Currently targeted therapy of breast cancer therapy has become the hot research field and a kind of brand-new biological treatment mode after three traditional pattern of the surgery, radiotherapy and chemotherapy for breast cancer. Molecular targeted therapy is aimed at the target that may cause cancer cells, such as protocarcinogenic genes and tumor-suppressor genes, cell signaling pathways, cytokines and receptors, antiangiogenesis etc. It can reverse malignant biology behavior to inhibit tumor cell growth from the molecular level and has the advantages of high specific effects and low side effects. This paper focuses on the drug of the molecular targeted therapy for breast cancer and the latest progress of targeted therapy.

OBJECTIVE: To establish a bacterial endotoxin test method for fluorescein sodium injection.METHODS: Based on the bacterial endotoxin test method stated in Chinese Pharmacopoeia 2005 edition(second part),an interference test was conduced on different batches of fluorescein sodium samples using tachypleus amebocyte lysate from different companies.RESULTS: At a concentration of no more than 0.75 mg?mL-1,fluorescein sodium showed no interfere with the bacterial endotoxin test.CONCLUSION: It is feasible for bacterial endotoxin test of fluorescein sodium injection be conducted in a limit value of 0.15 EU?mL-1.

Objective To establish the quality standard for Kangyuan Granula. Methods Radix Astragali, Radix Paeoniae Alba, Fructus Lycii and Radix et Rhizoma Glycyrrhizae in this prescription were identified by TLC. The content of the Paeoniflorin was determined by HPLC. The column of AichromBond-AQ-C18 (4.6 mm?150 mm, 5 ?m) was used, the mobile phase was Water-Acetonitrile (85∶15), at the flow of 1.0 mL/min, the column temperature of 30 ℃, and peaks were detected at 230 nm. Results The characteristic of identification by TLC was distinct and highly specific. The linear range of Paeoniflorin was 0.0545～0.545 ?g, r =0.999 5, the average recovery was 97.90%, RSD=1.06% (n =6). Conclusion The method was easy to operate with accurate result and good reproducibility. It can be used effectively for the quality control of this preparation.

Ischemic stroke is one of the major diseases of causing adult disability and death.Age,sex,smoking,hypertension,and diabetes,etc.are the risk factors for ischemic stroke,but they can only partially explain the reasons of stroke onset.Twins,families,and single-gene genetic stroke studies have shown that ischemic stroke has obvious genetic predisposition.In recent years,genomics has made remarkable progress in ischemic stroke study and has shown that many new single nucleotide polymorphisms or genes are associated with ischemic stroke and its risk factors.However,the contribution of these genetic genes for ischemic stroke is still too small and the repetitive studies are needed for further confirmation.

Objective To explore the clinical value of early arterial phase (EAP) enhanced imaging with MSCT in detecting liver focal lesions.Methods 100 patients with liver focal lesions were examined with MSCT scan.The images were analyzed.Results There were 85.0％ of the 100 cases that demonstrated EAP enhancement,which was hemangioma of liver(100.0％),hepatapostema (100.0％),hepatocellular carcinoma (95.0％),liver metastasis (84.0％),and cholangiocellular carcinoma(25.0％) in descending order.The abnormal vascular lesions and heterogeneous enhancement had high sensitivity and specificity for hepatocellular carcinoma,and the edge nodular enhancement for hemangioma,and the ring enhancement for metastases.Conclusion The EAP enhanced imaging with MSCT in detecting liver focal lesions has great applicative value.

Medical institutes in the United States,the United Kingdom and Canada have systematically explored and published standard of physician competence,such as ACGME six-critical-components,GMC good medical practice and CanMEDS,which has led to changes in the content,item format and clinical skill test of foreign medical incensing exam.Foreign research inspires Chinese counterparts to study and develop the Chinese standard of physician competence so as to continuously enhance the medical licensing exam.

Putting emphasis on medical science education and looking down on medical humanistic education is the situation in medical colleges.Reasons of absence of medical humanistic education are as follows,one is the insufficient understanding of medical humanism and the other is the lack of practice.Learning traditional culture can make up for the deficiency of medical humanities education.Caring life and humanitarian spirit is the main value of traditional culture and expounding medical humanitarian spirit plays an important part in medical humanities education.Traditional culture education will cultivate student's virtue,open up the wisdom and construct the harmonious spiritual home.

Objective To evaluate the value of galectin-3 (Gal-3) in the diagnosis and prognosis of heart failure.Methods Sixty-eight patients with heart failure (heart failure group) and 68 healthy person (control group) were selected.The level of Gal-3 and the incidence of cardiovascular event after 6 months follow-up was compared between two groups and among different cardiac functional grading.Results The level of Gal-3 was (44.57 ± 10.42) ng/L in heart failure group,(33.76 ± 8.18) ng/L in control group,and there was significant difference between two groups (P ＜ 0.01).The level of Gal-3 and the incidence of cardiovascular event after 6 months follow-up in cardiac functional grading Ⅳ (15 cases) was higher than that in cardiac functional grading H (32 cases),Ⅲ (21 cases)[(46.45 ± 12.21) ng/L vs.(38.47 ±9.38),(42.31 ± 10.23) ng/L,8/15 vs.9.4％(3/32),28.6％(6/21)],and there was significant difference (P ＜0.01).Conclusion As the marker of heart failure,the Gal-3 has significantly clinical value on diagnosis and prognosis to segment heart failure.

The incidence of bronchial asthma in children is increasing nowadays.More and more studies have showed that epigenetics plays an important role in the pathogenesis of asthma.One of them are histone modification(acetylation and deacetylation),which is catalyzed by histone acetyltransferase (HAT) and histone deacetylase (HDAC).HAT and HDAC can mediate asthma via causing Th1/Th2 imbalance,increased airway hyperresponsiveness,regulation of various inflammatory medium and so on.This paper reviews the HAT and HDAC mediated incidence of asthma and other possible mechanisms,the latest of glucocorticoid in the treatment of children with asthma epigenetic mechanisms.

Kawasaki disease,also known as mucocutaneous lymph node syndrome,is an acute,febrile,multisystem disease in children.The coronary artery lesions caused by Kawasaki disease has become the most common acquired heart disease in children.In recent years,epidemiological investigation on Kawasaki disease has been launched in the world.It can provide the basis for clinical diagnosis and treatment of Kawasaki disease through summarizing the epidemiological characteristics of Kawasaki disease in the different regions in China in recent 20 years,including the characteristics of incidence,age,gender,nationality,family history,seasonal onset,relapse rate,mortality and heart damage.