond-AQ-C18 (4.6 mm?150 mm, 5 ?m) was used, the mobile phase was Water-Acetonitrile (85∶15), at the flow of 1.0 mL/min, the column temperature of 30 ℃, and peaks were detected at 230 nm. Results The characteristic of identification by TLC was distinct and highly specific. The linear range of Paeoniflorin was 0.0545～0.545 ?g, r =0.999 5, the average recovery was 97.90%, RSD=1.06% (n =6). Conclusion The method was easy to operate with accurate result and good reproducibility. It can be used effectively for the quality control of this preparation.

Currently targeted therapy of breast cancer therapy has become the hot research field and a kind of brand-new biological treatment mode after three traditional pattern of the surgery, radiotherapy and chemotherapy for breast cancer. Molecular targeted therapy is aimed at the target that may cause cancer cells, such as protocarcinogenic genes and tumor-suppressor genes, cell signaling pathways, cytokines and receptors, antiangiogenesis etc. It can reverse malignant biology behavior to inhibit tumor cell growth from the molecular level and has the advantages of high specific effects and low side effects. This paper focuses on the drug of the molecular targeted therapy for breast cancer and the latest progress of targeted therapy.

Objective To observe the effect and safety of orthokeratology in myopia patients.Methods Sixty-eight(136 eyes)young patients with myopia wore orthokeratology lens as research group,and 55 cases(110 eyes)young patients with myopia wore glasses as control group,clinical data of both groups were retrospectively analyzed to evaluate the role of orthokeratology lens on controlling the progression of myopia.Results After treatment,uncorrected visual acuity of research group significantly improved (0.76 ± 0.15 vs.0.40 ± 0.13,P ＜ 0.05),while spherical equivalent(SE)and corneal curvature significantly reduced[(-2.63 ± 1.06)D vs.(-3.52 ± 1.25)D,(42.30 ± 1.25)D vs.(43.21 ± 1.28)D,P ＜ 0.05].After treatment,SE of control group significantly increased[(-4.65 ± 1.29)D vs.(-3.55 ± 1.27)D,P ＜ 0.05],axis oculi significantly prolonged[(25.16 ± 0.55)mm vs.(24.30 ± 0.53)mm,P ＜ 0.05].Research group had no serious corneal infection happened during the treatment.Conclusion Orthokeratology lens can effectively reduce the degree of myopia and slow down the axis oculi growth,and it is used convenient with high security.

Locally advanced non-small cell lung cancer (LA-NSCLC) usually is inoperable because of tumor progress.At present,concurrent chemoradiotherapy has become standard regimen of these patients,which has gotten remarkable effectiveness.This article is a summarization on concurrent chemoradiotherapy for LA-NSCLC.

Objective To evaluate the iron metabolism in host-pathogen interplay.MethodsA total of 13 expression profiles of iron metabolism related genes of RAW264.7 murine macrophages uninfected or infected with Salmonella typhimurium were stuldied by real-time PCR.Results The live wild-type Salmonella typhimurium induced the expression of the transferrin receptor(Tfr1)in host cell macrophages,which resulted in a sustained increase of the labile iron pool inside the host oell 1h or 24h after infection.Gene expression analysis showed that wild-type Salmonella typhimurium drove an active iron acquisition program with induction of ferrireductase(Steap3),iron membrane transporter Dmtl,and iron regulatory proteins(Irpl and Irp2),and a little of iron efflux change through ferriportin(Fpnl).On the other hand,the Salmonella mutant strain spiC-used in present studies did not cause an increase mRNA level for Tfrl at 1h or 24h,but led to an increase mRNA level for Epnl at 24h as compared with 1h. The assessment was that the labile iron pool decreased after infection with spiC-Salmonella for 24h.Conclusion Wild-type Salmonella typhimurium appears to drive an active transferrin-mediated iron uptake program after infection 1h or 24h.The spiC-Salmonella mutant strain used in our studies shows an increased iron efflux from infected cells at 24h as comparde with 1h.

OBJECTIVE: To investigate and analyze the epidemiological and etiological characteristics of exercise-related sudden death, so as to provide scientific and effective preventive measures for competitive sports and the body building of the entire people.DATA SOURCES: A computer-based online search of Medline database was conducted to identify articles about exercise-related sudden death published in English between January 1982 and July 2005 by using the keywords of "exercise/sport, sudden death". Meanwhile, Chinese relevant articles at the same period were searched in VIP database with the same keywords in Chinese.STUDY SELECTION: The data were primarily checked, those about exercise-related sudden death were selected, and the repetitive studies and case report were deleted.DATA EXTRACTION: Fourteen of the 63 literatures were involved after classification.DATA SYNTHESIS: Exercise-related sudden death has low incidence and rapid progress, and it has no obvious correlation with the event and intensity of sports, it occurs more in males than in females of 30-60 years.Most of the etiological factors are sudden cardiac death and sudden cerebral death, and coronary atherosclerosis is one of the main causes for exercise-related sudden death.CONCLUSION: Exercise-related sudden death has low incidence, and there is sex characteristics, coronary atherosclerosis is an important factor for exercise-related sudden death. Studies on the epidemiology and etiology of exercise-related sudden death, as well as the preventive measures,should be reinforced.

The incidence of bronchial asthma in children is increasing nowadays.More and more studies have showed that epigenetics plays an important role in the pathogenesis of asthma.One of them are histone modification(acetylation and deacetylation),which is catalyzed by histone acetyltransferase (HAT) and histone deacetylase (HDAC).HAT and HDAC can mediate asthma via causing Th1/Th2 imbalance,increased airway hyperresponsiveness,regulation of various inflammatory medium and so on.This paper reviews the HAT and HDAC mediated incidence of asthma and other possible mechanisms,the latest of glucocorticoid in the treatment of children with asthma epigenetic mechanisms.

Kawasaki disease,also known as mucocutaneous lymph node syndrome,is an acute,febrile,multisystem disease in children.The coronary artery lesions caused by Kawasaki disease has become the most common acquired heart disease in children.In recent years,epidemiological investigation on Kawasaki disease has been launched in the world.It can provide the basis for clinical diagnosis and treatment of Kawasaki disease through summarizing the epidemiological characteristics of Kawasaki disease in the different regions in China in recent 20 years,including the characteristics of incidence,age,gender,nationality,family history,seasonal onset,relapse rate,mortality and heart damage.